################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AclI AflII AflIII AgeI Aor51HI AscI AseI AsnI AvrII BbrPI BcgI BciVI BclI BfrI BlnI BlpI Bpu1102I BsaAI BsiEI BsiWI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrGI BssHII BssSI Bst1107I BstEII BstPI BstZ17I CelII ClaI CpoI DraI EagI EclXI Eco47III Eco52I EcoO65I EcoRV EcoT22I FbaI FseI HincII HindII HpaI MfeI MluI MunI NdeI NheI NotI NruI NsiI PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI ScaI SgrAI SmaI SnaBI SpeI SspBI SspI StuI SwaI XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 55 enzymes) # ################################### AatII 1778 GACGTC GACGT^C Acc65I 2288 GGTACC G^GTACC AccI 1715 GTAGAC GT^[AC][GT]AC AccIII 2354 TCCGGA T^CCGGA Alw44I 878 GTGCAC G^TGCAC ApaI 1872 GGGCCC GGGCC^C ApaLI 878 GTGCAC G^TGCAC Asp700I 2157 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2288 GGTACC G^GTACC AspI 621 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 2414 TGCGCA TGC^GCA BamHI 186 GGATCC G^GATCC BbeI 1033 GGCGCC GGCGC^C BglII 2139 AGATCT A^GATCT BsaBI 2412 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 2354 TCCGGA T^CCGGA BsgI 1423 GTGCAG GTGCAG(16/14) BspEI 2354 TCCGGA T^CCGGA BsrDI 35 GCAATG GCAATG(2/0) BsrFI 707 GCCGGC [GA]^CCGG[CT] BstAPI 795 GCAGGGTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 2035 TTCGAA TT^CGAA Cfr10I 707 GCCGGC [GA]^CCGG[CT] DraIII 919 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 22 GACAAAAATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 347 GAATTC G^AATTC FspI 2414 TGCGCA TGC^GCA HaeII 1033 GGCGCC [GA]GCGC^[CT] HgaI 195 GACGC GACGC(5/10) HindIII 1795 AAGCTT A^AGCTT KasI 1033 GGCGCC G^GCGCC KpnI 2288 GGTACC GGTAC^C KspI 1783 CCGCGG CCGC^GG MaeII 1779 ACGT A^CGT MamI 2412 GATGCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2354 TCCGGA T^CCGGA NaeI 707 GCCGGC GCC^GGC NarI 1033 GGCGCC GG^CGCC NgoMIV 707 GCCGGC G^CCGGC NspV 2035 TTCGAA TT^CGAA PaeR7I 990 CTCGAG C^TCGAG PflFI 621 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC SacI 1990 GAGCTC GAGCT^C SacII 1783 CCGCGG CCGC^GG SapI 467 GCTCTTC GCTCTTC(1/4) SbfI 792 CCTGCAGG CCTGCA^GG SexAI 920 ACCTGGT A^CC[AT]GGT SfiI 2382 GGCCCTCCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 1033 GGCGCC GGC^GCC SfuI 2035 TTCGAA TT^CGAA Sse8387I 792 CCTGCAGG CCTGCA^GG TfiI 204 GATTC G^A[AT]TC Tth111I 621 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoI 990 CTCGAG C^TCGAG XmnI 2157 GAAGTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 347 GAATTC [GA]^AATT[CT] 1015 GAATTT AcyI 1033 GGCGCC G[GA]^CG[CT]C 1778 GACGTC AhdI 942 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1261 GACACCAAGTC ApoI 347 GAATTC [GA]^AATT[CT] 1015 GAATTT AspEI 942 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1261 GACACCAAGTC AvaI 990 CTCGAG C^[CT]CG[GA]G 1196 CTCGGG BcnI 282 CCCGG CC^[GC]GG 1965 CCGGG BglI 2296 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2383 GCCCTCCAGGC BsaHI 1033 GGCGCC G[GA]^CG[CT]C 1778 GACGTC BsoBI 990 CTCGAG C^[CT]CG[GA]G 1196 CTCGGG Eam1105I 942 GACCCTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1261 GACACCAAGTC EcoNI 1834 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2397 CCTGAGGAAGG Hin1I 1033 GGCGCC G[GA]^CG[CT]C 1778 GACGTC MseI 16 TTAA T^TAA 1646 TTAA NciI 282 CCCGG CC^[GC]GG 1965 CCGGG NcoI 2057 CCATGG C^CATGG 2150 CCATGG NspI 1341 GCATGC [GA]CATG^[CT] 1748 GCATGC PvuII 1387 CAGCTG CAG^CTG 2326 CAGCTG SphI 1341 GCATGC GCATG^C 1748 GCATGC Tru9I 16 TTAA T^TAA 1646 TTAA XcmI 914 CCAACCACCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 918 CCACCTGGTGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 102 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI AspHI AvaII BalI BanI BanII BbsI BbvCI BbvI BfaI BmyI BpmI BpuAI BsaI BsaJI BsaWI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII