(Linear) (Six-base) MAPSORT of: KIEE9684.seq Check: 4909 from: 1 to: 2514 KIEE9684.orf With 182 enzymes: SgfI * December 5, 2014 13:30 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1970 2514 Size: 1970 544 AatII G_ACGT'C Cuts at: 0 1782 2514 Size: 1782 732 AccI GT'mk_AC Cuts at: 0 1716 2514 Size: 1716 798 Acc65I G'GTAC_C Cuts at: 0 2288 2514 Size: 2288 226 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1557 2257 2320 2514 Size: 1557 700 63 194 Fragments arranged by size: 1557 700 194 63 AhdI GACnn_n'nnGTC Cuts at: 0 947 1266 2514 Size: 947 319 1248 Fragments arranged by size: 1248 947 319 AleI CACnn'nnGTG Cuts at: 0 844 1417 2514 Size: 844 573 1097 Fragments arranged by size: 1097 844 573 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 862 2514 Size: 862 1652 AlwNI CAG_nnn'CTG Cuts at: 0 801 1330 1389 2427 2480 2514 Size: 801 529 59 1038 53 34 Fragments arranged by size: 1038 801 529 59 53 34 ApaI G_GGCC'C Cuts at: 0 1876 2514 Size: 1876 638 ApaLI G'TGCA_C Cuts at: 0 878 2514 Size: 878 1636 ApoI r'AATT_y Cuts at: 0 347 1015 2514 Size: 347 668 1499 Fragments arranged by size: 1499 668 347 AvaI C'yCGr_G Cuts at: 0 990 1196 2514 Size: 990 206 1318 Fragments arranged by size: 1318 990 206 BamHI G'GATC_C Cuts at: 0 186 2514 Size: 186 2328 BanI G'GyrC_C Cuts at: 0 439 838 1033 1050 2288 2514 Size: 439 399 195 17 1238 226 Fragments arranged by size: 1238 439 399 226 195 17 BanII G_rGCy'C Cuts at: 0 481 1204 1876 1994 2514 Size: 481 723 672 118 520 Fragments arranged by size: 723 672 520 481 118 BbeI G_GCGC'C Cuts at: 0 1037 2514 Size: 1037 1477 BbsI GAAGACnn'nnnn_ Cuts at: 0 614 2440 2455 2514 Size: 614 1826 15 59 Fragments arranged by size: 1826 614 59 15 BbvCI CC'TCA_GC Cuts at: 0 1401 1586 1972 2514 Size: 1401 185 386 542 Fragments arranged by size: 1401 542 386 185 BglI GCCn_nnn'nGGC Cuts at: 0 2302 2389 2514 Size: 2302 87 125 Fragments arranged by size: 2302 125 87 BglII A'GATC_T Cuts at: 0 2139 2514 Size: 2139 375 Bme1580I G_kGCm'C Cuts at: 0 444 841 882 1053 1526 1727 1876 2514 Size: 444 397 41 171 473 201 149 638 Fragments arranged by size: 638 473 444 397 201 171 149 41 BmrI ACTGGGnnnn_n' Cuts at: 0 1078 1227 2023 2514 Size: 1078 149 796 491 Fragments arranged by size: 1078 796 491 149 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1788 2514 Size: 1788 726 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 405 494 1642 1894 2371 2449 2514 Size: 405 89 1148 252 477 78 65 Fragments arranged by size: 1148 477 405 252 89 78 65 Bpu10I CC'TnA_GC Cuts at: 0 533 1401 1586 1972 2514 Size: 533 868 185 386 542 Fragments arranged by size: 868 542 533 386 185 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 253 447 1072 1119 1666 1680 2514 Size: 253 194 625 47 547 14 834 Fragments arranged by size: 834 625 547 253 194 47 14 BsaI GGTCTCn'nnnn_ Cuts at: 0 592 1300 1432 1571 1732 2514 Size: 592 708 132 139 161 782 Fragments arranged by size: 782 708 592 161 139 132 BsaBI GATnn'nnATC Cuts at: 0 2416 2514 Size: 2416 98 BsaHI Gr'CG_yC Cuts at: 0 1034 1779 2514 Size: 1034 745 735 Fragments arranged by size: 1034 745 735 BsaWI w'CCGG_w Cuts at: 0 88 1888 2354 2514 Size: 88 1800 466 160 Fragments arranged by size: 1800 466 160 88 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 735 765 876 906 1968 1998 2479 2509 Size: 735 30 111 30 1062 30 481 30 Cuts at: 2509 2514 Size: 5 Fragments arranged by size: 1062 735 481 111 30 30 30 30 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 712 797 978 1008 1159 1375 1579 1972 Size: 712 85 181 30 151 216 204 393 Cuts at: 1972 1989 2002 2329 2353 2479 2514 Size: 17 13 327 24 126 35 Fragments arranged by size: 712 393 327 216 204 181 151 126 85 35 30 24 17 13 BseYI C'CCAG_C Cuts at: 0 399 629 1286 1314 1345 2514 Size: 399 230 657 28 31 1169 Fragments arranged by size: 1169 657 399 230 31 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1444 2514 Size: 1444 1070 BsiHKAI G_wGCw'C Cuts at: 0 729 789 882 904 1824 1994 2514 Size: 729 60 93 22 920 170 520 Fragments arranged by size: 920 729 520 170 93 60 22 BsmI GAATG_Cn' Cuts at: 0 673 709 1392 2514 Size: 673 36 683 1122 Fragments arranged by size: 1122 683 673 36 Bsp1286I G_dGCh'C Cuts at: 0 444 481 729 789 841 882 904 1053 Size: 444 37 248 60 52 41 22 149 Cuts at: 1053 1204 1526 1727 1824 1876 1994 2514 Size: 151 322 201 97 52 118 520 Fragments arranged by size: 520 444 322 248 201 151 149 118 97 60 52 52 41 37 22 BspEI T'CCGG_A Cuts at: 0 2354 2514 Size: 2354 160 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1913 1970 2514 Size: 1913 57 544 Fragments arranged by size: 1913 544 57 BsrBI CCG'CTC Cuts at: 0 998 1163 1168 2514 Size: 998 165 5 1346 Fragments arranged by size: 1346 998 165 5 BsrDI GCAATG_nn' Cuts at: 0 42 2514 Size: 42 2472 BsrFI r'CCGG_y Cuts at: 0 707 2514 Size: 707 1807 BstAPI GCAn_nnn'nTGC Cuts at: 0 801 2514 Size: 801 1713 BstBI TT'CG_AA Cuts at: 0 2036 2514 Size: 2036 478 BstXI CCAn_nnnn'nTGG Cuts at: 0 144 921 1800 2514 Size: 144 777 879 714 Fragments arranged by size: 879 777 714 144 BstYI r'GATC_y Cuts at: 0 186 2139 2477 2514 Size: 186 1953 338 37 Fragments arranged by size: 1953 338 186 37 Bsu36I CC'TnA_GG Cuts at: 0 767 953 1699 1773 2398 2514 Size: 767 186 746 74 625 116 Fragments arranged by size: 767 746 625 186 116 74 BtgI C'CryG_G Cuts at: 0 542 1355 1727 1783 1995 2057 2150 2514 Size: 542 813 372 56 212 62 93 364 Fragments arranged by size: 813 542 372 364 212 93 62 56 BtsI GCAGTG_nn' Cuts at: 0 743 1118 1284 1518 1943 2514 Size: 743 375 166 234 425 571 Fragments arranged by size: 743 571 425 375 234 166 DraIII CAC_nnn'GTG Cuts at: 0 924 2514 Size: 924 1590 DrdI GACnn_nn'nnGTC Cuts at: 0 28 2514 Size: 28 2486 EaeI y'GGCC_r Cuts at: 0 146 221 740 980 1730 2514 Size: 146 75 519 240 750 784 Fragments arranged by size: 784 750 519 240 146 75 EarI CTCTTCn'nnn_ Cuts at: 0 474 573 2033 2179 2222 2514 Size: 474 99 1460 146 43 292 Fragments arranged by size: 1460 474 292 146 99 43 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2386 2514 Size: 2386 128 EcoICRI GAG'CTC Cuts at: 0 1992 2514 Size: 1992 522 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 405 494 1557 1642 1894 2257 2320 2371 Size: 405 89 1063 85 252 363 63 51 Cuts at: 2371 2449 2514 Size: 78 65 Fragments arranged by size: 1063 405 363 252 89 85 78 65 63 51 EcoNI CCTnn'n_nnAGG Cuts at: 0 1838 2401 2514 Size: 1838 563 113 Fragments arranged by size: 1838 563 113 EcoO109I rG'GnC_Cy Cuts at: 0 1128 1176 1369 1872 1926 2514 Size: 1128 48 193 503 54 588 Fragments arranged by size: 1128 588 503 193 54 48 EcoRI G'AATT_C Cuts at: 0 347 2514 Size: 347 2167 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 1113 1689 1813 2146 2514 Size: 1113 576 124 333 368 Fragments arranged by size: 1113 576 368 333 124 FspI TGC'GCA Cuts at: 0 2416 2514 Size: 2416 98 FspAI rTGC'GCAy Cuts at: 0 2416 2514 Size: 2416 98 HaeII r_GCGC'y Cuts at: 0 1037 2514 Size: 1037 1477 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 195 227 933 965 1252 1284 1692 1724 Size: 195 32 706 32 287 32 408 32 Cuts at: 1724 2131 2163 2479 2511 2514 Size: 407 32 316 32 3 Fragments arranged by size: 706 408 407 316 287 195 32 32 32 32 32 32 3 HindIII A'AGCT_T Cuts at: 0 1795 2514 Size: 1795 719 KasI G'GCGC_C Cuts at: 0 1033 2514 Size: 1033 1481 KpnI G_GTAC'C Cuts at: 0 2292 2514 Size: 2292 222 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 2192 2514 Size: 2192 322 MscI TGG'CCA Cuts at: 0 148 223 742 982 1732 2514 Size: 148 75 519 240 750 782 Fragments arranged by size: 782 750 519 240 148 75 MslI CAynn'nnrTG Cuts at: 0 844 1417 1834 2514 Size: 844 573 417 680 Fragments arranged by size: 844 680 573 417 MspA1I CmG'CkG Cuts at: 0 1327 1389 1405 1585 1785 2328 2514 Size: 1327 62 16 180 200 543 186 Fragments arranged by size: 1327 543 200 186 180 62 16 NaeI GCC'GGC Cuts at: 0 709 2514 Size: 709 1805 NarI GG'CG_CC Cuts at: 0 1034 2514 Size: 1034 1480 NcoI C'CATG_G Cuts at: 0 2057 2150 2514 Size: 2057 93 364 Fragments arranged by size: 2057 364 93 NgoMIV G'CCGG_C Cuts at: 0 707 2514 Size: 707 1807 NspI r_CATG'y Cuts at: 0 1345 1752 2514 Size: 1345 407 762 Fragments arranged by size: 1345 762 407 PasI CC'CwG_GG Cuts at: 0 816 2514 Size: 816 1698 PflMI CCAn_nnn'nTGG Cuts at: 0 406 924 2058 2427 2514 Size: 406 518 1134 369 87 Fragments arranged by size: 1134 518 406 369 87 PfoI T'CCnGG_A Cuts at: 0 510 2514 Size: 510 2004 PpuMI rG'GwC_Cy Cuts at: 0 1176 1369 1926 2514 Size: 1176 193 557 588 Fragments arranged by size: 1176 588 557 193 PspOMI G'GGCC_C Cuts at: 0 1872 2514 Size: 1872 642 PspXI vC'TCGA_Gb Cuts at: 0 990 2514 Size: 990 1524 PstI C_TGCA'G Cuts at: 0 11 797 972 2327 2514 Size: 11 786 175 1355 187 Fragments arranged by size: 1355 786 187 175 11 PvuII CAG'CTG Cuts at: 0 1389 2328 2514 Size: 1389 939 186 Fragments arranged by size: 1389 939 186 SacI G_AGCT'C Cuts at: 0 1994 2514 Size: 1994 520 SacII CC_GC'GG Cuts at: 0 1786 2514 Size: 1786 728 SapI GCTCTTCn'nnn_ Cuts at: 0 474 2514 Size: 474 2040 SbfI CC_TGCA'GG Cuts at: 0 797 2514 Size: 797 1717 SexAI A'CCwGG_T Cuts at: 0 920 2514 Size: 920 1594 SfcI C'TryA_G Cuts at: 0 7 352 793 968 1895 2323 2514 Size: 7 345 441 175 927 428 191 Fragments arranged by size: 927 441 428 345 191 175 7 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2389 2514 Size: 2389 125 SfoI GGC'GCC Cuts at: 0 1035 2514 Size: 1035 1479 SmlI C'TyrA_G Cuts at: 0 232 462 990 1087 1098 1659 1681 2514 Size: 232 230 528 97 11 561 22 833 Fragments arranged by size: 833 561 528 232 230 97 22 11 SphI G_CATG'C Cuts at: 0 1345 1752 2514 Size: 1345 407 762 Fragments arranged by size: 1345 762 407 StyI C'CwwG_G Cuts at: 0 261 1060 1550 2057 2150 2514 Size: 261 799 490 507 93 364 Fragments arranged by size: 799 507 490 364 261 93 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 150 1543 2287 2514 Size: 150 1393 744 227 Fragments arranged by size: 1393 744 227 150 TatI w'GTAC_w Cuts at: 0 1913 2514 Size: 1913 601 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 735 767 876 908 1476 1488 1508 1520 Size: 735 32 109 32 568 12 20 12 Cuts at: 1520 1883 1915 1968 2000 2514 Size: 363 32 53 32 514 Fragments arranged by size: 735 568 514 363 109 53 32 32 32 32 20 12 12 Tth111I GACn'n_nGTC Cuts at: 0 624 2514 Size: 624 1890 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 921 925 2514 Size: 921 4 1589 Fragments arranged by size: 1589 921 4 XhoI C'TCGA_G Cuts at: 0 990 2514 Size: 990 1524 XmnI GAAnn'nnTTC Cuts at: 0 2161 2514 Size: 2161 353 ZraI GAC'GTC Cuts at: 0 1780 2514 Size: 1780 734 Enzymes that do cut: AarI AatII AccI Acc65I AcuI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII BbeI BbsI BbvCI BglI BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BstAPI BstBI BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HindIII KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PspXI PstI PvuII SacI SacII SapI SbfI SexAI SfcI SfiI SfoI SmlI SphI StyI TaqII TatI TstI Tth111I XcmI XhoI XmnI ZraI Enzymes that do not cut: AclI AfeI AflII AflIII AgeI AloI AscI AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI BlpI BmgBI BmtI BsaAI BsiEI BsiWI BsmBI BspHI BsrGI BssHII BssSI BstEII BstZ17I BtgZI ClaI CspCI DraI EagI EcoRV FseI HincII HpaI MfeI MluI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PsrI PvuI RsrII SalI SanDI ScaI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI SwaI XbaI XmaI