(Circular) (Six-base) MAPSORT of: pFN21AE9684.seq Check: 9543 from: 1 to: 7219 pFN21AE9684 7219 bp With 182 enzymes: SgfI * December 8, 2014 13:55 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3975 3975 Size: 7219 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3787 5029 278 Size: 53 83 186 936 2251 1242 2468 Fragments arranged by size: 2468 2251 1242 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3721 4562 1937 Size: 1784 841 4594 Fragments arranged by size: 4594 1784 841 Acc65I G'GTAC_C Cuts at: 4293 4540 4293 Size: 247 6972 AclI AA'CG_TT Cuts at: 1563 5346 5719 1563 Size: 3783 373 3063 Fragments arranged by size: 3783 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3562 4262 4325 5282 6666 6934 1632 Size: 1930 700 63 957 1384 268 1917 Fragments arranged by size: 1930 1917 1384 957 700 268 63 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7022 AflIII A'CryG_T Cuts at: 1203 6118 7095 1203 Size: 4915 977 1327 Fragments arranged by size: 4915 1327 977 AgeI A'CCGG_T Cuts at: 6050 6050 Size: 7219 AhdI GACnn_n'nnGTC Cuts at: 2952 3271 5948 2952 Size: 319 2677 4223 Fragments arranged by size: 4223 2677 319 AleI CACnn'nnGTG Cuts at: 1166 2849 3422 1166 Size: 1683 573 4963 Fragments arranged by size: 4963 1683 573 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2867 1184 Size: 1683 5536 AlwNI CAG_nnn'CTG Cuts at: 2806 3335 3394 4432 4485 4962 6534 2806 Size: 529 59 1038 53 477 1572 3491 Fragments arranged by size: 3491 1572 1038 529 477 59 53 ApaI G_GGCC'C Cuts at: 3881 3881 Size: 7219 ApaLI G'TGCA_C Cuts at: 2883 5276 6432 2883 Size: 2393 1156 3670 Fragments arranged by size: 3670 2393 1156 ApoI r'AATT_y Cuts at: 1493 2352 3020 4528 4754 4906 7015 1493 Size: 859 668 1508 226 152 2109 1697 Fragments arranged by size: 2109 1697 1508 859 668 226 152 AseI AT'TA_AT Cuts at: 160 1032 5773 160 Size: 872 4741 1606 Fragments arranged by size: 4741 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7219 AvaI C'yCGr_G Cuts at: 1870 1943 2995 3201 4544 1870 Size: 73 1052 206 1343 4545 Fragments arranged by size: 4545 1343 1052 206 73 BamHI G'GATC_C Cuts at: 2191 4549 4916 2191 Size: 2358 367 4494 Fragments arranged by size: 4494 2358 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2444 2843 3038 3055 4293 Size: 325 217 627 657 399 195 17 1238 Cuts at: 4293 4540 5995 618 Size: 247 1455 1842 Fragments arranged by size: 1842 1455 1238 657 627 399 325 247 217 195 17 BanII G_rGCy'C Cuts at: 1391 2486 3209 3881 3999 4538 1391 Size: 1095 723 672 118 539 4072 Fragments arranged by size: 4072 1095 723 672 539 118 BbeI G_GCGC'C Cuts at: 3042 3042 Size: 7219 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2619 4445 4460 928 Size: 971 720 1826 15 3687 Fragments arranged by size: 3687 1826 971 720 15 BbvCI CC'TCA_GC Cuts at: 3406 3591 3977 3406 Size: 185 386 6648 Fragments arranged by size: 6648 386 185 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5410 5444 1903 Size: 34 3473 34 3678 Fragments arranged by size: 3678 3473 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5111 6321 5111 Size: 1210 6009 BclI T'GATC_A Cuts at: 1548 1548 Size: 7219 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4307 4394 5830 Size: 107 122 71 631 605 2635 87 1436 Cuts at: 5830 136 Size: 1525 Fragments arranged by size: 2635 1525 1436 631 605 122 107 87 71 BglII A'GATC_T Cuts at: 4144 7214 4144 Size: 3070 4149 BlpI GC'TnA_GC Cuts at: 4636 4636 Size: 7219 Bme1580I G_kGCm'C Cuts at: 1790 2449 2846 2887 3058 3531 3732 3881 5280 Size: 659 397 41 171 473 201 149 1399 Cuts at: 5280 6436 1790 Size: 1156 2573 Fragments arranged by size: 2573 1399 1156 659 473 397 201 171 149 41 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7219 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3083 3232 4028 Size: 416 53 83 399 24 1666 149 796 Cuts at: 4028 4950 5908 442 Size: 922 958 1753 Fragments arranged by size: 1753 1666 958 922 796 416 399 149 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7219 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3793 3793 Size: 7219 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2410 2499 3647 3899 4376 4454 5879 1239 Size: 1171 89 1148 252 477 78 1425 2579 Fragments arranged by size: 2579 1425 1171 1148 477 252 89 78 Bpu10I CC'TnA_GC Cuts at: 2538 3406 3591 3977 2538 Size: 868 185 386 5780 Fragments arranged by size: 5780 868 386 185 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2258 2452 3077 3124 3671 3685 5344 6209 6507 Size: 194 625 47 547 14 1659 865 298 Cuts at: 6507 2258 Size: 2970 Fragments arranged by size: 2970 1659 865 625 547 298 194 47 14 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2597 3305 3437 3576 3737 5882 882 Size: 617 1098 708 132 139 161 2145 2219 Fragments arranged by size: 2219 2145 1098 708 617 161 139 132 BsaAI yAC'GTr Cuts at: 493 1204 5010 493 Size: 711 3806 2702 Fragments arranged by size: 3806 2702 711 BsaBI GATnn'nnATC Cuts at: 4421 7213 4421 Size: 2792 4427 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3039 3784 5026 5408 Size: 53 83 186 936 1506 745 1242 382 Cuts at: 5408 6043 275 Size: 635 1451 Fragments arranged by size: 1506 1451 1242 936 745 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2093 3893 4359 5652 6050 6324 6471 6882 2093 Size: 1800 466 1293 398 274 147 411 2430 Fragments arranged by size: 2430 1800 1293 466 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2740 2770 2881 2911 3973 Size: 30 165 30 1337 30 111 30 1062 Cuts at: 3973 4003 4484 4514 1178 Size: 30 481 30 3883 Fragments arranged by size: 3883 1337 1062 481 165 111 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2717 2802 2983 3013 3164 3380 3584 3977 Size: 1531 85 181 30 151 216 204 393 Cuts at: 3977 3994 4007 4334 4358 4484 1186 Size: 17 13 327 24 126 3921 Fragments arranged by size: 3921 1531 393 327 216 204 181 151 126 85 30 24 17 13 BseYI C'CCAG_C Cuts at: 2404 2634 3291 3319 3350 6422 6918 2404 Size: 230 657 28 31 3072 496 2705 Fragments arranged by size: 3072 2705 657 496 230 31 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3449 1163 Size: 573 1713 4933 Fragments arranged by size: 4933 1713 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4669 5430 6458 1540 Size: 275 186 2668 761 1028 2301 Fragments arranged by size: 2668 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2734 2794 2887 2909 3829 3999 5280 5365 6436 Size: 60 93 22 920 170 1281 85 1071 Cuts at: 6436 2734 Size: 3517 Fragments arranged by size: 3517 1281 1071 920 170 93 85 60 22 BsmI GAATG_Cn' Cuts at: 2678 2714 3397 4730 4823 2678 Size: 36 683 1333 93 5074 Fragments arranged by size: 5074 1333 683 93 36 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2449 2486 2734 2794 2846 2887 2909 Size: 399 659 37 248 60 52 41 22 Cuts at: 2909 3058 3209 3531 3732 3829 3881 3999 4538 Size: 149 151 322 201 97 52 118 539 Cuts at: 4538 5280 5365 6436 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 659 539 399 322 248 201 151 149 118 97 85 60 52 52 41 37 22 BspEI T'CCGG_A Cuts at: 4359 6882 4359 Size: 2523 4696 BspHI T'CATG_A Cuts at: 5108 6838 5108 Size: 1730 5489 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2005 3918 3975 4574 844 Size: 1161 1913 57 599 3489 Fragments arranged by size: 3489 1913 1161 599 57 BsrBI CCG'CTC Cuts at: 1394 1823 3003 3168 3173 5106 1394 Size: 429 1180 165 5 1933 3507 Fragments arranged by size: 3507 1933 1180 429 165 5 BsrDI GCAATG_nn' Cuts at: 65 1248 2047 5714 5888 65 Size: 1183 799 3667 174 1396 Fragments arranged by size: 3667 1396 1183 799 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2712 5863 6050 1700 Size: 110 902 3151 187 2869 Fragments arranged by size: 3151 2869 902 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7219 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7219 BssSI C'ACGA_G Cuts at: 5279 6291 5279 Size: 1012 6207 BstAPI GCAn_nnn'nTGC Cuts at: 794 2806 794 Size: 2012 5207 BstBI TT'CG_AA Cuts at: 4041 6023 4041 Size: 1982 5237 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7219 BstXI CCAn_nnnn'nTGG Cuts at: 2149 2926 3805 2149 Size: 777 879 5563 Fragments arranged by size: 5563 879 777 BstYI r'GATC_y Cuts at: 1972 2191 4144 4482 4549 4916 5301 5318 6770 Size: 219 1953 338 67 367 385 17 1452 Cuts at: 6770 6856 6868 7214 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1953 1452 385 367 346 338 219 86 67 17 12 Bsu36I CC'TnA_GG Cuts at: 2772 2958 3704 3778 4403 2772 Size: 186 746 74 625 5588 Fragments arranged by size: 5588 746 625 186 74 BtgI C'CryG_G Cuts at: 513 1065 2547 3360 3732 3788 4000 4062 4155 Size: 552 1482 813 372 56 212 62 93 Cuts at: 4155 513 Size: 3577 Fragments arranged by size: 3577 1482 813 552 372 212 93 62 56 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6480 Fragments arranged by size: 6480 683 56 BtsI GCAGTG_nn' Cuts at: 2748 3123 3289 3523 3948 4735 5529 5549 2748 Size: 375 166 234 425 787 794 20 4418 Fragments arranged by size: 4418 794 787 425 375 234 166 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7219 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7184 DraI TTT'AAA Cuts at: 4523 4523 Size: 7219 DraIII CAC_nnn'GTG Cuts at: 2929 2929 Size: 7219 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2033 6226 809 Size: 507 717 4193 1802 Fragments arranged by size: 4193 1802 717 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2151 2226 2745 Size: 54 1425 50 275 18 321 75 519 Cuts at: 2745 2985 3735 4666 5555 7184 8 Size: 240 750 931 889 1629 43 Fragments arranged by size: 1629 1425 931 889 750 519 321 275 240 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4666 1537 Size: 275 2854 4090 Fragments arranged by size: 4090 2854 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2479 2578 4038 4184 4227 5149 1353 Size: 1126 99 1460 146 43 922 3423 Fragments arranged by size: 3423 1460 1126 922 146 99 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4391 5806 6180 6326 387 Size: 1064 138 2802 1415 374 146 1280 Fragments arranged by size: 2802 1415 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3997 3997 Size: 7219 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2410 2499 3562 3647 3899 4262 4325 Size: 393 778 89 1063 85 252 363 63 Cuts at: 4325 4376 4454 5282 5879 6666 6934 1239 Size: 51 78 828 597 787 268 1524 Fragments arranged by size: 1524 1063 828 787 778 597 393 363 268 252 89 85 78 63 51 EcoNI CCTnn'n_nnAGG Cuts at: 3843 4406 3843 Size: 563 6656 EcoO109I rG'GnC_Cy Cuts at: 3133 3181 3374 3877 3931 3133 Size: 48 193 503 54 6421 Fragments arranged by size: 6421 503 193 54 48 EcoRI G'AATT_C Cuts at: 2352 4528 4906 2352 Size: 2176 378 4665 Fragments arranged by size: 4665 2176 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3118 3694 3818 4151 3118 Size: 576 124 333 6186 Fragments arranged by size: 6186 576 333 124 FspI TGC'GCA Cuts at: 4421 5725 4421 Size: 1304 5915 FspAI rTGC'GCAy Cuts at: 4421 4421 Size: 7219 HaeII r_GCGC'y Cuts at: 1678 3042 6366 1678 Size: 1364 3324 2531 Fragments arranged by size: 3324 2531 1364 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2200 2232 2938 2970 3257 Size: 32 135 32 628 32 706 32 287 Cuts at: 3257 3289 3697 3729 4136 4168 4484 4516 5860 Size: 32 408 32 407 32 316 32 1344 Cuts at: 5860 5892 5934 5966 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1344 706 628 408 407 316 287 135 42 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4563 4809 669 Size: 988 281 2625 246 3079 Fragments arranged by size: 3079 2625 988 281 246 HindIII A'AGCT_T Cuts at: 748 3800 748 Size: 3052 4167 HpaI GTT'AAC Cuts at: 4809 4809 Size: 7219 KasI G'GCGC_C Cuts at: 3038 3038 Size: 7219 KpnI G_GTAC'C Cuts at: 4297 4544 4297 Size: 247 6972 MfeI C'AATT_G Cuts at: 4818 4818 Size: 7219 MluI A'CGCG_T Cuts at: 7095 7095 Size: 7219 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4197 6333 6517 1305 Size: 2892 2136 184 2007 Fragments arranged by size: 2892 2136 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2153 2228 2747 2987 3737 Size: 54 1425 343 321 75 519 240 750 Cuts at: 3737 10 Size: 3492 Fragments arranged by size: 3492 1425 750 519 343 321 240 75 54 MslI CAynn'nnrTG Cuts at: 518 1166 2849 3422 3839 5177 5536 5695 518 Size: 648 1683 573 417 1338 359 159 2042 Fragments arranged by size: 2042 1683 1338 648 573 417 359 159 MspA1I CmG'CkG Cuts at: 1606 3332 3394 3410 3590 3790 4333 4635 5312 Size: 1726 62 16 180 200 543 302 677 Cuts at: 5312 6460 6705 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1726 1148 677 543 302 245 200 180 62 16 NaeI GCC'GGC Cuts at: 2714 2714 Size: 7219 NarI GG'CG_CC Cuts at: 3039 3039 Size: 7219 NcoI C'CATG_G Cuts at: 513 1065 4062 4155 513 Size: 552 2997 93 3577 Fragments arranged by size: 3577 2997 552 93 NdeI CA'TA_TG Cuts at: 387 387 Size: 7219 NgoMIV G'CCGG_C Cuts at: 2712 2712 Size: 7219 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7219 NotI GC'GGCC_GC Cuts at: 4666 4666 Size: 7219 NspI r_CATG'y Cuts at: 1132 1229 3350 3757 4577 6122 1132 Size: 97 2121 407 820 1545 2229 Fragments arranged by size: 2229 2121 1545 820 407 97 PasI CC'CwG_GG Cuts at: 1348 2821 1348 Size: 1473 5746 PciI A'CATG_T Cuts at: 6118 6118 Size: 7219 PflMI CCAn_nnn'nTGG Cuts at: 1108 2411 2929 4063 4432 1108 Size: 1303 518 1134 369 3895 Fragments arranged by size: 3895 1303 1134 518 369 PfoI T'CCnGG_A Cuts at: 2515 2515 Size: 7219 PmeI GTTT'AAAC Cuts at: 4523 4523 Size: 7219 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5288 5320 6801 6833 5288 Size: 32 1481 32 5674 Fragments arranged by size: 5674 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3181 3374 3931 3181 Size: 193 557 6469 Fragments arranged