(Circular) (Six-base) MAPSORT of: pFN21AE4102.seq Check: 9956 from: 1 to: 6118 pFN21AE4102 6118 bp With 182 enzymes: SgfI * March 28, 2012 13:07 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3928 278 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3461 1937 Size: 1524 4594 Acc65I G'GTAC_C Cuts at: 3439 3439 Size: 6118 AclI AA'CG_TT Cuts at: 1563 4245 4618 1563 Size: 2682 373 3063 Fragments arranged by size: 3063 2682 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2182 4181 5565 5833 1632 Size: 550 1999 1384 268 1917 Fragments arranged by size: 1999 1917 1384 550 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5921 AflIII A'CryG_T Cuts at: 1203 5017 5994 1203 Size: 3814 977 1327 Fragments arranged by size: 3814 1327 977 AgeI A'CCGG_T Cuts at: 4949 4949 Size: 6118 AhdI GACnn_n'nnGTC Cuts at: 4847 4847 Size: 6118 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6118 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2744 1184 Size: 1560 4558 AlwNI CAG_nnn'CTG Cuts at: 2314 3223 3410 3861 5433 2314 Size: 909 187 451 1572 2999 Fragments arranged by size: 2999 1572 909 451 187 ApaLI G'TGCA_C Cuts at: 4175 5331 4175 Size: 1156 4962 ApoI r'AATT_y Cuts at: 1493 2430 2554 3427 3653 3805 5914 1493 Size: 937 124 873 226 152 2109 1697 Fragments arranged by size: 2109 1697 937 873 226 152 124 AseI AT'TA_AT Cuts at: 160 1032 4672 160 Size: 872 3640 1606 Fragments arranged by size: 3640 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 AvaI C'yCGr_G Cuts at: 1870 1943 3443 1870 Size: 73 1500 4545 Fragments arranged by size: 4545 1500 73 AvrII C'CTAG_G Cuts at: 3022 3022 Size: 6118 BamHI G'GATC_C Cuts at: 3448 3815 3448 Size: 367 5751 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3439 4894 618 Size: 325 217 627 1652 1455 1842 Fragments arranged by size: 1842 1652 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2454 3437 1391 Size: 1063 983 4072 Fragments arranged by size: 4072 1063 983 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2831 3107 928 Size: 971 932 276 3939 Fragments arranged by size: 3939 971 932 276 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4309 4343 1903 Size: 34 2372 34 3678 Fragments arranged by size: 3678 2372 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2140 4010 5220 2140 Size: 1870 1210 3038 Fragments arranged by size: 3038 1870 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6118 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4729 136 Size: 107 122 71 631 605 3057 1525 Fragments arranged by size: 3057 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2335 2343 2902 6113 2335 Size: 8 559 3211 2340 Fragments arranged by size: 3211 2340 559 8 BlpI GC'TnA_GC Cuts at: 3535 3535 Size: 6118 Bme1580I G_kGCm'C Cuts at: 1790 2103 4179 5335 1790 Size: 313 2076 1156 2573 Fragments arranged by size: 2573 2076 1156 313 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6118 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2075 3067 3234 Size: 416 53 83 399 24 658 992 167 Cuts at: 3234 3849 4807 442 Size: 615 958 1753 Fragments arranged by size: 1753 992 958 658 615 416 399 167 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6118 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2231 4778 1239 Size: 845 147 2547 2579 Fragments arranged by size: 2579 2547 845 147 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2468 2476 4243 5108 5406 2468 Size: 8 1767 865 298 3180 Fragments arranged by size: 3180 1767 865 298 8 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4781 882 Size: 617 3282 2219 Fragments arranged by size: 3282 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3909 493 Size: 711 2705 2702 Fragments arranged by size: 2705 2702 711 BsaBI GATnn'nnATC Cuts at: 6112 6112 Size: 6118 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3925 4307 4942 275 Size: 53 83 186 936 2392 382 635 1451 Fragments arranged by size: 2392 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4551 4949 5223 5370 5781 4551 Size: 398 274 147 411 4888 Fragments arranged by size: 4888 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5893 Fragments arranged by size: 5893 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2054 2272 2835 1186 Size: 868 218 563 4469 Fragments arranged by size: 4469 868 563 218 BseYI C'CCAG_C Cuts at: 2211 3216 5321 5817 2211 Size: 1005 2105 496 2512 Fragments arranged by size: 2512 2105 1005 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2380 2750 1163 Size: 573 644 370 4531 Fragments arranged by size: 4531 644 573 370 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3568 4329 5357 1540 Size: 275 186 1567 761 1028 2301 Fragments arranged by size: 2301 1567 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4179 4264 5335 4179 Size: 85 1071 4962 Fragments arranged by size: 4962 1071 85 BsmI GAATG_Cn' Cuts at: 2290 3629 3722 2290 Size: 1339 93 4686 Fragments arranged by size: 4686 1339 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2103 2454 3437 4179 4264 5335 1391 Size: 399 313 351 983 742 85 1071 2174 Fragments arranged by size: 2174 1071 983 742 399 351 313 85 BspEI T'CCGG_A Cuts at: 5781 5781 Size: 6118 BspHI T'CATG_A Cuts at: 4007 5737 4007 Size: 1730 4388 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3473 844 Size: 2629 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4005 1394 Size: 429 2182 3507 Fragments arranged by size: 3507 2182 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4613 4787 65 Size: 1183 3365 174 1396 Fragments arranged by size: 3365 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4762 4949 1700 Size: 110 2952 187 2869 Fragments arranged by size: 2952 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2351 96 Size: 2255 3863 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6118 BssSI C'ACGA_G Cuts at: 4178 5190 4178 Size: 1012 5106 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6118 BstBI TT'CG_AA Cuts at: 4922 4922 Size: 6118 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6118 BstXI CCAn_nnnn'nTGG Cuts at: 2062 2062 Size: 6118 BstYI r'GATC_y Cuts at: 1972 2140 2335 2343 2902 3448 3815 4200 4217 Size: 168 195 8 559 546 367 385 17 Cuts at: 4217 5669 5755 5767 6113 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 559 546 385 367 346 195 168 86 17 12 8 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4627 Fragments arranged by size: 4627 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5379 Fragments arranged by size: 5379 683 56 BtsI GCAGTG_nn' Cuts at: 3634 4428 4448 3634 Size: 794 20 5304 Fragments arranged by size: 5304 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6118 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6083 DraI TTT'AAA Cuts at: 3422 3422 Size: 6118 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5125 809 Size: 507 3809 1802 Fragments arranged by size: 3809 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3046 3565 4454 Size: 54 1425 50 275 18 1216 519 889 Cuts at: 4454 6083 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1216 889 519 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3565 1537 Size: 275 1753 4090 Fragments arranged by size: 4090 1753 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2327 2622 3176 4048 1353 Size: 974 295 554 872 3423 Fragments arranged by size: 3423 974 872 554 295 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3168 4705 5079 5225 387 Size: 1064 138 1579 1537 374 146 1280 Fragments arranged by size: 1579 1537 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2182 2231 4181 4778 5565 5833 Size: 393 452 98 49 1950 597 787 268 Cuts at: 5833 1239 Size: 1524 Fragments arranged by size: 1950 1524 787 597 452 393 268 98 49 EcoNI CCTnn'n_nnAGG Cuts at: 2036 2036 Size: 6118 EcoO109I rG'GnC_Cy Cuts at: 3203 3203 Size: 6118 EcoRI G'AATT_C Cuts at: 3427 3805 3427 Size: 378 5740 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2462 3131 2462 Size: 669 5449 FspI TGC'GCA Cuts at: 4624 4624 Size: 6118 HaeII r_GCGC'y Cuts at: 1678 5265 1678 Size: 3587 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2708 2740 2887 2919 2991 Size: 32 135 32 1136 32 147 32 72 Cuts at: 2991 3023 3125 3157 4759 4791 4833 4865 1373 Size: 32 102 32 1602 32 42 32 2626 Fragments arranged by size: 2626 1602 1136 147 135 102 72 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3462 3708 669 Size: 988 281 1524 246 3079 Fragments arranged by size: 3079 1524 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6118 HpaI GTT'AAC Cuts at: 3708 3708 Size: 6118 KpnI G_GTAC'C Cuts at: 3443 3443 Size: 6118 MfeI C'AATT_G Cuts at: 3165 3717 3165 Size: 552 5566 MluI A'CGCG_T Cuts at: 5994 5994 Size: 6118 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5232 5416 1305 Size: 3927 184 2007 Fragments arranged by size: 3927 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4296 Fragments arranged by size: 4296 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4076 4435 4594 518 Size: 648 2910 359 159 2042 Fragments arranged by size: 2910 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3534 4211 5359 5604 1606 Size: 1928 677 1148 245 2120 Fragments arranged by size: 2120 1928 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4627 Fragments arranged by size: 4627 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6118 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6118 NotI GC'GGCC_GC Cuts at: 3565 3565 Size: 6118 NspI r_CATG'y Cuts at: 1132 1229 3476 5021 1132 Size: 97 2247 1545 2229 Fragments arranged by size: 2247 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6118 PciI A'CATG_T Cuts at: 5017 5017 Size: 6118 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6118 PfoI T'CCnGG_A Cuts at: 2132 2132 Size: 6118 PmeI GTTT'AAAC Cuts at: 3422 3422 Size: 6118 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3025 3057 4187 4219 5700 5732 3025 Size: 32 1130 32 1481 32 3411 Fragments arranged by size: 3411 1481 1130 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3203 3203 Size: 6118 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6118 PsiI TTA'TAA Cuts at: 3291 3688 3291 Size: 397 5721 PstI C_TGCA'G Cuts at: 830 3470 830 Size: 2640 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6118 SalI G'TCGA_C Cuts at: 1936 3460 1936 Size: 1524 4594 SbfI CC_TGCA'GG Cuts at: 3470 3470 Size: 6118 ScaI AGT'ACT Cuts at: 2069 4366 6078 2069 Size: 2297 1712 2109 Fragments arranged by size: 2297 2109 1712 SfcI C'TryA_G Cuts at: 826 1045 3466 4601 5282 5473 826 Size: 219 2421 1135 681 191 1471 Fragments arranged by size: 2421 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6118 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6118 SmaI CCC'GGG Cuts at: 1872 3445 1872 Size: 1573 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2447 2455 4222 5123 5385 820 Size: 197 926 504 8 1767 901 262 1553 Fragments arranged by size: 1767 1553 926 901 504 262 197 8 SnaBI TAC'GTA Cuts at: 493 3909 493 Size: 3416 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6118 SphI G_CATG'C Cuts at: 1132 3476 1132 Size: 2344 3774 SspI AAT'ATT Cuts at: 5 52 2852 4042 5 Size: 47 2800 1190 2081 Fragments arranged by size: 2800 2081 1190 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6118 StyI C'CwwG_G Cuts at: 513 1065 2004 2993 3022 3557 513 Size: 552 939 989 29 535 3074 Fragments arranged by size: 3074 989 939 552 535 29 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3086 3263 4145 4162 4315 4500 4945 Size: 1255 1506 177 882 17 153 185 445 Cuts at: 4945 325 Size: 1498 Fragments arranged by size: 1506 1498 1255 882 445 185 177 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2067 2351 4364 Size: 274 80 33 51 1443 90 284 2013 Cuts at: 4364 6076 96 Size: 1712 138 Fragments arranged by size: 2013 1712 1443 284 274 138 90 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2902 2934 3082 3114 6078 6110 2902 Size: 32 148 32 2964 32 2910 Fragments arranged by size: 2964 2910 148 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6118 XbaI T'CTAG_A Cuts at: 2143 2338 2434 2899 3454 2143 Size: 195 96 465 555 4807 Fragments arranged by size: 4807 555 465 195 96 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6118 XmaI C'CCGG_G Cuts at: 1870 3443 1870 Size: 1573 4545 XmnI GAAnn'nnTTC Cuts at: 3373 4247 3373 Size: 874 5244 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3926 276 Size: 53 83 186 936 2392 2468 Fragments arranged by size: 2468 2392 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI