(Circular) (Six-base) MAPSORT of: pFN21AB5621.seq Check: 2649 from: 1 to: 8773 pFN21AB5621 8773 bp With 182 enzymes: SgfI * March 28, 2012 12:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4220 4220 Size: 8773 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5268 6583 278 Size: 53 83 186 936 3732 1315 2468 Fragments arranged by size: 3732 2468 1315 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 6116 1937 Size: 4179 4594 Acc65I G'GTAC_C Cuts at: 6094 6094 Size: 8773 AclI AA'CG_TT Cuts at: 1563 6900 7273 1563 Size: 5337 373 3063 Fragments arranged by size: 5337 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4415 4526 5001 5913 6836 8220 8488 1632 Size: 2783 111 475 912 923 1384 268 1917 Fragments arranged by size: 2783 1917 1384 923 912 475 268 111 AflII C'TTAA_G Cuts at: 820 1017 3890 820 Size: 197 2873 5703 Fragments arranged by size: 5703 2873 197 AflIII A'CryG_T Cuts at: 1203 2478 3335 7672 8649 1203 Size: 1275 857 4337 977 1327 Fragments arranged by size: 4337 1327 1275 977 857 AgeI A'CCGG_T Cuts at: 6011 7604 6011 Size: 1593 7180 AhdI GACnn_n'nnGTC Cuts at: 3651 5269 7502 3651 Size: 1618 2233 4922 Fragments arranged by size: 4922 2233 1618 AleI CACnn'nnGTG Cuts at: 1166 4319 5954 1166 Size: 3153 1635 3985 Fragments arranged by size: 3985 3153 1635 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2036 5520 1184 Size: 852 3484 4437 Fragments arranged by size: 4437 3484 852 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4650 4682 4650 Size: 32 8741 AlwNI CAG_nnn'CTG Cuts at: 3775 5674 6516 8088 3775 Size: 1899 842 1572 4460 Fragments arranged by size: 4460 1899 1572 842 ApaI G_GGCC'C Cuts at: 5386 5386 Size: 8773 ApaLI G'TGCA_C Cuts at: 3715 6830 7986 3715 Size: 3115 1156 4502 Fragments arranged by size: 4502 3115 1156 ApoI r'AATT_y Cuts at: 1493 2532 2864 4487 4754 4763 4805 5345 5636 Size: 1039 332 1623 267 9 42 540 291 Cuts at: 5636 6082 6308 6460 8569 1493 Size: 446 226 152 2109 1697 Fragments arranged by size: 2109 1697 1623 1039 540 446 332 291 267 226 152 42 9 AseI AT'TA_AT Cuts at: 160 1032 2616 7327 160 Size: 872 1584 4711 1606 Fragments arranged by size: 4711 1606 1584 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8773 AvaI C'yCGr_G Cuts at: 1870 1943 2055 2456 5154 6043 6098 1870 Size: 73 112 401 2698 889 55 4545 Fragments arranged by size: 4545 2698 889 401 112 73 55 BamHI G'GATC_C Cuts at: 4423 6103 6470 4423 Size: 1680 367 6726 Fragments arranged by size: 6726 1680 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3317 4000 4271 4532 4712 Size: 325 217 627 1530 683 271 261 180 Cuts at: 4712 5860 6094 7549 618 Size: 1148 234 1455 1842 Fragments arranged by size: 1842 1530 1455 1148 683 627 325 271 261 234 217 180 BanII G_rGCy'C Cuts at: 1391 3414 4648 4919 5386 5445 6062 6092 1391 Size: 2023 1234 271 467 59 617 30 4072 Fragments arranged by size: 4072 2023 1234 617 467 271 59 30 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2590 3953 4137 4403 5829 928 Size: 971 691 1363 184 266 1426 3872 Fragments arranged by size: 3872 1426 1363 971 691 266 184 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 6964 6998 1903 Size: 34 50 34 4943 34 3678 Fragments arranged by size: 4943 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3817 5087 6665 7875 3817 Size: 1270 1578 1210 4715 Fragments arranged by size: 4715 1578 1270 1210 BclI T'GATC_A Cuts at: 1548 2577 3879 1548 Size: 1029 1302 6442 Fragments arranged by size: 6442 1302 1029 BfrBI ATG'CAT Cuts at: 3899 3899 Size: 8773 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4732 7384 136 Size: 107 122 71 631 605 3060 2652 1525 Fragments arranged by size: 3060 2652 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2369 3728 4984 8768 2369 Size: 1359 1256 3784 2374 Fragments arranged by size: 3784 2374 1359 1256 BlpI GC'TnA_GC Cuts at: 2105 4918 5508 6190 2105 Size: 2813 590 682 4688 Fragments arranged by size: 4688 2813 682 590 Bme1580I G_kGCm'C Cuts at: 1790 3719 4276 4834 5386 5865 6834 7990 1790 Size: 1929 557 558 552 479 969 1156 2573 Fragments arranged by size: 2573 1929 1156 969 558 557 552 479 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 8773 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2791 2836 5439 Size: 416 53 83 399 24 1374 45 2603 Cuts at: 5439 6504 7462 442 Size: 1065 958 1753 Fragments arranged by size: 2603 1753 1374 1065 958 416 399 83 53 45 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8773 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2712 4754 6081 2712 Size: 2042 1327 5404 Fragments arranged by size: 5404 2042 1327 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2319 4043 5947 6017 7433 1239 Size: 1080 1724 1904 70 1416 2579 Fragments arranged by size: 2579 1904 1724 1416 1080 70 Bpu10I CC'TnA_GC Cuts at: 5043 5043 Size: 8773 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3137 3921 4646 6898 7763 8061 3137 Size: 784 725 2252 865 298 3849 Fragments arranged by size: 3849 2252 865 784 725 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2045 2823 3247 4112 4491 5446 7436 Size: 617 546 778 424 865 379 955 1990 Cuts at: 7436 882 Size: 2219 Fragments arranged by size: 2219 1990 955 865 778 617 546 424 379 BsaAI yAC'GTr Cuts at: 493 1204 3643 6564 493 Size: 711 2439 2921 2702 Fragments arranged by size: 2921 2702 2439 711 BsaBI GATnn'nnATC Cuts at: 8767 8767 Size: 8773 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5265 6580 6962 7597 Size: 53 83 186 936 3732 1315 382 635 Cuts at: 7597 275 Size: 1451 Fragments arranged by size: 3732 1451 1315 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4161 5924 6011 7206 7604 7878 8025 8436 4161 Size: 1763 87 1195 398 274 147 411 4498 Fragments arranged by size: 4498 1763 1195 411 398 274 147 87 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3383 3413 3612 3642 3689 Size: 30 165 30 1980 30 199 30 47 Cuts at: 3689 3719 3909 3939 4931 4961 1178 Size: 30 190 30 992 30 4990 Fragments arranged by size: 4990 1980 992 199 190 165 47 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3633 4804 4955 5627 5630 6054 1186 Size: 2447 1171 151 672 3 424 3905 Fragments arranged by size: 3905 2447 1171 672 424 151 3 BseYI C'CCAG_C Cuts at: 2567 3413 5864 7976 8472 2567 Size: 846 2451 2112 496 2868 Fragments arranged by size: 2868 2451 2112 846 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2260 2946 1163 Size: 573 524 686 6990 Fragments arranged by size: 6990 686 573 524 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6223 6984 8012 1540 Size: 275 186 4222 761 1028 2301 Fragments arranged by size: 4222 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2876 3719 4648 4919 6062 6834 6919 7990 2876 Size: 843 929 271 1143 772 85 1071 3659 Fragments arranged by size: 3659 1143 1071 929 843 772 271 85 BsmI GAATG_Cn' Cuts at: 3847 3901 6284 6377 3847 Size: 54 2383 93 6243 Fragments arranged by size: 6243 2383 93 54 BsmBI CGTCTCn'nnnn_ Cuts at: 2700 2700 Size: 8773 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2876 