Restriction map for
pFN21AE1827
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BamHI BanI
BanII BbeI BbrPI BcgI BclI
BcnI BfaI BfrI BlnI BlpI
Bpu1102I BsaAI BsaHI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FauI FbaI FspI
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeI
MluI MroI MvnI NarI NciI
NcoI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshBI Psp1406I
PstI PvuI RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 156 GTCTAC GT^[AC][GT]AC
AciI 64 GCGG CCGC(-3/-1)
AcsI 85 AAATTC [GA]^AATT[CT]
AgeI 943 ACCGGT A^CCGGT
AlwNI 503 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 85 AAATTC [GA]^AATT[CT]
Asp700I 442 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 811 TGGCCA TGG^CCA
BbvCI 130 CCTCAGC CCTCAGC(-5/-2)
BbvI 746 GCTGC GCAGC(8/12)
BciVI 254 GGATAC GTATCC(6/5)
BglI 651 GCCAACTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 639 AGATCT A^GATCT
BpmI 985 CTGGAG CTGGAG(16/14)
BsaBI 428 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 933 GAGACC GGTCTC(1/5)
BsaWI 943 ACCGGT [AT]^CCGG[AT]
BspMI 551 ACCTGC ACCTGC(4/8)
BsrDI 872 GCAATG GCAATG(2/0)
BssSI 916 CACGAG CACGAG(-5/-1)
BstF5I 471 CATCC GGATG(2/0)
Cac8I 40 GCCGGC GC[ACGT]^[ACGT]GC
CfoI 363 GCGC GCG^C
DraII 920 AGGTCCT [GA]G^G[ACGT]CC[CT]
EarI 7 CTCTTC CTCTTC(1/4)
EcoO109I 920 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoRV 602 GATATC GAT^ATC
Fnu4HI 746 GCTGC GC^[ACGT]GC
FokI 471 CATCC GGATG(9/13)
FseI 39 GGCCGGCC GGCCGG^CC
HaeII 362 GGCGCT [GA]GCGC^[CT]
HhaI 363 GCGC GCG^C
HinP1I 363 GCGC G^CGC
HphI 971 TCACC GGTGA(8/7)
ItaI 746 GCTGC GC^[ACGT]GC
Ksp632I 7 CTCTTC CTCTTC(1/4)
MamI 428 GATTTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 867 CAATTG C^AATTG
MluNI 811 TGGCCA TGG^CCA
MscI 811 TGGCCA TGG^CCA
MspA1I 744 CAGCTG C[AC]G^C[GT]G
MunI 867 CAATTG C^AATTG
NaeI 40 GCCGGC GCC^GGC
NdeI 306 CATATG CA^TATG
NgoMIV 40 GCCGGC G^CCGGC
NlaIV 336 GGAACC GG[ACGT]^[ACGT]CC
PflMI 803 CCATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 943 ACCGGT A^CCGGT
PpuMI 920 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 942 GACCGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 744 CAGCTG CAG^CTG
SfaNI 470 GCATC GCATC(5/9)
SspI 443 AATATT AAT^ATT
TaqI 600 TCGA T^CGA
TfiI 67 GAATC G^A[AT]TC
TseI 746 GCTGC G^C[AT]GC
Tsp45I 1004 GTGAC ^GT[GC]AC
TthHB8I 600 TCGA T^CGA
Van91I 803 CCATTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 442 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwI 127 GATCC GGATC(4/5)
708 GGATC
AspHI 988 GAGCAC G[AT]GC[AT]^C
1023 GAGCAC
AvaII 549 GGACC G^G[AT]CC
921 GGTCC
BbsI 289 GAAGAC GAAGAC(2/6)
589 GAAGAC
BmyI 988 GAGCAC G[AGT]GC[ACT]^C
1023 GAGCAC
BpuAI 289 GAAGAC GAAGAC(2/6)
589 GAAGAC
BseRI 727 GAGGAG GAGGAG(10/8)
997 GAGGAG
BsgI 747 CTGCAC GTGCAG(16/14)
1014 GTGCAG
BsiHKAI 988 GAGCAC G[AT]GC[AT]^C
1023 GAGCAC
BsmFI 55 GGGAC GGGAC(10/14)
889 GTCCC
Bsp1286I 988 GAGCAC G[AGT]GC[ACT]^C
1023 GAGCAC
BsrFI 40 GCCGGC [GA]^CCGG[CT]
943 ACCGGT
BstXI 613 CCAGAGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
698 CCAGAGTGGTGG
Cfr10I 40 GCCGGC [GA]^CCGG[CT]
943 ACCGGT
EcoT14I 181 CCAAGG C^C[AT][AT]GG
584 CCTTGG
HapII 41 CCGG C^CGG
944 CCGG
HpaII 41 CCGG C^CGG
944 CCGG
MaeII 265 ACGT A^CGT
977 ACGT
MseI 557 TTAA T^TAA
830 TTAA
MslI 483 CATTGTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
972 CACCGACGTG
MspI 41 CCGG C^CGG
944 CCGG
PleI 57 GACTC GAGTC(4/5)
513 GAGTC
StyI 181 CCAAGG C^C[AT][AT]GG
584 CCTTGG
Tru9I 557 TTAA T^TAA
830 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AfaI AluI BsaJI BsiYI BslI
BsmAI BsrI BssKI BstNI BstYI
Cfr13I DdeI DpnII EaeI EcoRII
HaeIII HinfI MaeIII MboI MboII
MflI MnlI MvaI MwoI NdeII
NlaIII RsaI Sau3AI Sau96I ScrFI
Tsp509I TspRI XhoII