################################## # NO CUT ENZYMES ( 140 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflIII AgeI AhdI AlwI AlwNI Aor51HI ApaI AscI Asp718I AspEI AspI AviII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BclI BglI BglII BlpI BpmI Bpu1102I BsaBI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI BstUI BstXI BstYI CelII CfoI Cfr10I ClaI CpoI DraII DraIII EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoO109I EcoO65I EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HindIII HpaI KasI KpnI KspI MamI MfeI MflI MluI MluNI MroI MscI MunI MvnI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI XcmI XhoI XhoII ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccI 274 GTATAC GT^[AC][GT]AC AflII 838 CTTAAG C^TTAAG Alw44I 336 GTGCAC G^TGCAC ApaLI 336 GTGCAC G^TGCAC AspHI 336 GTGCAC G[AT]GC[AT]^C AvaI 971 CCCGGG C^[CT]CG[GA]G AvaII 18 GGACC G^G[AT]CC AvrII 1404 CCTAGG C^CTAGG BbsI 104 GTCTTC GAAGAC(2/6) BfrI 838 CTTAAG C^TTAAG BlnI 1404 CCTAGG C^CTAGG BpuAI 104 GTCTTC GAAGAC(2/6) BsaAI 686 TACGTA [CT]AC^GT[GA] BsaI 111 GAGACC GGTCTC(1/5) BseRI 10 GAGGAG GAGGAG(10/8) BsgI 242 CTGCAC GTGCAG(16/14) BsiHKAI 336 GTGCAC G[AT]GC[AT]^C BsmAI 111 GAGAC GTCTC(1/5) BsmFI 755 GTCCC GGGAC(10/14) BsoBI 971 CCCGGG C^[CT]CG[GA]G BsrDI 911 GCAATG GCAATG(2/0) Bst1107I 274 GTATAC GTA^TAC BstAPI 1507 GCAGTTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstNI 95 CCTGG CC^[AT]GG BstZ17I 274 GTATAC GTA^TAC DpnII 1375 GATC ^GATC DrdI 746 GACAAGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 758 CCACGG C^C[GA][CT]GG EcoNI 1473 CCTGATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 154 GAATTC G^AATTC EcoRII 95 CCTGG ^CC[AT]GG EcoT14I 1404 CCTAGG C^C[AT][AT]GG HapII 972 CCGG C^CGG HincII 900 GTTGAC GT[CT]^[GA]AC HindII 900 GTTGAC GT[CT]^[GA]AC HpaII 972 CCGG C^CGG MaeII 687 ACGT A^CGT MboI 1375 GATC ^GATC MslI 1331 CATTTAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 972 CCGG C^CGG MvaI 95 CCTGG CC^[AT]GG NdeII 1375 GATC ^GATC NlaIV 61 GGCTCC GG[ACGT]^[ACGT]CC NspI 681 GCATGT [GA]CATG^[CT] PacI 484 TTAATTAA TTAAT^TAA PflMI 1075 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 1496 CTGCAG CTGCA^G Sau3AI 1375 GATC ^GATC SmaI 971 CCCGGG CCC^GGG SnaBI 686 TACGTA TAC^GTA SspI 796 AATATT AAT^ATT StuI 92 AGGCCT AGG^CCT StyI 1404 CCTAGG C^C[AT][AT]GG TaqI 1347 TCGA T^CGA TthHB8I 1347 TCGA T^CGA Van91I 1075 CCACCAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 971 CCCGGG C^CCGGG XmaI 971 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### AocI 463 CCTAAGG CC^T[ACGT]AGG 811 CCTGAGG AseI 483 ATTAAT AT^TAAT 487 ATTAAT AsnI 483 ATTAAT AT^TAAT 487 ATTAAT Asp700I 1255 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1504 GAAGCAGTTC BcnI 971 CCCGG CC^[GC]GG 972 CCGGG Bsu36I 463 CCTAAGG CC^T[ACGT]AGG 811 CCTGAGG Cfr13I 18 GGACC G^G[ACGT]CC 98 GGGCC Eco81I 463 CCTAAGG CC^T[ACGT]AGG 811 CCTGAGG NciI 971 CCCGG CC^[GC]GG 972 CCGGG PshBI 483 ATTAAT AT^TAAT 487 ATTAAT Sau96I 18 GGACC G^G[ACGT]CC 98 GGGCC SfcI 1370 CTACAG C^T[GA][CT]AG 1496 CTGCAG XmnI 1255 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1504 GAAGCAGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AcsI AfaI AluI ApoI BanII BbvI BciVI BfaI BmyI BsaJI BsiYI BslI BsmI Bsp1286I BsrI BssKI BstF5I Cac8I DdeI DraI EarI Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeIII MboII MnlI MseI MspA1I MwoI NlaIII PleI PvuII RsaI ScrFI SmlI TfiI Tru9I TseI Tsp45I Tsp509I TspRI