################################## # NO CUT ENZYMES ( 100 enzymes) # ################################## AatII Acc65I AccI AclI AflII AgeI AhdI Alw44I ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BamHI BbrPI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BseRI BsgI BsiWI BsmI Bsp1407I BspDI BspHI BspMI BsrGI Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI DrdI Eam1105I EcoRI EcoRV EcoT22I FbaI FseI FspI HindIII HpaI KpnI MfeI MluI MseI MunI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SalI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccIII 1075 TCCGGA T^CCGGA AcsI 1236 GAATTT [GA]^AATT[CT] AflIII 557 ACATGT A^C[GA][CT]GT AlwI 801 GATCC GGATC(4/5) AlwNI 265 CAGCCGCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1399 CCTAAGG CC^T[ACGT]AGG ApoI 1236 GAATTT [GA]^AATT[CT] Asp700I 947 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BbsI 1276 GAAGAC GAAGAC(2/6) BfaI 970 CTAG C^TAG BpuAI 1276 GAAGAC GAAGAC(2/6) BsaWI 1075 TCCGGA [AT]^CCGG[AT] BseAI 1075 TCCGGA T^CCGGA BsmBI 151 GAGACG CGTCTC(1/5) BsmFI 1059 GTCCC GGGAC(10/14) BspEI 1075 TCCGGA T^CCGGA BspLU11I 557 ACATGT A^CATGT BsrDI 822 CATTGC GCAATG(2/0) BsrFI 44 ACCGGC [GA]^CCGG[CT] BssSI 862 CTCGTG CACGAG(-5/-1) BstEII 222 GGTGACC G^GT[ACGT]ACC BstPI 222 GGTGACC G^GT[ACGT]ACC BstYI 800 AGATCC [GA]^GATC[CT] Bsu36I 1399 CCTAAGG CC^T[ACGT]AGG Cfr10I 44 ACCGGC [GA]^CCGG[CT] DraII 1057 GGGTCCC [GA]G^G[ACGT]CC[CT] DsaI 692 CCGCGG C^C[GA][CT]GG Eco81I 1399 CCTAAGG CC^T[ACGT]AGG EcoO109I 1057 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO65I 222 GGTGACC G^GT[ACGT]ACC EcoT14I 1224 CCTTGG C^C[AT][AT]GG HgaI 433 GCGTC GACGC(5/10) HincII 880 GTCAAC GT[CT]^[GA]AC HindII 880 GTCAAC GT[CT]^[GA]AC KspI 692 CCGCGG CCGC^GG MaeI 970 CTAG C^TAG MflI 800 AGATCC [GA]^GATC[CT] MroI 1075 TCCGGA T^CCGGA PflMI 384 CCACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1057 GGGTCCC [GA]G^G[AT]CC[CT] PstI 487 CTGCAG CTGCA^G SacI 1407 GAGCTC GAGCT^C SacII 692 CCGCGG CCGC^GG SapI 297 GCTCTTC GCTCTTC(1/4) SmlI 33 CTTGAG C^T[CT][GA]AG SphI 635 GCATGC GCATG^C StyI 1224 CCTTGG C^C[AT][AT]GG Van91I 384 CCACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 800 AGATCC [GA]^GATC[CT] XmnI 947 GAATTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AcyI 764 GGCGCC G[GA]^CG[CT]C 793 GGCGCC Aor51HI 347 AGCGCT AGC^GCT 1070 AGCGCT AvaII 521 GGACC G^G[AT]CC 1058 GGTCC BalI 110 TGGCCA TGG^CCA 356 TGGCCA BbeI 764 GGCGCC GGCGC^C 793 GGCGCC BbvCI 318 CCTCAGC CCTCAGC(-5/-2) 750 CCTCAGC BsaAI 234 CACGTA [CT]AC^GT[GA] 388 TACGTG BsaHI 764 GGCGCC G[GA]^CG[CT]C 793 GGCGCC BsaI 1312 GAGACC GGTCTC(1/5) 469 GGTCTC BsrBI 859 CCGCTC CCGCTC(-3/-3) 1377 GAGCGG BssHII 452 GCGCGC G^CGCGC 623 GCGCGC BstAPI 323 GCATCATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 424 GCAGTCTATGC BstXI 496 CCAGACACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 827 CCACCTCCCTGG DraIII 385 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG 501 CACCTGGTG EagI 689 CGGCCG C^GGCCG 695 CGGCCG EclXI 689 CGGCCG C^GGCCG 695 CGGCCG Eco47III 347 AGCGCT AGC^GCT 1070 AGCGCT Eco52I 689 CGGCCG C^GGCCG 695 CGGCCG EcoNI 1266 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1286 CCTCACTGAGG Hin1I 764 GGCGCC G[GA]^CG[CT]C 793 GGCGCC HinfI 1321 GACTC G^A[ACGT]TC 1339 GACTC KasI 764 GGCGCC G^GCGCC 793 GGCGCC MluNI 110 TGGCCA TGG^CCA 356 TGGCCA MscI 110 TGGCCA TGG^CCA 356 TGGCCA NarI 764 GGCGCC GG^CGCC 793 GGCGCC NotI 688 GCGGCCGC GC^GGCCGC 694 GCGGCCGC NspI 557 ACATGT [GA]CATG^[CT] 635 GCATGC PleI 1321 GACTC GAGTC(4/5) 1339 GACTC SexAI 104 ACCTGGT A^CC[AT]GGT 502 ACCTGGT SfaNI 323 GCATC GCATC(5/9) 785 GCATC SfcI 378 CTACAG C^T[GA][CT]AG 487 CTGCAG SfoI 764 GGCGCC GGC^GCC 793 GGCGCC TaqI 344 TCGA T^CGA 515 TCGA TthHB8I 344 TCGA T^CGA 515 TCGA XcmI 827 CCACCTCCCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 854 CCATCCCGCTCGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AccII AciI AfaI AluI AspHI AvaI BanI BanII BbvI BcnI BglI BmyI BsaBI BsaJI BsiEI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HpaII HphI ItaI Ksp632I MaeII MaeIII MamI MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SmaI TseI Tsp45I Tsp509I TspRI XmaCI XmaI