################################## # NO CUT ENZYMES ( 150 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI Alw44I AocI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AvaI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaWI BseAI BsgI BsiEI BsiWI BsoBI Bsp1407I BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstYI BstZ17I Bsu36I CelII Cfr10I CpoI DraI DraIII DrdI EagI EarI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HindIII HpaI HphI KasI KpnI Ksp632I MaeII MamI MfeI MflI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AcsI 677 AAATTT [GA]^AATT[CT] AcyI 135 GACGCC G[GA]^CG[CT]C AhdI 135 GACGCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AluI 73 AGCT AG^CT AlwI 286 GGATC GGATC(4/5) AlwNI 386 CAGTTACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 323 AGCGCT AGC^GCT ApoI 677 AAATTT [GA]^AATT[CT] AspEI 135 GACGCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvaII 599 GGACC G^G[AT]CC BanII 72 GAGCTC G[GA]GC[CT]^C BbsI 497 GAAGAC GAAGAC(2/6) BcgI 438 GCACAGAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 380 GTATCC GTATCC(6/5) BcnI 417 CCCGG CC^[GC]GG BfaI 493 CTAG C^TAG BpuAI 497 GAAGAC GAAGAC(2/6) BsaHI 135 GACGCC G[GA]^CG[CT]C BsaI 539 GAGACC GGTCTC(1/5) BseRI 741 CTCCTC GAGGAG(10/8) BsmBI 133 GAGACG CGTCTC(1/5) BsmFI 275 GTCCC GGGAC(10/14) BsmI 184 GCATTC GAATGC(1/-1) BspDI 155 ATCGAT AT^CGAT BstAPI 341 GCATGTGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 330 CCACCGAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 324 GCGC GCG^C ClaI 155 ATCGAT AT^CGAT DraII 704 AGGGCCT [GA]G^G[ACGT]CC[CT] Eam1105I 135 GACGCCTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 323 AGCGCT AGC^GCT EcoNI 213 CCTGGGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 704 AGGGCCT [GA]G^G[ACGT]CC[CT] HaeII 323 AGCGCT [GA]GCGC^[CT] HapII 418 CCGG C^CGG HgaI 135 GACGC GACGC(5/10) HhaI 324 GCGC GCG^C Hin1I 135 GACGCC G[GA]^CG[CT]C HinP1I 324 GCGC G^CGC HpaII 418 CCGG C^CGG KspI 53 CCGCGG CCGC^GG MaeI 493 CTAG C^TAG MboII 497 GAAGA GAAGA(8/7) MseI 403 TTAA T^TAA MspI 418 CCGG C^CGG NciI 417 CCCGG CC^[GC]GG PleI 755 GACTC GAGTC(4/5) SacI 72 GAGCTC GAGCT^C SacII 53 CCGCGG CCGC^GG StuI 221 AGGCCT AGG^CCT Tru9I 403 TTAA T^TAA Tsp45I 516 GTCAC ^GT[GC]AC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### BsmAI 133 GAGAC GTCTC(1/5) 539 GAGAC BstNI 139 CCTGG CC^[AT]GG 213 CCTGG DdeI 434 CTCAG C^T[ACGT]AG 509 CTAAG DsaI 53 CCGCGG C^C[GA][CT]GG 453 CCACGG EaeI 2 TGGCCG [CT]^GGCC[GA] 50 TGGCCG EcoRII 139 CCTGG ^CC[AT]GG 213 CCTGG EcoT14I 174 CCAAGG C^C[AT][AT]GG 282 CCAAGG MaeIII 388 GTTAC ^GT[ACGT]AC 516 GTCAC MslI 331 CACCGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 507 CACTAAGATG MspA1I 53 CCGCGG C[AC]G^C[GT]G 360 CCGCTG MvaI 139 CCTGG CC^[AT]GG 213 CCTGG NspI 167 GCATGT [GA]CATG^[CT] 341 GCATGT PflMI 174 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 471 CCATGACTTGG StyI 174 CCAAGG C^C[AT][AT]GG 282 CCAAGG TaqI 156 TCGA T^CGA 447 TCGA TfiI 25 GATTC G^A[AT]TC 489 GAATC Tsp509I 678 AATT ^AATT 774 AATT TspRI 237 CAGTG CA[GC]TG[ACGT][ACGT]^ 617 CAGTG TthHB8I 156 TCGA T^CGA 447 TCGA Van91I 174 CCAAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 471 CCATGACTTGG ######################################### # 3 OR MORE CUT ENZYMES ( 36 enzymes) # ######################################### AccII AciI AfaI AspHI BbvI BmyI BsaJI BsiHKAI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstUI Cac8I Cfr13I DpnII FauI Fnu4HI FokI HaeIII HinfI ItaI MboI MnlI MvnI MwoI NdeII NlaIII RsaI Sau3AI Sau96I ScrFI SfaNI TseI