################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AfaI AflII AflIII AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AviII AvrII BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BseAI BseRI BsgI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr13I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FbaI FokI FseI FspI HgaI HincII HindII HindIII HpaI KpnI Ksp632I KspI MaeI MaeII MamI MboI MboII MfeI MflI MluI MroI MseI MslI MspA1I MunI NcoI NdeI NdeII NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacII SalI SapI Sau3AI Sau96I SbfI ScaI SexAI SfaNI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccII 53 CGCG CG^CG AcsI 158 GAATTC [GA]^AATT[CT] AcyI 70 GGCGCC G[GA]^CG[CT]C AgeI 35 ACCGGT A^CCGGT AluI 276 AGCT AG^CT ApoI 158 GAATTC [GA]^AATT[CT] AspHI 275 GAGCTC G[AT]GC[AT]^C AvaI 245 CTCGGG C^[CT]CG[GA]G BanI 70 GGCGCC G^G[CT][GA]CC BbeI 70 GGCGCC GGCGC^C BcnI 104 CCGGG CC^[GC]GG BpmI 349 CTCCAG CTGGAG(16/14) BsaHI 70 GGCGCC G[GA]^CG[CT]C BsaI 93 GGTCTC GGTCTC(1/5) BsaWI 35 ACCGGT [AT]^CCGG[AT] BsiEI 101 CGACCG CG[GA][CT]^CG BsiHKAI 275 GAGCTC G[AT]GC[AT]^C BsoBI 245 CTCGGG C^[CT]CG[GA]G BstUI 53 CGCG CG^CG EcoRI 158 GAATTC G^AATTC HaeII 70 GGCGCC [GA]GCGC^[CT] Hin1I 70 GGCGCC G[GA]^CG[CT]C HinfI 243 GACTC G^A[ACGT]TC KasI 70 GGCGCC G^GCGCC MvnI 53 CGCG CG^CG NaeI 23 GCCGGC GCC^GGC NarI 70 GGCGCC GG^CGCC NciI 104 CCGGG CC^[GC]GG NgoMIV 23 GCCGGC G^CCGGC NlaIII 332 CATG CATG^ NspI 331 ACATGC [GA]CATG^[CT] PinAI 35 ACCGGT A^CCGGT PleI 243 GACTC GAGTC(4/5) SacI 275 GAGCTC GAGCT^C SfcI 150 CTACAG C^T[GA][CT]AG SfoI 70 GGCGCC GGC^GCC Tsp45I 115 GTCAC ^GT[GC]AC Tsp509I 159 AATT ^AATT TspRI 123 CACTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BalI 126 TGGCCA TGG^CCA 282 TGGCCA BanII 275 GAGCTC G[GA]GC[CT]^C 346 GGGCTC BbvI 222 GCAGC GCAGC(8/12) 27 GCTGC BmyI 275 GAGCTC G[AGT]GC[ACT]^C 346 GGGCTC BsiYI 17 CCTTCTGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 60 CCTGCCCCTGG BslI 17 CCTTCTGCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 60 CCTGCCCCTGG BsmAI 241 GAGAC GTCTC(1/5) 94 GTCTC Bsp1286I 275 GAGCTC G[AGT]GC[ACT]^C 346 GGGCTC BsrI 124 ACTGG ACTGG(1/-1) 351 CCAGT CfoI 52 GCGC GCG^C 71 GCGC EaeI 126 TGGCCA [CT]^GGCC[GA] 282 TGGCCA FauI 90 GCGGG CCCGC(4/6) 269 GCGGG HaeIII 127 GGCC GG^CC 283 GGCC HhaI 52 GCGC GCG^C 71 GCGC HinP1I 52 GCGC G^CGC 71 GCGC HphI 186 GGTGA GGTGA(8/7) 249 GGTGA MaeIII 115 GTCAC ^GT[ACGT]AC 338 GTTAC MluNI 126 TGGCCA TGG^CCA 282 TGGCCA MscI 126 TGGCCA TGG^CCA 282 TGGCCA TseI 27 GCTGC G^C[AT]GC 222 GCAGC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AciI BsaJI BsrFI BssKI BstNI Cac8I Cfr10I EcoRII Fnu4HI HapII HpaII ItaI MnlI MspI MvaI MwoI NlaIV ScrFI