Restriction map for
pFN21AE9419
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BanII BbrPI BbsI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaI BsgI BsiWI BsmI
Bsp1407I BspDI BspHI BsrBI BsrDI
BsrFI BsrGI BstBI BstXI Bsu36I
CelII Cfr10I ClaI CpoI DdeI
DraI DraIII DrdI Eam1105I Eco47III
Eco81I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MfeI MluNI MscI
MseI MunI NaeI NdeI NgoMIV
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI ScaI SexAI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp509I Tth111I
XbaI XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 483 GACGTC GACGT^C
AccIII 135 TCCGGA T^CCGGA
AscI 175 GGCGCGCC GG^CGCGCC
BbeI 1302 GGCGCC GGCGC^C
BglI 673 GCCGCGCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 243 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 135 TCCGGA T^CCGGA
BseRI 730 GAGGAG GAGGAG(10/8)
BsmBI 1223 CGTCTC CGTCTC(1/5)
BspEI 135 TCCGGA T^CCGGA
BspLU11I 494 ACATGT A^CATGT
BssHII 176 GCGCGC G^CGCGC
Bst1107I 1102 GTATAC GTA^TAC
BstAPI 807 GCAGAAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 969 GGTCACC G^GT[ACGT]ACC
BstPI 969 GGTCACC G^GT[ACGT]ACC
BstYI 659 GGATCT [GA]^GATC[CT]
BstZ17I 1102 GTATAC GTA^TAC
DraII 1295 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO109I 1295 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 969 GGTCACC G^GT[ACGT]ACC
HinfI 243 GATTC G^A[ACGT]TC
KasI 1302 GGCGCC G^GCGCC
MaeII 484 ACGT A^CGT
MamI 243 GATTCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 659 GGATCT [GA]^GATC[CT]
MroI 135 TCCGGA T^CCGGA
NarI 1302 GGCGCC GG^CGCC
NcoI 476 CCATGG C^CATGG
NheI 60 GCTAGC G^CTAGC
NotI 1315 GCGGCCGC GC^GGCCGC
PflMI 543 CCATTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SfiI 672 GGCCGCGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1302 GGCGCC GGC^GCC
SmlI 1198 CTCAAG C^T[CT][GA]AG
TfiI 243 GATTC G^A[AT]TC
Van91I 543 CCATTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 659 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1102 GTATAC GT^[AC][GT]AC
1360 GTCTAC
AfaI 261 GTAC GT^AC
498 GTAC
AlwI 659 GGATC GGATC(4/5)
1281 GGATC
AlwNI 624 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1285 CAGGGGCTG
AspHI 394 GTGCTC G[AT]GC[AT]^C
1273 GAGCAC
BanI 1068 GGTGCC G^G[CT][GA]CC
1302 GGCGCC
BfaI 61 CTAG C^TAG
1129 CTAG
BsiHKAI 394 GTGCTC G[AT]GC[AT]^C
1273 GAGCAC
BsmAI 957 GAGAC GTCTC(1/5)
1224 GTCTC
BsrI 263 ACTGG ACTGG(1/-1)
692 CCAGT
BstF5I 190 CATCC GGATG(2/0)
1047 CATCC
EcoNI 90 CCTTCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1092 CCTGTGCCAGG
EcoT14I 476 CCATGG C^C[AT][AT]GG
641 CCAAGG
FokI 190 CATCC GGATG(9/13)
1047 CATCC
KspI 917 CCGCGG CCGC^GG
1319 CCGCGG
MaeI 61 CTAG C^TAG
1129 CTAG
MluI 119 ACGCGT A^CGCGT
1106 ACGCGT
PstI 127 CTGCAG CTGCA^G
1291 CTGCAG
PvuII 624 CAGCTG CAG^CTG
1143 CAGCTG
RsaI 261 GTAC GT^AC
498 GTAC
SacII 917 CCGCGG CCGC^GG
1319 CCGCGG
SapI 318 GAAGAGC GCTCTTC(1/4)
564 GAAGAGC
SmaI 195 CCCGGG CCC^GGG
233 CCCGGG
SphI 323 GCATGC GCATG^C
1241 GCATGC
StyI 476 CCATGG C^C[AT][AT]GG
641 CCAAGG
TaqI 964 TCGA T^CGA
1231 TCGA
TthHB8I 964 TCGA T^CGA
1231 TCGA
XmaCI 195 CCCGGG C^CCGGG
233 CCCGGG
XmaI 195 CCCGGG C^CCGGG
233 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AccII AciI AcyI AflIII AluI
AvaI AvaII BbvI BcnI BmyI
BpmI BsaHI BsaJI BsaWI BsiEI
BsiYI BslI BsmFI BsoBI Bsp1286I
BspMI BssKI BssSI BstNI BstUI
Cac8I CfoI Cfr13I DpnII DsaI
EaeI EagI EarI EclXI Eco52I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI Sau3AI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI