Restriction map for
pFN21AE9437
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# NO CUT ENZYMES ( 132 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AlwI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvaII AviII
AvrII BalI BamHI BbrPI BciVI
BclI BfaI BfrI BglI BglII
BlnI BsaBI BsaI BsaWI BseAI
BseRI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstYI BstZ17I ClaI CpoI
DpnII DraI DrdI Eam1105I EcoNI
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MaeI
MamI MboI MfeI MflI MluI
MluNI MroI MscI MseI MunI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspV PacI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI Sau3AI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI Sse8387I SspBI
SspI StuI StyI SwaI Tru9I
Tsp509I XbaI XcmI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AocI 119 CCTCAGG CC^T[ACGT]AGG
ApaI 3 GGGCCC GGGCC^C
BbsI 29 GTCTTC GAAGAC(2/6)
BbvCI 831 CCTCAGC CCTCAGC(-5/-2)
BcgI 449 GCAGCGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 577 CTCCAG CTGGAG(16/14)
BpuAI 29 GTCTTC GAAGAC(2/6)
BsaAI 876 CACGTA [CT]AC^GT[GA]
BsmFI 393 GGGAC GGGAC(10/14)
BspHI 773 TCATGA T^CATGA
BsrDI 732 CATTGC GCAATG(2/0)
Bsu36I 119 CCTCAGG CC^T[ACGT]AGG
DraII 3 GGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 586 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 895 CGGCCG C^GGCCG
EclXI 895 CGGCCG C^GGCCG
Eco52I 895 CGGCCG C^GGCCG
Eco81I 119 CCTCAGG CC^T[ACGT]AGG
EcoO109I 3 GGGCCCC [GA]G^G[ACGT]CC[CT]
MaeII 877 ACGT A^CGT
MslI 661 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 113 CTCGAG C^TCGAG
PstI 1075 CTGCAG CTGCA^G
PvuII 1003 CAGCTG CAG^CTG
RcaI 773 TCATGA T^CATGA
SmlI 113 CTCGAG C^T[CT][GA]AG
SphI 232 GCATGC GCATG^C
TaqI 114 TCGA T^CGA
TthHB8I 114 TCGA T^CGA
XhoI 113 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
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AfaI 43 GTAC GT^AC
879 GTAC
Aor51HI 97 AGCGCT AGC^GCT
677 AGCGCT
AspI 213 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
619 GACACCGTC
AvaI 113 CTCGAG C^[CT]CG[GA]G
484 CCCGAG
BanI 222 GGCGCC G^G[CT][GA]CC
701 GGCGCC
BbeI 222 GGCGCC GGCGC^C
701 GGCGCC
BsgI 419 CTGCAC GTGCAG(16/14)
658 CTGCAC
BsiEI 355 CGACCG CG[GA][CT]^CG
895 CGGCCG
BsoBI 113 CTCGAG C^[CT]CG[GA]G
484 CCCGAG
BspMI 155 ACCTGC ACCTGC(4/8)
656 ACCTGC
BssSI 432 CTCGTG CACGAG(-5/-1)
793 CTCGTG
BstXI 65 CCAACGCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
237 CCAAGTCATTGG
DsaI 50 CCGTGG C^C[GA][CT]GG
533 CCGTGG
EarI 939 GAAGAG CTCTTC(1/4)
1011 GAAGAG
Eco47III 97 AGCGCT AGC^GCT
677 AGCGCT
HinfI 138 GAATC G^A[ACGT]TC
1066 GATTC
KasI 222 GGCGCC G^GCGCC
701 GGCGCC
Ksp632I 939 GAAGAG CTCTTC(1/4)
1011 GAAGAG
MaeIII 625 GTCAC ^GT[ACGT]AC
805 GTCAC
MspA1I 550 CCGCTG C[AC]G^C[GT]G
1003 CAGCTG
NarI 222 GGCGCC GG^CGCC
701 GGCGCC
NspI 176 ACATGC [GA]CATG^[CT]
232 GCATGC
PflFI 213 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
619 GACACCGTC
RsaI 43 GTAC GT^AC
879 GTAC
SacI 103 GAGCTC GAGCT^C
790 GAGCTC
SfoI 222 GGCGCC GGC^GCC
701 GGCGCC
TfiI 138 GAATC G^A[AT]TC
1066 GATTC
Tsp45I 625 GTCAC ^GT[GC]AC
805 GTCAC
Tth111I 213 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
619 GACACCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AluI Alw44I
AlwNI ApaLI AspHI BanII BbvI
BcnI BlpI BmyI Bpu1102I BsaHI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BsrFI BsrI BssKI BstAPI BstF5I
BstNI BstUI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI EaeI EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI MboII MnlI
MspI MvaI MvnI MwoI NaeI
NciI NgoMIV NlaIII NlaIV PflMI
Sau96I ScrFI SfaNI SfcI TseI
TspRI Van91I