Restriction map for pFN21AE6166
 (Circular) (Six-base) MAPSORT of: pFN21AE6166.seq  Check: 5153  from: 1  to: 4945



pFN21AE6166  4945 bp

 With 182 enzymes: SgfI * 

                        December 8, 2014 13:53  ..

AatII G_ACGT'C

Cuts at:    278     331     414     600    1536    2755     278 
   Size:         53      83     186     936    1219    2468

  Fragments arranged by size: 

                2468    1219     936     186      83      53
 
AccI GT'mk_AC

Cuts at:   1937    2288    1937 
   Size:        351    4594
 
Acc65I G'GTAC_C

Cuts at:   2266    2266 
   Size:       4945
 
AclI AA'CG_TT

Cuts at:   1563    3072    3445    1563 
   Size:       1509     373    3063

  Fragments arranged by size: 

                3063    1509     373
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:   1632    3008    4392    4660    1632 
   Size:       1376    1384     268    1917

  Fragments arranged by size: 

                1917    1384    1376     268
 
AflII C'TTAA_G

Cuts at:    820    1017     820 
   Size:        197    4748
 
AflIII A'CryG_T

Cuts at:   1203    3844    4821    1203 
   Size:       2641     977    1327

  Fragments arranged by size: 

                2641    1327     977
 
AgeI A'CCGG_T

Cuts at:   3776    3776 
   Size:       4945
 
AhdI GACnn_n'nnGTC

Cuts at:   3674    3674 
   Size:       4945
 
AleI CACnn'nnGTG

Cuts at:   1166    1166 
   Size:       4945
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1184    1184 
   Size:       4945
 
AlwNI CAG_nnn'CTG

Cuts at:   2688    4260    2688 
   Size:       1572    3373
 
ApaLI G'TGCA_C

Cuts at:   3002    4158    3002 
   Size:       1156    3789
 
ApoI r'AATT_y

Cuts at:   1493    2254    2480    2632    4741    1493 
   Size:        761     226     152    2109    1697

  Fragments arranged by size: 

                2109    1697     761     226     152
 
AseI AT'TA_AT

Cuts at:    160    1032    3499     160 
   Size:        872    2467    1606

  Fragments arranged by size: 

                2467    1606     872
 
AsiSI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       4945
 
AvaI C'yCGr_G

Cuts at:   1870    1943    2270    1870 
   Size:         73     327    4545

  Fragments arranged by size: 

                4545     327      73
 
BamHI G'GATC_C

Cuts at:   2275    2642    2275 
   Size:        367    4578
 
BanI G'GyrC_C

Cuts at:    618     943    1160    1787    2266    3721     618 
   Size:        325     217     627     479    1455    1842

  Fragments arranged by size: 

                1842    1455     627     479     325     217
 
BanII G_rGCy'C

Cuts at:   1391    2264    1391 
   Size:        873    4072
 
BbsI GAAGACnn'nnnn_

Cuts at:    928    1899     928 
   Size:        971    3974
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:   1903    1937    3136    3170    1903 
   Size:         34    1199      34    3678

  Fragments arranged by size: 

                3678    1199      34      34
 
BciVI GTATCCnnnnn_n'

Cuts at:   2837    4047    2837 
   Size:       1210    3735
 
BclI T'GATC_A

Cuts at:   1548    1548 
   Size:       4945
 
BglI GCCn_nnn'nGGC

Cuts at:    136     243     365     436    1067    1672    3556     136 
   Size:        107     122      71     631     605    1884    1525

  Fragments arranged by size: 

                1884    1525     631     605     122     107      71
 
BglII A'GATC_T

Cuts at:   4940    4940 
   Size:       4945
 
BlpI GC'TnA_GC

Cuts at:   2362    2362 
   Size:       4945
 
Bme1580I G_kGCm'C

Cuts at:   1790    3006    4162    1790 
   Size:       1216    1156    2573

  Fragments arranged by size: 

                2573    1216    1156
 
BmgBI CAC'GTC

Cuts at:   1318    1318 
   Size:       4945
 
BmrI ACTGGGnnnn_n'

Cuts at:    442     858     911     994    1393    1417    2676    3634     442 
   Size:        416      53      83     399      24    1259     958    1753

  Fragments arranged by size: 

                1753    1259     958     416     399      83      53      24
            
 
BmtI G_CTAG'C

Cuts at:   1056    2132    1056 
   Size:       1076    3869
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    3605    1239 
   Size:       2366    2579
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:   2024    3070    3935    4233    2024 
   Size:       1046     865     298    2736

  Fragments arranged by size: 

                2736    1046     865     298
 
BsaI GGTCTCn'nnnn_

Cuts at:    882    1499    3608     882 
   Size:        617    2109    2219

  Fragments arranged by size: 

                2219    2109     617
 
BsaAI yAC'GTr

Cuts at:    493    1204    2736     493 
   Size:        711    1532    2702

  Fragments arranged by size: 

                2702    1532     711
 
BsaBI GATnn'nnATC

Cuts at:   4939    4939 
   Size:       4945
 
BsaHI Gr'CG_yC

Cuts at:    275     328     411     597    1533    2752    3134    3769     275 
   Size:         53      83     186     936    1219     382     635    1451

  Fragments arranged by size: 

                1451    1219     936     635     382     186      83      53
            
 
BsaWI w'CCGG_w

Cuts at:   3378    3776    4050    4197    4608    3378 
   Size:        398     274     147     411    3715

  Fragments arranged by size: 

                3715     411     398     274     147
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:   1178    1208    1373    1403    1178 
   Size:         30     165      30    4720

  Fragments arranged by size: 

                4720     165      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:   1186    1186 
   Size:       4945
 
BseYI C'CCAG_C

Cuts at:   4148    4644    4148 
   Size:        496    4449
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:   1163    1736    1163 
   Size:        573    4372
 
BsiEI CG_ry'CG

Cuts at:   1540    1815    2001    2395    3156    4184    1540 
   Size:        275     186     394     761    1028    2301

  Fragments arranged by size: 

                2301    1028     761     394     275     186
 
BsiHKAI G_wGCw'C

Cuts at:   3006    3091    4162    3006 
   Size:         85    1071    3789

  Fragments arranged by size: 

                3789    1071      85
 
BsmI GAATG_Cn'

Cuts at:   2052    2456    2549    2052 
   Size:        404      93    4448

  Fragments arranged by size: 

                4448     404      93
 
Bsp1286I G_dGCh'C

Cuts at:   1391    1790    2264    3006    3091    4162    1391 
   Size:        399     474     742      85    1071    2174

  Fragments arranged by size: 

                2174    1071     742     474     399      85
 
BspEI T'CCGG_A

Cuts at:   4608    4608 
   Size:       4945
 
BspHI T'CATG_A

Cuts at:   2104    2834    4564    2104 
   Size:        730    1730    2485

  Fragments arranged by size: 

                2485    1730     730
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:    844    2300     844 
   Size:       1456    3489
 
BsrBI CCG'CTC

Cuts at:   1394    1823    2832    1394 
   Size:        429    1009    3507

  Fragments arranged by size: 

                3507    1009     429
 
BsrDI GCAATG_nn'

Cuts at:     65    1248    3440    3614      65 
   Size:       1183    2192     174    1396

  Fragments arranged by size: 

                2192    1396    1183     174
 
BsrFI r'CCGG_y

Cuts at:   1700    1810    3589    3776    1700 
   Size:        110    1779     187    2869

  Fragments arranged by size: 

                2869    1779     187     110
 
BsrGI T'GTAC_A

Cuts at:     96      96 
   Size:       4945
 
BssHII G'CGCG_C

Cuts at:   1925    1925 
   Size:       4945
 
BssSI C'ACGA_G

Cuts at:   3005    4017    3005 
   Size:       1012    3933
 
BstAPI GCAn_nnn'nTGC

Cuts at:    794     794 
   Size:       4945
 
BstBI TT'CG_AA

Cuts at:   3749    3749 
   Size:       4945
 
BstEII G'GTnAC_C

Cuts at:   1184    1184 
   Size:       4945
 
BstXI CCAn_nnnn'nTGG

Cuts at:   2232    2232 
   Size:       4945
 
BstYI r'GATC_y

Cuts at:   1972    2275    2642    3027    3044    4496    4582    4594    4940 
   Size:        303     367     385      17    1452      86      12     346

Cuts at:   4940    1972 
   Size:       1977

  Fragments arranged by size: 

                1977    1452     385     367     346     303      86      17
                  12
 
BtgI C'CryG_G

Cuts at:    513    1065     513 
   Size:        552    4393
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:    487    1170    1226     487 
   Size:        683      56    4206

  Fragments arranged by size: 

                4206     683      56
 
BtsI GCAGTG_nn'

Cuts at:   2461    3255    3275    2461 
   Size:        794      20    4131

  Fragments arranged by size: 

                4131     794      20
 
ClaI AT'CG_AT

Cuts at:   1554    1554 
   Size:       4945
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:    529     564    2164    2199     529 
   Size:         35    1600      35    3275

  Fragments arranged by size: 

                3275    1600      35      35
 
DraI TTT'AAA

Cuts at:   2249    2249 
   Size:       4945
 
DrdI GACnn_nn'nnGTC

Cuts at:    809    1316    3952     809 
   Size:        507    2636    1802

  Fragments arranged by size: 

                2636    1802     507
 
EaeI y'GGCC_r

Cuts at:      8      62    1487    1537    1812    1830    2016    2392    3281 
   Size:         54    1425      50     275      18     186     376     889

Cuts at:   3281    4910       8 
   Size:       1629      43

  Fragments arranged by size: 

                1629    1425     889     376     275     186      54      50
                  43      18
 
EagI C'GGCC_G

Cuts at:   1537    1812    2392    1537 
   Size:        275     580    4090

  Fragments arranged by size: 

                4090     580     275
 
EarI CTCTTCn'nnn_

Cuts at:   1353    2875    1353 
   Size:       1522    3423
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:    387    1451    1589    3532    3906    4052     387 
   Size:       1064     138    1943     374     146    1280

  Fragments arranged by size: 

                1943    1280    1064     374     146     138
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:   1239    1632    3008    3605    4392    4660    1239 
   Size:        393    1376     597     787     268    1524

  Fragments arranged by size: 

                1524    1376     787     597     393     268
 
EcoRI G'AATT_C

Cuts at:   2254    2632    2254 
   Size:        378    4567
 
FspI TGC'GCA

Cuts at:   3451    3451 
   Size:       4945
 
HaeII r_GCGC'y

Cuts at:   1678    4092    1678 
   Size:       2414    2531
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:   1373    1405    1540    1572    3586    3618    3660    3692    1373 
   Size:         32     135      32    2014      32      42      32    2626

  Fragments arranged by size: 

                2626    2014     135      42      32      32      32      32
            
 
HincII GTy'rAC

Cuts at:    669    1657    1938    2289    2535     669 
   Size:        988     281     351     246    3079

  Fragments arranged by size: 

                3079     988     351     281     246
 
HindIII A'AGCT_T

Cuts at:    748     748 
   Size:       4945
 
HpaI GTT'AAC

Cuts at:   2535    2535 
   Size:       4945
 
KpnI G_GTAC'C

Cuts at:   2270    2270 
   Size:       4945
 
MfeI C'AATT_G

Cuts at:   2544    2544 
   Size:       4945
 
MluI A'CGCG_T

Cuts at:   4821    4821 
   Size:       4945
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:   1305    4059    4243    1305 
   Size:       2754     184    2007

  Fragments arranged by size: 

                2754    2007     184
 
MscI TGG'CCA

Cuts at:     10      64    1489    1832    2018      10 
   Size:         54    1425     343     186    2937

  Fragments arranged by size: 

                2937    1425     343     186      54
 
MslI CAynn'nnrTG

Cuts at:    518    1166    2079    2903    3262    3421     518 
   Size:        648     913     824     359     159    2042

  Fragments arranged by size: 

                2042     913     824     648     359     159
 
MspA1I CmG'CkG

Cuts at:   1606    2361    3038    4186    4431    1606 
   Size:        755     677    1148     245    2120

  Fragments arranged by size: 

                2120    1148     755     677     245
 
NcoI C'CATG_G

Cuts at:    513    1065     513 
   Size:        552    4393
 
NdeI CA'TA_TG

Cuts at:    387     387 
   Size:       4945
 
NheI G'CTAG_C

Cuts at:   1052    2128    1052 
   Size:       1076    3869
 
NotI GC'GGCC_GC

Cuts at:   2392    2392 
   Size:       4945
 
NspI r_CATG'y

Cuts at:   1132    1229    2303    3848    1132 
   Size:         97    1074    1545    2229

  Fragments arranged by size: 

                2229    1545    1074      97
 
PasI CC'CwG_GG

Cuts at:   1348    1348 
   Size:       4945
 
PciI A'CATG_T

Cuts at:   3844    3844 
   Size:       4945
 
PflMI CCAn_nnn'nTGG

Cuts at:   1108    1108 
   Size:       4945
 
PmeI GTTT'AAAC

Cuts at:   2249    2249 
   Size:       4945
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:   3014    3046    4527    4559    3014 
   Size:         32    1481      32    3400

  Fragments arranged by size: 

                3400    1481      32      32
 
PshAI GACnn'nnGTC

Cuts at:   1613    1613 
   Size:       4945
 
PsiI TTA'TAA

Cuts at:   2515    2515 
   Size:       4945
 
PstI C_TGCA'G

Cuts at:    830    2297     830 
   Size:       1467    3478
 
PvuI CG_AT'CG

Cuts at:   2001    2001 
   Size:       4945
 
SalI G'TCGA_C

Cuts at:   1936    2287    1936 
   Size:        351    4594
 
SbfI CC_TGCA'GG

Cuts at:   2297    2297 
   Size:       4945
 
ScaI AGT'ACT

Cuts at:   3193    4905    3193 
   Size:       1712    3233
 
SfcI C'TryA_G

Cuts at:    826    1045    2293    3428    4109    4300     826 
   Size:        219    1248    1135     681     191    1471

  Fragments arranged by size: 

                1471    1248    1135     681     219     191
 
SgfI GCG_AT'CGC

Cuts at:   2001    2001 
   Size:       4945
 
SgrAI Cr'CCGG_yG

Cuts at:   1700    1700 
   Size:       4945
 
SmaI CCC'GGG

Cuts at:   1872    2272    1872 
   Size:        400    4545
 
SmlI C'TyrA_G

Cuts at:    820    1017    1943    2039    3049    3950    4212     820 
   Size:        197     926      96    1010     901     262    1553

  Fragments arranged by size: 

                1553    1010     926     901     262     197      96
 
SnaBI TAC'GTA

Cuts at:    493    2736     493 
   Size:       2243    2702
 
SpeI A'CTAG_T

Cuts at:    152     152 
   Size:       4945
 
SphI G_CATG'C

Cuts at:   1132    2303    1132 
   Size:       1171    3774
 
SspI AAT'ATT

Cuts at:      5      52    2869       5 
   Size:         47    2817    2081

  Fragments arranged by size: 

                2817    2081      47
 
StuI AGG'CCT

Cuts at:   1517    1517 
   Size:       4945
 
StyI C'CwwG_G

Cuts at:    513    1065    2013    2384     513 
   Size:        552     948     371    3074

  Fragments arranged by size: 

                3074     948     552     371
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:    325    1580    2152    2972    2989    3142    3327    3772     325 
   Size:       1255     572     820      17     153     185     445    1498

  Fragments arranged by size: 

                1498    1255     820     572     445     185     153      17
            
 
TatI w'GTAC_w

Cuts at:     96     370     450     483     534    1977    3191    4903      96 
   Size:        274      80      33      51    1443    1214    1712     138

  Fragments arranged by size: 

                1712    1443    1214     274     138      80      51      33
            
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:   4905    4937    4905 
   Size:         32    4913
 
Tth111I GACn'n_nGTC

Cuts at:   1316    1316 
   Size:       4945
 
XbaI T'CTAG_A

Cuts at:   2281    2281 
   Size:       4945
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:   2011    2011 
   Size:       4945
 
XhoI C'TCGA_G

Cuts at:   1943    1943 
   Size:       4945
 
XmaI C'CCGG_G

Cuts at:   1870    2270    1870 
   Size:        400    4545
 
XmnI GAAnn'nnTTC

Cuts at:   3074    3074 
   Size:       4945
 
ZraI GAC'GTC

Cuts at:    276     329     412     598    1534    2753     276 
   Size:         53      83     186     936    1219    2468

  Fragments arranged by size: 

                2468    1219     936     186      83      53
 

 Enzymes that do cut:

    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI    AlwNI    ApaLI     ApoI     AseI    AsiSI
     AvaI    BamHI     BanI    BanII     BbsI     BcgI    BciVI     BclI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BpmI
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI
    BseYI     BsgI    BsiEI  BsiHKAI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI     BtgI    BtgZI     BtsI     ClaI
    CspCI     DraI     DrdI     EaeI     EagI     EarI     EciI  Eco57MI
    EcoRI     FspI    HaeII    Hin4I   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NcoI     NdeI
     NheI     NotI     NspI     PasI     PciI    PflMI     PmeI     PpiI
    PshAI     PsiI     PstI     PvuI     SalI     SbfI     ScaI     SfcI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SspI
     StuI     StyI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI

 Enzymes that do not cut: 

     AarI     AfeI     AloI     ApaI     AscI    AvrII     BaeI     BbeI
    BbvCI    BfrBI     BplI   Bpu10I    BsiWI    BsmBI  BstZ17I   Bsu36I
   DraIII  EcoICRI    EcoNI EcoO109I    EcoRV     FalI     FseI    FspAI
     KasI     NaeI     NarI   NgoMIV     NruI     NsiI     PacI     PfoI
     PmlI    PpuMI   PspOMI    PspXI     PsrI    PvuII    RsrII     SacI
    SacII    SanDI     SapI    SexAI     SfiI     SfoI     SrfI     SwaI
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