################################## # NO CUT ENZYMES ( 171 enzymes) # ################################## AatII Acc65I AccI AccIII AciI AclI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AspI AvaI AviII AvrII BamHI BanI BanII BbeI BbrPI BbsI BbvCI BclI BcnI BfrI BglI BglII BlnI BmyI BpmI BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI Bsp1286I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstXI BstYI BstZ17I Bsu36I Cfr10I ClaI CpoI DpnII DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI KspI MaeII MamI MboI MfeI MflI MluI MroI MspI MunI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIV NotI NruI NsiI NspI PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PshAI Psp1406I PstI PvuI RcaI RsrII SacI SacII SalI Sau3AI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I Van91I XbaI XhoI XhoII XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 880 CGCG CG^CG AlwNI 34 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG AseI 205 ATTAAT AT^TAAT AsnI 205 ATTAAT AT^TAAT AvaII 297 GGACC G^G[AT]CC BalI 942 TGGCCA TGG^CCA BcgI 175 GCAAGGAAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 482 GTATCC GTATCC(6/5) BfaI 824 CTAG C^TAG BlpI 231 GCTAAGC GC^T[ACGT]AGC Bpu1102I 231 GCTAAGC GC^T[ACGT]AGC BsmI 258 GAATGC GAATGC(1/-1) Bsp1407I 526 TGTACA T^GTACA BsrDI 602 CATTGC GCAATG(2/0) BsrGI 526 TGTACA T^GTACA BssKI 486 CCTGG ^CC[ACGT]GG BstBI 866 TTCGAA TT^CGAA BstNI 486 CCTGG CC^[AT]GG BstUI 880 CGCG CG^CG CelII 231 GCTAAGC GC^T[ACGT]AGC CfoI 881 GCGC GCG^C Cfr13I 297 GGACC G^G[ACGT]CC DraII 296 AGGACCT [GA]G^G[ACGT]CC[CT] EaeI 942 TGGCCA [CT]^GGCC[GA] EarI 673 GAAGAG CTCTTC(1/4) EcoO109I 296 AGGACCT [GA]G^G[ACGT]CC[CT] EcoRII 486 CCTGG ^CC[AT]GG HhaI 881 GCGC GCG^C HinP1I 881 GCGC G^CGC HindIII 234 AAGCTT A^AGCTT Ksp632I 673 GAAGAG CTCTTC(1/4) MaeI 824 CTAG C^TAG MluNI 942 TGGCCA TGG^CCA MscI 942 TGGCCA TGG^CCA MvaI 486 CCTGG CC^[AT]GG MvnI 880 CGCG CG^CG MwoI 222 GCCTCCTGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NspV 866 TTCGAA TT^CGAA PpuMI 296 AGGACCT [GA]G^G[AT]CC[CT] PshBI 205 ATTAAT AT^TAAT SapI 673 GAAGAGC GCTCTTC(1/4) Sau96I 297 GGACC G^G[ACGT]CC ScrFI 486 CCTGG CC^[ACGT]GG SfcI 54 CTACAG C^T[GA][CT]AG SfuI 866 TTCGAA TT^CGAA SspBI 526 TGTACA T^GTACA SspI 892 AATATT AAT^ATT Tsp45I 350 GTCAC ^GT[GC]AC XcmI 476 CCAAGGGTATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AcsI 646 GAATTT [GA]^AATT[CT] 859 GAATTT AfaI 527 GTAC GT^AC 784 GTAC ApoI 646 GAATTT [GA]^AATT[CT] 859 GAATTT Asp700I 646 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 859 GAATTTATTC BsaJI 141 CCAAGG C^C[ACGT][ACGT]GG 476 CCAAGG Cac8I 103 GCGAGC GC[ACGT]^[ACGT]GC 730 GCTTGC EcoT14I 141 CCAAGG C^C[AT][AT]GG 476 CCAAGG HaeIII 760 GGCC GG^CC 943 GGCC HphI 74 TCACC GGTGA(8/7) 431 TCACC MaeIII 350 GTCAC ^GT[ACGT]AC 598 GTAAC MslI 658 CATATCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 916 CATCTTTATG MspA1I 37 CAGCTG C[AC]G^C[GT]G 412 CAGCTG PvuII 37 CAGCTG CAG^CTG 412 CAGCTG RsaI 527 GTAC GT^AC 784 GTAC SfaNI 772 GATGC GCATC(5/9) 915 GCATC StyI 141 CCAAGG C^C[AT][AT]GG 476 CCAAGG TaqI 184 TCGA T^CGA 867 TCGA TthHB8I 184 TCGA T^CGA 867 TCGA XmnI 646 GAATTTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 859 GAATTTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 19 enzymes) # ######################################### AluI BbvI BsiYI BslI BstF5I DdeI Fnu4HI FokI HinfI ItaI MboII MnlI MseI NlaIII TfiI Tru9I TseI Tsp509I TspRI