################################## # NO CUT ENZYMES ( 146 enzymes) # ################################## AatII AccI AccII AccIII AciI AclI AcyI AflII AgeI AlwNI ApaI AscI Asp700I AspI AvaI AvaII AviII AvrII BamHI BbeI BbrPI BcgI BcnI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstZ17I Cac8I Cfr10I Cfr13I ClaI CpoI DraII DrdI EagI EarI EclXI Eco52I EcoNI EcoO109I EcoO65I EcoRV EcoT14I EcoT22I FauI FseI FspI HapII HgaI Hin1I HincII HindII HpaI HpaII KasI Ksp632I KspI MamI MfeI MluI MroI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RcaI RsrII SacII SalI SapI Sau96I SbfI ScaI SfaNI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tth111I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 555 GGTACC G^GTACC AflIII 662 ACATGT A^C[GA][CT]GT AhdI 604 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 170 GTGCAC G^TGCAC AocI 859 CCTAAGG CC^T[ACGT]AGG Aor51HI 1273 AGCGCT AGC^GCT ApaLI 170 GTGCAC G^TGCAC AseI 1291 ATTAAT AT^TAAT AsnI 1291 ATTAAT AT^TAAT Asp718I 555 GGTACC G^GTACC AspEI 604 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BbvCI 968 CCTCAGC CCTCAGC(-5/-2) BciVI 1061 GGATAC GTATCC(6/5) BfaI 157 CTAG C^TAG BglII 371 AGATCT A^GATCT BlpI 1269 GCTTAGC GC^T[ACGT]AGC BpmI 521 CTCCAG CTGGAG(16/14) Bpu1102I 1269 GCTTAGC GC^T[ACGT]AGC BsmFI 1156 GTCCC GGGAC(10/14) BsmI 1314 GAATGC GAATGC(1/-1) BspLU11I 662 ACATGT A^CATGT BspMI 843 ACCTGC ACCTGC(4/8) BsrDI 1200 CATTGC GCAATG(2/0) Bsu36I 859 CCTAAGG CC^T[ACGT]AGG CelII 1269 GCTTAGC GC^T[ACGT]AGC DraI 480 TTTAAA TTT^AAA DraIII 355 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 1149 CCACGG C^C[GA][CT]GG Eam1105I 604 GACCAAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1273 AGCGCT AGC^GCT Eco81I 859 CCTAAGG CC^T[ACGT]AGG HaeII 1273 AGCGCT [GA]GCGC^[CT] KpnI 555 GGTACC GGTAC^C MaeI 157 CTAG C^TAG MaeII 727 ACGT A^CGT NspI 662 ACATGT [GA]CATG^[CT] PflMI 1458 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1154 GAGTC GAGTC(4/5) PshBI 1291 ATTAAT AT^TAAT SexAI 1444 ACCTGGT A^CC[AT]GGT SmlI 510 CTTGAG C^T[CT][GA]AG Van91I 1458 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### BalI 76 TGGCCA TGG^CCA 332 TGGCCA BanI 225 GGTGCC G^G[CT][GA]CC 555 GGTACC BanII 124 GAGCTC G[GA]GC[CT]^C 885 GAGCTC BbsI 716 GAAGAC GAAGAC(2/6) 1375 GTCTTC BbvI 1225 GCAGC GCAGC(8/12) 1429 GCAGC BclI 1185 TGATCA T^GATCA 1344 TGATCA BpuAI 716 GAAGAC GAAGAC(2/6) 1375 GTCTTC BsaJI 1149 CCACGG C^C[ACGT][ACGT]GG 1361 CCCTGG BsrI 451 CCAGT ACTGG(1/-1) 1452 CCAGT BstF5I 1087 CATCC GGATG(2/0) 1467 GGATG BstYI 371 AGATCT [GA]^GATC[CT] 1130 GGATCT CfoI 1274 GCGC GCG^C 1398 GCGC EaeI 76 TGGCCA [CT]^GGCC[GA] 332 TGGCCA EcoRI 491 GAATTC G^AATTC 1334 GAATTC FbaI 1185 TGATCA T^GATCA 1344 TGATCA Fnu4HI 1225 GCAGC GC^[ACGT]GC 1429 GCAGC FokI 1087 CATCC GGATG(9/13) 1467 GGATG HhaI 1274 GCGC GCG^C 1398 GCGC HinP1I 1274 GCGC G^CGC 1398 GCGC HindIII 707 AAGCTT A^AGCTT 1100 AAGCTT ItaI 1225 GCAGC GC^[ACGT]GC 1429 GCAGC MaeIII 148 GTGAC ^GT[ACGT]AC 1495 GTGAC MflI 371 AGATCT [GA]^GATC[CT] 1130 GGATCT MluNI 76 TGGCCA TGG^CCA 332 TGGCCA MscI 76 TGGCCA TGG^CCA 332 TGGCCA MspA1I 400 CAGCTG C[AC]G^C[GT]G 1226 CAGCTG MwoI 163 GCCTCTGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1216 GCCAAAGGAGC PvuII 400 CAGCTG CAG^CTG 1226 CAGCTG SacI 124 GAGCTC GAGCT^C 885 GAGCTC SfcI 652 CTACAG C^T[GA][CT]AG 699 CTATAG TaqI 1368 TCGA T^CGA 1385 TCGA TseI 1225 GCAGC G^C[AT]GC 1429 GCAGC Tsp45I 148 GTGAC ^GT[GC]AC 1495 GTGAC TthHB8I 1368 TCGA T^CGA 1385 TCGA XhoII 371 AGATCT [GA]^GATC[CT] 1130 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 35 enzymes) # ######################################### AcsI AfaI AluI AlwI ApoI AspHI BmyI BsiHKAI BsiYI BslI BsmAI Bsp1286I BssKI BstNI DdeI DpnII EcoRII HaeIII HinfI HphI MboI MboII MnlI MseI MvaI NdeII NlaIII NlaIV RsaI Sau3AI ScrFI TfiI Tru9I Tsp509I TspRI