################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII AccII AccIII AcyI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BcnI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmBI BsoBI BspDI BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoT22I FauI FseI FspI HaeII HapII Hin1I HpaII KasI KspI MamI MluI MluNI MroI MscI MspI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI PstI PvuI PvuII RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SphI Sse8387I StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1980 GGTACC G^GTACC AclI 1087 AACGTT AA^CGTT AflIII 1727 ACATGT A^C[GA][CT]GT AhdI 561 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Asp718I 1980 GGTACC G^GTACC AspEI 561 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanII 2311 GGGCTC G[GA]GC[CT]^C BcgI 1196 CGAAGGATATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 378 AGATCT A^GATCT BlpI 193 GCTGAGC GC^T[ACGT]AGC BmyI 2311 GGGCTC G[AGT]GC[ACT]^C BpmI 1918 CTCCAG CTGGAG(16/14) Bpu1102I 193 GCTGAGC GC^T[ACGT]AGC BsgI 1269 CTGCAC GTGCAG(16/14) Bsp1286I 2311 GGGCTC G[AGT]GC[ACT]^C Bsp1407I 971 TGTACA T^GTACA BspLU11I 1727 ACATGT A^CATGT BsrGI 971 TGTACA T^GTACA Cac8I 1661 GCTGGC GC[ACGT]^[ACGT]GC CelII 193 GCTGAGC GC^T[ACGT]AGC CfoI 1875 GCGC GCG^C DraII 401 AGGCCCT [GA]G^G[ACGT]CC[CT] Eam1105I 561 GACAGAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 2079 GAAGAG CTCTTC(1/4) EcoO109I 401 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoRI 1236 GAATTC G^AATTC EcoRV 342 GATATC GAT^ATC EcoT14I 2291 CCAAGG C^C[AT][AT]GG HgaI 2265 GACGC GACGC(5/10) HhaI 1875 GCGC GCG^C HinP1I 1875 GCGC G^CGC HpaI 1005 GTTAAC GTT^AAC KpnI 1980 GGTACC GGTAC^C Ksp632I 2079 GAAGAG CTCTTC(1/4) MfeI 494 CAATTG C^AATTG MunI 494 CAATTG C^AATTG PshAI 697 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1087 AACGTT AA^CGTT SspBI 971 TGTACA T^GTACA StyI 2291 CCAAGG C^C[AT][AT]GG XcmI 1781 CCATGCTGGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AvaII 742 GGTCC G^G[AT]CC 1862 GGACC BbsI 1305 GTCTTC GAAGAC(2/6) 2015 GTCTTC BpuAI 1305 GTCTTC GAAGAC(2/6) 2015 GTCTTC BsaI 204 GGTCTC GGTCTC(1/5) 1186 GGTCTC BsmFI 1580 GGGAC GGGAC(10/14) 2035 GTCCC BstXI 824 CCAAACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1778 CCACCATGCTGG HincII 231 GTTGAC GT[CT]^[GA]AC 1005 GTTAAC HindII 231 GTTGAC GT[CT]^[GA]AC 1005 GTTAAC HindIII 30 AAGCTT A^AGCTT 1418 AAGCTT MspA1I 151 CCGCTG C[AC]G^C[GT]G 2269 CAGCGG MwoI 1475 GCAGCTTTAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1478 GCTTTAGCAGC NspI 1034 ACATGC [GA]CATG^[CT] 1727 ACATGT SfaNI 507 GCATC GCATC(5/9) 1817 GCATC SpeI 616 ACTAGT A^CTAGT 2358 ACTAGT ######################################### # 3 OR MORE CUT ENZYMES ( 62 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI ApoI Asp700I BanI BbvI BclI BfaI BsaJI BsiYI BslI BsmAI BsmI BspHI BsrI BssKI BstF5I BstNI BstYI Cfr13I DdeI DpnII EcoRII FbaI Fnu4HI FokI HaeIII HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MvaI NdeII NlaIII NlaIV PleI RcaI RsaI Sau3AI Sau96I ScrFI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII XmnI