Restriction map for pFN21AE3305
 (Linear) (Six-base) MAPSORT of: KIEE3305.seq  Check: 4352  from: 1  to: 2388



KIEE3305.orf

 With 182 enzymes: SgfI * 

                        December 27, 2011 16:35  ..

AccI GT'mk_AC

Cuts at:      0     107     570    1254    2388 
   Size:        107     463     684    1134

  Fragments arranged by size: 

                1134     684     463     107
 
Acc65I G'GTAC_C

Cuts at:      0    1980    2388 
   Size:       1980     408
 
AclI AA'CG_TT

Cuts at:      0    1088    2388 
   Size:       1088    1300
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184    2272    2388 
   Size:        184    2088     116

  Fragments arranged by size: 

                2088     184     116
 
AflIII A'CryG_T

Cuts at:      0    1727    2388 
   Size:       1727     661
 
AhdI GACnn_n'nnGTC

Cuts at:      0     566    2388 
   Size:        566    1822
 
ApoI r'AATT_y

Cuts at:      0      97     421     444     575     718     756    1236    1299 
   Size:         97     324      23     131     143      38     480      63

Cuts at:   1299    1615    2388 
   Size:        316     773

  Fragments arranged by size: 

                 773     480     324     316     143     131      97      63
                  38      23
 
BanI G'GyrC_C

Cuts at:      0    1774    1980    2132    2388 
   Size:       1774     206     152     256

  Fragments arranged by size: 

                1774     256     206     152
 
BanII G_rGCy'C

Cuts at:      0    2315    2388 
   Size:       2315      73
 
BbsI GAAGACnn'nnnn_

Cuts at:      0    1298    2008    2388 
   Size:       1298     710     380

  Fragments arranged by size: 

                1298     710     380
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0    1185    1219    2388 
   Size:       1185      34    1169

  Fragments arranged by size: 

                1185    1169      34
 
BclI T'GATC_A

Cuts at:      0      53    1499    1506    2388 
   Size:         53    1446       7     882

  Fragments arranged by size: 

                1446     882      53       7
 
BglII A'GATC_T

Cuts at:      0     378    2388 
   Size:        378    2010
 
BlpI GC'TnA_GC

Cuts at:      0     194    2388 
   Size:        194    2194
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1903    2388 
   Size:       1903     485
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     210    1192    2388 
   Size:        210     982    1196

  Fragments arranged by size: 

                1196     982     210
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1254    2388 
   Size:       1254    1134
 
BsmI GAATG_Cn'

Cuts at:      0     588     872    2228    2388 
   Size:        588     284    1356     160

  Fragments arranged by size: 

                1356     588     284     160
 
Bsp1286I G_dGCh'C

Cuts at:      0    2315    2388 
   Size:       2315      73
 
BspHI T'CATG_A

Cuts at:      0    1190    2019    2172    2261    2388 
   Size:       1190     829     153      89     127

  Fragments arranged by size: 

                1190     829     153     127      89
 
BsrGI T'GTAC_A

Cuts at:      0     971    2388 
   Size:        971    1417
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     831    1785    2388 
   Size:        831     954     603

  Fragments arranged by size: 

                 954     831     603
 
BstYI r'GATC_y

Cuts at:      0     378    1827    2179    2388 
   Size:        378    1449     352     209

  Fragments arranged by size: 

                1449     378     352     209
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     174    2388 
   Size:        174    2214
 
BtsI GCAGTG_nn'

Cuts at:      0    2290    2388 
   Size:       2290      98
 
EarI CTCTTCn'nnn_

Cuts at:      0    2074    2388 
   Size:       2074     314
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     184    1903    2272    2388 
   Size:        184    1719     369     116

  Fragments arranged by size: 

                1719     369     184     116
 
EcoO109I rG'GnC_Cy

Cuts at:      0     402    2388 
   Size:        402    1986
 
EcoRI G'AATT_C

Cuts at:      0    1236    2388 
   Size:       1236    1152
 
EcoRV GAT'ATC

Cuts at:      0     344    2388 
   Size:        344    2044
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0    2112    2388 
   Size:       2112     276
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     552     584    1491    1523    1604    1624    1636    1656 
   Size:        552      32     907      32      81      20      12      20

Cuts at:   1656    1854    1886    2368    2388 
   Size:        198      32     482      20

  Fragments arranged by size: 

                 907     552     482     198      81      32      32      32
                  20      20      20      12
 
HincII GTy'rAC

Cuts at:      0     233    1007    2388 
   Size:        233     774    1381

  Fragments arranged by size: 

                1381     774     233
 
HindIII A'AGCT_T

Cuts at:      0      30    1418    2388 
   Size:         30    1388     970

  Fragments arranged by size: 

                1388     970      30
 
HpaI GTT'AAC

Cuts at:      0    1007    2388 
   Size:       1007    1381
 
KpnI G_GTAC'C

Cuts at:      0    1984    2388 
   Size:       1984     404
 
MfeI C'AATT_G

Cuts at:      0     494    2388 
   Size:        494    1894
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      45     110     769    2388 
   Size:         45      65     659    1619

  Fragments arranged by size: 

                1619     659      65      45
 
MslI CAynn'nnrTG

Cuts at:      0    1000    1593    1786    2388 
   Size:       1000     593     193     602

  Fragments arranged by size: 

                1000     602     593     193
 
MspA1I CmG'CkG

Cuts at:      0     153    2271    2388 
   Size:        153    2118     117

  Fragments arranged by size: 

                2118     153     117
 
NspI r_CATG'y

Cuts at:      0    1038    1731    2388 
   Size:       1038     693     657

  Fragments arranged by size: 

                1038     693     657
 
PciI A'CATG_T

Cuts at:      0    1727    2388 
   Size:       1727     661
 
PfoI T'CCnGG_A

Cuts at:      0    1464    2388 
   Size:       1464     924
 
PshAI GACnn'nnGTC

Cuts at:      0     701    2388 
   Size:        701    1687
 
PsiI TTA'TAA

Cuts at:      0    1676    2388 
   Size:       1676     712
 
SpeI A'CTAG_T

Cuts at:      0     616    2358    2388 
   Size:        616    1742      30

  Fragments arranged by size: 

                1742     616      30
 
SspI AAT'ATT

Cuts at:      0     310     681     846    2388 
   Size:        310     371     165    1542

  Fragments arranged by size: 

                1542     371     310     165
 
StyI C'CwwG_G

Cuts at:      0    2291    2388 
   Size:       2291      97
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     626    1541    1953    2388 
   Size:        626     915     412     435

  Fragments arranged by size: 

                 915     626     435     412
 
TatI w'GTAC_w

Cuts at:      0     971    2388 
   Size:        971    1417
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     726     758    2388 
   Size:        726      32    1630

  Fragments arranged by size: 

                1630     726      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0    1788    2388 
   Size:       1788     600
 
XmnI GAAnn'nnTTC

Cuts at:      0     872    1090    2213    2388 
   Size:        872     218    1123     175

  Fragments arranged by size: 

                1123     872     218     175
 

 Enzymes that do cut:

     AccI   Acc65I     AclI     AcuI   AflIII     AhdI     ApoI     BanI
    BanII     BbsI     BcgI     BclI    BglII     BlpI     BpmI     BsaI
     BsgI     BsmI Bsp1286I    BspHI    BsrGI    BstXI    BstYI    BtgZI
     BtsI     EarI  Eco57MI EcoO109I    EcoRI    EcoRV     FalI    Hin4I
   HincII  HindIII     HpaI     KpnI     MfeI     MmeI     MslI   MspA1I
     NspI     PciI     PfoI    PshAI     PsiI     SpeI     SspI     StyI
    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AfeI    AflII     AgeI     AleI     AlfI     AloI
    AlwNI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI    BamHI     BbeI    BbvCI    BciVI    BfrBI     BglI Bme1580I
    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI
    BsaHI    BsaWI    BsaXI    BseRI    BseYI    BsiEI  BsiHKAI    BsiWI
    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI   BssHII    BssSI
   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
     FseI     FspI    FspAI    HaeII     KasI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PmeI     PmlI     PpiI    PpuMI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII
     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SphI     SrfI
     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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