by size: 6469 557 193 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7219 PsiI TTA'TAA Cuts at: 4789 4789 Size: 7219 PspOMI G'GGCC_C Cuts at: 3877 3877 Size: 7219 PspXI vC'TCGA_Gb Cuts at: 2995 2995 Size: 7219 PstI C_TGCA'G Cuts at: 830 2016 2802 2977 4332 4571 830 Size: 1186 786 175 1355 239 3478 Fragments arranged by size: 3478 1355 1186 786 239 175 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7219 PvuII CAG'CTG Cuts at: 3394 4333 3394 Size: 939 6280 SacI G_AGCT'C Cuts at: 3999 3999 Size: 7219 SacII CC_GC'GG Cuts at: 3791 3791 Size: 7219 SalI G'TCGA_C Cuts at: 1936 4561 1936 Size: 2625 4594 SapI GCTCTTCn'nnn_ Cuts at: 2479 2479 Size: 7219 SbfI CC_TGCA'GG Cuts at: 2802 4571 2802 Size: 1769 5450 ScaI AGT'ACT Cuts at: 5467 7179 5467 Size: 1712 5507 SexAI A'CCwGG_T Cuts at: 2925 2925 Size: 7219 SfcI C'TryA_G Cuts at: 826 1045 2012 2357 2798 2973 3900 4328 4567 Size: 219 967 345 441 175 927 428 239 Cuts at: 4567 5702 6383 6574 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1135 967 927 681 441 428 345 239 219 191 175 SfiI GGCCn_nnn'nGGCC Cuts at: 4394 4394 Size: 7219 SfoI GGC'GCC Cuts at: 3040 3040 Size: 7219 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7219 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7219 SmaI CCC'GGG Cuts at: 1872 4546 1872 Size: 2674 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2237 2467 2995 3092 3103 3664 Size: 197 926 294 230 528 97 11 561 Cuts at: 3664 3686 5323 6224 6486 820 Size: 22 1637 901 262 1553 Fragments arranged by size: 1637 1553 926 901 561 528 294 262 230 197 97 22 11 SnaBI TAC'GTA Cuts at: 493 5010 493 Size: 4517 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7219 SphI G_CATG'C Cuts at: 1132 3350 3757 4577 1132 Size: 2218 407 820 3774 Fragments arranged by size: 3774 2218 820 407 SspI AAT'ATT Cuts at: 5 52 5143 5 Size: 47 5091 2081 Fragments arranged by size: 5091 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7219 StyI C'CwwG_G Cuts at: 513 1065 2266 3065 3555 4062 4155 4658 513 Size: 552 1201 799 490 507 93 503 3074 Fragments arranged by size: 3074 1201 799 552 507 503 490 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2155 3548 4292 5246 5263 5416 5601 Size: 1255 575 1393 744 954 17 153 185 Cuts at: 5601 6046 325 Size: 445 1498 Fragments arranged by size: 1498 1393 1255 954 744 575 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3918 5465 7177 Size: 274 80 33 51 1443 1941 1547 1712 Cuts at: 7177 96 Size: 138 Fragments arranged by size: 1941 1712 1547 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2740 2772 2881 2913 3481 3493 3513 3525 3888 Size: 32 109 32 568 12 20 12 363 Cuts at: 3888 3920 3973 4005 7179 7211 2740 Size: 32 53 32 3174 32 2748 Fragments arranged by size: 3174 2748 568 363 109 53 32 32 32 32 32 20 12 12 Tth111I GACn'n_nGTC Cuts at: 1316 2629 1316 Size: 1313 5906 XbaI T'CTAG_A Cuts at: 4555 4555 Size: 7219 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2926 2930 2926 Size: 4 7215 XhoI C'TCGA_G Cuts at: 1943 2995 1943 Size: 1052 6167 XmaI C'CCGG_G Cuts at: 1870 4544 1870 Size: 2674 4545 XmnI GAAnn'nnTTC Cuts at: 4166 5348 4166 Size: 1182 6037 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3785 5027 276 Size: 53 83 186 936 2251 1242 2468 Fragments arranged by size: 2468 2251 1242 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoRV FseI NruI NsiI PacI PmlI PsrI RsrII SanDI SrfI SwaI