(Circular) (Six-base) MAPSORT of: pFN21AE6166.seq Check: 5153 from: 1 to: 4945 pFN21AE6166 4945 bp With 182 enzymes: SgfI * December 8, 2014 13:53 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2755 278 Size: 53 83 186 936 1219 2468 Fragments arranged by size: 2468 1219 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2288 1937 Size: 351 4594 Acc65I G'GTAC_C Cuts at: 2266 2266 Size: 4945 AclI AA'CG_TT Cuts at: 1563 3072 3445 1563 Size: 1509 373 3063 Fragments arranged by size: 3063 1509 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3008 4392 4660 1632 Size: 1376 1384 268 1917 Fragments arranged by size: 1917 1384 1376 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4748 AflIII A'CryG_T Cuts at: 1203 3844 4821 1203 Size: 2641 977 1327 Fragments arranged by size: 2641 1327 977 AgeI A'CCGG_T Cuts at: 3776 3776 Size: 4945 AhdI GACnn_n'nnGTC Cuts at: 3674 3674 Size: 4945 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4945 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4945 AlwNI CAG_nnn'CTG Cuts at: 2688 4260 2688 Size: 1572 3373 ApaLI G'TGCA_C Cuts at: 3002 4158 3002 Size: 1156 3789 ApoI r'AATT_y Cuts at: 1493 2254 2480 2632 4741 1493 Size: 761 226 152 2109 1697 Fragments arranged by size: 2109 1697 761 226 152 AseI AT'TA_AT Cuts at: 160 1032 3499 160 Size: 872 2467 1606 Fragments arranged by size: 2467 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4945 AvaI C'yCGr_G Cuts at: 1870 1943 2270 1870 Size: 73 327 4545 Fragments arranged by size: 4545 327 73 BamHI G'GATC_C Cuts at: 2275 2642 2275 Size: 367 4578 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2266 3721 618 Size: 325 217 627 479 1455 1842 Fragments arranged by size: 1842 1455 627 479 325 217 BanII G_rGCy'C Cuts at: 1391 2264 1391 Size: 873 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3974 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3136 3170 1903 Size: 34 1199 34 3678 Fragments arranged by size: 3678 1199 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2837 4047 2837 Size: 1210 3735 BclI T'GATC_A Cuts at: 1548 1548 Size: 4945 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3556 136 Size: 107 122 71 631 605 1884 1525 Fragments arranged by size: 1884 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4940 4940 Size: 4945 BlpI GC'TnA_GC Cuts at: 2362 2362 Size: 4945 Bme1580I G_kGCm'C Cuts at: 1790 3006 4162 1790 Size: 1216 1156 2573 Fragments arranged by size: 2573 1216 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4945 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2676 3634 442 Size: 416 53 83 399 24 1259 958 1753 Fragments arranged by size: 1753 1259 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2132 1056 Size: 1076 3869 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3605 1239 Size: 2366 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2024 3070 3935 4233 2024 Size: 1046 865 298 2736 Fragments arranged by size: 2736 1046 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3608 882 Size: 617 2109 2219 Fragments arranged by size: 2219 2109 617 BsaAI yAC'GTr Cuts at: 493 1204 2736 493 Size: 711 1532 2702 Fragments arranged by size: 2702 1532 711 BsaBI GATnn'nnATC Cuts at: 4939 4939 Size: 4945 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2752 3134 3769 275 Size: 53 83 186 936 1219 382 635 1451 Fragments arranged by size: 1451 1219 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3378 3776 4050 4197 4608 3378 Size: 398 274 147 411 3715 Fragments arranged by size: 3715 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4720 Fragments arranged by size: 4720 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4945 BseYI C'CCAG_C Cuts at: 4148 4644 4148 Size: 496 4449 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4372 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2395 3156 4184 1540 Size: 275 186 394 761 1028 2301 Fragments arranged by size: 2301 1028 761 394 275 186 BsiHKAI G_wGCw'C Cuts at: 3006 3091 4162 3006 Size: 85 1071 3789 Fragments arranged by size: 3789 1071 85 BsmI GAATG_Cn' Cuts at: 2052 2456 2549 2052 Size: 404 93 4448 Fragments arranged by size: 4448 404 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2264 3006 3091 4162 1391 Size: 399 474 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 474 399 85 BspEI T'CCGG_A Cuts at: 4608 4608 Size: 4945 BspHI T'CATG_A Cuts at: 2104 2834 4564 2104 Size: 730 1730 2485 Fragments arranged by size: 2485 1730 730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2300 844 Size: 1456 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2832 1394 Size: 429 1009 3507 Fragments arranged by size: 3507 1009 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3440 3614 65 Size: 1183 2192 174 1396 Fragments arranged by size: 2192 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3589 3776 1700 Size: 110 1779 187 2869 Fragments arranged by size: 2869 1779 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4945 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4945 BssSI C'ACGA_G Cuts at: 3005 4017 3005 Size: 1012 3933 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4945 BstBI TT'CG_AA Cuts at: 3749 3749 Size: 4945 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4945 BstXI CCAn_nnnn'nTGG Cuts at: 2232 2232 Size: 4945 BstYI r'GATC_y Cuts at: 1972 2275 2642 3027 3044 4496 4582 4594 4940 Size: 303 367 385 17 1452 86 12 346 Cuts at: 4940 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 303 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4393 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4206 Fragments arranged by size: 4206 683 56 BtsI GCAGTG_nn' Cuts at: 2461 3255 3275 2461 Size: 794 20 4131 Fragments arranged by size: 4131 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4945 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2164 2199 529 Size: 35 1600 35 3275 Fragments arranged by size: 3275 1600 35 35 DraI TTT'AAA Cuts at: 2249 2249 Size: 4945 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3952 809 Size: 507 2636 1802 Fragments arranged by size: 2636 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2016 2392 3281 Size: 54 1425 50 275 18 186 376 889 Cuts at: 3281 4910 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 376 275 186 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2392 1537 Size: 275 580 4090 Fragments arranged by size: 4090 580 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2875 1353 Size: 1522 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3532 3906 4052 387 Size: 1064 138 1943 374 146 1280 Fragments arranged by size: 1943 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3008 3605 4392 4660 1239 Size: 393 1376 597 787 268 1524 Fragments arranged by size: 1524 1376 787 597 393 268 EcoRI G'AATT_C Cuts at: 2254 2632 2254 Size: 378 4567 FspI TGC'GCA Cuts at: 3451 3451 Size: 4945 HaeII r_GCGC'y Cuts at: 1678 4092 1678 Size: 2414 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3586 3618 3660 3692 1373 Size: 32 135 32 2014 32 42 32 2626 Fragments arranged by size: 2626 2014 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2289 2535 669 Size: 988 281 351 246 3079 Fragments arranged by size: 3079 988 351 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 4945 HpaI GTT'AAC Cuts at: 2535 2535 Size: 4945 KpnI G_GTAC'C Cuts at: 2270 2270 Size: 4945 MfeI C'AATT_G Cuts at: 2544 2544 Size: 4945 MluI A'CGCG_T Cuts at: 4821 4821 Size: 4945 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4059 4243 1305 Size: 2754 184 2007 Fragments arranged by size: 2754 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2018 10 Size: 54 1425 343 186 2937 Fragments arranged by size: 2937 1425 343 186 54 MslI CAynn'nnrTG Cuts at: 518 1166 2079 2903 3262 3421 518 Size: 648 913 824 359 159 2042 Fragments arranged by size: 2042 913 824 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2361 3038 4186 4431 1606 Size: 755 677 1148 245 2120 Fragments arranged by size: 2120 1148 755 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4393 NdeI CA'TA_TG Cuts at: 387 387 Size: 4945 NheI G'CTAG_C Cuts at: 1052 2128 1052 Size: 1076 3869 NotI GC'GGCC_GC Cuts at: 2392 2392 Size: 4945 NspI r_CATG'y Cuts at: 1132 1229 2303 3848 1132 Size: 97 1074 1545 2229 Fragments arranged by size: 2229 1545 1074 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4945 PciI A'CATG_T Cuts at: 3844 3844 Size: 4945 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4945 PmeI GTTT'AAAC Cuts at: 2249 2249 Size: 4945 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3014 3046 4527 4559 3014 Size: 32 1481 32 3400 Fragments arranged by size: 3400 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4945 PsiI TTA'TAA Cuts at: 2515 2515 Size: 4945 PstI C_TGCA'G Cuts at: 830 2297 830 Size: 1467 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4945 SalI G'TCGA_C Cuts at: 1936 2287 1936 Size: 351 4594 SbfI CC_TGCA'GG Cuts at: 2297 2297 Size: 4945 ScaI AGT'ACT Cuts at: 3193 4905 3193 Size: 1712 3233 SfcI C'TryA_G Cuts at: 826 1045 2293 3428 4109 4300 826 Size: 219 1248 1135 681 191 1471 Fragments arranged by size: 1471 1248 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4945 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4945 SmaI CCC'GGG Cuts at: 1872 2272 1872 Size: 400 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2039 3049 3950 4212 820 Size: 197 926 96 1010 901 262 1553 Fragments arranged by size: 1553 1010 926 901 262 197 96 SnaBI TAC'GTA Cuts at: 493 2736 493 Size: 2243 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4945 SphI G_CATG'C Cuts at: 1132 2303 1132 Size: 1171 3774 SspI AAT'ATT Cuts at: 5 52 2869 5 Size: 47 2817 2081 Fragments arranged by size: 2817 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4945 StyI C'CwwG_G Cuts at: 513 1065 2013 2384 513 Size: 552 948 371 3074 Fragments arranged by size: 3074 948 552 371 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2152 2972 2989 3142 3327 3772 325 Size: 1255 572 820 17 153 185 445 1498 Fragments arranged by size: 1498 1255 820 572 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3191 4903 96 Size: 274 80 33 51 1443 1214 1712 138 Fragments arranged by size: 1712 1443 1214 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4905 4937 4905 Size: 32 4913 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4945 XbaI T'CTAG_A Cuts at: 2281 2281 Size: 4945 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 4945 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4945 XmaI C'CCGG_G Cuts at: 1870 2270 1870 Size: 400 4545 XmnI GAAnn'nnTTC Cuts at: 3074 3074 Size: 4945 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2753 276 Size: 53 83 186 936 1219 2468 Fragments arranged by size: 2468 1219 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI