################################## # NO CUT ENZYMES ( 184 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BalI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BpmI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT22I FbaI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MaeIII MamI MfeI MluI MluNI MroI MscI MseI MunI MvaI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmlI SnaBI SphI Sse8387I SspBI SspI StuI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I Tth111I TthHB8I Van91I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AluI 17 AGCT AG^CT AspHI 3 GTGCTC G[AT]GC[AT]^C AvaI 103 CCCGGG C^[CT]CG[GA]G AvaII 89 GGACC G^G[AT]CC BamHI 99 GGATCC G^GATCC BbsI 160 GAAGAC GAAGAC(2/6) BfaI 355 CTAG C^TAG BlpI 5 GCTCAGC GC^T[ACGT]AGC BmyI 3 GTGCTC G[AGT]GC[ACT]^C Bpu1102I 5 GCTCAGC GC^T[ACGT]AGC BpuAI 160 GAAGAC GAAGAC(2/6) BsiHKAI 3 GTGCTC G[AT]GC[AT]^C BsoBI 103 CCCGGG C^[CT]CG[GA]G Bsp1286I 3 GTGCTC G[AGT]GC[ACT]^C BsrBI 73 GAGCGG CCGCTC(-3/-3) BsrFI 186 GCCGGT [GA]^CCGG[CT] BstYI 99 GGATCC [GA]^GATC[CT] CelII 5 GCTCAGC GC^T[ACGT]AGC Cfr10I 186 GCCGGT [GA]^CCGG[CT] Cfr13I 89 GGACC G^G[ACGT]CC DdeI 6 CTCAG C^T[ACGT]AG DpnII 100 GATC ^GATC HaeIII 337 GGCC GG^CC HgaI 61 GACGC GACGC(5/10) HinfI 163 GACTC G^A[ACGT]TC MaeI 355 CTAG C^TAG MboI 100 GATC ^GATC MboII 160 GAAGA GAAGA(8/7) MflI 99 GGATCC [GA]^GATC[CT] NdeII 100 GATC ^GATC NlaIII 252 CATG CATG^ PleI 163 GACTC GAGTC(4/5) Sau3AI 100 GATC ^GATC Sau96I 89 GGACC G^G[ACGT]CC SmaI 103 CCCGGG CCC^GGG SpeI 354 ACTAGT A^CTAGT TspRI 177 CACTG CA[GC]TG[ACGT][ACGT]^ XhoII 99 GGATCC [GA]^GATC[CT] XmaCI 103 CCCGGG C^CCGGG XmaI 103 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AlwI 100 GATCC GGATC(4/5) 99 GGATC BbvI 18 GCTGC GCAGC(8/12) 21 GCTGC BsmFI 59 GGGAC GGGAC(10/14) 88 GGGAC BsrI 178 ACTGG ACTGG(1/-1) 295 CCAGT BstF5I 153 GGATG GGATG(2/0) 266 GGATG CfoI 64 GCGC GCG^C 123 GCGC EcoT14I 333 CCTTGG C^C[AT][AT]GG 348 CCTTGG FokI 153 GGATG GGATG(9/13) 266 GGATG HhaI 64 GCGC GCG^C 123 GCGC HinP1I 64 GCGC G^CGC 123 GCGC HphI 181 GGTGA GGTGA(8/7) 226 GGTGA MnlI 52 GAGG CCTC(7/6) 79 GAGG MslI 175 CACACTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 261 CACAAGGATG MspA1I 115 CAGCGG C[AC]G^C[GT]G 130 CAGCGG StyI 333 CCTTGG C^C[AT][AT]GG 348 CCTTGG TseI 18 GCTGC G^C[AT]GC 21 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 18 enzymes) # ######################################### AccII AciI BcnI BsaJI BssKI BstUI Cac8I FauI Fnu4HI HapII HpaII ItaI MspI MvnI MwoI NciI NlaIV ScrFI