(Circular) (Six-base) MAPSORT of: pFN21AE2508.seq Check: 8893 from: 1 to: 6067 pFN21AE2508 6067 bp With 182 enzymes: SgfI * October 25, 2019 12:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3877 278 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2276 3410 1937 Size: 339 1134 4594 Fragments arranged by size: 4594 1134 339 Acc65I G'GTAC_C Cuts at: 3388 3388 Size: 6067 AclI AA'CG_TT Cuts at: 1563 4194 4567 1563 Size: 2631 373 3063 Fragments arranged by size: 3063 2631 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2371 3024 4130 5514 5782 1632 Size: 739 653 1106 1384 268 1917 Fragments arranged by size: 1917 1384 1106 739 653 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5870 AflIII A'CryG_T Cuts at: 1203 4966 5943 1203 Size: 3763 977 1327 Fragments arranged by size: 3763 1327 977 AgeI A'CCGG_T Cuts at: 4898 4898 Size: 6067 AhdI GACnn_n'nnGTC Cuts at: 4796 4796 Size: 6067 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6067 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6067 AlwNI CAG_nnn'CTG Cuts at: 3224 3810 5382 3224 Size: 586 1572 3909 Fragments arranged by size: 3909 1572 586 ApaLI G'TGCA_C Cuts at: 4124 5280 4124 Size: 1156 4911 ApoI r'AATT_y Cuts at: 1493 3376 3602 3754 5863 1493 Size: 1883 226 152 2109 1697 Fragments arranged by size: 2109 1883 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2333 2405 4621 160 Size: 872 1301 72 2216 1606 Fragments arranged by size: 2216 1606 1301 872 72 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 AvaI C'yCGr_G Cuts at: 1870 1943 2209 3392 1870 Size: 73 266 1183 4545 Fragments arranged by size: 4545 1183 266 73 BamHI G'GATC_C Cuts at: 3397 3764 3397 Size: 367 5700 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3388 4843 618 Size: 325 217 627 1601 1455 1842 Fragments arranged by size: 1842 1601 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3246 3386 1391 Size: 1855 140 4072 Fragments arranged by size: 4072 1855 140 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2037 2841 3012 3065 928 Size: 971 138 804 171 53 3930 Fragments arranged by size: 3930 971 804 171 138 53 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4258 4292 1903 Size: 34 2321 34 3678 Fragments arranged by size: 3678 2321 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2488 3959 5169 2488 Size: 1471 1210 3386 Fragments arranged by size: 3386 1471 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6067 BfrBI ATG'CAT Cuts at: 2287 2770 2287 Size: 483 5584 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4678 136 Size: 107 122 71 631 605 3006 1525 Fragments arranged by size: 3006 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6062 6062 Size: 6067 BlpI GC'TnA_GC Cuts at: 3484 3484 Size: 6067 Bme1580I G_kGCm'C Cuts at: 1790 4128 5284 1790 Size: 2338 1156 2573 Fragments arranged by size: 2573 2338 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6067 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2206 3338 3798 Size: 416 53 83 399 24 789 1132 460 Cuts at: 3798 4756 442 Size: 958 1753 Fragments arranged by size: 1753 1132 958 789 460 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6067 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4727 1239 Size: 3488 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4192 5057 5355 4192 Size: 865 298 4904 Fragments arranged by size: 4904 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2919 4730 882 Size: 617 1420 1811 2219 Fragments arranged by size: 2219 1811 1420 617 BsaAI yAC'GTr Cuts at: 493 1204 3858 493 Size: 711 2654 2702 Fragments arranged by size: 2702 2654 711 BsaBI GATnn'nnATC Cuts at: 6061 6061 Size: 6067 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3874 4256 4891 275 Size: 53 83 186 936 2341 382 635 1451 Fragments arranged by size: 2341 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2418 4500 4898 5172 5319 5730 2418 Size: 2082 398 274 147 411 2755 Fragments arranged by size: 2755 2082 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3302 3332 1178 Size: 30 165 30 1899 30 3913 Fragments arranged by size: 3913 1899 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6067 BseYI C'CCAG_C Cuts at: 5270 5766 5270 Size: 496 5571 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3326 1163 Size: 573 1590 3904 Fragments arranged by size: 3904 1590 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3517 4278 5306 1540 Size: 275 186 1516 761 1028 2301 Fragments arranged by size: 2301 1516 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4128 4213 5284 4128 Size: 85 1071 4911 Fragments arranged by size: 4911 1071 85 BsmI GAATG_Cn' Cuts at: 3578 3671 3578 Size: 93 5974 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3246 3386 4128 4213 5284 1391 Size: 399 1456 140 742 85 1071 2174 Fragments arranged by size: 2174 1456 1071 742 399 140 85 BspEI T'CCGG_A Cuts at: 5730 5730 Size: 6067 BspHI T'CATG_A Cuts at: 3049 3956 5686 3049 Size: 907 1730 3430 Fragments arranged by size: 3430 1730 907 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3422 844 Size: 2578 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2701 3954 1394 Size: 429 878 1253 3507 Fragments arranged by size: 3507 1253 878 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2749 2998 4562 4736 65 Size: 1183 1501 249 1564 174 1396 Fragments arranged by size: 1564 1501 1396 1183 249 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4711 4898 1700 Size: 110 2901 187 2869 Fragments arranged by size: 2901 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2007 3255 96 Size: 1911 1248 2908 Fragments arranged by size: 2908 1911 1248 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6067 BssSI C'ACGA_G Cuts at: 4127 5139 4127 Size: 1012 5055 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6067 BstBI TT'CG_AA Cuts at: 4871 4871 Size: 6067 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6067 BstYI r'GATC_y Cuts at: 1972 3397 3764 4149 4166 5618 5704 5716 6062 Size: 1425 367 385 17 1452 86 12 346 Cuts at: 6062 1972 Size: 1977 Fragments arranged by size: 1977 1452 1425 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5515 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5328 Fragments arranged by size: 5328 683 56 BtsI GCAGTG_nn' Cuts at: 2111 3583 4377 4397 2111 Size: 1472 794 20 3781 Fragments arranged by size: 3781 1472 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6067 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6032 DraI TTT'AAA Cuts at: 3371 3371 Size: 6067 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5074 809 Size: 507 3758 1802 Fragments arranged by size: 3758 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3514 4403 6032 Size: 54 1425 50 275 18 1684 889 1629 Cuts at: 6032 8 Size: 43 Fragments arranged by size: 1684 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3514 1537 Size: 275 1702 4090 Fragments arranged by size: 4090 1702 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3997 1353 Size: 2644 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4654 5028 5174 387 Size: 1064 138 3065 374 146 1280 Fragments arranged by size: 3065 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2371 3024 4130 4727 5514 5782 1239 Size: 393 739 653 1106 597 787 268 1524 Fragments arranged by size: 1524 1106 787 739 653 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2253 2253 Size: 6067 EcoRI G'AATT_C Cuts at: 3376 3754 3376 Size: 378 5689 EcoRV GAT'ATC Cuts at: 2206 2206 Size: 6067 FspI TGC'GCA Cuts at: 4573 4573 Size: 6067 HaeII r_GCGC'y Cuts at: 1678 5214 1678 Size: 3536 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4708 4740 4782 4814 1373 Size: 32 135 32 3136 32 42 32 2626 Fragments arranged by size: 3136 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2946 3411 3657 669 Size: 988 281 1008 465 246 3079 Fragments arranged by size: 3079 1008 988 465 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6067 HpaI GTT'AAC Cuts at: 3657 3657 Size: 6067 KpnI G_GTAC'C Cuts at: 3392 3392 Size: 6067 MfeI C'AATT_G Cuts at: 3666 3666 Size: 6067 MluI A'CGCG_T Cuts at: 5943 5943 Size: 6067 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3052 5181 5365 1305 Size: 1747 2129 184 2007 Fragments arranged by size: 2129 2007 1747 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4245 Fragments arranged by size: 4245 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2352 2775 3051 4025 4384 4543 518 Size: 648 1186 423 276 974 359 159 2042 Fragments arranged by size: 2042 1186 974 648 423 359 276 159 MspA1I CmG'CkG Cuts at: 1606 2855 2911 3483 4160 5308 5553 1606 Size: 1249 56 572 677 1148 245 2120 Fragments arranged by size: 2120 1249 1148 677 572 245 56 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5515 NdeI CA'TA_TG Cuts at: 387 387 Size: 6067 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6067 NotI GC'GGCC_GC Cuts at: 3514 3514 Size: 6067 NsiI A_TGCA'T Cuts at: 2289 2772 2289 Size: 483 5584 NspI r_CATG'y Cuts at: 1132 1229 3425 4970 1132 Size: 97 2196 1545 2229 Fragments arranged by size: 2229 2196 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6067 PciI A'CATG_T Cuts at: 4966 4966 Size: 6067 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6067 PfoI T'CCnGG_A Cuts at: 3138 3138 Size: 6067 PmeI GTTT'AAAC Cuts at: 3371 3371 Size: 6067 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4136 4168 5649 5681 4136 Size: 32 1481 32 4522 Fragments arranged by size: 4522 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2253 2253 Size: 6067 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6067 PsiI TTA'TAA Cuts at: 3637 3637 Size: 6067 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3264 3296 3264 Size: 32 6035 PstI C_TGCA'G Cuts at: 830 3419 830 Size: 2589 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6067 PvuII CAG'CTG Cuts at: 2855 2911 2855 Size: 56 6011 SalI G'TCGA_C Cuts at: 1936 3409 1936 Size: 1473 4594 SbfI CC_TGCA'GG Cuts at: 3419 3419 Size: 6067 ScaI AGT'ACT Cuts at: 4315 6027 4315 Size: 1712 4355 SfcI C'TryA_G Cuts at: 826 1045 2142 2526 3174 3415 4550 5231 5422 Size: 219 1097 384 648 241 1135 681 191 Cuts at: 5422 826 Size: 1471 Fragments arranged by size: 1471 1135 1097 681 648 384 241 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6067 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6067 SmaI CCC'GGG Cuts at: 1872 3394 1872 Size: 1522 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2209 4171 5072 5334 820 Size: 197 926 266 1962 901 262 1553 Fragments arranged by size: 1962 1553 926 901 266 262 197 SnaBI TAC'GTA Cuts at: 493 3858 493 Size: 3365 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6067 SphI G_CATG'C Cuts at: 1132 3425 1132 Size: 2293 3774 SspI AAT'ATT Cuts at: 5 52 2343 2518 2673 3014 3991 5 Size: 47 2291 175 155 341 977 2081 Fragments arranged by size: 2291 2081 977 341 175 155 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6067 StyI C'CwwG_G Cuts at: 513 1065 3506 513 Size: 552 2441 3074 Fragments arranged by size: 3074 2441 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4094 4111 4264 4449 4894 325 Size: 1255 2514 17 153 185 445 1498 Fragments arranged by size: 2514 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2007 3255 4313 Size: 274 80 33 51 1443 30 1248 1058 Cuts at: 4313 6025 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1248 1058 274 138 80 51 33 30 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3302 3334 6027 6059 3302 Size: 32 2693 32 3310 Fragments arranged by size: 3310 2693 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6067 XbaI T'CTAG_A Cuts at: 3403 3403 Size: 6067 XhoI C'TCGA_G Cuts at: 1943 2209 1943 Size: 266 5801 XmaI C'CCGG_G Cuts at: 1870 3392 1870 Size: 1522 4545 XmnI GAAnn'nnTTC Cuts at: 2232 4196 2232 Size: 1964 4103 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3875 276 Size: 53 83 186 936 2341 2468 Fragments arranged by size: 2468 2341 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI