(Linear) (Six-base) MAPSORT of: KIEE2050.seq Check: 4699 from: 1 to: 2013 KIEE2050.orf With 182 enzymes: SgfI * July 15, 2010 18:53 .. AclI AA'CG_TT Cuts at: 0 1746 2013 Size: 1746 267 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 352 626 697 1066 2013 Size: 352 274 71 369 947 Fragments arranged by size: 947 369 352 274 71 AgeI A'CCGG_T Cuts at: 0 56 2013 Size: 56 1957 AleI CACnn'nnGTG Cuts at: 0 217 2013 Size: 217 1796 AlwNI CAG_nnn'CTG Cuts at: 0 34 1903 2013 Size: 34 1869 110 Fragments arranged by size: 1869 110 34 ApaLI G'TGCA_C Cuts at: 0 551 2013 Size: 551 1462 ApoI r'AATT_y Cuts at: 0 919 1087 1148 1566 2013 Size: 919 168 61 418 447 Fragments arranged by size: 919 447 418 168 61 AvaI C'yCGr_G Cuts at: 0 1003 2013 Size: 1003 1010 BanII G_rGCy'C Cuts at: 0 1956 2013 Size: 1956 57 BbsI GAAGACnn'nnnn_ Cuts at: 0 301 641 662 1275 2013 Size: 301 340 21 613 738 Fragments arranged by size: 738 613 340 301 21 BglI GCCn_nnn'nGGC Cuts at: 0 71 2013 Size: 71 1942 BglII A'GATC_T Cuts at: 0 399 609 2013 Size: 399 210 1404 Fragments arranged by size: 1404 399 210 BlpI GC'TnA_GC Cuts at: 0 384 2013 Size: 384 1629 Bme1580I G_kGCm'C Cuts at: 0 555 2013 Size: 555 1458 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1634 1940 2013 Size: 1634 306 73 Fragments arranged by size: 1634 306 73 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 76 1139 2013 Size: 76 1063 874 Fragments arranged by size: 1063 874 76 BsaBI GATnn'nnATC Cuts at: 0 1100 2013 Size: 1100 913 BsaWI w'CCGG_w Cuts at: 0 56 2013 Size: 56 1957 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 223 253 2013 Size: 223 30 1760 Fragments arranged by size: 1760 223 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 448 742 1213 2013 Size: 448 294 471 800 Fragments arranged by size: 800 471 448 294 BseYI C'CCAG_C Cuts at: 0 1037 2013 Size: 1037 976 BsiHKAI G_wGCw'C Cuts at: 0 555 2013 Size: 555 1458 BsmI GAATG_Cn' Cuts at: 0 291 385 2013 Size: 291 94 1628 Fragments arranged by size: 1628 291 94 Bsp1286I G_dGCh'C Cuts at: 0 555 1956 2013 Size: 555 1401 57 Fragments arranged by size: 1401 555 57 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 42 1953 2013 Size: 42 1911 60 Fragments arranged by size: 1911 60 42 BsrBI CCG'CTC Cuts at: 0 8 2013 Size: 8 2005 BsrFI r'CCGG_y Cuts at: 0 56 2013 Size: 56 1957 BsrGI T'GTAC_A Cuts at: 0 944 2013 Size: 944 1069 BssHII G'CGCG_C Cuts at: 0 84 2013 Size: 84 1929 BstAPI GCAn_nnn'nTGC Cuts at: 0 463 2013 Size: 463 1550 BstXI CCAn_nnnn'nTGG Cuts at: 0 1727 2013 Size: 1727 286 BstYI r'GATC_y Cuts at: 0 399 609 1482 1716 2013 Size: 399 210 873 234 297 Fragments arranged by size: 873 399 297 234 210 BtgI C'CryG_G Cuts at: 0 1133 2013 Size: 1133 880 BtsI GCAGTG_nn' Cuts at: 0 395 542 1893 2013 Size: 395 147 1351 120 Fragments arranged by size: 1351 395 147 120 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 758 793 1399 1434 2013 Size: 758 35 606 35 579 Fragments arranged by size: 758 606 579 35 35 DrdI GACnn_nn'nnGTC Cuts at: 0 832 2013 Size: 832 1181 EaeI y'GGCC_r Cuts at: 0 63 674 2013 Size: 63 611 1339 Fragments arranged by size: 1339 611 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1398 2013 Size: 1398 615 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 352 626 697 1066 1634 1940 2013 Size: 352 274 71 369 568 306 73 Fragments arranged by size: 568 369 352 306 274 73 71 EcoO109I rG'GnC_Cy Cuts at: 0 1802 2013 Size: 1802 211 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 952 2013 Size: 952 1061 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 389 421 1519 1551 2013 Size: 389 32 1098 32 462 Fragments arranged by size: 1098 462 389 32 32 HincII GTy'rAC Cuts at: 0 1624 2013 Size: 1624 389 HindIII A'AGCT_T Cuts at: 0 188 889 929 2013 Size: 188 701 40 1084 Fragments arranged by size: 1084 701 188 40 MfeI C'AATT_G Cuts at: 0 477 2013 Size: 477 1536 MscI TGG'CCA Cuts at: 0 65 676 2013 Size: 65 611 1337 Fragments arranged by size: 1337 611 65 MslI CAynn'nnrTG Cuts at: 0 217 2013 Size: 217 1796 MspA1I CmG'CkG Cuts at: 0 17 573 1117 1416 2013 Size: 17 556 544 299 597 Fragments arranged by size: 597 556 544 299 17 NcoI C'CATG_G Cuts at: 0 1133 2013 Size: 1133 880 NspI r_CATG'y Cuts at: 0 551 1178 2013 Size: 551 627 835 Fragments arranged by size: 835 627 551 PasI CC'CwG_GG Cuts at: 0 1806 2013 Size: 1806 207 PflMI CCAn_nnn'nTGG Cuts at: 0 1882 2013 Size: 1882 131 PpuMI rG'GwC_Cy Cuts at: 0 1802 2013 Size: 1802 211 PstI C_TGCA'G Cuts at: 0 710 1821 1950 2013 Size: 710 1111 129 63 Fragments arranged by size: 1111 710 129 63 PvuII CAG'CTG Cuts at: 0 573 1117 1416 2013 Size: 573 544 299 597 Fragments arranged by size: 597 573 544 299 SanDI GG'GwC_CC Cuts at: 0 1802 2013 Size: 1802 211 SbfI CC_TGCA'GG Cuts at: 0 1950 2013 Size: 1950 63 SfcI C'TryA_G Cuts at: 0 112 706 914 1817 1946 2013 Size: 112 594 208 903 129 67 Fragments arranged by size: 903 594 208 129 112 67 SmlI C'TyrA_G Cuts at: 0 91 1154 2013 Size: 91 1063 859 Fragments arranged by size: 1063 859 91 SspI AAT'ATT Cuts at: 0 1164 1184 2013 Size: 1164 20 829 Fragments arranged by size: 1164 829 20 StyI C'CwwG_G Cuts at: 0 564 1133 2013 Size: 564 569 880 Fragments arranged by size: 880 569 564 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1881 2013 Size: 1881 132 TatI w'GTAC_w Cuts at: 0 263 944 2013 Size: 263 681 1069 Fragments arranged by size: 1069 681 263 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1280 1312 1584 1616 2013 Size: 1280 32 272 32 397 Fragments arranged by size: 1280 397 272 32 32 Enzymes that do cut: AclI AcuI AgeI AleI AlwNI ApaLI ApoI AvaI BanII BbsI BglI BglII BlpI Bme1580I BpmI BpuEI BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrFI BsrGI BssHII BstAPI BstXI BstYI BtgI BtsI CspCI DrdI EaeI EciI Eco57MI EcoO109I FalI Hin4I HincII HindIII MfeI MscI MslI MspA1I NcoI NspI PasI PflMI PpuMI PstI PvuII SanDI SbfI SfcI SmlI SspI StyI TaqII TatI TstI Enzymes that do not cut: AarI AatII AccI Acc65I AfeI AflII AflIII AhdI AlfI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BmgBI BmrI BmtI BplI Bpu10I BsaI BsaAI BsaHI BsgI BsiEI BsiWI BsmBI BspEI BspHI BsrDI BssSI BstBI BstEII BstZ17I Bsu36I BtgZI ClaI DraI DraIII EagI EarI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII HpaI KasI KpnI MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI