################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII AvrII BanI BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BlnI BpmI BpuAI BsaAI BsaBI BsaI BsaWI BseAI BsgI BsiHKAI BsiWI BsmFI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eam1105I EarI Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeI MamI MfeI MluI MroI MslI MunI MwoI NarI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuII RcaI RsaI RsrII SacI SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccII 126 CGCG CG^CG AcsI 328 AAATTC [GA]^AATT[CT] AcyI 595 GACGCC G[GA]^CG[CT]C ApaI 475 GGGCCC GGGCC^C ApoI 328 AAATTC [GA]^AATT[CT] Asp700I 69 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BalI 524 TGGCCA TGG^CCA BamHI 351 GGATCC G^GATCC BanII 475 GGGCCC G[GA]GC[CT]^C BcnI 48 CCCGG CC^[GC]GG BglII 285 AGATCT A^GATCT BlpI 28 GCTCAGC GC^T[ACGT]AGC BmyI 475 GGGCCC G[AGT]GC[ACT]^C Bpu1102I 28 GCTCAGC GC^T[ACGT]AGC BsaHI 595 GACGCC G[GA]^CG[CT]C BseRI 152 GAGGAG GAGGAG(10/8) BsmBI 556 GAGACG CGTCTC(1/5) Bsp1286I 475 GGGCCC G[AGT]GC[ACT]^C BsrBI 479 CCGCTC CCGCTC(-3/-3) BsrFI 102 GCCGGC [GA]^CCGG[CT] BsrI 348 ACTGG ACTGG(1/-1) BstNI 599 CCAGG CC^[AT]GG BstUI 126 CGCG CG^CG CelII 28 GCTCAGC GC^T[ACGT]AGC Cfr10I 102 GCCGGC [GA]^CCGG[CT] DraII 415 GGGTCCT [GA]G^G[ACGT]CC[CT] EagI 104 CGGCCG C^GGCCG EclXI 104 CGGCCG C^GGCCG Eco52I 104 CGGCCG C^GGCCG EcoNI 315 CCTGCCGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 415 GGGTCCT [GA]G^G[ACGT]CC[CT] EcoRII 599 CCAGG ^CC[AT]GG HgaI 595 GACGC GACGC(5/10) Hin1I 595 GACGCC G[GA]^CG[CT]C KspI 125 CCGCGG CCGC^GG MaeIII 257 GTCAC ^GT[ACGT]AC MluNI 524 TGGCCA TGG^CCA MscI 524 TGGCCA TGG^CCA MseI 280 TTAA T^TAA MspA1I 125 CCGCGG C[AC]G^C[GT]G MvaI 599 CCAGG CC^[AT]GG MvnI 126 CGCG CG^CG NaeI 102 GCCGGC GCC^GGC NciI 48 CCCGG CC^[GC]GG NgoMIV 102 GCCGGC G^CCGGC NspI 404 GCATGC [GA]CATG^[CT] PpuMI 415 GGGTCCT [GA]G^G[AT]CC[CT] PvuI 462 CGATCG CGAT^CG SacII 125 CCGCGG CCGC^GG SphI 404 GCATGC GCATG^C StuI 216 AGGCCT AGG^CCT TaqI 148 TCGA T^CGA TfiI 562 GAATC G^A[AT]TC Tru9I 280 TTAA T^TAA Tsp45I 257 GTCAC ^GT[GC]AC TthHB8I 148 TCGA T^CGA XmnI 69 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AluI 53 AGCT AG^CT 141 AGCT Aor51HI 336 AGCGCT AGC^GCT 390 AGCGCT AvaII 122 GGTCC G^G[AT]CC 416 GGTCC BsiEI 104 CGGCCG CG[GA][CT]^CG 462 CGATCG BsiYI 315 CCTGCCGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 516 CCGCTACTTGG BslI 315 CCTGCCGAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 516 CCGCTACTTGG BsmAI 556 GAGAC GTCTC(1/5) 546 GTCTC BssKI 48 CCCGG ^CC[ACGT]GG 599 CCAGG DdeI 29 CTCAG C^T[ACGT]AG 537 CTCAG DsaI 125 CCGCGG C^C[GA][CT]GG 191 CCACGG EaeI 104 CGGCCG [CT]^GGCC[GA] 524 TGGCCA Eco47III 336 AGCGCT AGC^GCT 390 AGCGCT EcoT14I 80 CCAAGG C^C[AT][AT]GG 551 CCTTGG HaeII 336 AGCGCT [GA]GCGC^[CT] 390 AGCGCT HphI 77 TCACC GGTGA(8/7) 258 TCACC ScrFI 48 CCCGG CC^[ACGT]GG 599 CCAGG SfaNI 398 GATGC GCATC(5/9) 485 GCATC SmlI 224 CTCAAG C^T[CT][GA]AG 289 CTTGAG StyI 80 CCAAGG C^C[AT][AT]GG 551 CCTTGG TspRI 253 CAGTG CA[GC]TG[ACGT][ACGT]^ 426 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AciI AlwI BbvI BsaJI BstF5I BstYI Cac8I CfoI Cfr13I DpnII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII ItaI MaeII MboI MboII MflI MnlI MspI NdeII NlaIII NlaIV PleI Sau3AI Sau96I TseI Tsp509I XhoII