Restriction map for
pFN21AE9419
(Linear) (Six-base) MAPSORT of: KIEE9419.seq Check: 2358 from: 1 to: 1407
KIEE9419.orf
With 182 enzymes: SgfI *
September 21, 2017 11:26 ..
AatII G_ACGT'C
Cuts at: 0 487 1407
Size: 487 920
AccI GT'mk_AC
Cuts at: 0 1103 1361 1407
Size: 1103 258 46
Fragments arranged by size:
1103 258 46
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 583 1059 1407
Size: 57 526 476 348
Fragments arranged by size:
526 476 348 57
AflIII A'CryG_T
Cuts at: 0 119 494 1106 1407
Size: 119 375 612 301
Fragments arranged by size:
612 375 301 119
AlwNI CAG_nnn'CTG
Cuts at: 0 629 1290 1407
Size: 629 661 117
Fragments arranged by size:
661 629 117
AscI GG'CGCG_CC
Cuts at: 0 176 1407
Size: 176 1231
AvaI C'yCGr_G
Cuts at: 0 195 233 578 1407
Size: 195 38 345 829
Fragments arranged by size:
829 345 195 38
BanI G'GyrC_C
Cuts at: 0 1068 1302 1407
Size: 1068 234 105
Fragments arranged by size:
1068 234 105
BbeI G_GCGC'C
Cuts at: 0 1306 1407
Size: 1306 101
BglI GCCn_nnn'nGGC
Cuts at: 0 679 1407
Size: 679 728
Bme1580I G_kGCm'C
Cuts at: 0 1073 1407
Size: 1073 334
BmrI ACTGGGnnnn_n'
Cuts at: 0 273 686 1407
Size: 273 413 721
Fragments arranged by size:
721 413 273
BmtI G_CTAG'C
Cuts at: 0 64 1407
Size: 64 1343
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 181 1062 1407
Size: 115 66 881 345
Fragments arranged by size:
881 345 115 66
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1183 1407
Size: 1183 224
BsaBI GATnn'nnATC
Cuts at: 0 247 1407
Size: 247 1160
BsaHI Gr'CG_yC
Cuts at: 0 484 933 1303 1352 1407
Size: 484 449 370 49 55
Fragments arranged by size:
484 449 370 55 49
BsaWI w'CCGG_w
Cuts at: 0 135 572 940 1407
Size: 135 437 368 467
Fragments arranged by size:
467 437 368 135
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 745 1407
Size: 745 662
BseYI C'CCAG_C
Cuts at: 0 153 765 789 819 837 1390 1407
Size: 153 612 24 30 18 553 17
Fragments arranged by size:
612 553 153 30 24 18 17
BsiEI CG_ry'CG
Cuts at: 0 591 609 888 941 1319 1407
Size: 591 18 279 53 378 88
Fragments arranged by size:
591 378 279 88 53 18
BsiHKAI G_wGCw'C
Cuts at: 0 398 1277 1407
Size: 398 879 130
Fragments arranged by size:
879 398 130
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1229 1407
Size: 1229 178
Bsp1286I G_dGCh'C
Cuts at: 0 398 1073 1277 1407
Size: 398 675 204 130
Fragments arranged by size:
675 398 204 130
BspEI T'CCGG_A
Cuts at: 0 135 1407
Size: 135 1272
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 305 869 1340 1391 1407
Size: 305 564 471 51 16
Fragments arranged by size:
564 471 305 51 16
BssHII G'CGCG_C
Cuts at: 0 176 1407
Size: 176 1231
BssSI C'ACGA_G
Cuts at: 0 108 1171 1276 1407
Size: 108 1063 105 131
Fragments arranged by size:
1063 131 108 105
BstAPI GCAn_nnn'nTGC
Cuts at: 0 813 1407
Size: 813 594
BstEII G'GTnAC_C
Cuts at: 0 969 1407
Size: 969 438
BstYI r'GATC_y
Cuts at: 0 659 1407
Size: 659 748
BstZ17I GTA'TAC
Cuts at: 0 1104 1407
Size: 1104 303
BtgI C'CryG_G
Cuts at: 0 476 710 917 1319 1407
Size: 476 234 207 402 88
Fragments arranged by size:
476 402 234 207 88
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 864 1203 1407
Size: 864 339 204
Fragments arranged by size:
864 339 204
BtsI GCAGTG_nn'
Cuts at: 0 518 1407
Size: 518 889
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 93 128 1407
Size: 93 35 1279
Fragments arranged by size:
1279 93 35
EaeI y'GGCC_r
Cuts at: 0 8 101 588 606 638 671 1316 1407
Size: 8 93 487 18 32 33 645 91
Fragments arranged by size:
645 487 93 91 33 32 18 8
EagI C'GGCC_G
Cuts at: 0 588 606 1316 1407
Size: 588 18 710 91
Fragments arranged by size:
710 588 91 18
EarI CTCTTCn'nnn_
Cuts at: 0 76 313 361 559 1407
Size: 76 237 48 198 848
Fragments arranged by size:
848 237 198 76 48
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 57 115 181 583 1059 1062 1407
Size: 57 58 66 402 476 3 345
Fragments arranged by size:
476 402 345 66 58 57 3
EcoNI CCTnn'n_nnAGG
Cuts at: 0 94 1096 1407
Size: 94 1002 311
Fragments arranged by size:
1002 311 94
EcoO109I rG'GnC_Cy
Cuts at: 0 1296 1407
Size: 1296 111
HaeII r_GCGC'y
Cuts at: 0 376 649 953 1033 1198 1306 1377 1407
Size: 376 273 304 80 165 108 71 30
Fragments arranged by size:
376 304 273 165 108 80 71 30
KasI G'GCGC_C
Cuts at: 0 1302 1407
Size: 1302 105
MluI A'CGCG_T
Cuts at: 0 119 1106 1407
Size: 119 987 301
Fragments arranged by size:
987 301 119
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1163 1407
Size: 1163 244
MslI CAynn'nnrTG
Cuts at: 0 253 493 523 529 1240 1407
Size: 253 240 30 6 711 167
Fragments arranged by size:
711 253 240 167 30 6
MspA1I CmG'CkG
Cuts at: 0 203 626 837 919 1145 1321 1367 1407
Size: 203 423 211 82 226 176 46 40
Fragments arranged by size:
423 226 211 203 176 82 46 40
NarI GG'CG_CC
Cuts at: 0 1303 1407
Size: 1303 104
NcoI C'CATG_G
Cuts at: 0 476 1407
Size: 476 931
NheI G'CTAG_C
Cuts at: 0 60 1407
Size: 60 1347
NotI GC'GGCC_GC
Cuts at: 0 1316 1407
Size: 1316 91
NspI r_CATG'y
Cuts at: 0 327 498 1245 1407
Size: 327 171 747 162
Fragments arranged by size:
747 327 171 162
PasI CC'CwG_GG
Cuts at: 0 279 1407
Size: 279 1128
PciI A'CATG_T
Cuts at: 0 494 1407
Size: 494 913
PflMI CCAn_nnn'nTGG
Cuts at: 0 549 1407
Size: 549 858
PfoI T'CCnGG_A
Cuts at: 0 83 752 1407
Size: 83 669 655
Fragments arranged by size:
669 655 83
PstI C_TGCA'G
Cuts at: 0 131 1295 1407
Size: 131 1164 112
Fragments arranged by size:
1164 131 112
PvuII CAG'CTG
Cuts at: 0 626 1145 1407
Size: 626 519 262
Fragments arranged by size:
626 519 262
SacII CC_GC'GG
Cuts at: 0 920 1322 1407
Size: 920 402 85
Fragments arranged by size:
920 402 85
SapI GCTCTTCn'nnn_
Cuts at: 0 313 559 1407
Size: 313 246 848
Fragments arranged by size:
848 313 246
SfcI C'TryA_G
Cuts at: 0 127 1291 1362 1407
Size: 127 1164 71 45
Fragments arranged by size:
1164 127 71 45
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 679 1407
Size: 679 728
SfoI GGC'GCC
Cuts at: 0 1304 1407
Size: 1304 103
SmaI CCC'GGG
Cuts at: 0 197 235 1407
Size: 197 38 1172
Fragments arranged by size:
1172 197 38
SmlI C'TyrA_G
Cuts at: 0 1198 1407
Size: 1198 209
SphI G_CATG'C
Cuts at: 0 327 1245 1407
Size: 327 918 162
Fragments arranged by size:
918 327 162
StyI C'CwwG_G
Cuts at: 0 476 641 1407
Size: 476 165 766
Fragments arranged by size:
766 476 165
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 255 1407
Size: 255 1152
TatI w'GTAC_w
Cuts at: 0 260 1407
Size: 260 1147
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 130 162 1407
Size: 130 32 1245
Fragments arranged by size:
1245 130 32
XmaI C'CCGG_G
Cuts at: 0 195 233 1407
Size: 195 38 1174
Fragments arranged by size:
1174 195 38
ZraI GAC'GTC
Cuts at: 0 485 1407
Size: 485 922
Enzymes that do cut:
AatII AccI AcuI AflIII AlwNI AscI AvaI BanI
BbeI BglI Bme1580I BmrI BmtI BpmI BpuEI BsaBI
BsaHI BsaWI BseRI BseYI BsiEI BsiHKAI BsmBI Bsp1286I
BspEI BspMI BssHII BssSI BstAPI BstEII BstYI BstZ17I
BtgI BtgZI BtsI CspCI EaeI EagI EarI Eco57MI
EcoNI EcoO109I HaeII KasI MluI MmeI MslI MspA1I
NarI NcoI NheI NotI NspI PasI PciI PflMI
PfoI PstI PvuII SacII SapI SfcI SfiI SfoI
SmaI SmlI SphI StyI TaqII TatI TstI XmaI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AseI AsiSI AvrII
BaeI BamHI BanII BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BmgBI BplI Bpu10I BsaI BsaAI
BsaXI BsgI BsiWI BsmI BspHI BsrBI BsrDI BsrFI
BsrGI BstBI BstXI Bsu36I ClaI DraI DraIII DrdI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MfeI MscI NaeI
NdeI NgoMIV NruI NsiI PacI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SalI SanDI SbfI ScaI SexAI SgfI SgrAI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmnI