(Circular) (Six-base) MAPSORT of: pFN21AE6737.seq Check: 6545 from: 1 to: 7303 pFN21AE6737 7303 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5113 278 Size: 53 83 186 936 3577 2468 Fragments arranged by size: 3577 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4646 1937 Size: 2709 4594 Acc65I G'GTAC_C Cuts at: 4624 4624 Size: 7303 AclI AA'CG_TT Cuts at: 1563 5430 5803 1563 Size: 3867 373 3063 Fragments arranged by size: 3867 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2862 3911 3963 4159 4559 5366 6750 7018 Size: 1230 1049 52 196 400 807 1384 268 Cuts at: 7018 1632 Size: 1917 Fragments arranged by size: 1917 1384 1230 1049 807 400 268 196 52 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7106 AflIII A'CryG_T Cuts at: 1203 3711 6202 7179 1203 Size: 2508 2491 977 1327 Fragments arranged by size: 2508 2491 1327 977 AgeI A'CCGG_T Cuts at: 6134 6134 Size: 7303 AhdI GACnn_n'nnGTC Cuts at: 6032 6032 Size: 7303 AleI CACnn'nnGTG Cuts at: 1166 3216 1166 Size: 2050 5253 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7303 AlwNI CAG_nnn'CTG Cuts at: 2029 3243 3934 4182 5046 6618 2029 Size: 1214 691 248 864 1572 2714 Fragments arranged by size: 2714 1572 1214 864 691 248 ApaI G_GGCC'C Cuts at: 3156 3156 Size: 7303 ApaLI G'TGCA_C Cuts at: 5360 6516 5360 Size: 1156 6147 ApoI r'AATT_y Cuts at: 1493 2441 2508 4612 4838 4990 7099 1493 Size: 948 67 2104 226 152 2109 1697 Fragments arranged by size: 2109 2104 1697 948 226 152 67 AseI AT'TA_AT Cuts at: 160 1032 5857 160 Size: 872 4825 1606 Fragments arranged by size: 4825 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7303 AvaI C'yCGr_G Cuts at: 1870 1943 2072 4628 1870 Size: 73 129 2556 4545 Fragments arranged by size: 4545 2556 129 73 BamHI G'GATC_C Cuts at: 3246 3286 4633 5000 3246 Size: 40 1347 367 5549 Fragments arranged by size: 5549 1347 367 40 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2076 2633 4624 6079 618 Size: 325 217 627 289 557 1991 1455 1842 Fragments arranged by size: 1991 1842 1455 627 557 325 289 217 BanII G_rGCy'C Cuts at: 1391 2069 2256 2761 3156 3426 3563 4622 1391 Size: 678 187 505 395 270 137 1059 4072 Fragments arranged by size: 4072 1059 678 505 395 270 187 137 BbeI G_GCGC'C Cuts at: 2080 2080 Size: 7303 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 4321 928 Size: 971 2422 3910 Fragments arranged by size: 3910 2422 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5494 5528 1903 Size: 34 50 34 3473 34 3678 Fragments arranged by size: 3678 3473 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5195 6405 5195 Size: 1210 6093 BclI T'GATC_A Cuts at: 1548 1548 Size: 7303 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5914 136 Size: 107 122 71 631 605 4242 1525 Fragments arranged by size: 4242 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3946 7298 3946 Size: 3352 3951 BlpI GC'TnA_GC Cuts at: 3741 4720 3741 Size: 979 6324 Bme1580I G_kGCm'C Cuts at: 1790 2638 3156 5364 6520 1790 Size: 848 518 2208 1156 2573 Fragments arranged by size: 2573 2208 1156 848 518 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7303 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3187 3313 5034 Size: 416 53 83 399 24 1770 126 1721 Cuts at: 5034 5992 442 Size: 958 1753 Fragments arranged by size: 1770 1753 1721 958 416 399 126 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7303 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3423 3423 Size: 7303 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2261 2589 2979 3458 4184 5963 1239 Size: 1022 328 390 479 726 1779 2579 Fragments arranged by size: 2579 1779 1022 726 479 390 328 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5428 6293 6591 5428 Size: 865 298 6140 Fragments arranged by size: 6140 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5966 882 Size: 617 4467 2219 Fragments arranged by size: 4467 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3123 5094 493 Size: 711 1919 1971 2702 Fragments arranged by size: 2702 1971 1919 711 BsaBI GATnn'nnATC Cuts at: 3030 3291 4209 4350 7297 3030 Size: 261 918 141 2947 3036 Fragments arranged by size: 3036 2947 918 261 141 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2077 5110 5492 6127 Size: 53 83 186 936 544 3033 382 635 Cuts at: 6127 275 Size: 1451 Fragments arranged by size: 3033 1451 936 635 544 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2803 5736 6134 6408 6555 6966 2803 Size: 2933 398 274 147 411 3140 Fragments arranged by size: 3140 2933 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3112 3142 3431 3461 1178 Size: 30 165 30 1709 30 289 30 5020 Fragments arranged by size: 5020 1709 289 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 4130 1186 Size: 2944 4359 BseYI C'CCAG_C Cuts at: 2615 3395 3471 3902 4042 4519 6506 7002 2615 Size: 780 76 431 140 477 1987 496 2916 Fragments arranged by size: 2916 1987 780 496 477 431 140 76 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2637 1163 Size: 573 901 5829 Fragments arranged by size: 5829 901 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2098 4753 5514 6542 1540 Size: 275 186 97 2655 761 1028 2301 Fragments arranged by size: 2655 2301 1028 761 275 186 97 BsiHKAI G_wGCw'C Cuts at: 2256 2877 5364 5449 6520 2256 Size: 621 2487 85 1071 3039 Fragments arranged by size: 3039 2487 1071 621 85 BsmI GAATG_Cn' Cuts at: 2746 4108 4156 4814 4907 2746 Size: 1362 48 658 93 5142 Fragments arranged by size: 5142 1362 658 93 48 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2256 2638 2761 2877 3156 3426 Size: 399 279 187 382 123 116 279 270 Cuts at: 3426 3563 4622 5364 5449 6520 1391 Size: 137 1059 742 85 1071 2174 Fragments arranged by size: 2174 1071 1059 742 399 382 279 279 270 187 137 123 116 85 BspEI T'CCGG_A Cuts at: 6966 6966 Size: 7303 BspHI T'CATG_A Cuts at: 3808 4098 5192 6922 3808 Size: 290 1094 1730 4189 Fragments arranged by size: 4189 1730 1094 290 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2273 4658 844 Size: 1429 2385 3489 Fragments arranged by size: 3489 2385 1429 BsrBI CCG'CTC Cuts at: 1394 1823 5190 1394 Size: 429 3367 3507 Fragments arranged by size: 3507 3367 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5798 5972 65 Size: 1183 4550 174 1396 Fragments arranged by size: 4550 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4569 5947 6134 1700 Size: 110 2759 1378 187 2869 Fragments arranged by size: 2869 2759 1378 187 110 BsrGI T'GTAC_A Cuts at: 96 4462 96 Size: 4366 2937 BssHII G'CGCG_C Cuts at: 1925 2019 1925 Size: 94 7209 BssSI C'ACGA_G Cuts at: 2760 5363 6375 2760 Size: 2603 1012 3688 Fragments arranged by size: 3688 2603 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2029 2612 794 Size: 1235 583 5485 Fragments arranged by size: 5485 1235 583 BstBI TT'CG_AA Cuts at: 6107 6107 Size: 7303 BstEII G'GTnAC_C Cuts at: 1184 2977 1184 Size: 1793 5510 BstXI CCAn_nnnn'nTGG Cuts at: 3067 3218 3767 3067 Size: 151 549 6603 Fragments arranged by size: 6603 549 151 BstYI r'GATC_y Cuts at: 1972 2264 3246 3286 3946 4633 5000 5385 5402 Size: 292 982 40 660 687 367 385 17 Cuts at: 5402 6854 6940 6952 7298 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 982 687 660 385 367 346 292 86 40 17 12 BtgI C'CryG_G Cuts at: 513 1065 3060 3453 513 Size: 552 1995 393 4363 Fragments arranged by size: 4363 1995 552 393 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6564 Fragments arranged by size: 6564 683 56 BtsI GCAGTG_nn' Cuts at: 4011 4819 5613 5633 4011 Size: 808 794 20 5681 Fragments arranged by size: 5681 808 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7303 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7268 DraI TTT'AAA Cuts at: 2446 2643 4607 2446 Size: 197 1964 5142 Fragments arranged by size: 5142 1964 197 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2975 6310 809 Size: 507 1659 3335 1802 Fragments arranged by size: 3335 1802 1659 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2095 2208 4750 Size: 54 1425 50 275 18 265 113 2542 Cuts at: 4750 5639 7268 8 Size: 889 1629 43 Fragments arranged by size: 2542 1629 1425 889 275 265 113 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2095 4750 1537 Size: 275 283 2655 4090 Fragments arranged by size: 4090 2655 283 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4060 4430 5233 1353 Size: 2707 370 803 3423 Fragments arranged by size: 3423 2707 803 370 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5890 6264 6410 387 Size: 1064 138 4301 374 146 1280 Fragments arranged by size: 4301 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2254 2254 Size: 7303 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2261 2589 2862 2979 3458 3911 3963 Size: 393 629 328 273 117 479 453 52 Cuts at: 3963 4159 4184 4559 5366 5963 6750 7018 1239 Size: 196 25 375 807 597 787 268 1524 Fragments arranged by size: 1524 807 787 629 597 479 453 393 375 328 273 268 196 117 52 25 EcoO109I rG'GnC_Cy Cuts at: 2035 3152 3153 3922 2035 Size: 1117 1 769 5416 Fragments arranged by size: 5416 1117 769 1 EcoRI G'AATT_C Cuts at: 4612 4990 4612 Size: 378 6925 EcoRV GAT'ATC Cuts at: 4026 4026 Size: 7303 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3031 3031 Size: 7303 FspI TGC'GCA Cuts at: 5809 5809 Size: 7303 HaeII r_GCGC'y Cuts at: 1678 2080 4085 6450 1678 Size: 402 2005 2365 2531 Fragments arranged by size: 2531 2365 2005 402 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3739 3771 5944 5976 6018 Size: 32 135 32 2167 32 2173 32 42 Cuts at: 6018 6050 1373 Size: 32 2626 Fragments arranged by size: 2626 2173 2167 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3717 4647 4893 669 Size: 988 281 1779 930 246 3079 Fragments arranged by size: 3079 1779 988 930 281 246 HindIII A'AGCT_T Cuts at: 748 2850 748 Size: 2102 5201 HpaI GTT'AAC Cuts at: 3717 4893 3717 Size: 1176 6127 KasI G'GCGC_C Cuts at: 2076 2076 Size: 7303 KpnI G_GTAC'C Cuts at: 4628 4628 Size: 7303 MfeI C'AATT_G Cuts at: 4902 4902 Size: 7303 MluI A'CGCG_T Cuts at: 7179 7179 Size: 7303 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2929 4023 6417 6601 1305 Size: 1624 1094 2394 184 2007 Fragments arranged by size: 2394 2007 1624 1094 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2210 10 Size: 54 1425 343 378 5103 Fragments arranged by size: 5103 1425 378 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2817 3065 3216 5261 5620 5779 518 Size: 648 1651 248 151 2045 359 159 2042 Fragments arranged by size: 2045 2042 1651 648 359 248 159 151 MspA1I CmG'CkG Cuts at: 1606 2026 2059 4719 5396 6544 6789 1606 Size: 420 33 2660 677 1148 245 2120 Fragments arranged by size: 2660 2120 1148 677 420 245 33 NarI GG'CG_CC Cuts at: 2077 2077 Size: 7303 NcoI C'CATG_G Cuts at: 513 1065 3060 3453 513 Size: 552 1995 393 4363 Fragments arranged by size: 4363 1995 552 393 NdeI CA'TA_TG Cuts at: 387 2938 3349 4453 387 Size: 2551 411 1104 3237 Fragments arranged by size: 3237 2551 1104 411 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7303 NotI GC'GGCC_GC Cuts at: 4750 4750 Size: 7303 NspI r_CATG'y Cuts at: 1132 1229 2477 3715 4661 6206 1132 Size: 97 1248 1238 946 1545 2229 Fragments arranged by size: 2229 1545 1248 1238 946 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7303 PciI A'CATG_T Cuts at: 3711 6202 3711 Size: 2491 4812 PflMI CCAn_nnn'nTGG Cuts at: 1108 3002 1108 Size: 1894 5409 PfoI T'CCnGG_A Cuts at: 2305 2566 2305 Size: 261 7042 PmeI GTTT'AAAC Cuts at: 4607 4607 Size: 7303 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2475 2507 2781 2813 5372 5404 6885 6917 2475 Size: 32 274 32 2559 32 1481 32 2861 Fragments arranged by size: 2861 2559 1481 274 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3922 3922 Size: 7303 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7303 PsiI TTA'TAA Cuts at: 4873 4873 Size: 7303 PspOMI G'GGCC_C Cuts at: 3152 3152 Size: 7303 PstI C_TGCA'G Cuts at: 830 2064 2131 2749 4655 830 Size: 1234 67 618 1906 3478 Fragments arranged by size: 3478 1906 1234 618 67 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7303 PvuII CAG'CTG Cuts at: 2026 2026 Size: 7303 SacI G_AGCT'C Cuts at: 2256 2256 Size: 7303 SalI G'TCGA_C Cuts at: 1936 4645 1936 Size: 2709 4594 SbfI CC_TGCA'GG Cuts at: 4655 4655 Size: 7303 ScaI AGT'ACT Cuts at: 5551 7263 5551 Size: 1712 5591 SfcI C'TryA_G Cuts at: 826 1045 2060 2127 2745 3186 4417 4423 4651 Size: 219 1015 67 618 441 1231 6 228 Cuts at: 4651 5786 6467 6658 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1231 1135 1015 681 618 441 228 219 191 67 6 SfoI GGC'GCC Cuts at: 2078 2078 Size: 7303 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7303 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7303 SmaI CCC'GGG Cuts at: 1872 2074 4630 1872 Size: 202 2556 4545 Fragments arranged by size: 4545 2556 202 SmlI C'TyrA_G Cuts at: 820 1017 1943 5407 6308 6570 820 Size: 197 926 3464 901 262 1553 Fragments arranged by size: 3464 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 5094 493 Size: 4601 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7303 SphI G_CATG'C Cuts at: 1132 2477 4661 1132 Size: 1345 2184 3774 Fragments arranged by size: 3774 2184 1345 SspI AAT'ATT Cuts at: 5 52 3364 4235 5227 5 Size: 47 3312 871 992 2081 Fragments arranged by size: 3312 2081 992 871 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7303 StyI C'CwwG_G Cuts at: 513 1065 3060 3453 3723 4299 4332 4742 513 Size: 552 1995 393 270 576 33 410 3074 Fragments arranged by size: 3074 1995 576 552 410 393 270 33 SwaI ATTT'AAAT Cuts at: 2446 2446 Size: 7303 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3169 3736 4568 5330 5347 5500 5685 Size: 1255 1589 567 832 762 17 153 185 Cuts at: 5685 6130 325 Size: 445 1498 Fragments arranged by size: 1589 1498 1255 832 762 567 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3321 3516 4462 Size: 274 80 33 51 1443 1344 195 946 Cuts at: 4462 5549 7261 96 Size: 1087 1712 138 Fragments arranged by size: 1712 1443 1344 1087 946 274 195 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2617 2649 3112 3144 3431 3463 4473 4505 7263 Size: 32 463 32 287 32 1010 32 2758 Cuts at: 7263 7295 2617 Size: 32 2625 Fragments arranged by size: 2758 2625 1010 463 287 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7303 XbaI T'CTAG_A Cuts at: 3028 4639 3028 Size: 1611 5692 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3652 3652 Size: 7303 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7303 XmaI C'CCGG_G Cuts at: 1870 2072 4628 1870 Size: 202 2556 4545 Fragments arranged by size: 4545 2556 202 XmnI GAAnn'nnTTC Cuts at: 2508 3069 4172 5432 2508 Size: 561 1103 1260 4379 Fragments arranged by size: 4379 1260 1103 561 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5111 276 Size: 53 83 186 936 3577 2468 Fragments arranged by size: 3577 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI