(Circular) (Six-base) MAPSORT of: pFN21AE9437.seq Check: 7349 from: 1 to: 5794 pFN21AE9437 5794 bp With 182 enzymes: SgfI * December 28, 2011 15:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2169 2169 Size: 5794 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3604 278 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3137 1937 Size: 1200 4594 Acc65I G'GTAC_C Cuts at: 3115 3115 Size: 5794 AclI AA'CG_TT Cuts at: 1563 3921 4294 1563 Size: 2358 373 3063 Fragments arranged by size: 3063 2358 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2963 3035 3857 5241 5509 1632 Size: 1331 72 822 1384 268 1917 Fragments arranged by size: 1917 1384 1331 822 268 72 AfeI AGC'GCT Cuts at: 2104 2684 2104 Size: 580 5214 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5597 AflIII A'CryG_T Cuts at: 1203 3091 4693 5670 1203 Size: 1888 1602 977 1327 Fragments arranged by size: 1888 1602 1327 977 AgeI A'CCGG_T Cuts at: 4625 4625 Size: 5794 AhdI GACnn_n'nnGTC Cuts at: 4523 4523 Size: 5794 AleI CACnn'nnGTG Cuts at: 1166 2670 1166 Size: 1504 4290 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2449 1184 Size: 1265 4529 AlwNI CAG_nnn'CTG Cuts at: 2320 2407 2423 2610 3013 3537 5109 2320 Size: 87 16 187 403 524 1572 3005 Fragments arranged by size: 3005 1572 524 403 187 87 16 ApaI G_GGCC'C Cuts at: 2012 2012 Size: 5794 ApaLI G'TGCA_C Cuts at: 2588 2930 3002 3851 5007 2588 Size: 342 72 849 1156 3375 Fragments arranged by size: 3375 1156 849 342 72 ApoI r'AATT_y Cuts at: 1493 3103 3329 3481 5590 1493 Size: 1610 226 152 2109 1697 Fragments arranged by size: 2109 1697 1610 226 152 AseI AT'TA_AT Cuts at: 160 1032 4348 160 Size: 872 3316 1606 Fragments arranged by size: 3316 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 AvaI C'yCGr_G Cuts at: 1870 1943 2118 2489 3119 1870 Size: 73 175 371 630 4545 Fragments arranged by size: 4545 630 371 175 73 BamHI G'GATC_C Cuts at: 3124 3491 3124 Size: 367 5427 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2227 2706 3115 4570 618 Size: 325 217 627 440 479 409 1455 1842 Fragments arranged by size: 1842 1455 627 479 440 409 325 217 BanII G_rGCy'C Cuts at: 1391 2012 2112 2799 3113 1391 Size: 621 100 687 314 4072 Fragments arranged by size: 4072 687 621 314 100 BbeI G_GCGC'C Cuts at: 2231 2710 2231 Size: 479 5315 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2027 928 Size: 971 128 4695 Fragments arranged by size: 4695 971 128 BbvCI CC'TCA_GC Cuts at: 2837 2837 Size: 5794 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2443 2477 3985 4019 1903 Size: 34 506 34 1508 34 3678 Fragments arranged by size: 3678 1508 506 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3686 4896 3686 Size: 1210 4584 BclI T'GATC_A Cuts at: 1548 1548 Size: 5794 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4405 136 Size: 107 122 71 631 605 2733 1525 Fragments arranged by size: 2733 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5789 5789 Size: 5794 BlpI GC'TnA_GC Cuts at: 2106 2288 2303 3211 2106 Size: 182 15 908 4689 Fragments arranged by size: 4689 908 182 15 Bme1580I G_kGCm'C Cuts at: 1790 2012 2592 2805 2934 3006 3855 5011 1790 Size: 222 580 213 129 72 849 1156 2573 Fragments arranged by size: 2573 1156 849 580 222 213 129 72 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5794 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2664 2818 3525 Size: 416 53 83 399 24 1247 154 707 Cuts at: 3525 4483 442 Size: 958 1753 Fragments arranged by size: 1753 1247 958 707 416 399 154 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5794 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2567 4454 1239 Size: 1328 1887 2579 Fragments arranged by size: 2579 1887 1328 Bpu10I CC'TnA_GC Cuts at: 2496 2837 2496 Size: 341 5453 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3919 4784 5082 3919 Size: 865 298 4631 Fragments arranged by size: 4631 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4457 882 Size: 617 2958 2219 Fragments arranged by size: 2958 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2883 3585 493 Size: 711 1679 702 2702 Fragments arranged by size: 2702 1679 711 702 BsaBI GATnn'nnATC Cuts at: 5788 5788 Size: 5794 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2130 2228 2273 2353 Size: 53 83 186 936 597 98 45 80 Cuts at: 2353 2707 3601 3983 4618 275 Size: 354 894 382 635 1451 Fragments arranged by size: 1451 936 894 635 597 382 354 186 98 83 80 53 45 BsaWI w'CCGG_w Cuts at: 4227 4625 4899 5046 5457 4227 Size: 398 274 147 411 4564 Fragments arranged by size: 4564 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5569 Fragments arranged by size: 5569 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5794 BseYI C'CCAG_C Cuts at: 2152 2383 2959 3031 4997 5493 2152 Size: 231 576 72 1966 496 2453 Fragments arranged by size: 2453 1966 576 496 231 72 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2409 2648 1163 Size: 573 673 239 4309 Fragments arranged by size: 4309 673 573 239 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2363 2903 3244 4005 5033 1540 Size: 275 186 362 540 341 761 1028 2301 Fragments arranged by size: 2301 1028 761 540 362 341 275 186 BsiHKAI G_wGCw'C Cuts at: 2112 2568 2592 2799 2934 3006 3855 3940 5011 Size: 456 24 207 135 72 849 85 1071 Cuts at: 5011 2112 Size: 2895 Fragments arranged by size: 2895 1071 849 456 207 135 85 72 24 BsmI GAATG_Cn' Cuts at: 3305 3398 3305 Size: 93 5701 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2112 2568 2592 2799 2805 2934 Size: 399 222 100 456 24 207 6 129 Cuts at: 2934 3006 3113 3855 3940 5011 1391 Size: 72 107 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 456 399 222 207 129 107 100 85 72 24 6 BspEI T'CCGG_A Cuts at: 5457 5457 Size: 5794 BspHI T'CATG_A Cuts at: 2778 3683 5413 2778 Size: 905 1730 3159 Fragments arranged by size: 3159 1730 905 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2169 2670 3149 844 Size: 1325 501 479 3489 Fragments arranged by size: 3489 1325 501 479 BsrBI CCG'CTC Cuts at: 1394 1823 3681 1394 Size: 429 1858 3507 Fragments arranged by size: 3507 1858 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2736 4289 4463 65 Size: 1183 1488 1553 174 1396 Fragments arranged by size: 1553 1488 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2372 2898 2921 2934 2993 4438 4625 Size: 110 562 526 23 13 59 1445 187 Cuts at: 4625 1700 Size: 2869 Fragments arranged by size: 2869 1445 562 526 187 110 59 23 13 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5794 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5794 BssSI C'ACGA_G Cuts at: 2437 2798 3854 4866 2437 Size: 361 1056 1012 3365 Fragments arranged by size: 3365 1056 1012 361 BstAPI GCAn_nnn'nTGC Cuts at: 794 2610 2938 3010 794 Size: 1816 328 72 3578 Fragments arranged by size: 3578 1816 328 72 BstBI TT'CG_AA Cuts at: 4598 4598 Size: 5794 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5794 BstXI CCAn_nnnn'nTGG Cuts at: 2077 2249 2077 Size: 172 5622 BstYI r'GATC_y Cuts at: 1972 3124 3491 3876 3893 5345 5431 5443 5789 Size: 1152 367 385 17 1452 86 12 346 Cuts at: 5789 1972 Size: 1977 Fragments arranged by size: 1977 1452 1152 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2125 2125 Size: 5794 BtgI C'CryG_G Cuts at: 513 1065 2004 2055 2538 513 Size: 552 939 51 483 3769 Fragments arranged by size: 3769 939 552 483 51 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5055 Fragments arranged by size: 5055 683 56 BtsI GCAGTG_nn' Cuts at: 2331 2336 2608 3310 4104 4124 2331 Size: 5 272 702 794 20 4001 Fragments arranged by size: 4001 794 702 272 20 5 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5794 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5759 DraI TTT'AAA Cuts at: 3098 3098 Size: 5794 DraIII CAC_nnn'GTG Cuts at: 2596 2596 Size: 5794 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4801 809 Size: 507 3485 1802 Fragments arranged by size: 3485 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2052 2868 2900 Size: 54 1425 50 275 18 222 816 32 Cuts at: 2900 2919 2991 3241 4130 5759 8 Size: 19 72 250 889 1629 43 Fragments arranged by size: 1629 1425 889 816 275 250 222 72 54 50 43 32 19 18 EagI C'GGCC_G Cuts at: 1537 1812 2900 3241 1537 Size: 275 1088 341 4090 Fragments arranged by size: 4090 1088 341 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2939 3011 3724 1353 Size: 1586 72 713 3423 Fragments arranged by size: 3423 1586 713 72 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2230 2807 2885 2897 4381 4755 Size: 1064 138 641 577 78 12 1484 374 Cuts at: 4755 4901 387 Size: 146 1280 Fragments arranged by size: 1484 1280 1064 641 577 374 146 138 78 12 EcoICRI GAG'CTC Cuts at: 2110 2797 2110 Size: 687 5107 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2567 2963 3035 3857 4454 5241 5509 Size: 393 935 396 72 822 597 787 268 Cuts at: 5509 1239 Size: 1524 Fragments arranged by size: 1524 935 822 787 597 396 393 268 72 EcoO109I rG'GnC_Cy Cuts at: 2009 2009 Size: 5794 EcoRI G'AATT_C Cuts at: 3103 3481 3103 Size: 378 5416 FspI TGC'GCA Cuts at: 4300 4300 Size: 5794 HaeII r_GCGC'y Cuts at: 1678 2024 2063 2106 2177 2231 2288 2303 2424 Size: 346 39 43 71 54 57 15 121 Cuts at: 2424 2460 2645 2686 2710 4941 1678 Size: 36 185 41 24 2231 2531 Fragments arranged by size: 2531 2231 346 185 121 71 57 54 43 41 39 36 24 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4435 4467 4509 4541 1373 Size: 32 135 32 2863 32 42 32 2626 Fragments arranged by size: 2863 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3138 3384 669 Size: 988 281 1200 246 3079 Fragments arranged by size: 3079 1200 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5794 HpaI GTT'AAC Cuts at: 3384 3384 Size: 5794 KasI G'GCGC_C Cuts at: 2227 2706 2227 Size: 479 5315 KpnI G_GTAC'C Cuts at: 3119 3119 Size: 5794 MfeI C'AATT_G Cuts at: 3393 3393 Size: 5794 MluI A'CGCG_T Cuts at: 5670 5670 Size: 5794 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2094 2301 4908 5092 1305 Size: 789 207 2607 184 2007 Fragments arranged by size: 2607 2007 789 207 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3972 Fragments arranged by size: 3972 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2670 3752 4111 4270 518 Size: 648 1504 1082 359 159 2042 Fragments arranged by size: 2042 1504 1082 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2557 3010 3210 3887 5035 5280 1606 Size: 951 453 200 677 1148 245 2120 Fragments arranged by size: 2120 1148 951 677 453 245 200 NaeI GCC'GGC Cuts at: 2374 2900 2923 2995 2374 Size: 526 23 72 5173 Fragments arranged by size: 5173 526 72 23 NarI GG'CG_CC Cuts at: 2228 2707 2228 Size: 479 5315 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4303 Fragments arranged by size: 4303 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5794 NgoMIV G'CCGG_C Cuts at: 2372 2898 2921 2993 2372 Size: 526 23 72 5173 Fragments arranged by size: 5173 526 72 23 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5794 NotI GC'GGCC_GC Cuts at: 3241 3241 Size: 5794 NspI r_CATG'y Cuts at: 1132 1229 2185 2241 3152 4697 1132 Size: 97 956 56 911 1545 2229 Fragments arranged by size: 2229 1545 956 911 97 56 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5794 PciI A'CATG_T Cuts at: 4693 4693 Size: 5794 PflMI CCAn_nnn'nTGG Cuts at: 1108 2320 2539 2620 2917 1108 Size: 1212 219 81 297 3985 Fragments arranged by size: 3985 1212 297 219 81 PfoI T'CCnGG_A Cuts at: 2256 2256 Size: 5794 PmeI GTTT'AAAC Cuts at: 3098 3098 Size: 5794 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3863 3895 5376 5408 3863 Size: 32 1481 32 4249 Fragments arranged by size: 4249 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5794 PsiI TTA'TAA Cuts at: 3364 3364 Size: 5794 PspOMI G'GGCC_C Cuts at: 2008 2008 Size: 5794 PspXI vC'TCGA_Gb Cuts at: 2118 2118 Size: 5794 PstI C_TGCA'G Cuts at: 830 3084 3146 830 Size: 2254 62 3478 Fragments arranged by size: 3478 2254 62 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5794 PvuII CAG'CTG Cuts at: 3010 3010 Size: 5794 SacI G_AGCT'C Cuts at: 2112 2799 2112 Size: 687 5107 SalI G'TCGA_C Cuts at: 1936 3136 1936 Size: 1200 4594 SbfI CC_TGCA'GG Cuts at: 3146 3146 Size: 5794 ScaI AGT'ACT Cuts at: 4042 5754 4042 Size: 1712 4082 SfcI C'TryA_G Cuts at: 826 1045 2098 2449 2854 3080 3142 4277 4958 Size: 219 1053 351 405 226 62 1135 681 Cuts at: 4958 5149 826 Size: 191 1471 Fragments arranged by size: 1471 1135 1053 681 405 351 226 219 191 62 SfoI GGC'GCC Cuts at: 2229 2708 2229 Size: 479 5315 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5794 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5794 SmaI CCC'GGG Cuts at: 1872 3121 1872 Size: 1249 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2118 3898 4799 5061 820 Size: 197 926 175 1780 901 262 1553 Fragments arranged by size: 1780 1553 926 901 262 197 175 SnaBI TAC'GTA Cuts at: 493 3585 493 Size: 3092 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5794 SphI G_CATG'C Cuts at: 1132 2241 3152 1132 Size: 1109 911 3774 Fragments arranged by size: 3774 1109 911 SspI AAT'ATT Cuts at: 5 52 3718 5 Size: 47 3666 2081 Fragments arranged by size: 3666 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5794 StyI C'CwwG_G Cuts at: 513 1065 2004 3233 513 Size: 552 939 1229 3074 Fragments arranged by size: 3074 1229 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2984 3056 3821 3838 3991 4176 4621 Size: 1255 1404 72 765 17 153 185 445 Cuts at: 4621 325 Size: 1498 Fragments arranged by size: 1498 1404 1255 765 445 185 153 72 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4040 5752 96 Size: 274 80 33 51 1443 2063 1712 138 Fragments arranged by size: 2063 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5754 5786 5754 Size: 32 5762 Tth111I GACn'n_nGTC Cuts at: 1316 2221 2627 1316 Size: 905 406 4483 Fragments arranged by size: 4483 905 406 XbaI T'CTAG_A Cuts at: 3130 3130 Size: 5794 XhoI C'TCGA_G Cuts at: 1943 2118 1943 Size: 175 5619 XmaI C'CCGG_G Cuts at: 1870 3119 1870 Size: 1249 4545 XmnI GAAnn'nnTTC Cuts at: 3923 3923 Size: 5794 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3602 276 Size: 53 83 186 936 2068 2468 Fragments arranged by size: 2468 2068 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PsrI RsrII SacII SanDI SapI SexAI SfiI SrfI SwaI XcmI