Restriction map for
pFN21AE3069
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# NO CUT ENZYMES ( 179 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AgeI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI Asp700I Asp718I
AspHI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfaI BfrI BglI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsiYI BslI BsmBI BsoBI Bsp1286I
Bsp1407I BspEI BspHI BspMI BsrDI
BsrFI BsrGI BsrI BssHII Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstYI BstZ17I Bsu36I CelII
Cfr10I Cfr13I CpoI DraI DraII
DraIII EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoT22I FokI FseI FspI
HapII HgaI Hin1I HpaI HpaII
KasI KpnI Ksp632I KspI MaeI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AflIII 86 ACATGT A^C[GA][CT]GT
AhdI 577 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 532 ATTAAT AT^TAAT
AsnI 532 ATTAAT AT^TAAT
AspEI 577 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BbsI 442 GAAGAC GAAGAC(2/6)
BclI 69 TGATCA T^GATCA
BpuAI 442 GAAGAC GAAGAC(2/6)
BsmI 172 GCATTC GAATGC(1/-1)
BspDI 193 ATCGAT AT^CGAT
BspLU11I 86 ACATGT A^CATGT
BsrBI 448 GAGCGG CCGCTC(-3/-3)
BssSI 633 CACGAG CACGAG(-5/-1)
BstXI 17 CCATGCAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 193 ATCGAT AT^CGAT
DdeI 464 CTGAG C^T[ACGT]AG
DrdI 445 GACGAGCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 308 CCATGG C^C[GA][CT]GG
EaeI 51 CGGCCA [CT]^GGCC[GA]
Eam1105I 577 GACAAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 565 GATATC GAT^ATC
FauI 450 GCGGG CCCGC(4/6)
FbaI 69 TGATCA T^GATCA
HindIII 238 AAGCTT A^AGCTT
HphI 284 TCACC GGTGA(8/7)
MaeIII 434 GTGAC ^GT[ACGT]AC
MslI 18 CATGCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 308 CCATGG C^CATGG
NlaIV 304 GGAGCC GG[ACGT]^[ACGT]CC
NspI 86 ACATGT [GA]CATG^[CT]
PshBI 532 ATTAAT AT^TAAT
SfaNI 40 GATGC GCATC(5/9)
SmlI 640 CTCAAG C^T[CT][GA]AG
TaqI 194 TCGA T^CGA
Tsp45I 434 GTGAC ^GT[GC]AC
Tsp509I 95 AATT ^AATT
TthHB8I 194 TCGA T^CGA
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# TWO CUT ENZYMES ( 31 enzymes) #
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AciI 661 CCGC CCGC(-3/-1)
450 GCGG
AfaI 48 GTAC GT^AC
361 GTAC
AluI 239 AGCT AG^CT
491 AGCT
AlwI 604 GATCC GGATC(4/5)
191 GGATC
BbvI 663 GCAGC GCAGC(8/12)
619 GCTGC
BsaJI 308 CCATGG C^C[ACGT][ACGT]GG
521 CCAAGG
BsmFI 251 GGGAC GGGAC(10/14)
398 GGGAC
BssKI 44 CCAGG ^CC[ACGT]GG
264 CCAGG
BstNI 44 CCAGG CC^[AT]GG
264 CCAGG
Cac8I 259 GCAGGC GC[ACGT]^[ACGT]GC
676 GCCTGC
CfoI 13 GCGC GCG^C
555 GCGC
EcoRII 44 CCAGG ^CC[AT]GG
264 CCAGG
EcoT14I 308 CCATGG C^C[AT][AT]GG
521 CCAAGG
Fnu4HI 619 GCTGC GC^[ACGT]GC
663 GCAGC
HaeII 12 AGCGCC [GA]GCGC^[CT]
554 AGCGCC
HhaI 13 GCGC GCG^C
555 GCGC
HinP1I 13 GCGC G^CGC
555 GCGC
HincII 372 GTCAAC GT[CT]^[GA]AC
473 GTCAAC
HindII 372 GTCAAC GT[CT]^[GA]AC
473 GTCAAC
ItaI 619 GCTGC GC^[ACGT]GC
663 GCAGC
MboII 343 GAAGA GAAGA(8/7)
442 GAAGA
MseI 322 TTAA T^TAA
533 TTAA
MvaI 44 CCAGG CC^[AT]GG
264 CCAGG
MwoI 34 GCCCAAGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
610 GCCATCACTGC
RsaI 48 GTAC GT^AC
361 GTAC
ScrFI 44 CCAGG CC^[ACGT]GG
264 CCAGG
StyI 308 CCATGG C^C[AT][AT]GG
521 CCAAGG
TfiI 233 GAATC G^A[AT]TC
346 GAATC
Tru9I 322 TTAA T^TAA
533 TTAA
TseI 619 GCTGC G^C[AT]GC
663 GCAGC
TspRI 117 CAGTG CA[GC]TG[ACGT][ACGT]^
615 CACTG
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# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
BseRI BsmAI DpnII HaeIII HinfI
MboI MnlI NdeII NlaIII PleI
Sau3AI