(Circular) (Six-base) MAPSORT of: pFN21AE2050.seq Check: 8713 from: 1 to: 6718 pFN21AE2050 6718 bp With 182 enzymes: SgfI * August 16, 2010 14:47 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4528 278 Size: 53 83 186 936 2992 2468 Fragments arranged by size: 2992 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4061 1937 Size: 2124 4594 Acc65I G'GTAC_C Cuts at: 4039 4039 Size: 6718 AclI AA'CG_TT Cuts at: 1563 3751 4845 5218 1563 Size: 2188 1094 373 3063 Fragments arranged by size: 3063 2188 1094 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2357 2631 2702 3071 4781 6165 6433 1632 Size: 725 274 71 369 1710 1384 268 1917 Fragments arranged by size: 1917 1710 1384 725 369 274 268 71 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6521 AflIII A'CryG_T Cuts at: 1203 5617 6594 1203 Size: 4414 977 1327 Fragments arranged by size: 4414 1327 977 AgeI A'CCGG_T Cuts at: 2061 5549 2061 Size: 3488 3230 AhdI GACnn_n'nnGTC Cuts at: 5447 5447 Size: 6718 AleI CACnn'nnGTG Cuts at: 1166 2222 1166 Size: 1056 5662 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6718 AlwNI CAG_nnn'CTG Cuts at: 2039 3908 4461 6033 2039 Size: 1869 553 1572 2724 Fragments arranged by size: 2724 1869 1572 553 ApaLI G'TGCA_C Cuts at: 2556 4775 5931 2556 Size: 2219 1156 3343 Fragments arranged by size: 3343 2219 1156 ApoI r'AATT_y Cuts at: 1493 2924 3092 3153 3571 4027 4253 4405 6514 Size: 1431 168 61 418 456 226 152 2109 Cuts at: 6514 1493 Size: 1697 Fragments arranged by size: 2109 1697 1431 456 418 226 168 152 61 AseI AT'TA_AT Cuts at: 160 1032 5272 160 Size: 872 4240 1606 Fragments arranged by size: 4240 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6718 AvaI C'yCGr_G Cuts at: 1870 1943 3008 4043 1870 Size: 73 1065 1035 4545 Fragments arranged by size: 4545 1065 1035 73 BamHI G'GATC_C Cuts at: 4048 4415 4048 Size: 367 6351 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4039 5494 618 Size: 325 217 627 2252 1455 1842 Fragments arranged by size: 2252 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3961 4037 1391 Size: 2570 76 4072 Fragments arranged by size: 4072 2570 76 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2306 2646 2667 3280 928 Size: 971 407 340 21 613 4366 Fragments arranged by size: 4366 971 613 407 340 21 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4909 4943 1903 Size: 34 2972 34 3678 Fragments arranged by size: 3678 2972 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4610 5820 4610 Size: 1210 5508 BclI T'GATC_A Cuts at: 1548 1548 Size: 6718 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2076 5329 136 Size: 107 122 71 631 605 404 3253 1525 Fragments arranged by size: 3253 1525 631 605 404 122 107 71 BglII A'GATC_T Cuts at: 2404 2614 6713 2404 Size: 210 4099 2409 Fragments arranged by size: 4099 2409 210 BlpI GC'TnA_GC Cuts at: 2389 4135 2389 Size: 1746 4972 Bme1580I G_kGCm'C Cuts at: 1790 2560 4779 5935 1790 Size: 770 2219 1156 2573 Fragments arranged by size: 2573 2219 1156 770 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6718 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4449 5407 442 Size: 416 53 83 399 24 3032 958 1753 Fragments arranged by size: 3032 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6718 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3639 3945 5378 1239 Size: 2400 306 1433 2579 Fragments arranged by size: 2579 2400 1433 306 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2081 3144 4843 5708 6006 2081 Size: 1063 1699 865 298 2793 Fragments arranged by size: 2793 1699 1063 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5381 882 Size: 617 3882 2219 Fragments arranged by size: 3882 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4509 493 Size: 711 3305 2702 Fragments arranged by size: 3305 2702 711 BsaBI GATnn'nnATC Cuts at: 3105 6712 3105 Size: 3607 3111 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4525 4907 5542 275 Size: 53 83 186 936 2992 382 635 1451 Fragments arranged by size: 2992 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2061 5151 5549 5823 5970 6381 2061 Size: 3090 398 274 147 411 2398 Fragments arranged by size: 3090 2398 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2228 2258 1178 Size: 30 165 30 825 30 5638 Fragments arranged by size: 5638 825 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2453 2747 3218 1186 Size: 1267 294 471 4686 Fragments arranged by size: 4686 1267 471 294 BseYI C'CCAG_C Cuts at: 3042 4014 5921 6417 3042 Size: 972 1907 496 3343 Fragments arranged by size: 3343 1907 972 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6145 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4168 4929 5957 1540 Size: 275 186 2167 761 1028 2301 Fragments arranged by size: 2301 2167 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2560 4779 4864 5935 2560 Size: 2219 85 1071 3343 Fragments arranged by size: 3343 2219 1071 85 BsmI GAATG_Cn' Cuts at: 2296 2390 4229 4322 2296 Size: 94 1839 93 4692 Fragments arranged by size: 4692 1839 94 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2560 3961 4037 4779 4864 5935 1391 Size: 399 770 1401 76 742 85 1071 2174 Fragments arranged by size: 2174 1401 1071 770 742 399 85 76 BspEI T'CCGG_A Cuts at: 6381 6381 Size: 6718 BspHI T'CATG_A Cuts at: 4607 6337 4607 Size: 1730 4988 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2047 3958 4073 844 Size: 1203 1911 115 3489 Fragments arranged by size: 3489 1911 1203 115 BsrBI CCG'CTC Cuts at: 1394 1823 2013 4605 1394 Size: 429 190 2592 3507 Fragments arranged by size: 3507 2592 429 190 BsrDI GCAATG_nn' Cuts at: 65 1248 5213 5387 65 Size: 1183 3965 174 1396 Fragments arranged by size: 3965 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2061 5362 5549 1700 Size: 110 251 3301 187 2869 Fragments arranged by size: 3301 2869 251 187 110 BsrGI T'GTAC_A Cuts at: 96 2949 96 Size: 2853 3865 BssHII G'CGCG_C Cuts at: 1925 2089 1925 Size: 164 6554 BssSI C'ACGA_G Cuts at: 4778 5790 4778 Size: 1012 5706 BstAPI GCAn_nnn'nTGC Cuts at: 794 2468 794 Size: 1674 5044 BstBI TT'CG_AA Cuts at: 5522 5522 Size: 6718 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6718 BstXI CCAn_nnnn'nTGG Cuts at: 3732 3732 Size: 6718 BstYI r'GATC_y Cuts at: 1972 2404 2614 3487 3721 4048 4415 4800 4817 Size: 432 210 873 234 327 367 385 17 Cuts at: 4817 6269 6355 6367 6713 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 873 432 385 367 346 327 234 210 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 3138 513 Size: 552 939 1134 4093 Fragments arranged by size: 4093 1134 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5979 Fragments arranged by size: 5979 683 56 BtsI GCAGTG_nn' Cuts at: 2400 2547 3898 4234 5028 5048 2400 Size: 147 1351 336 794 20 4070 Fragments arranged by size: 4070 1351 794 336 147 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6718 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2763 2798 3404 3439 529 Size: 35 2199 35 606 35 3808 Fragments arranged by size: 3808 2199 606 35 35 35 DraI TTT'AAA Cuts at: 4022 4022 Size: 6718 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2837 5725 809 Size: 507 1521 2888 1802 Fragments arranged by size: 2888 1802 1521 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2068 2679 4165 Size: 54 1425 50 275 18 238 611 1486 Cuts at: 4165 5054 6683 8 Size: 889 1629 43 Fragments arranged by size: 1629 1486 1425 889 611 275 238 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4165 1537 Size: 275 2353 4090 Fragments arranged by size: 4090 2353 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4648 1353 Size: 3295 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3403 5305 5679 5825 387 Size: 1064 138 1814 1902 374 146 1280 Fragments arranged by size: 1902 1814 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2357 2631 2702 3071 3639 3945 4781 Size: 393 725 274 71 369 568 306 836 Cuts at: 4781 5378 6165 6433 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 836 787 725 597 568 393 369 306 274 268 71 EcoO109I rG'GnC_Cy Cuts at: 3807 3807 Size: 6718 EcoRI G'AATT_C Cuts at: 4027 4405 4027 Size: 378 6340 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2957 2957 Size: 6718 FspI TGC'GCA Cuts at: 5224 5224 Size: 6718 HaeII r_GCGC'y Cuts at: 1678 5865 1678 Size: 4187 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2394 2426 3524 3556 5359 Size: 32 135 32 822 32 1098 32 1803 Cuts at: 5359 5391 5433 5465 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1803 1098 822 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3629 4062 4308 669 Size: 988 281 1691 433 246 3079 Fragments arranged by size: 3079 1691 988 433 281 246 HindIII A'AGCT_T Cuts at: 748 2193 2894 2934 748 Size: 1445 701 40 4532 Fragments arranged by size: 4532 1445 701 40 HpaI GTT'AAC Cuts at: 4308 4308 Size: 6718 KpnI G_GTAC'C Cuts at: 4043 4043 Size: 6718 MfeI C'AATT_G Cuts at: 2482 4317 2482 Size: 1835 4883 MluI A'CGCG_T Cuts at: 6594 6594 Size: 6718 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5832 6016 1305 Size: 4527 184 2007 Fragments arranged by size: 4527 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2070 2681 10 Size: 54 1425 343 238 611 4047 Fragments arranged by size: 4047 1425 611 343 238 54 MslI CAynn'nnrTG Cuts at: 518 1166 2222 4676 5035 5194 518 Size: 648 1056 2454 359 159 2042 Fragments arranged by size: 2454 2042 1056 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2022 2578 3122 3421 4134 4811 5959 6204 Size: 416 556 544 299 713 677 1148 245 Cuts at: 6204 1606 Size: 2120 Fragments arranged by size: 2120 1148 713 677 556 544 416 299 245 NcoI C'CATG_G Cuts at: 513 1065 2004 3138 513 Size: 552 939 1134 4093 Fragments arranged by size: 4093 1134 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6718 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6718 NotI GC'GGCC_GC Cuts at: 4165 4165 Size: 6718 NspI r_CATG'y Cuts at: 1132 1229 2556 3183 4076 5621 1132 Size: 97 1327 627 893 1545 2229 Fragments arranged by size: 2229 1545 1327 893 627 97 PasI CC'CwG_GG Cuts at: 1348 3811 1348 Size: 2463 4255 PciI A'CATG_T Cuts at: 5617 5617 Size: 6718 PflMI CCAn_nnn'nTGG Cuts at: 1108 3887 1108 Size: 2779 3939 PmeI GTTT'AAAC Cuts at: 4022 4022 Size: 6718 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4787 4819 6300 6332 4787 Size: 32 1481 32 5173 Fragments arranged by size: 5173 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3807 3807 Size: 6718 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6718 PsiI TTA'TAA Cuts at: 4288 4288 Size: 6718 PstI C_TGCA'G Cuts at: 830 2715 3826 3955 4070 830 Size: 1885 1111 129 115 3478 Fragments arranged by size: 3478 1885 1111 129 115 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6718 PvuII CAG'CTG Cuts at: 2578 3122 3421 2578 Size: 544 299 5875 Fragments arranged by size: 5875 544 299 SalI G'TCGA_C Cuts at: 1936 4060 1936 Size: 2124 4594 SanDI GG'GwC_CC Cuts at: 3807 3807 Size: 6718 SbfI CC_TGCA'GG Cuts at: 3955 4070 3955 Size: 115 6603 ScaI AGT'ACT Cuts at: 4966 6678 4966 Size: 1712 5006 SfcI C'TryA_G Cuts at: 826 1045 2117 2711 2919 3822 3951 4066 5201 Size: 219 1072 594 208 903 129 115 1135 Cuts at: 5201 5882 6073 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1072 903 681 594 219 208 191 129 115 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6718 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6718 SmaI CCC'GGG Cuts at: 1872 4045 1872 Size: 2173 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2096 3159 4822 5723 5985 820 Size: 197 926 153 1063 1663 901 262 1553 Fragments arranged by size: 1663 1553 1063 926 901 262 197 153 SnaBI TAC'GTA Cuts at: 493 4509 493 Size: 4016 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6718 SphI G_CATG'C Cuts at: 1132 4076 1132 Size: 2944 3774 SspI AAT'ATT Cuts at: 5 52 3169 3189 4642 5 Size: 47 3117 20 1453 2081 Fragments arranged by size: 3117 2081 1453 47 20 StuI AGG'CCT Cuts at: 1517 1517 Size: 6718 StyI C'CwwG_G Cuts at: 513 1065 2004 2569 3138 4157 513 Size: 552 939 565 569 1019 3074 Fragments arranged by size: 3074 1019 939 569 565 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3886 4745 4762 4915 5100 5545 325 Size: 1255 2306 859 17 153 185 445 1498 Fragments arranged by size: 2306 1498 1255 859 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2268 2949 4964 Size: 274 80 33 51 1443 291 681 2015 Cuts at: 4964 6676 96 Size: 1712 138 Fragments arranged by size: 2015 1712 1443 681 291 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3285 3317 3589 3621 6678 6710 3285 Size: 32 272 32 3057 32 3293 Fragments arranged by size: 3293 3057 272 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6718 XbaI T'CTAG_A Cuts at: 4054 4054 Size: 6718 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6718 XmaI C'CCGG_G Cuts at: 1870 4043 1870 Size: 2173 4545 XmnI GAAnn'nnTTC Cuts at: 4847 4847 Size: 6718 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4526 276 Size: 53 83 186 936 2992 2468 Fragments arranged by size: 2992 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI