################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AvaI AvrII BamHI BbeI BbrPI BbsI BcgI BcnI BfrI BlnI BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstXI BstZ17I Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FauI FseI HaeII HapII Hin1I HpaI HpaII KasI KpnI KspI MamI MluI MroI MspI MvnI NaeI NarI NciI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AflIII 1401 ACATGT A^C[GA][CT]GT Alw44I 1978 GTGCAC G^TGCAC ApaLI 1978 GTGCAC G^TGCAC Asp700I 2347 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 38 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaII 1941 GGACC G^G[AT]CC AviII 2733 TGCGCA TGC^GCA BalI 2328 TGGCCA TGG^CCA BanI 1368 GGCACC G^G[CT][GA]CC BbvCI 1246 CCTCAGC CCTCAGC(-5/-2) BciVI 3360 GGATAC GTATCC(6/5) BclI 2301 TGATCA T^GATCA BglI 1833 GCCTGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 2743 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2743 GCTCAGC GC^T[ACGT]AGC BsaI 3076 GAGACC GGTCTC(1/5) BspLU11I 1401 ACATGT A^CATGT BspMI 3036 ACCTGC ACCTGC(4/8) BssSI 1646 CACGAG CACGAG(-5/-1) BstAPI 2586 GCATCACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 2743 GCTCAGC GC^T[ACGT]AGC CfoI 2734 GCGC GCG^C DraI 1110 TTTAAA TTT^AAA DraII 1703 AGGCCCC [GA]G^G[ACGT]CC[CT] EaeI 2328 TGGCCA [CT]^GGCC[GA] EcoNI 1255 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 1703 AGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 1051 GAATTC G^AATTC FbaI 2301 TGATCA T^GATCA FspI 2733 TGCGCA TGC^GCA HgaI 1039 GCGTC GACGC(5/10) HhaI 2734 GCGC GCG^C HinP1I 2734 GCGC G^CGC MfeI 1207 CAATTG C^AATTG MluNI 2328 TGGCCA TGG^CCA MscI 2328 TGGCCA TGG^CCA MunI 1207 CAATTG C^AATTG NcoI 3080 CCATGG C^CATGG NheI 1035 GCTAGC G^CTAGC PflFI 38 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1549 CCAATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 1517 GACCAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 128 GAGCTC GAGCT^C SexAI 1973 ACCAGGT A^CC[AT]GGT SmlI 1534 CTCAAG C^T[CT][GA]AG Tth111I 38 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1549 CCAATTTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmnI 2347 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AlwI 2616 GATCC GGATC(4/5) 2317 GGATC AocI 809 CCTCAGG CC^T[ACGT]AGG 1363 CCTGAGG BglII 1650 AGATCT A^GATCT 2628 AGATCT BseRI 1226 CTCCTC GAGGAG(10/8) 1229 CTCCTC BsrDI 717 CATTGC GCAATG(2/0) 925 GCAATG BstYI 1650 AGATCT [GA]^GATC[CT] 2628 AGATCT Bsu36I 809 CCTCAGG CC^T[ACGT]AGG 1363 CCTGAGG DsaI 650 CCGTGG C^C[GA][CT]GG 3080 CCATGG EarI 645 CTCTTC CTCTTC(1/4) 1232 CTCTTC Eco81I 809 CCTCAGG CC^T[ACGT]AGG 1363 CCTGAGG HincII 410 GTCAAC GT[CT]^[GA]AC 2723 GTCAAC HindII 410 GTCAAC GT[CT]^[GA]AC 2723 GTCAAC HindIII 1853 AAGCTT A^AGCTT 2675 AAGCTT Ksp632I 645 CTCTTC CTCTTC(1/4) 1232 CTCTTC MaeII 366 ACGT A^CGT 1740 ACGT MflI 1650 AGATCT [GA]^GATC[CT] 2628 AGATCT SphI 2479 GCATGC GCATG^C 2844 GCATGC SspI 695 AATATT AAT^ATT 2597 AATATT StuI 881 AGGCCT AGG^CCT 1435 AGGCCT TaqI 1629 TCGA T^CGA 2390 TCGA TthHB8I 1629 TCGA T^CGA 2390 TCGA XcmI 345 CCAAAAGGCTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2709 CCAAGATATGTCTGG XhoII 1650 AGATCT [GA]^GATC[CT] 2628 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcsI AfaI AluI AlwNI ApoI AspHI BanII BbvI BfaI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraIII EcoRII EcoT14I Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI