(Circular) (Six-base) MAPSORT of: pFN21AE9419.seq Check: 7108 from: 1 to: 6112 pFN21AE9419 6112 bp With 182 enzymes: SgfI * September 21, 2017 12:07 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2492 3922 278 Size: 53 83 186 936 956 1430 2468 Fragments arranged by size: 2468 1430 956 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3108 3366 3455 1937 Size: 1171 258 89 4594 Fragments arranged by size: 4594 1171 258 89 Acc65I G'GTAC_C Cuts at: 3433 3433 Size: 6112 AclI AA'CG_TT Cuts at: 1563 4239 4612 1563 Size: 2676 373 3063 Fragments arranged by size: 3063 2676 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2062 2588 3064 4175 5559 5827 1632 Size: 430 526 476 1111 1384 268 1917 Fragments arranged by size: 1917 1384 1111 526 476 430 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5915 AflIII A'CryG_T Cuts at: 1203 2124 2499 3111 5011 5988 1203 Size: 921 375 612 1900 977 1327 Fragments arranged by size: 1900 1327 977 921 612 375 AgeI A'CCGG_T Cuts at: 4943 4943 Size: 6112 AhdI GACnn_n'nnGTC Cuts at: 4841 4841 Size: 6112 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6112 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6112 AlwNI CAG_nnn'CTG Cuts at: 2634 3295 3855 5427 2634 Size: 661 560 1572 3319 Fragments arranged by size: 3319 1572 661 560 ApaLI G'TGCA_C Cuts at: 4169 5325 4169 Size: 1156 4956 ApoI r'AATT_y Cuts at: 1493 3421 3647 3799 5908 1493 Size: 1928 226 152 2109 1697 Fragments arranged by size: 2109 1928 1697 226 152 AscI GG'CGCG_CC Cuts at: 2181 2181 Size: 6112 AseI AT'TA_AT Cuts at: 160 1032 4666 160 Size: 872 3634 1606 Fragments arranged by size: 3634 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 AvaI C'yCGr_G Cuts at: 1870 1943 2200 2238 2583 3437 1870 Size: 73 257 38 345 854 4545 Fragments arranged by size: 4545 854 345 257 73 38 BamHI G'GATC_C Cuts at: 3442 3809 3442 Size: 367 5745 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3073 3307 3433 4888 618 Size: 325 217 627 1286 234 126 1455 1842 Fragments arranged by size: 1842 1455 1286 627 325 234 217 126 BanII G_rGCy'C Cuts at: 1391 3431 1391 Size: 2040 4072 BbeI G_GCGC'C Cuts at: 3311 3311 Size: 6112 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5141 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4303 4337 1903 Size: 34 50 34 2282 34 3678 Fragments arranged by size: 3678 2282 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4004 5214 4004 Size: 1210 4902 BclI T'GATC_A Cuts at: 1548 1548 Size: 6112 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2684 4723 136 Size: 107 122 71 631 605 1012 2039 1525 Fragments arranged by size: 2039 1525 1012 631 605 122 107 71 BglII A'GATC_T Cuts at: 6107 6107 Size: 6112 BlpI GC'TnA_GC Cuts at: 3529 3529 Size: 6112 Bme1580I G_kGCm'C Cuts at: 1790 3078 4173 5329 1790 Size: 1288 1095 1156 2573 Fragments arranged by size: 2573 1288 1156 1095 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6112 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2278 2691 3843 Size: 416 53 83 399 24 861 413 1152 Cuts at: 3843 4801 442 Size: 958 1753 Fragments arranged by size: 1753 1152 958 861 416 413 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2069 1056 Size: 1013 5099 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2120 2186 3067 4772 1239 Size: 881 66 881 1705 2579 Fragments arranged by size: 2579 1705 881 881 66 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3188 4237 5102 5400 3188 Size: 1049 865 298 3900 Fragments arranged by size: 3900 1049 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4775 882 Size: 617 3276 2219 Fragments arranged by size: 3276 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3903 493 Size: 711 2699 2702 Fragments arranged by size: 2702 2699 711 BsaBI GATnn'nnATC Cuts at: 2252 6106 2252 Size: 3854 2258 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2489 2938 3308 3357 Size: 53 83 186 936 956 449 370 49 Cuts at: 3357 3919 4301 4936 275 Size: 562 382 635 1451 Fragments arranged by size: 1451 956 936 635 562 449 382 370 186 83 53 49 BsaWI w'CCGG_w Cuts at: 2140 2577 2945 4545 4943 5217 5364 5775 2140 Size: 437 368 1600 398 274 147 411 2477 Fragments arranged by size: 2477 1600 437 411 398 368 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5887 Fragments arranged by size: 5887 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2750 1186 Size: 1564 4548 BseYI C'CCAG_C Cuts at: 2158 2770 2794 2824 2842 3395 5315 5811 2158 Size: 612 24 30 18 553 1920 496 2459 Fragments arranged by size: 2459 1920 612 553 496 30 24 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5539 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2596 2614 2893 2946 3324 3562 Size: 275 186 595 18 279 53 378 238 Cuts at: 3562 4323 5351 1540 Size: 761 1028 2301 Fragments arranged by size: 2301 1028 761 595 378 279 275 238 186 53 18 BsiHKAI G_wGCw'C Cuts at: 2403 3282 4173 4258 5329 2403 Size: 879 891 85 1071 3186 Fragments arranged by size: 3186 1071 891 879 85 BsmI GAATG_Cn' Cuts at: 3623 3716 3623 Size: 93 6019 BsmBI CGTCTCn'nnnn_ Cuts at: 3234 3234 Size: 6112 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2403 3078 3282 3431 4173 4258 5329 Size: 399 613 675 204 149 742 85 1071 Cuts at: 5329 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 675 613 399 204 149 85 BspEI T'CCGG_A Cuts at: 2140 5775 2140 Size: 3635 2477 BspHI T'CATG_A Cuts at: 4001 5731 4001 Size: 1730 4382 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2310 2874 3345 3396 3467 844 Size: 1466 564 471 51 71 3489 Fragments arranged by size: 3489 1466 564 471 71 51 BsrBI CCG'CTC Cuts at: 1394 1823 3999 1394 Size: 429 2176 3507 Fragments arranged by size: 3507 2176 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4607 4781 65 Size: 1183 3359 174 1396 Fragments arranged by size: 3359 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4756 4943 1700 Size: 110 2946 187 2869 Fragments arranged by size: 2946 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6112 BssHII G'CGCG_C Cuts at: 1925 2181 1925 Size: 256 5856 BssSI C'ACGA_G Cuts at: 2113 3176 3281 4172 5184 2113 Size: 1063 105 891 1012 3041 Fragments arranged by size: 3041 1063 1012 891 105 BstAPI GCAn_nnn'nTGC Cuts at: 794 2818 794 Size: 2024 4088 BstBI TT'CG_AA Cuts at: 4916 4916 Size: 6112 BstEII G'GTnAC_C Cuts at: 1184 2974 1184 Size: 1790 4322 BstXI CCAn_nnnn'nTGG Cuts at: 2011 2011 Size: 6112 BstYI r'GATC_y Cuts at: 1972 2664 3442 3809 4194 4211 5663 5749 5761 Size: 692 778 367 385 17 1452 86 12 Cuts at: 5761 6107 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 778 692 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3109 3109 Size: 6112 BtgI C'CryG_G Cuts at: 513 1065 2481 2715 2922 3324 513 Size: 552 1416 234 207 402 3301 Fragments arranged by size: 3301 1416 552 402 234 207 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2869 3208 487 Size: 683 56 1643 339 3391 Fragments arranged by size: 3391 1643 683 339 56 BtsI GCAGTG_nn' Cuts at: 2523 3628 4422 4442 2523 Size: 1105 794 20 4193 Fragments arranged by size: 4193 1105 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6112 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2098 2133 529 Size: 35 1534 35 4508 Fragments arranged by size: 4508 1534 35 35 DraI TTT'AAA Cuts at: 3416 3416 Size: 6112 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5119 809 Size: 507 3803 1802 Fragments arranged by size: 3803 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2013 2106 2593 Size: 54 1425 50 275 18 183 93 487 Cuts at: 2593 2611 2643 2676 3321 3559 4448 6077 8 Size: 18 32 33 645 238 889 1629 43 Fragments arranged by size: 1629 1425 889 645 487 275 238 183 93 54 50 43 33 32 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2593 2611 3321 3559 1537 Size: 275 781 18 710 238 4090 Fragments arranged by size: 4090 781 710 275 238 18 EarI CTCTTCn'nnn_ Cuts at: 1353 2081 2318 2366 2564 4042 1353 Size: 728 237 48 198 1478 3423 Fragments arranged by size: 3423 1478 728 237 198 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4699 5073 5219 387 Size: 1064 138 3110 374 146 1280 Fragments arranged by size: 3110 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2062 2120 2186 2588 3064 3067 4175 Size: 393 430 58 66 402 476 3 1108 Cuts at: 4175 4772 5559 5827 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1108 787 597 476 430 402 393 268 66 58 3 EcoNI CCTnn'n_nnAGG Cuts at: 2099 3101 2099 Size: 1002 5110 EcoO109I rG'GnC_Cy Cuts at: 3301 3301 Size: 6112 EcoRI G'AATT_C Cuts at: 3421 3799 3421 Size: 378 5734 FspI TGC'GCA Cuts at: 4618 4618 Size: 6112 HaeII r_GCGC'y Cuts at: 1678 2381 2654 2958 3038 3203 3311 3382 5259 Size: 703 273 304 80 165 108 71 1877 Cuts at: 5259 1678 Size: 2531 Fragments arranged by size: 2531 1877 703 304 273 165 108 80 71 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4753 4785 4827 4859 1373 Size: 32 135 32 3181 32 42 32 2626 Fragments arranged by size: 3181 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3456 3702 669 Size: 988 281 1518 246 3079 Fragments arranged by size: 3079 1518 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6112 HpaI GTT'AAC Cuts at: 3702 3702 Size: 6112 KasI G'GCGC_C Cuts at: 3307 3307 Size: 6112 KpnI G_GTAC'C Cuts at: 3437 3437 Size: 6112 MfeI C'AATT_G Cuts at: 3711 3711 Size: 6112 MluI A'CGCG_T Cuts at: 2124 3111 5988 2124 Size: 987 2877 2248 Fragments arranged by size: 2877 2248 987 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3168 5226 5410 1305 Size: 1863 2058 184 2007 Fragments arranged by size: 2058 2007 1863 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4290 Fragments arranged by size: 4290 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2258 2498 2528 2534 3245 4070 Size: 648 843 249 240 30 6 711 825 Cuts at: 4070 4429 4588 518 Size: 359 159 2042 Fragments arranged by size: 2042 843 825 711 648 359 249 240 159 30 6 MspA1I CmG'CkG Cuts at: 1606 2208 2631 2842 2924 3150 3326 3372 3528 Size: 602 423 211 82 226 176 46 156 Cuts at: 3528 4205 5353 5598 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 602 423 245 226 211 176 156 82 46 NarI GG'CG_CC Cuts at: 3308 3308 Size: 6112 NcoI C'CATG_G Cuts at: 513 1065 2481 513 Size: 552 1416 4144 Fragments arranged by size: 4144 1416 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6112 NheI G'CTAG_C Cuts at: 1052 2065 1052 Size: 1013 5099 NotI GC'GGCC_GC Cuts at: 3321 3559 3321 Size: 238 5874 NspI r_CATG'y Cuts at: 1132 1229 2332 2503 3250 3470 5015 1132 Size: 97 1103 171 747 220 1545 2229 Fragments arranged by size: 2229 1545 1103 747 220 171 97 PasI CC'CwG_GG Cuts at: 1348 2284 1348 Size: 936 5176 PciI A'CATG_T Cuts at: 2499 5011 2499 Size: 2512 3600 PflMI CCAn_nnn'nTGG Cuts at: 1108 2554 1108 Size: 1446 4666 PfoI T'CCnGG_A Cuts at: 2088 2757 2088 Size: 669 5443 PmeI GTTT'AAAC Cuts at: 3416 3416 Size: 6112 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4181 4213 5694 5726 4181 Size: 32 1481 32 4567 Fragments arranged by size: 4567 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6112 PsiI TTA'TAA Cuts at: 3682 3682 Size: 6112 PstI C_TGCA'G Cuts at: 830 2136 3300 3464 830 Size: 1306 1164 164 3478 Fragments arranged by size: 3478 1306 1164 164 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6112 PvuII CAG'CTG Cuts at: 2631 3150 2631 Size: 519 5593 SacII CC_GC'GG Cuts at: 2925 3327 2925 Size: 402 5710 SalI G'TCGA_C Cuts at: 1936 3454 1936 Size: 1518 4594 SapI GCTCTTCn'nnn_ Cuts at: 2318 2564 2318 Size: 246 5866 SbfI CC_TGCA'GG Cuts at: 3464 3464 Size: 6112 ScaI AGT'ACT Cuts at: 4360 6072 4360 Size: 1712 4400 SfcI C'TryA_G Cuts at: 826 1045 2132 3296 3367 3460 4595 5276 5467 Size: 219 1087 1164 71 93 1135 681 191 Cuts at: 5467 826 Size: 1471 Fragments arranged by size: 1471 1164 1135 1087 681 219 191 93 71 SfiI GGCCn_nnn'nGGCC Cuts at: 2684 2684 Size: 6112 SfoI GGC'GCC Cuts at: 3309 3309 Size: 6112 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6112 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6112 SmaI CCC'GGG Cuts at: 1872 2202 2240 3439 1872 Size: 330 38 1199 4545 Fragments arranged by size: 4545 1199 330 38 SmlI C'TyrA_G Cuts at: 820 1017 1943 3203 4216 5117 5379 820 Size: 197 926 1260 1013 901 262 1553 Fragments arranged by size: 1553 1260 1013 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3903 493 Size: 3410 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6112 SphI G_CATG'C Cuts at: 1132 2332 3250 3470 1132 Size: 1200 918 220 3774 Fragments arranged by size: 3774 1200 918 220 SspI AAT'ATT Cuts at: 5 52 4036 5 Size: 47 3984 2081 Fragments arranged by size: 3984 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6112 StyI C'CwwG_G Cuts at: 513 1065 2481 2646 3551 513 Size: 552 1416 165 905 3074 Fragments arranged by size: 3074 1416 905 552 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2260 4139 4156 4309 4494 4939 325 Size: 1255 680 1879 17 153 185 445 1498 Fragments arranged by size: 1879 1498 1255 680 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2265 4358 6070 Size: 274 80 33 51 1443 288 2093 1712 Cuts at: 6070 96 Size: 138 Fragments arranged by size: 2093 1712 1443 288 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2135 2167 6072 6104 2135 Size: 32 3905 32 2143 Fragments arranged by size: 3905 2143 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6112 XbaI T'CTAG_A Cuts at: 3448 3448 Size: 6112 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6112 XmaI C'CCGG_G Cuts at: 1870 2200 2238 3437 1870 Size: 330 38 1199 4545 Fragments arranged by size: 4545 1199 330 38 XmnI GAAnn'nnTTC Cuts at: 4241 4241 Size: 6112 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2490 3920 276 Size: 53 83 186 936 956 1430 2468 Fragments arranged by size: 2468 1430 956 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SrfI SwaI XcmI