################################## # NO CUT ENZYMES ( 207 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AcyI AfaI AflII AflIII AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BseAI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BsrI BssHII BssKI BssSI Bst1107I BstAPI BstBI BstEII BstNI BstPI BstYI BstZ17I Bsu36I Cac8I CelII Cfr13I ClaI CpoI DdeI DpnII DraI DraII DraIII DrdI DsaI Eam1105I Eco47III Eco81I EcoNI EcoO109I EcoO65I EcoRI EcoRII EcoRV EcoT14I EcoT22I FauI FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HinfI HpaI HphI KasI KpnI KspI MaeI MaeII MaeIII MamI MboI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MunI MvaI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau3AI Sau96I SbfI ScaI ScrFI SexAI SfaNI SfcI SfiI SfoI SfuI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tsp45I TspRI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AccII 83 CGCG CG^CG AgeI 9 ACCGGT A^CCGGT AluI 35 AGCT AG^CT AvaI 104 CTCGGG C^[CT]CG[GA]G BanI 3 GGTGCC G^G[CT][GA]CC BbvI 130 GCAGC GCAGC(8/12) BsaI 18 GGTCTC GGTCTC(1/5) BsaWI 9 ACCGGT [AT]^CCGG[AT] BsgI 13 GTGCAG GTGCAG(16/14) BsiEI 39 CGGCCG CG[GA][CT]^CG BsiYI 60 CCTCGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 60 CCTCGGTGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 19 GTCTC GTCTC(1/5) BsoBI 104 CTCGGG C^[CT]CG[GA]G BspMI 15 GCAGGT ACCTGC(4/8) BsrBI 25 CCGCTC CCGCTC(-3/-3) BsrFI 9 ACCGGT [GA]^CCGG[CT] BstF5I 153 GGATG GGATG(2/0) BstUI 83 CGCG CG^CG BstXI 173 CCAGAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CfoI 84 GCGC GCG^C Cfr10I 9 ACCGGT [GA]^CCGG[CT] EaeI 39 CGGCCG [CT]^GGCC[GA] EagI 39 CGGCCG C^GGCCG EclXI 39 CGGCCG C^GGCCG Eco52I 39 CGGCCG C^GGCCG FokI 153 GGATG GGATG(9/13) HapII 10 CCGG C^CGG HhaI 84 GCGC GCG^C HinP1I 84 GCGC G^CGC HpaII 10 CCGG C^CGG MseI 202 TTAA T^TAA MspI 10 CCGG C^CGG MvnI 83 CGCG CG^CG PinAI 9 ACCGGT A^CCGGT SgrAI 8 CACCGGTG C[GA]^CCGG[CT]G Tru9I 202 TTAA T^TAA TseI 130 GCAGC G^C[AT]GC ################################### # TWO CUT ENZYMES ( 9 enzymes) # ################################### BsaJI 60 CCTCGG C^C[ACGT][ACGT]GG 103 CCTCGG BseRI 115 GAGGAG GAGGAG(10/8) 120 GAGGAG EarI 112 GAAGAG CTCTTC(1/4) 136 GAAGAG Fnu4HI 41 GCCGC GC^[ACGT]GC 130 GCAGC HaeIII 40 GGCC GG^CC 69 GGCC ItaI 41 GCCGC GC^[ACGT]GC 130 GCAGC Ksp632I 112 GAAGAG CTCTTC(1/4) 136 GAAGAG NlaIV 3 GGTGCC GG[ACGT]^[ACGT]CC 76 GGAGCC Tsp509I 91 AATT ^AATT 179 AATT ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### AciI MboII MnlI MwoI