(Circular) (Six-base) MAPSORT of: pFN21AB9898.seq Check: 1567 from: 1 to: 10711 pFN21AB9898 10711 bp With 182 enzymes: SgfI * February 10, 2017 15:17 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5050 5050 Size: 10711 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 8521 278 Size: 53 83 186 936 6985 2468 Fragments arranged by size: 6985 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 7018 8054 1937 Size: 5081 1036 4594 Fragments arranged by size: 5081 4594 1036 Acc65I G'GTAC_C Cuts at: 8032 8032 Size: 10711 AclI AA'CG_TT Cuts at: 1563 8838 9211 1563 Size: 7275 373 3063 Fragments arranged by size: 7275 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2309 2525 3246 3647 3758 4110 4668 5120 Size: 677 216 721 401 111 352 558 452 Cuts at: 5120 5250 6110 6744 7866 8774 10158 10426 1632 Size: 130 860 634 1122 908 1384 268 1917 Fragments arranged by size: 1917 1384 1122 908 860 721 677 634 558 452 401 352 268 216 130 111 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 10514 AflIII A'CryG_T Cuts at: 1203 3406 9610 10587 1203 Size: 2203 6204 977 1327 Fragments arranged by size: 6204 2203 1327 977 AgeI A'CCGG_T Cuts at: 9542 9542 Size: 10711 AhdI GACnn_n'nnGTC Cuts at: 7003 9440 7003 Size: 2437 8274 AleI CACnn'nnGTG Cuts at: 1166 5664 6073 1166 Size: 4498 409 5804 Fragments arranged by size: 5804 4498 409 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3098 6091 1184 Size: 1914 2993 5804 Fragments arranged by size: 5804 2993 1914 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6782 6814 6782 Size: 32 10679 AlwNI CAG_nnn'CTG Cuts at: 2548 2645 4030 4133 5098 5830 6343 6671 8454 Size: 97 1385 103 965 732 513 328 1783 Cuts at: 8454 10026 2548 Size: 1572 3233 Fragments arranged by size: 3233 1783 1572 1385 965 732 513 328 103 97 ApaLI G'TGCA_C Cuts at: 3983 8768 9924 3983 Size: 4785 1156 4770 Fragments arranged by size: 4785 4770 1156 ApoI r'AATT_y Cuts at: 1493 3056 3826 4450 5356 6218 6832 8020 8246 Size: 1563 770 624 906 862 614 1188 226 Cuts at: 8246 8398 10507 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1563 1188 906 862 770 624 614 226 152 AseI AT'TA_AT Cuts at: 160 1032 9265 160 Size: 872 8233 1606 Fragments arranged by size: 8233 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 10711 AvaI C'yCGr_G Cuts at: 1870 1943 7980 8036 1870 Size: 73 6037 56 4545 Fragments arranged by size: 6037 4545 73 56 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3887 3920 5358 5391 3887 Size: 33 1438 33 9207 Fragments arranged by size: 9207 1438 33 33 BamHI G'GATC_C Cuts at: 5833 6695 7570 8041 8408 5833 Size: 862 875 471 367 8136 Fragments arranged by size: 8136 875 862 471 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3373 5849 6004 6329 8032 Size: 325 217 627 1586 2476 155 325 1703 Cuts at: 8032 9487 618 Size: 1455 1842 Fragments arranged by size: 2476 1842 1703 1586 1455 627 325 325 217 155 BanII G_rGCy'C Cuts at: 1391 2137 2322 3849 7890 8030 1391 Size: 746 185 1527 4041 140 4072 Fragments arranged by size: 4072 4041 1527 746 185 140 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 6213 928 Size: 971 4314 5426 Fragments arranged by size: 5426 4314 971 BbvCI CC'TCA_GC Cuts at: 3252 5759 3252 Size: 2507 8204 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 8902 8936 1903 Size: 34 6965 34 3678 Fragments arranged by size: 6965 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5359 6996 8603 9813 5359 Size: 1637 1607 1210 6257 Fragments arranged by size: 6257 1637 1607 1210 BclI T'GATC_A Cuts at: 1548 4306 1548 Size: 2758 7953 BfrBI ATG'CAT Cuts at: 6035 6035 Size: 10711 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3844 7766 9322 Size: 107 122 71 631 605 2172 3922 1556 Cuts at: 9322 136 Size: 1525 Fragments arranged by size: 3922 2172 1556 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3655 4633 10706 3655 Size: 978 6073 3660 Fragments arranged by size: 6073 3660 978 BlpI GC'TnA_GC Cuts at: 4749 7866 8128 4749 Size: 3117 262 7332 Fragments arranged by size: 7332 3117 262 Bme1580I G_kGCm'C Cuts at: 1790 3666 3987 8772 9928 1790 Size: 1876 321 4785 1156 2573 Fragments arranged by size: 4785 2573 1876 1156 321 BmgBI CAC'GTC Cuts at: 1318 6280 1318 Size: 4962 5749 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4088 6952 8442 Size: 416 53 83 399 24 2671 2864 1490 Cuts at: 8442 9400 442 Size: 958 1753 Fragments arranged by size: 2864 2671 1753 1490 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3044 1056 Size: 1988 8723 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6475 6475 Size: 10711 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2090 3905 3941 4259 4442 5990 6470 9371 Size: 851 1815 36 318 183 1548 480 2901 Cuts at: 9371 1239 Size: 2579 Fragments arranged by size: 2901 2579 1815 1548 851 480 318 183 36 Bpu10I CC'TnA_GC Cuts at: 3252 5759 6306 3252 Size: 2507 547 7657 Fragments arranged by size: 7657 2507 547 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3524 6795 8836 9701 9999 3524 Size: 3271 2041 865 298 4236 Fragments arranged by size: 4236 3271 2041 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5075 6625 9374 882 Size: 617 3576 1550 2749 2219 Fragments arranged by size: 3576 2749 2219 1550 617 BsaAI yAC'GTr Cuts at: 493 1204 6497 8502 493 Size: 711 5293 2005 2702 Fragments arranged by size: 5293 2702 2005 711 BsaBI GATnn'nnATC Cuts at: 5690 10705 5690 Size: 5015 5696 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 8518 8900 9535 275 Size: 53 83 186 936 6985 382 635 1451 Fragments arranged by size: 6985 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 9144 9542 9816 9963 10374 9144 Size: 398 274 147 411 9481 Fragments arranged by size: 9481 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3878 3908 4655 4685 6914 Size: 30 165 30 2475 30 747 30 2229 Cuts at: 6914 6944 7578 7608 7948 7978 1178 Size: 30 634 30 340 30 3911 Fragments arranged by size: 3911 2475 2229 747 634 340 165 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3222 3225 6326 1186 Size: 2036 3 3101 5571 Fragments arranged by size: 5571 3101 2036 3 BseYI C'CCAG_C Cuts at: 5239 6579 6588 6618 6636 9914 10410 5239 Size: 1340 9 30 18 3278 496 5540 Fragments arranged by size: 5540 3278 1340 496 30 18 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2750 4043 5081 7326 7542 1163 Size: 573 1014 1293 1038 2245 216 4332 Fragments arranged by size: 4332 2245 1293 1038 1014 573 216 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 8161 8922 9950 1540 Size: 275 186 6160 761 1028 2301 Fragments arranged by size: 6160 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2137 3891 3987 6070 6142 8772 8857 9928 2137 Size: 1754 96 2083 72 2630 85 1071 2920 Fragments arranged by size: 2920 2630 2083 1754 1071 96 85 72 BsmI GAATG_Cn' Cuts at: 8222 8315 8222 Size: 93 10618 BsmBI CGTCTCn'nnnn_ Cuts at: 5747 6709 5747 Size: 962 9749 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2137 2322 3666 3849 3891 3987 6070 Size: 399 347 185 1344 183 42 96 2083 Cuts at: 6070 6142 7890 8030 8772 8857 9928 1391 Size: 72 1748 140 742 85 1071 2174 Fragments arranged by size: 2174 2083 1748 1344 1071 742 399 347 185 183 140 96 85 72 42 BspEI T'CCGG_A Cuts at: 10374 10374 Size: 10711 BspHI T'CATG_A Cuts at: 7183 8600 10330 7183 Size: 1417 1730 7564 Fragments arranged by size: 7564 1730 1417 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 5050 5453 6809 7905 8066 844 Size: 4206 403 1356 1096 161 3489 Fragments arranged by size: 4206 3489 1356 1096 403 161 BsrBI CCG'CTC Cuts at: 1394 1823 8598 1394 Size: 429 6775 3507 Fragments arranged by size: 6775 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2721 2937 6910 9206 9380 65 Size: 1183 1473 216 3973 2296 174 1396 Fragments arranged by size: 3973 2296 1473 1396 1183 216 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5787 9355 9542 1700 Size: 110 3977 3568 187 2869 Fragments arranged by size: 3977 3568 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 7147 96 Size: 7051 3660 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 10711 BssSI C'ACGA_G Cuts at: 3651 8771 9783 3651 Size: 5120 1012 4579 Fragments arranged by size: 5120 4579 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 4597 6032 6166 794 Size: 3803 1435 134 5339 Fragments arranged by size: 5339 3803 1435 134 BstBI TT'CG_AA Cuts at: 9515 9515 Size: 10711 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 10711 BstXI CCAn_nnnn'nTGG Cuts at: 6001 6001 Size: 10711 BstYI r'GATC_y Cuts at: 1972 3655 4633 5833 5916 6695 6973 7570 7692 Size: 1683 978 1200 83 779 278 597 122 Cuts at: 7692 8041 8408 8793 8810 10262 10348 10360 10706 Size: 349 367 385 17 1452 86 12 346 Cuts at: 10706 1972 Size: 1977 Fragments arranged by size: 1977 1683 1452 1200 978 779 597 385 367 349 346 278 122 86 83 17 12 Bsu36I CC'TnA_GG Cuts at: 2815 3369 6234 2815 Size: 554 2865 7292 Fragments arranged by size: 7292 2865 554 BtgI C'CryG_G Cuts at: 513 1065 2004 2655 5085 7593 7837 513 Size: 552 939 651 2430 2508 244 3387 Fragments arranged by size: 3387 2508 2430 939 651 552 244 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2790 487 Size: 683 56 1564 8408 Fragments arranged by size: 8408 1564 683 56 BtsI GCAGTG_nn' Cuts at: 2120 2524 2622 2880 3574 3758 5211 5623 5682 Size: 404 98 258 694 184 1453 412 59 Cuts at: 5682 6117 8227 9021 9041 2120 Size: 435 2110 794 20 3790 Fragments arranged by size: 3790 2110 1453 794 694 435 412 404 258 184 98 59 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 10711 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4932 4967 529 Size: 35 4368 35 6273 Fragments arranged by size: 6273 4368 35 35 DraI TTT'AAA Cuts at: 3117 7662 8015 3117 Size: 4545 353 5813 Fragments arranged by size: 5813 4545 353 DraIII CAC_nnn'GTG Cuts at: 2163 2672 3982 4034 2163 Size: 509 1310 52 8840 Fragments arranged by size: 8840 1310 509 52 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 9718 809 Size: 507 8402 1802 Fragments arranged by size: 8402 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4333 5656 7195 Size: 54 1425 50 275 18 2503 1323 1539 Cuts at: 7195 7590 8158 9047 10676 8 Size: 395 568 889 1629 43 Fragments arranged by size: 2503 1629 1539 1425 1323 889 568 395 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 8158 1537 Size: 275 6346 4090 Fragments arranged by size: 6346 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2656 3243 5616 5799 5955 6754 7338 7788 Size: 1303 587 2373 183 156 799 584 450 Cuts at: 7788 8641 1353 Size: 853 3423 Fragments arranged by size: 3423 2373 1303 853 799 587 584 450 183 156 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 9298 9672 9818 387 Size: 1064 138 7709 374 146 1280 Fragments arranged by size: 7709 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2135 2135 Size: 10711 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2090 2309 2525 3246 3647 3758 3905 Size: 393 458 219 216 721 401 111 147 Cuts at: 3905 3941 4110 4259 4442 4668 5120 5250 5990 Size: 36 169 149 183 226 452 130 740 Cuts at: 5990 6110 6470 6744 7866 8774 9371 10158 10426 Size: 120 360 274 1122 908 597 787 268 Cuts at: 10426 1239 Size: 1524 Fragments arranged by size: 1524 1122 908 787 740 721 597 458 452 401 393 360 274 268 226 219 216 183 169 149 147 130 120 111 36 EcoNI CCTnn'n_nnAGG Cuts at: 3264 3264 Size: 10711 EcoO109I rG'GnC_Cy Cuts at: 3709 5438 5465 7991 3709 Size: 1729 27 2526 6429 Fragments arranged by size: 6429 2526 1729 27 EcoRI G'AATT_C Cuts at: 3056 6832 8020 8398 3056 Size: 3776 1188 378 5369 Fragments arranged by size: 5369 3776 1188 378 EcoRV GAT'ATC Cuts at: 6766 6766 Size: 10711 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3193 3876 4655 3193 Size: 683 779 9249 Fragments arranged by size: 9249 779 683 FspI TGC'GCA Cuts at: 4740 9217 4740 Size: 4477 6234 HaeII r_GCGC'y Cuts at: 1678 9858 1678 Size: 8180 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2074 2106 2122 2154 3379 Size: 32 135 32 502 32 16 32 1225 Cuts at: 3379 3411 3878 3910 4198 4230 4270 4302 4537 Size: 32 467 32 288 32 40 32 235 Cuts at: 4537 4569 4655 4687 5381 5413 6914 6946 6989 Size: 32 86 32 694 32 1501 32 43 Cuts at: 6989 7021 7529 7561 9352 9384 9426 9458 1373 Size: 32 508 32 1791 32 42 32 2626 Fragments arranged by size: 2626 1791 1501 1225 694 508 502 467 288 235 135 86 43 42 40 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 2417 4730 6676 8055 8301 669 Size: 988 281 479 2313 1946 1379 246 3079 Fragments arranged by size: 3079 2313 1946 1379 988 479 281 246 HindIII A'AGCT_T Cuts at: 748 3858 4680 6437 748 Size: 3110 822 1757 5022 Fragments arranged by size: 5022 3110 1757 822 HpaI GTT'AAC Cuts at: 8301 8301 Size: 10711 KpnI G_GTAC'C Cuts at: 8036 8036 Size: 10711 MfeI C'AATT_G Cuts at: 3212 8310 3212 Size: 5098 5613 MluI A'CGCG_T Cuts at: 10587 10587 Size: 10711 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3855 7625 9825 10009 1305 Size: 2550 3770 2200 184 2007 Fragments arranged by size: 3770 2550 2200 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4335 5658 7197 10 Size: 54 1425 343 2503 1323 1539 3524 Fragments arranged by size: 3524 2503 1539 1425 1323 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2667 2726 2751 3409 4773 4779 5664 Size: 648 1501 59 25 658 1364 6 885 Cuts at: 5664 5696 5999 6073 6187 6898 7934 8669 9028 Size: 32 303 74 114 711 1036 735 359 Cuts at: 9028 9187 518 Size: 159 2042 Fragments arranged by size: 2042 1501 1364 1036 885 735 711 658 648 359 303 159 114 74 59 32 25 6 MspA1I CmG'CkG Cuts at: 1606 2870 3883 5294 5716 6431 8127 8804 9952 Size: 1264 1013 1411 422 715 1696 677 1148 Cuts at: 9952 10197 1606 Size: 245 2120 Fragments arranged by size: 2120 1696 1411 1264 1148 1013 715 677 422 245 NcoI C'CATG_G Cuts at: 513 1065 2004 5085 7837 513 Size: 552 939 3081 2752 3387 Fragments arranged by size: 3387 3081 2752 939 552 NdeI CA'TA_TG Cuts at: 387 7969 387 Size: 7582 3129 NheI G'CTAG_C Cuts at: 1052 3040 1052 Size: 1988 8723 NotI GC'GGCC_GC Cuts at: 8158 8158 Size: 10711 NsiI A_TGCA'T Cuts at: 6037 6037 Size: 10711 NspI r_CATG'y Cuts at: 1132 1229 3410 3838 4488 4600 4853 4976 5884 Size: 97 2181 428 650 112 253 123 908 Cuts at: 5884 6126 6169 7321 7372 7649 8069 9614 1132 Size: 242 43 1152 51 277 420 1545 2229 Fragments arranged by size: 2229 2181 1545 1152 908 650 428 420 277 253 242 123 112 97 51 43 PasI CC'CwG_GG Cuts at: 1348 6860 1348 Size: 5512 5199 PciI A'CATG_T Cuts at: 3406 9610 3406 Size: 6204 4507 PflMI CCAn_nnn'nTGG Cuts at: 1108 3560 7052 7204 7510 7607 7739 1108 Size: 2452 3492 152 306 97 132 4080 Fragments arranged by size: 4080 3492 2452 306 152 132 97 PfoI T'CCnGG_A Cuts at: 2679 2679 Size: 10711 PmeI GTTT'AAAC Cuts at: 8015 8015 Size: 10711 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2295 2327 8780 8812 10293 10325 2295 Size: 32 6453 32 1481 32 2681 Fragments arranged by size: 6453 2681 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 5438 5465 5438 Size: 27 10684 PshAI GACnn'nnGTC Cuts at: 1613 3526 1613 Size: 1913 8798 PsiI TTA'TAA Cuts at: 8281 8281 Size: 10711 PstI C_TGCA'G Cuts at: 830 2519 2875 3013 3484 5221 5677 6430 6820 Size: 1689 356 138 471 1737 456 753 390 Cuts at: 6820 8063 830 Size: 1243 3478 Fragments arranged by size: 3478 1737 1689 1243 753 471 456 390 356 138 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 10711 PvuII CAG'CTG Cuts at: 2870 3883 5294 6431 2870 Size: 1013 1411 1137 7150 Fragments arranged by size: 7150 1411 1137 1013 SacI G_AGCT'C Cuts at: 2137 2137 Size: 10711 SalI G'TCGA_C Cuts at: 1936 8053 1936 Size: 6117 4594 SbfI CC_TGCA'GG Cuts at: 8063 8063 Size: 10711 ScaI AGT'ACT Cuts at: 8959 10671 8959 Size: 1712 8999 SexAI A'CCwGG_T Cuts at: 3978 3978 Size: 10711 SfcI C'TryA_G Cuts at: 826 1045 2463 2515 2637 2871 3009 3480 3578 Size: 219 1418 52 122 234 138 471 98 Cuts at: 3578 4691 5217 5673 6426 6596 6816 7300 8059 Size: 1113 526 456 753 170 220 484 759 Cuts at: 8059 9194 9875 10066 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1418 1135 1113 759 753 681 526 484 471 456 234 220 219 191 170 138 122 98 52 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 10711 SgrAI Cr'CCGG_yG Cuts at: 1700 5787 1700 Size: 4087 6624 SmaI CCC'GGG Cuts at: 1872 8038 1872 Size: 6166 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3539 6810 8815 9716 9978 820 Size: 197 926 1596 3271 2005 901 262 1553 Fragments arranged by size: 3271 2005 1596 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 8502 493 Size: 8009 2702 SpeI A'CTAG_T Cuts at: 152 7498 152 Size: 7346 3365 SphI G_CATG'C Cuts at: 1132 4488 4853 7649 8069 1132 Size: 3356 365 2796 420 3774 Fragments arranged by size: 3774 3356 2796 420 365 SspI AAT'ATT Cuts at: 5 52 2702 4604 8635 5 Size: 47 2650 1902 4031 2081 Fragments arranged by size: 4031 2650 2081 1902 47 StuI AGG'CCT Cuts at: 1517 2888 3442 1517 Size: 1371 554 8786 Fragments arranged by size: 8786 1371 554 StyI C'CwwG_G Cuts at: 513 1065 2004 3851 3934 5085 5921 7046 7441 Size: 552 939 1847 83 1151 836 1125 395 Cuts at: 7441 7837 8150 513 Size: 396 313 3074 Fragments arranged by size: 3074 1847 1151 1125 939 836 552 396 395 313 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2817 3881 5587 7039 7243 8738 8755 Size: 1255 1237 1064 1706 1452 204 1495 17 Cuts at: 8755 8908 9093 9538 325 Size: 153 185 445 1498 Fragments arranged by size: 1706 1498 1495 1452 1255 1237 1064 445 204 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4429 5883 7147 Size: 274 80 33 51 1443 2452 1454 1264 Cuts at: 7147 8957 10669 96 Size: 1810 1712 138 Fragments arranged by size: 2452 1810 1712 1454 1443 1264 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 7578 7610 10671 10703 7578 Size: 32 3061 32 7586 Fragments arranged by size: 7586 3061 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2046 6667 7930 1316 Size: 730 4621 1263 4097 Fragments arranged by size: 4621 4097 1263 730 XbaI T'CTAG_A Cuts at: 7217 8047 7217 Size: 830 9881 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2357 4721 7049 7186 7385 2357 Size: 2364 2328 137 199 5683 Fragments arranged by size: 5683 2364 2328 199 137 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 10711 XmaI C'CCGG_G Cuts at: 1870 8036 1870 Size: 6166 4545 XmnI GAAnn'nnTTC Cuts at: 4356 8840 4356 Size: 4484 6227 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 8519 276 Size: 53 83 186 936 6985 2468 Fragments arranged by size: 6985 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BsiWI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI