Restriction map for pFN21AB9898
 (Circular) (Six-base) MAPSORT of: pFN21AB9898.seq  Check: 1567  from: 1  to: 10711



pFN21AB9898  10711 bp

 With 182 enzymes: SgfI * 

                        February 10, 2017 15:17  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:   5050    5050 
   Size:      10711
 
AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    8521     278 
   Size:         53      83     186     936    6985    2468

  Fragments arranged by size: 

                6985    2468     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    7018    8054    1937 
   Size:       5081    1036    4594

  Fragments arranged by size: 

                5081    4594    1036
 
Acc65I G'GTAC_C

Cuts at:   8032    8032 
   Size:      10711
 
AclI AA'CG_TT

Cuts at:   1563    8838    9211    1563 
   Size:       7275     373    3063

  Fragments arranged by size: 

                7275    3063     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    2309    2525    3246    3647    3758    4110    4668    5120 
   Size:        677     216     721     401     111     352     558     452

Cuts at:   5120    5250    6110    6744    7866    8774   10158   10426    1632 
   Size:        130     860     634    1122     908    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1122     908     860     721     677     634
                 558     452     401     352     268     216     130     111
            
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197   10514
 
AflIII A'CryG_T

Cuts at:   1203    3406    9610   10587    1203 
   Size:       2203    6204     977    1327

  Fragments arranged by size: 

                6204    2203    1327     977
 
AgeI A'CCGG_T

Cuts at:   9542    9542 
   Size:      10711
 
AhdI GACnn_n'nnGTC

Cuts at:   7003    9440    7003 
   Size:       2437    8274
 
AleI CACnn'nnGTG

Cuts at:   1166    5664    6073    1166 
   Size:       4498     409    5804

  Fragments arranged by size: 

                5804    4498     409
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    3098    6091    1184 
   Size:       1914    2993    5804

  Fragments arranged by size: 

                5804    2993    1914
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   6782    6814    6782 
   Size:         32   10679
 
AlwNI CAG_nnn'CTG

Cuts at:   2548    2645    4030    4133    5098    5830    6343    6671    8454 
   Size:         97    1385     103     965     732     513     328    1783

Cuts at:   8454   10026    2548 
   Size:       1572    3233

  Fragments arranged by size: 

                3233    1783    1572    1385     965     732     513     328
                 103      97
 
ApaLI G'TGCA_C

Cuts at:   3983    8768    9924    3983 
   Size:       4785    1156    4770

  Fragments arranged by size: 

                4785    4770    1156
 
ApoI r'AATT_y

Cuts at:   1493    3056    3826    4450    5356    6218    6832    8020    8246 
   Size:       1563     770     624     906     862     614    1188     226

Cuts at:   8246    8398   10507    1493 
   Size:        152    2109    1697

  Fragments arranged by size: 

                2109    1697    1563    1188     906     862     770     624
                 614     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    9265     160 
   Size:        872    8233    1606

  Fragments arranged by size: 

                8233    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      10711
 
AvaI C'yCGr_G

Cuts at:   1870    1943    7980    8036    1870 
   Size:         73    6037      56    4545

  Fragments arranged by size: 

                6037    4545      73      56
 
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'

Cuts at:   3887    3920    5358    5391    3887 
   Size:         33    1438      33    9207

  Fragments arranged by size: 

                9207    1438      33      33
 
BamHI G'GATC_C

Cuts at:   5833    6695    7570    8041    8408    5833 
   Size:        862     875     471     367    8136

  Fragments arranged by size: 

                8136     875     862     471     367
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    3373    5849    6004    6329    8032 
   Size:        325     217     627    1586    2476     155     325    1703

Cuts at:   8032    9487     618 
   Size:       1455    1842

  Fragments arranged by size: 

                2476    1842    1703    1586    1455     627     325     325
                 217     155
 
BanII G_rGCy'C

Cuts at:   1391    2137    2322    3849    7890    8030    1391 
   Size:        746     185    1527    4041     140    4072

  Fragments arranged by size: 

                4072    4041    1527     746     185     140
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899    6213     928 
   Size:        971    4314    5426

  Fragments arranged by size: 

                5426    4314     971
 
BbvCI CC'TCA_GC

Cuts at:   3252    5759    3252 
   Size:       2507    8204
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    8902    8936    1903 
   Size:         34    6965      34    3678

  Fragments arranged by size: 

                6965    3678      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   5359    6996    8603    9813    5359 
   Size:       1637    1607    1210    6257

  Fragments arranged by size: 

                6257    1637    1607    1210
 
BclI T'GATC_A

Cuts at:   1548    4306    1548 
   Size:       2758    7953
 
BfrBI ATG'CAT

Cuts at:   6035    6035 
   Size:      10711
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3844    7766    9322 
   Size:        107     122      71     631     605    2172    3922    1556

Cuts at:   9322     136 
   Size:       1525

  Fragments arranged by size: 

                3922    2172    1556    1525     631     605     122     107
                  71
 
BglII A'GATC_T

Cuts at:   3655    4633   10706    3655 
   Size:        978    6073    3660

  Fragments arranged by size: 

                6073    3660     978
 
BlpI GC'TnA_GC

Cuts at:   4749    7866    8128    4749 
   Size:       3117     262    7332

  Fragments arranged by size: 

                7332    3117     262
 
Bme1580I G_kGCm'C

Cuts at:   1790    3666    3987    8772    9928    1790 
   Size:       1876     321    4785    1156    2573

  Fragments arranged by size: 

                4785    2573    1876    1156     321
 
BmgBI CAC'GTC

Cuts at:   1318    6280    1318 
   Size:       4962    5749
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    4088    6952    8442 
   Size:        416      53      83     399      24    2671    2864    1490

Cuts at:   8442    9400     442 
   Size:        958    1753

  Fragments arranged by size: 

                2864    2671    1753    1490     958     416     399      83
                  53      24
 
BmtI G_CTAG'C

Cuts at:   1056    3044    1056 
   Size:       1988    8723
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   6475    6475 
   Size:      10711
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    2090    3905    3941    4259    4442    5990    6470    9371 
   Size:        851    1815      36     318     183    1548     480    2901

Cuts at:   9371    1239 
   Size:       2579

  Fragments arranged by size: 

                2901    2579    1815    1548     851     480     318     183
                  36
 
Bpu10I CC'TnA_GC

Cuts at:   3252    5759    6306    3252 
   Size:       2507     547    7657

  Fragments arranged by size: 

                7657    2507     547
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   3524    6795    8836    9701    9999    3524 
   Size:       3271    2041     865     298    4236

  Fragments arranged by size: 

                4236    3271    2041     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    5075    6625    9374     882 
   Size:        617    3576    1550    2749    2219

  Fragments arranged by size: 

                3576    2749    2219    1550     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    6497    8502     493 
   Size:        711    5293    2005    2702

  Fragments arranged by size: 

                5293    2702    2005     711
 
BsaBI GATnn'nnATC

Cuts at:   5690   10705    5690 
   Size:       5015    5696
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    8518    8900    9535     275 
   Size:         53      83     186     936    6985     382     635    1451

  Fragments arranged by size: 

                6985    1451     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   9144    9542    9816    9963   10374    9144 
   Size:        398     274     147     411    9481

  Fragments arranged by size: 

                9481     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    3878    3908    4655    4685    6914 
   Size:         30     165      30    2475      30     747      30    2229

Cuts at:   6914    6944    7578    7608    7948    7978    1178 
   Size:         30     634      30     340      30    3911

  Fragments arranged by size: 

                3911    2475    2229     747     634     340     165      30
                  30      30      30      30      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    3222    3225    6326    1186 
   Size:       2036       3    3101    5571

  Fragments arranged by size: 

                5571    3101    2036       3
 
BseYI C'CCAG_C

Cuts at:   5239    6579    6588    6618    6636    9914   10410    5239 
   Size:       1340       9      30      18    3278     496    5540

  Fragments arranged by size: 

                5540    3278    1340     496      30      18       9
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    2750    4043    5081    7326    7542    1163 
   Size:        573    1014    1293    1038    2245     216    4332

  Fragments arranged by size: 

                4332    2245    1293    1038    1014     573     216
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    8161    8922    9950    1540 
   Size:        275     186    6160     761    1028    2301

  Fragments arranged by size: 

                6160    2301    1028     761     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   2137    3891    3987    6070    6142    8772    8857    9928    2137 
   Size:       1754      96    2083      72    2630      85    1071    2920

  Fragments arranged by size: 

                2920    2630    2083    1754    1071      96      85      72
            
 
BsmI GAATG_Cn'

Cuts at:   8222    8315    8222 
   Size:         93   10618
 
BsmBI CGTCTCn'nnnn_

Cuts at:   5747    6709    5747 
   Size:        962    9749
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2137    2322    3666    3849    3891    3987    6070 
   Size:        399     347     185    1344     183      42      96    2083

Cuts at:   6070    6142    7890    8030    8772    8857    9928    1391 
   Size:         72    1748     140     742      85    1071    2174

  Fragments arranged by size: 

                2174    2083    1748    1344    1071     742     399     347
                 185     183     140      96      85      72      42
 
BspEI T'CCGG_A

Cuts at:  10374   10374 
   Size:      10711
 
BspHI T'CATG_A

Cuts at:   7183    8600   10330    7183 
   Size:       1417    1730    7564

  Fragments arranged by size: 

                7564    1730    1417
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    5050    5453    6809    7905    8066     844 
   Size:       4206     403    1356    1096     161    3489

  Fragments arranged by size: 

                4206    3489    1356    1096     403     161
 
BsrBI CCG'CTC

Cuts at:   1394    1823    8598    1394 
   Size:        429    6775    3507

  Fragments arranged by size: 

                6775    3507     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    2721    2937    6910    9206    9380      65 
   Size:       1183    1473     216    3973    2296     174    1396

  Fragments arranged by size: 

                3973    2296    1473    1396    1183     216     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    5787    9355    9542    1700 
   Size:        110    3977    3568     187    2869

  Fragments arranged by size: 

                3977    3568    2869     187     110
 
BsrGI T'GTAC_A

Cuts at:     96    7147      96 
   Size:       7051    3660
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:      10711
 
BssSI C'ACGA_G

Cuts at:   3651    8771    9783    3651 
   Size:       5120    1012    4579

  Fragments arranged by size: 

                5120    4579    1012
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794    4597    6032    6166     794 
   Size:       3803    1435     134    5339

  Fragments arranged by size: 

                5339    3803    1435     134
 
BstBI TT'CG_AA

Cuts at:   9515    9515 
   Size:      10711
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:      10711
 
BstXI CCAn_nnnn'nTGG

Cuts at:   6001    6001 
   Size:      10711
 
BstYI r'GATC_y

Cuts at:   1972    3655    4633    5833    5916    6695    6973    7570    7692 
   Size:       1683     978    1200      83     779     278     597     122

Cuts at:   7692    8041    8408    8793    8810   10262   10348   10360   10706 
   Size:        349     367     385      17    1452      86      12     346

Cuts at:  10706    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1683    1452    1200     978     779     597     385
                 367     349     346     278     122      86      83      17
                  12
 
Bsu36I CC'TnA_GG

Cuts at:   2815    3369    6234    2815 
   Size:        554    2865    7292

  Fragments arranged by size: 

                7292    2865     554
 
BtgI C'CryG_G

Cuts at:    513    1065    2004    2655    5085    7593    7837     513 
   Size:        552     939     651    2430    2508     244    3387

  Fragments arranged by size: 

                3387    2508    2430     939     651     552     244
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226    2790     487 
   Size:        683      56    1564    8408

  Fragments arranged by size: 

                8408    1564     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2120    2524    2622    2880    3574    3758    5211    5623    5682 
   Size:        404      98     258     694     184    1453     412      59

Cuts at:   5682    6117    8227    9021    9041    2120 
   Size:        435    2110     794      20    3790

  Fragments arranged by size: 

                3790    2110    1453     794     694     435     412     404
                 258     184      98      59      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:      10711
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    4932    4967     529 
   Size:         35    4368      35    6273

  Fragments arranged by size: 

                6273    4368      35      35
 
DraI TTT'AAA

Cuts at:   3117    7662    8015    3117 
   Size:       4545     353    5813

  Fragments arranged by size: 

                5813    4545     353
 
DraIII CAC_nnn'GTG

Cuts at:   2163    2672    3982    4034    2163 
   Size:        509    1310      52    8840

  Fragments arranged by size: 

                8840    1310     509      52
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    9718     809 
   Size:        507    8402    1802

  Fragments arranged by size: 

                8402    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    4333    5656    7195 
   Size:         54    1425      50     275      18    2503    1323    1539

Cuts at:   7195    7590    8158    9047   10676       8 
   Size:        395     568     889    1629      43

  Fragments arranged by size: 

                2503    1629    1539    1425    1323     889     568     395
                 275      54      50      43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    8158    1537 
   Size:        275    6346    4090

  Fragments arranged by size: 

                6346    4090     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2656    3243    5616    5799    5955    6754    7338    7788 
   Size:       1303     587    2373     183     156     799     584     450

Cuts at:   7788    8641    1353 
   Size:        853    3423

  Fragments arranged by size: 

                3423    2373    1303     853     799     587     584     450
                 183     156
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    9298    9672    9818     387 
   Size:       1064     138    7709     374     146    1280

  Fragments arranged by size: 

                7709    1280    1064     374     146     138
 
EcoICRI GAG'CTC

Cuts at:   2135    2135 
   Size:      10711
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    2090    2309    2525    3246    3647    3758    3905 
   Size:        393     458     219     216     721     401     111     147

Cuts at:   3905    3941    4110    4259    4442    4668    5120    5250    5990 
   Size:         36     169     149     183     226     452     130     740

Cuts at:   5990    6110    6470    6744    7866    8774    9371   10158   10426 
   Size:        120     360     274    1122     908     597     787     268

Cuts at:  10426    1239 
   Size:       1524

  Fragments arranged by size: 

                1524    1122     908     787     740     721     597     458
                 452     401     393     360     274     268     226     219
                 216     183     169     149     147     130     120     111
                  36
 
EcoNI CCTnn'n_nnAGG

Cuts at:   3264    3264 
   Size:      10711
 
EcoO109I rG'GnC_Cy

Cuts at:   3709    5438    5465    7991    3709 
   Size:       1729      27    2526    6429

  Fragments arranged by size: 

                6429    2526    1729      27
 
EcoRI G'AATT_C

Cuts at:   3056    6832    8020    8398    3056 
   Size:       3776    1188     378    5369

  Fragments arranged by size: 

                5369    3776    1188     378
 
EcoRV GAT'ATC

Cuts at:   6766    6766 
   Size:      10711
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:   3193    3876    4655    3193 
   Size:        683     779    9249

  Fragments arranged by size: 

                9249     779     683
 
FspI TGC'GCA

Cuts at:   4740    9217    4740 
   Size:       4477    6234
 
HaeII r_GCGC'y

Cuts at:   1678    9858    1678 
   Size:       8180    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    2074    2106    2122    2154    3379 
   Size:         32     135      32     502      32      16      32    1225

Cuts at:   3379    3411    3878    3910    4198    4230    4270    4302    4537 
   Size:         32     467      32     288      32      40      32     235

Cuts at:   4537    4569    4655    4687    5381    5413    6914    6946    6989 
   Size:         32      86      32     694      32    1501      32      43

Cuts at:   6989    7021    7529    7561    9352    9384    9426    9458    1373 
   Size:         32     508      32    1791      32      42      32    2626

  Fragments arranged by size: 

                2626    1791    1501    1225     694     508     502     467
                 288     235     135      86      43      42      40      32
                  32      32      32      32      32      32      32      32
                  32      32      32      32      32      32      32      16
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2417    4730    6676    8055    8301     669 
   Size:        988     281     479    2313    1946    1379     246    3079

  Fragments arranged by size: 

                3079    2313    1946    1379     988     479     281     246
            
 
HindIII A'AGCT_T

Cuts at:    748    3858    4680    6437     748 
   Size:       3110     822    1757    5022

  Fragments arranged by size: 

                5022    3110    1757     822
 
HpaI GTT'AAC

Cuts at:   8301    8301 
   Size:      10711
 
KpnI G_GTAC'C

Cuts at:   8036    8036 
   Size:      10711
 
MfeI C'AATT_G

Cuts at:   3212    8310    3212 
   Size:       5098    5613
 
MluI A'CGCG_T

Cuts at:  10587   10587 
   Size:      10711
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    3855    7625    9825   10009    1305 
   Size:       2550    3770    2200     184    2007

  Fragments arranged by size: 

                3770    2550    2200    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    4335    5658    7197      10 
   Size:         54    1425     343    2503    1323    1539    3524

  Fragments arranged by size: 

                3524    2503    1539    1425    1323     343      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2667    2726    2751    3409    4773    4779    5664 
   Size:        648    1501      59      25     658    1364       6     885

Cuts at:   5664    5696    5999    6073    6187    6898    7934    8669    9028 
   Size:         32     303      74     114     711    1036     735     359

Cuts at:   9028    9187     518 
   Size:        159    2042

  Fragments arranged by size: 

                2042    1501    1364    1036     885     735     711     658
                 648     359     303     159     114      74      59      32
                  25       6
 
MspA1I CmG'CkG

Cuts at:   1606    2870    3883    5294    5716    6431    8127    8804    9952 
   Size:       1264    1013    1411     422     715    1696     677    1148

Cuts at:   9952   10197    1606 
   Size:        245    2120

  Fragments arranged by size: 

                2120    1696    1411    1264    1148    1013     715     677
                 422     245
 
NcoI C'CATG_G

Cuts at:    513    1065    2004    5085    7837     513 
   Size:        552     939    3081    2752    3387

  Fragments arranged by size: 

                3387    3081    2752     939     552
 
NdeI CA'TA_TG

Cuts at:    387    7969     387 
   Size:       7582    3129
 
NheI G'CTAG_C

Cuts at:   1052    3040    1052 
   Size:       1988    8723
 
NotI GC'GGCC_GC

Cuts at:   8158    8158 
   Size:      10711
 
NsiI A_TGCA'T

Cuts at:   6037    6037 
   Size:      10711
 
NspI r_CATG'y

Cuts at:   1132    1229    3410    3838    4488    4600    4853    4976    5884 
   Size:         97    2181     428     650     112     253     123     908

Cuts at:   5884    6126    6169    7321    7372    7649    8069    9614    1132 
   Size:        242      43    1152      51     277     420    1545    2229

  Fragments arranged by size: 

                2229    2181    1545    1152     908     650     428     420
                 277     253     242     123     112      97      51      43
            
 
PasI CC'CwG_GG

Cuts at:   1348    6860    1348 
   Size:       5512    5199
 
PciI A'CATG_T

Cuts at:   3406    9610    3406 
   Size:       6204    4507
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    3560    7052    7204    7510    7607    7739    1108 
   Size:       2452    3492     152     306      97     132    4080

  Fragments arranged by size: 

                4080    3492    2452     306     152     132      97
 
PfoI T'CCnGG_A

Cuts at:   2679    2679 
   Size:      10711
 
PmeI GTTT'AAAC

Cuts at:   8015    8015 
   Size:      10711
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   2295    2327    8780    8812   10293   10325    2295 
   Size:         32    6453      32    1481      32    2681

  Fragments arranged by size: 

                6453    2681    1481      32      32      32
 
PpuMI rG'GwC_Cy

Cuts at:   5438    5465    5438 
   Size:         27   10684
 
PshAI GACnn'nnGTC

Cuts at:   1613    3526    1613 
   Size:       1913    8798
 
PsiI TTA'TAA

Cuts at:   8281    8281 
   Size:      10711
 
PstI C_TGCA'G

Cuts at:    830    2519    2875    3013    3484    5221    5677    6430    6820 
   Size:       1689     356     138     471    1737     456     753     390

Cuts at:   6820    8063     830 
   Size:       1243    3478

  Fragments arranged by size: 

                3478    1737    1689    1243     753     471     456     390
                 356     138
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:      10711
 
PvuII CAG'CTG

Cuts at:   2870    3883    5294    6431    2870 
   Size:       1013    1411    1137    7150

  Fragments arranged by size: 

                7150    1411    1137    1013
 
SacI G_AGCT'C

Cuts at:   2137    2137 
   Size:      10711
 
SalI G'TCGA_C

Cuts at:   1936    8053    1936 
   Size:       6117    4594
 
SbfI CC_TGCA'GG

Cuts at:   8063    8063 
   Size:      10711
 
ScaI AGT'ACT

Cuts at:   8959   10671    8959 
   Size:       1712    8999
 
SexAI A'CCwGG_T

Cuts at:   3978    3978 
   Size:      10711
 
SfcI C'TryA_G

Cuts at:    826    1045    2463    2515    2637    2871    3009    3480    3578 
   Size:        219    1418      52     122     234     138     471      98

Cuts at:   3578    4691    5217    5673    6426    6596    6816    7300    8059 
   Size:       1113     526     456     753     170     220     484     759

Cuts at:   8059    9194    9875   10066     826 
   Size:       1135     681     191    1471

  Fragments arranged by size: 

                1471    1418    1135    1113     759     753     681     526
                 484     471     456     234     220     219     191     170
                 138     122      98      52
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:      10711
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    5787    1700 
   Size:       4087    6624
 
SmaI CCC'GGG

Cuts at:   1872    8038    1872 
   Size:       6166    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    3539    6810    8815    9716    9978     820 
   Size:        197     926    1596    3271    2005     901     262    1553

  Fragments arranged by size: 

                3271    2005    1596    1553     926     901     262     197
            
 
SnaBI TAC'GTA

Cuts at:    493    8502     493 
   Size:       8009    2702
 
SpeI A'CTAG_T

Cuts at:    152    7498     152 
   Size:       7346    3365
 
SphI G_CATG'C

Cuts at:   1132    4488    4853    7649    8069    1132 
   Size:       3356     365    2796     420    3774

  Fragments arranged by size: 

                3774    3356    2796     420     365
 
SspI AAT'ATT

Cuts at:      5      52    2702    4604    8635       5 
   Size:         47    2650    1902    4031    2081

  Fragments arranged by size: 

                4031    2650    2081    1902      47
 
StuI AGG'CCT

Cuts at:   1517    2888    3442    1517 
   Size:       1371     554    8786

  Fragments arranged by size: 

                8786    1371     554
 
StyI C'CwwG_G

Cuts at:    513    1065    2004    3851    3934    5085    5921    7046    7441 
   Size:        552     939    1847      83    1151     836    1125     395

Cuts at:   7441    7837    8150     513 
   Size:        396     313    3074

  Fragments arranged by size: 

                3074    1847    1151    1125     939     836     552     396
                 395     313      83
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2817    3881    5587    7039    7243    8738    8755 
   Size:       1255    1237    1064    1706    1452     204    1495      17

Cuts at:   8755    8908    9093    9538     325 
   Size:        153     185     445    1498

  Fragments arranged by size: 

                1706    1498    1495    1452    1255    1237    1064     445
                 204     185     153      17
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    4429    5883    7147 
   Size:        274      80      33      51    1443    2452    1454    1264

Cuts at:   7147    8957   10669      96 
   Size:       1810    1712     138

  Fragments arranged by size: 

                2452    1810    1712    1454    1443    1264     274     138
                  80      51      33
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   7578    7610   10671   10703    7578 
   Size:         32    3061      32    7586

  Fragments arranged by size: 

                7586    3061      32      32
 
Tth111I GACn'n_nGTC

Cuts at:   1316    2046    6667    7930    1316 
   Size:        730    4621    1263    4097

  Fragments arranged by size: 

                4621    4097    1263     730
 
XbaI T'CTAG_A

Cuts at:   7217    8047    7217 
   Size:        830    9881
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2357    4721    7049    7186    7385    2357 
   Size:       2364    2328     137     199    5683

  Fragments arranged by size: 

                5683    2364    2328     199     137
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:      10711
 
XmaI C'CCGG_G

Cuts at:   1870    8036    1870 
   Size:       6166    4545
 
XmnI GAAnn'nnTTC

Cuts at:   4356    8840    4356 
   Size:       4484    6227
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    8519     276 
   Size:         53      83     186     936    6985    2468

  Fragments arranged by size: 

                6985    2468     936     186      83      53
 

 Enzymes that do cut:

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI    ApaLI     ApoI
     AseI    AsiSI     AvaI     BaeI    BamHI     BanI    BanII     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiEI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI     MfeI
     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI     NheI
     NotI     NsiI     NspI     PasI     PciI    PflMI     PfoI     PmeI
     PpiI    PpuMI    PshAI     PsiI     PstI     PvuI    PvuII     SacI
     SalI     SbfI     ScaI    SexAI     SfcI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SspI     StuI     StyI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI

 Enzymes that do not cut: 

     AfeI     ApaI     AscI    AvrII     BbeI    BsiWI  BstZ17I     FseI
    FspAI     KasI     NaeI     NarI   NgoMIV     NruI     PacI     PmlI
   PspOMI    PspXI     PsrI    RsrII    SacII    SanDI     SapI     SfiI
     SfoI     SrfI     SwaI
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