3414 3719 4276 4648 4834 4919 Size: 399 1086 538 305 557 372 186 85 Cuts at: 4919 5386 5445 5865 6062 6092 6834 6919 7990 Size: 467 59 420 197 30 742 85 1071 Cuts at: 7990 1391 Size: 2174 Fragments arranged by size: 2174 1086 1071 742 557 538 467 420 399 372 305 197 186 85 85 59 30 BspEI T'CCGG_A Cuts at: 5924 8436 5924 Size: 2512 6261 BspHI T'CATG_A Cuts at: 6662 8392 6662 Size: 1730 7043 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4220 6128 844 Size: 3376 1908 3489 Fragments arranged by size: 3489 3376 1908 BsrBI CCG'CTC Cuts at: 1394 1823 6660 1394 Size: 429 4837 3507 Fragments arranged by size: 4837 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3961 7268 7442 65 Size: 1183 2713 3307 174 1396 Fragments arranged by size: 3307 2713 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4500 5697 6011 7417 7604 1700 Size: 110 2690 1197 314 1406 187 2869 Fragments arranged by size: 2869 2690 1406 1197 314 187 110 BsrGI T'GTAC_A Cuts at: 96 2412 2643 2741 2916 3585 5247 96 Size: 2316 231 98 175 669 1662 3622 Fragments arranged by size: 3622 2316 1662 669 231 175 98 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8773 BssSI C'ACGA_G Cuts at: 6833 7845 6833 Size: 1012 7761 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8773 BstBI TT'CG_AA Cuts at: 2798 7577 2798 Size: 4779 3994 BstEII G'GTnAC_C Cuts at: 1184 3071 4682 1184 Size: 1887 1611 5275 Fragments arranged by size: 5275 1887 1611 BstXI CCAn_nnnn'nTGG Cuts at: 4420 4420 Size: 8773 BstYI r'GATC_y Cuts at: 1972 2369 3728 4423 4665 4876 4984 5170 6103 Size: 397 1359 695 242 211 108 186 933 Cuts at: 6103 6470 6855 6872 8324 8410 8422 8768 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1359 933 695 397 385 367 346 242 211 186 108 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4787 5305 4787 Size: 518 8255 BtgI C'CryG_G Cuts at: 513 1065 3030 4528 513 Size: 552 1965 1498 4758 Fragments arranged by size: 4758 1965 1498 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2259 5049 487 Size: 683 56 1033 2790 4211 Fragments arranged by size: 4211 2790 1033 683 56 BtsI GCAGTG_nn' Cuts at: 2218 3717 4231 6289 7083 7103 2218 Size: 1499 514 2058 794 20 3888 Fragments arranged by size: 3888 2058 1499 794 514 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8773 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3362 3397 3768 3803 4854 4889 529 Size: 35 2798 35 371 35 1051 35 4413 Fragments arranged by size: 4413 2798 1051 371 35 35 35 35 DraI TTT'AAA Cuts at: 4108 5985 6077 4108 Size: 1877 92 6804 Fragments arranged by size: 6804 1877 92 DraIII CAC_nnn'GTG Cuts at: 2489 4634 2489 Size: 2145 6628 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7780 809 Size: 507 6464 1802 Fragments arranged by size: 6464 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2025 4525 4540 Size: 54 1425 50 275 18 195 2500 15 Cuts at: 4540 6220 7109 8738 8 Size: 1680 889 1629 43 Fragments arranged by size: 2500 1680 1629 1425 889 275 195 54 50 43 18 15 EagI C'GGCC_G Cuts at: 1537 1812 6220 1537 Size: 275 4408 4090 Fragments arranged by size: 4408 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2154 2258 3390 4962 5559 5799 6703 1353 Size: 801 104 1132 1572 597 240 904 3423 Fragments arranged by size: 3423 1572 1132 904 801 597 240 104 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7360 7734 7880 387 Size: 1064 138 5771 374 146 1280 Fragments arranged by size: 5771 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4646 4917 6060 4646 Size: 271 1143 7359 Fragments arranged by size: 7359 1143 271 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2319 4043 4415 4526 5001 5913 5947 Size: 393 687 1724 372 111 475 912 34 Cuts at: 5947 6017 6836 7433 8220 8488 1239 Size: 70 819 597 787 268 1524 Fragments arranged by size: 1724 1524 912 819 787 687 597 475 393 372 268 111 70 34 EcoO109I rG'GnC_Cy Cuts at: 3653 5086 5382 5383 3653 Size: 1433 296 1 7043 Fragments arranged by size: 7043 1433 296 1 EcoRI G'AATT_C Cuts at: 4487 4754 6082 6460 4487 Size: 267 1328 378 6800 Fragments arranged by size: 6800 1328 378 267 EcoRV GAT'ATC Cuts at: 5544 5728 5930 5544 Size: 184 202 8387 Fragments arranged by size: 8387 202 184 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2141 2572 2749 4306 5996 2141 Size: 431 177 1557 1690 4918 Fragments arranged by size: 4918 1690 1557 431 177 FspI TGC'GCA Cuts at: 7279 7279 Size: 8773 HaeII r_GCGC'y Cuts at: 1678 4891 7920 1678 Size: 3213 3029 2531 Fragments arranged by size: 3213 3029 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2321 2353 2354 2386 2437 Size: 32 135 32 749 32 1 32 51 Cuts at: 2437 2469 3637 3669 3689 3721 3909 3941 4931 Size: 32 1168 32 20 32 188 32 990 Cuts at: 4931 4963 5255 5287 7414 7446 7488 7520 1373 Size: 32 292 32 2127 32 42 32 2626 Fragments arranged by size: 2626 2127 1168 990 749 292 188 135 51 42 32 32 32 32 32 32 32 32 32 32 32 32 20 1 HincII GTy'rAC Cuts at: 669 1657 1938 3604 4702 6117 6363 669 Size: 988 281 1666 1098 1415 246 3079 Fragments arranged by size: 3079 1666 1415 1098 988 281 246 HindIII A'AGCT_T Cuts at: 748 2726 3758 748 Size: 1978 1032 5763 Fragments arranged by size: 5763 1978 1032 HpaI GTT'AAC Cuts at: 6363 6363 Size: 8773 KpnI G_GTAC'C Cuts at: 6098 6098 Size: 8773 MfeI C'AATT_G Cuts at: 6372 6372 Size: 8773 MluI A'CGCG_T Cuts at: 8649 8649 Size: 8773 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2698 3369 4840 7887 8071 1305 Size: 1393 671 1471 3047 184 2007 Fragments arranged by size: 3047 2007 1471 1393 671 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 4542 10 Size: 54 1425 343 2710 4241 Fragments arranged by size: 4241 2710 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4319 4418 4692 5937 5954 6731 7090 Size: 648 3153 99 274 1245 17 777 359 Cuts at: 7090 7249 518 Size: 159 2042 Fragments arranged by size: 3153 2042 1245 777 648 359 274 159 99 17 MspA1I CmG'CkG Cuts at: 1606 6189 6866 8014 8259 1606 Size: 4583 677 1148 245 2120 Fragments arranged by size: 4583 2120 1148 677 245 NaeI GCC'GGC Cuts at: 5699 5699 Size: 8773 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 8221 NdeI CA'TA_TG Cuts at: 387 387 Size: 8773 NgoMIV G'CCGG_C Cuts at: 5697 5697 Size: 8773 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8773 NotI GC'GGCC_GC Cuts at: 6220 6220 Size: 8773 NruI TCG'CGA Cuts at: 5068 5068 Size: 8773 NsiI A_TGCA'T Cuts at: 3901 3901 Size: 8773 NspI r_CATG'y Cuts at: 1132 1229 3149 3339 3682 4224 6131 7676 1132 Size: 97 1920 190 343 542 1907 1545 2229 Fragments arranged by size: 2229 1920 1907 1545 542 343 190 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8773 PciI A'CATG_T Cuts at: 3335 7672 3335 Size: 4337 4436 PflMI CCAn_nnn'nTGG Cuts at: 1108 4482 5324 5849 1108 Size: 3374 842 525 4032 Fragments arranged by size: 4032 3374 842 525 PmeI GTTT'AAAC Cuts at: 6077 6077 Size: 8773 PmlI CAC'GTG Cuts at: 3643 3643 Size: 8773 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6842 6874 8355 8387 6842 Size: 32 1481 32 7228 Fragments arranged by size: 7228 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3653 5086 3653 Size: 1433 7340 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8773 PsiI TTA'TAA Cuts at: 5166 6343 5166 Size: 1177 7596 PspOMI G'GGCC_C Cuts at: 5382 5382 Size: 8773 PstI C_TGCA'G Cuts at: 830 3724 5679 6125 830 Size: 2894 1955 446 3478 Fragments arranged by size: 3478 2894 1955 446 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8773 RsrII CG'GwC_CG Cuts at: 4365 4365 Size: 8773 SacI G_AGCT'C Cuts at: 4648 4919 6062 4648 Size: 271 1143 7359 Fragments arranged by size: 7359 1143 271 SalI G'TCGA_C Cuts at: 1936 6115 1936 Size: 4179 4594 SapI GCTCTTCn'nnn_ Cuts at: 5799 5799 Size: 8773 SbfI CC_TGCA'GG Cuts at: 6125 6125 Size: 8773 ScaI AGT'ACT Cuts at: 7021 8733 7021 Size: 1712 7061 SfcI C'TryA_G Cuts at: 826 1045 2883 3720 5675 6121 7256 7937 8128 Size: 219 1838 837 1955 446 1135 681 191 Cuts at: 8128 826 Size: 1471 Fragments arranged by size: 1955 1838 1471 1135 837 681 446 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8773 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8773 SmaI CCC'GGG Cuts at: 1872 2057 5156 6100 1872 Size: 185 3099 944 4545 Fragments arranged by size: 4545 3099 944 185 SmlI C'TyrA_G Cuts at: 820 1017 1943 3116 3890 3936 4661 6877 7778 Size: 197 926 1173 774 46 725 2216 901 Cuts at: 7778 8040 820 Size: 262 1553 Fragments arranged by size: 2216 1553 1173 926 901 774 725 262 197 46 SnaBI TAC'GTA Cuts at: 493 6564 493 Size: 6071 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8773 SphI G_CATG'C Cuts at: 1132 4224 6131 1132 Size: 3092 1907 3774 Fragments arranged by size: 3774 3092 1907 SspI AAT'ATT Cuts at: 5 52 5298 6697 5 Size: 47 5246 1399 2081 Fragments arranged by size: 5246 2081 1399 47 StuI AGG'CCT Cuts at: 1517 4203 1517 Size: 2686 6087 StyI C'CwwG_G Cuts at: 513 1065 2908 3067 3913 5007 6212 513 Size: 552 1843 159 846 1094 1205 3074 Fragments arranged by size: 3074 1843 1205 1094 846 552 159 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2329 4018 6800 6817 6970 7155 7600 Size: 1255 749 1689 2782 17 153 185 445 Cuts at: 7600 325 Size: 1498 Fragments arranged by size: 2782 1689 1498 1255 749 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2412 2643 2741 Size: 274 80 33 51 1443 435 231 98 Cuts at: 2741 2916 3148 3585 5247 5958 7019 8731 96 Size: 175 232 437 1662 711 1061 1712 138 Fragments arranged by size: 1712 1662 1443 1061 711 437 435 274 232 231 175 138 98 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3383 3415 3612 3644 5088 5120 8733 8765 3383 Size: 32 197 32 1444 32 3613 32 3391 Fragments arranged by size: 3613 3391 1444 197 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3923 1316 Size: 2607 6166 XbaI T'CTAG_A Cuts at: 6109 6109 Size: 8773 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3090 4098 5780 3090 Size: 1008 1682 6083 Fragments arranged by size: 6083 1682 1008 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8773 XmaI C'CCGG_G Cuts at: 1870 2055 5154 6098 1870 Size: 185 3099 944 4545 Fragments arranged by size: 4545 3099 944 185 XmnI GAAnn'nnTTC Cuts at: 6902 6902 Size: 8773 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5266 6581 276 Size: 53 83 186 936 3732 1315 2468 Fragments arranged by size: 3732 2468 1315 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI RsrII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI AvrII BaeI BbeI BbvCI BsiWI BstZ17I EcoNI FseI FspAI KasI NarI PacI PfoI PspXI PsrI PvuII SacII SanDI SexAI SfiI SfoI SrfI SwaI