(Circular) (Six-base) MAPSORT of: pFN21AE2147.seq Check: 4251 from: 1 to: 5077 pFN21AE2147 5077 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2887 278 Size: 53 83 186 936 1351 2468 Fragments arranged by size: 2468 1351 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2420 1937 Size: 483 4594 Acc65I G'GTAC_C Cuts at: 2398 2398 Size: 5077 AclI AA'CG_TT Cuts at: 1563 3204 3577 1563 Size: 1641 373 3063 Fragments arranged by size: 3063 1641 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3140 4524 4792 1632 Size: 1508 1384 268 1917 Fragments arranged by size: 1917 1508 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4880 AflIII A'CryG_T Cuts at: 1203 3976 4953 1203 Size: 2773 977 1327 Fragments arranged by size: 2773 1327 977 AgeI A'CCGG_T Cuts at: 2040 3908 2040 Size: 1868 3209 AhdI GACnn_n'nnGTC Cuts at: 3806 3806 Size: 5077 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5077 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5077 AlwNI CAG_nnn'CTG Cuts at: 2820 4392 2820 Size: 1572 3505 ApaLI G'TGCA_C Cuts at: 3134 4290 3134 Size: 1156 3921 ApoI r'AATT_y Cuts at: 1493 2163 2386 2612 2764 4873 1493 Size: 670 223 226 152 2109 1697 Fragments arranged by size: 2109 1697 670 226 223 152 AseI AT'TA_AT Cuts at: 160 1032 3631 160 Size: 872 2599 1606 Fragments arranged by size: 2599 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5077 AvaI C'yCGr_G Cuts at: 1870 1943 2250 2402 1870 Size: 73 307 152 4545 Fragments arranged by size: 4545 307 152 73 BamHI G'GATC_C Cuts at: 2407 2774 2407 Size: 367 4710 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2075 2398 3853 618 Size: 325 217 627 288 323 1455 1842 Fragments arranged by size: 1842 1455 627 325 323 288 217 BanII G_rGCy'C Cuts at: 1391 2284 2355 2396 1391 Size: 893 71 41 4072 Fragments arranged by size: 4072 893 71 41 BbeI G_GCGC'C Cuts at: 2079 2079 Size: 5077 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4106 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3268 3302 1903 Size: 34 1331 34 3678 Fragments arranged by size: 3678 1331 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2969 4179 2969 Size: 1210 3867 BclI T'GATC_A Cuts at: 1548 1548 Size: 5077 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 3688 136 Size: 107 122 71 631 605 337 1679 1525 Fragments arranged by size: 1679 1525 631 605 337 122 107 71 BglII A'GATC_T Cuts at: 5072 5072 Size: 5077 BlpI GC'TnA_GC Cuts at: 2494 2494 Size: 5077 Bme1580I G_kGCm'C Cuts at: 1790 3138 4294 1790 Size: 1348 1156 2573 Fragments arranged by size: 2573 1348 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5077 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2808 3766 442 Size: 416 53 83 399 24 1391 958 1753 Fragments arranged by size: 1753 1391 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5077 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2339 3737 1239 Size: 1100 1398 2579 Fragments arranged by size: 2579 1398 1100 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3202 4067 4365 3202 Size: 865 298 3914 Fragments arranged by size: 3914 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2104 3740 882 Size: 617 605 1636 2219 Fragments arranged by size: 2219 1636 617 605 BsaAI yAC'GTr Cuts at: 493 1204 2868 493 Size: 711 1664 2702 Fragments arranged by size: 2702 1664 711 BsaBI GATnn'nnATC Cuts at: 5071 5071 Size: 5077 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2076 2884 3266 3901 Size: 53 83 186 936 543 808 382 635 Cuts at: 3901 275 Size: 1451 Fragments arranged by size: 1451 936 808 635 543 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2040 3510 3908 4182 4329 4740 2040 Size: 1470 398 274 147 411 2377 Fragments arranged by size: 2377 1470 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4852 Fragments arranged by size: 4852 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5077 BseYI C'CCAG_C Cuts at: 4280 4776 4280 Size: 496 4581 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4504 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2109 2527 3288 4316 1540 Size: 275 186 108 418 761 1028 2301 Fragments arranged by size: 2301 1028 761 418 275 186 108 BsiHKAI G_wGCw'C Cuts at: 2284 3138 3223 4294 2284 Size: 854 85 1071 3067 Fragments arranged by size: 3067 1071 854 85 BsmI GAATG_Cn' Cuts at: 2588 2681 2588 Size: 93 4984 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2284 2355 2396 3138 3223 4294 1391 Size: 399 494 71 41 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 494 399 85 71 41 BspEI T'CCGG_A Cuts at: 4740 4740 Size: 5077 BspHI T'CATG_A Cuts at: 2966 4696 2966 Size: 1730 3347 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2432 844 Size: 1588 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2964 1394 Size: 429 1141 3507 Fragments arranged by size: 3507 1141 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3572 3746 65 Size: 1183 2324 174 1396 Fragments arranged by size: 2324 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2028 2040 2330 3721 3908 1700 Size: 110 218 12 290 1391 187 2869 Fragments arranged by size: 2869 1391 290 218 187 110 12 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5077 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5077 BssSI C'ACGA_G Cuts at: 3137 4149 3137 Size: 1012 4065 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5077 BstBI TT'CG_AA Cuts at: 3881 3881 Size: 5077 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5077 BstYI r'GATC_y Cuts at: 1972 2407 2774 3159 3176 4628 4714 4726 5072 Size: 435 367 385 17 1452 86 12 346 Cuts at: 5072 1972 Size: 1977 Fragments arranged by size: 1977 1452 435 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3586 Fragments arranged by size: 3586 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2030 2280 487 Size: 683 56 804 250 3284 Fragments arranged by size: 3284 804 683 250 56 BtsI GCAGTG_nn' Cuts at: 2593 3387 3407 2593 Size: 794 20 4263 Fragments arranged by size: 4263 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5077 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5042 DraI TTT'AAA Cuts at: 2381 2381 Size: 5077 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4084 809 Size: 507 2768 1802 Fragments arranged by size: 2768 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2131 2287 2524 Size: 54 1425 50 275 18 301 156 237 Cuts at: 2524 3413 5042 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 301 275 237 156 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2524 1537 Size: 275 712 4090 Fragments arranged by size: 4090 712 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3007 1353 Size: 1654 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3664 4038 4184 387 Size: 1064 138 2075 374 146 1280 Fragments arranged by size: 2075 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2282 2282 Size: 5077 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2339 3140 3737 4524 4792 1239 Size: 393 707 801 597 787 268 1524 Fragments arranged by size: 1524 801 787 707 597 393 268 EcoRI G'AATT_C Cuts at: 2163 2386 2764 2163 Size: 223 378 4476 Fragments arranged by size: 4476 378 223 FspI TGC'GCA Cuts at: 3583 3583 Size: 5077 HaeII r_GCGC'y Cuts at: 1678 2079 4224 1678 Size: 401 2145 2531 Fragments arranged by size: 2531 2145 401 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3718 3750 3792 3824 1373 Size: 32 135 32 2146 32 42 32 2626 Fragments arranged by size: 2626 2146 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2421 2667 669 Size: 988 281 483 246 3079 Fragments arranged by size: 3079 988 483 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5077 HpaI GTT'AAC Cuts at: 2667 2667 Size: 5077 KasI G'GCGC_C Cuts at: 2075 2075 Size: 5077 KpnI G_GTAC'C Cuts at: 2402 2402 Size: 5077 MfeI C'AATT_G Cuts at: 2676 2676 Size: 5077 MluI A'CGCG_T Cuts at: 4953 4953 Size: 5077 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4191 4375 1305 Size: 2886 184 2007 Fragments arranged by size: 2886 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2133 2289 10 Size: 54 1425 343 301 156 2798 Fragments arranged by size: 2798 1425 343 301 156 54 MslI CAynn'nnrTG Cuts at: 518 1166 3035 3394 3553 518 Size: 648 1869 359 159 2042 Fragments arranged by size: 2042 1869 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2493 3170 4318 4563 1606 Size: 887 677 1148 245 2120 Fragments arranged by size: 2120 1148 887 677 245 NaeI GCC'GGC Cuts at: 2030 2030 Size: 5077 NarI GG'CG_CC Cuts at: 2076 2076 Size: 5077 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3586 Fragments arranged by size: 3586 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5077 NgoMIV G'CCGG_C Cuts at: 2028 2028 Size: 5077 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5077 NotI GC'GGCC_GC Cuts at: 2524 2524 Size: 5077 NspI r_CATG'y Cuts at: 1132 1229 2340 2435 3980 1132 Size: 97 1111 95 1545 2229 Fragments arranged by size: 2229 1545 1111 97 95 PasI CC'CwG_GG Cuts at: 1348 2071 1348 Size: 723 4354 PciI A'CATG_T Cuts at: 3976 3976 Size: 5077 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5077 PmeI GTTT'AAAC Cuts at: 2381 2381 Size: 5077 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3146 3178 4659 4691 3146 Size: 32 1481 32 3532 Fragments arranged by size: 3532 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5077 PsiI TTA'TAA Cuts at: 2647 2647 Size: 5077 PstI C_TGCA'G Cuts at: 830 2429 830 Size: 1599 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5077 SacI G_AGCT'C Cuts at: 2284 2284 Size: 5077 SalI G'TCGA_C Cuts at: 1936 2419 1936 Size: 483 4594 SbfI CC_TGCA'GG Cuts at: 2429 2429 Size: 5077 ScaI AGT'ACT Cuts at: 3325 5037 3325 Size: 1712 3365 SfcI C'TryA_G Cuts at: 826 1045 2155 2425 3560 4241 4432 826 Size: 219 1110 270 1135 681 191 1471 Fragments arranged by size: 1471 1135 1110 681 270 219 191 SfoI GGC'GCC Cuts at: 2077 2077 Size: 5077 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5077 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5077 SmaI CCC'GGG Cuts at: 1872 2404 1872 Size: 532 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3181 4082 4344 820 Size: 197 926 1238 901 262 1553 Fragments arranged by size: 1553 1238 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2868 493 Size: 2375 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5077 SphI G_CATG'C Cuts at: 1132 2435 1132 Size: 1303 3774 SspI AAT'ATT Cuts at: 5 52 3001 5 Size: 47 2949 2081 Fragments arranged by size: 2949 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5077 StyI C'CwwG_G Cuts at: 513 1065 2004 2516 513 Size: 552 939 512 3074 Fragments arranged by size: 3074 939 552 512 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2307 3104 3121 3274 3459 3904 325 Size: 1255 727 797 17 153 185 445 1498 Fragments arranged by size: 1498 1255 797 727 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3323 5035 96 Size: 274 80 33 51 1443 1346 1712 138 Fragments arranged by size: 1712 1443 1346 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5037 5069 5037 Size: 32 5045 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5077 XbaI T'CTAG_A Cuts at: 2413 2413 Size: 5077 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5077 XmaI C'CCGG_G Cuts at: 1870 2402 1870 Size: 532 4545 XmnI GAAnn'nnTTC Cuts at: 3206 3206 Size: 5077 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2885 276 Size: 53 83 186 936 1351 2468 Fragments arranged by size: 2468 1351 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI