################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspHI AspI AvaI AviII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BclI BcnI BfrI BglI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsoBI BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII KasI KpnI KspI MaeII MamI MluI MluNI MroI MscI MslI MspA1I MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I StuI SwaI TaqI Tth111I TthHB8I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AciI 1148 GCGG CCGC(-3/-1) Asp700I 1364 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaII 1198 GGTCC G^G[AT]CC AvrII 1017 CCTAGG C^CTAGG BanII 445 GAGCCC G[GA]GC[CT]^C BciVI 124 GTATCC GTATCC(6/5) BlnI 1017 CCTAGG C^CTAGG BsiYI 27 CCTCCAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 27 CCTCCAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 928 GTCTC GTCTC(1/5) BsmFI 47 GTCCC GGGAC(10/14) BsmI 279 GAATGC GAATGC(1/-1) Bsp1407I 346 TGTACA T^GTACA BsrGI 346 TGTACA T^GTACA BstF5I 913 CATCC GGATG(2/0) BstXI 50 CCATCTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr13I 1198 GGTCC G^G[ACGT]CC DraII 1197 AGGTCCT [GA]G^G[ACGT]CC[CT] EaeI 1041 TGGCCG [CT]^GGCC[GA] EcoNI 27 CCTCCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1197 AGGTCCT [GA]G^G[ACGT]CC[CT] FokI 913 CATCC GGATG(9/13) HgaI 1192 GCGTC GACGC(5/10) MfeI 1160 CAATTG C^AATTG MunI 1160 CAATTG C^AATTG NlaIV 200 GGAACC GG[ACGT]^[ACGT]CC PleI 37 GAGTC GAGTC(4/5) PpuMI 1197 AGGTCCT [GA]G^G[AT]CC[CT] Sau96I 1198 GGTCC G^G[ACGT]CC ScaI 62 AGTACT AGT^ACT SspBI 346 TGTACA T^GTACA SspI 845 AATATT AAT^ATT XmnI 1364 GAACAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 425 AAATTC [GA]^AATT[CT] 549 AAATTC AfaI 63 GTAC GT^AC 347 GTAC AlwI 135 GGATC GGATC(4/5) 1128 GGATC ApoI 425 AAATTC [GA]^AATT[CT] 549 AAATTC BbsI 833 GTCTTC GAAGAC(2/6) 1109 GTCTTC BbvI 497 GCAGC GCAGC(8/12) 406 GCTGC BmyI 94 GGGCAC G[AGT]GC[ACT]^C 445 GAGCCC BpmI 241 CTCCAG CTGGAG(16/14) 58 CTGGAG BpuAI 833 GTCTTC GAAGAC(2/6) 1109 GTCTTC BsgI 354 GTGCAG GTGCAG(16/14) 724 GTGCAG Bsp1286I 94 GGGCAC G[AGT]GC[ACT]^C 445 GAGCCC EcoT14I 988 CCAAGG C^C[AT][AT]GG 1017 CCTAGG Fnu4HI 406 GCTGC GC^[ACGT]GC 497 GCAGC HaeIII 1042 GGCC GG^CC 1346 GGCC HphI 21 GGTGA GGTGA(8/7) 1270 GGTGA ItaI 406 GCTGC GC^[ACGT]GC 497 GCAGC MaeIII 10 GTGAC ^GT[ACGT]AC 79 GTGAC MwoI 167 GCCGTTTTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1125 GCAGGATCAGC NlaIII 285 CATG CATG^ 1173 CATG RsaI 63 GTAC GT^AC 347 GTAC SmlI 442 CTTGAG C^T[CT][GA]AG 450 CTTGAG StyI 988 CCAAGG C^C[AT][AT]GG 1017 CCTAGG TseI 406 GCTGC G^C[AT]GC 497 GCAGC Tsp45I 10 GTGAC ^GT[GC]AC 79 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 32 enzymes) # ######################################### AluI AlwNI BfaI BglII BsaJI BseRI BsrI BssKI BstNI BstYI DdeI DpnII EarI EcoRII HinfI Ksp632I MaeI MboI MboII MflI MnlI MseI MvaI NdeII Sau3AI ScrFI TfiI Tru9I Tsp509I TspRI XbaI XhoII