(Circular) (Six-base) MAPSORT of: pFN21AE4305.seq Check: 9535 from: 1 to: 5620 pFN21AE4305 5620 bp With 182 enzymes: SgfI * March 28, 2012 14:24 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2079 2774 2079 Size: 695 4925 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3430 278 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2907 2963 1937 Size: 970 56 4594 Fragments arranged by size: 4594 970 56 Acc65I G'GTAC_C Cuts at: 2122 2597 2941 2122 Size: 475 344 4801 Fragments arranged by size: 4801 475 344 AclI AA'CG_TT Cuts at: 1563 2650 3747 4120 1563 Size: 1087 1097 373 3063 Fragments arranged by size: 3063 1097 1087 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3683 5067 5335 1632 Size: 2051 1384 268 1917 Fragments arranged by size: 2051 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2155 820 Size: 197 1138 4285 Fragments arranged by size: 4285 1138 197 AflIII A'CryG_T Cuts at: 1203 4519 5496 1203 Size: 3316 977 1327 Fragments arranged by size: 3316 1327 977 AgeI A'CCGG_T Cuts at: 4451 4451 Size: 5620 AhdI GACnn_n'nnGTC Cuts at: 4349 4349 Size: 5620 AleI CACnn'nnGTG Cuts at: 1166 2157 2915 1166 Size: 991 758 3871 Fragments arranged by size: 3871 991 758 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5620 AlwNI CAG_nnn'CTG Cuts at: 3363 4935 3363 Size: 1572 4048 ApaLI G'TGCA_C Cuts at: 3677 4833 3677 Size: 1156 4464 ApoI r'AATT_y Cuts at: 1493 2525 2696 2929 3155 3307 5416 1493 Size: 1032 171 233 226 152 2109 1697 Fragments arranged by size: 2109 1697 1032 233 226 171 152 AseI AT'TA_AT Cuts at: 160 1032 4174 160 Size: 872 3142 1606 Fragments arranged by size: 3142 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 AvaI C'yCGr_G Cuts at: 1870 1943 2448 2945 1870 Size: 73 505 497 4545 Fragments arranged by size: 4545 505 497 73 BamHI G'GATC_C Cuts at: 2950 3317 2950 Size: 367 5253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2122 2597 2941 4396 618 Size: 325 217 627 335 475 344 1455 1842 Fragments arranged by size: 1842 1455 627 475 344 335 325 217 BanII G_rGCy'C Cuts at: 1391 2939 1391 Size: 1548 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2144 928 Size: 971 245 4404 Fragments arranged by size: 4404 971 245 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3811 3845 1903 Size: 34 1874 34 3678 Fragments arranged by size: 3678 1874 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2696 3512 4722 2696 Size: 816 1210 3594 Fragments arranged by size: 3594 1210 816 BclI T'GATC_A Cuts at: 1548 1548 Size: 5620 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4231 136 Size: 107 122 71 631 605 2559 1525 Fragments arranged by size: 2559 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5615 5615 Size: 5620 BlpI GC'TnA_GC Cuts at: 3037 3037 Size: 5620 Bme1580I G_kGCm'C Cuts at: 1790 3681 4837 1790 Size: 1891 1156 2573 Fragments arranged by size: 2573 1891 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5620 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3351 4309 442 Size: 416 53 83 399 24 1934 958 1753 Fragments arranged by size: 1934 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5620 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2777 2777 Size: 5620 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1996 2345 2595 2772 4280 1239 Size: 757 349 250 177 1508 2579 Fragments arranged by size: 2579 1508 757 349 250 177 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2047 2842 3745 4610 4908 2047 Size: 795 903 865 298 2759 Fragments arranged by size: 2759 903 865 795 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2736 4283 882 Size: 617 1237 1547 2219 Fragments arranged by size: 2219 1547 1237 617 BsaAI yAC'GTr Cuts at: 493 1204 3411 493 Size: 711 2207 2702 Fragments arranged by size: 2702 2207 711 BsaBI GATnn'nnATC Cuts at: 5614 5614 Size: 5620 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3427 3809 4444 275 Size: 53 83 186 936 1894 382 635 1451 Fragments arranged by size: 1894 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4053 4451 4725 4872 5283 4053 Size: 398 274 147 411 4390 Fragments arranged by size: 4390 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5395 Fragments arranged by size: 5395 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5620 BseYI C'CCAG_C Cuts at: 2264 2480 2726 4823 5319 2264 Size: 216 246 2097 496 2565 Fragments arranged by size: 2565 2097 496 246 216 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5047 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3070 3831 4859 1540 Size: 275 186 1069 761 1028 2301 Fragments arranged by size: 2301 1069 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2336 3681 3766 4837 2336 Size: 1345 85 1071 3119 Fragments arranged by size: 3119 1345 1071 85 BsmI GAATG_Cn' Cuts at: 2314 2366 3131 3224 2314 Size: 52 765 93 4710 Fragments arranged by size: 4710 765 93 52 BsmBI CGTCTCn'nnnn_ Cuts at: 2879 2879 Size: 5620 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2336 2939 3681 3766 4837 1391 Size: 399 546 603 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 603 546 399 85 BspEI T'CCGG_A Cuts at: 5283 5283 Size: 5620 BspHI T'CATG_A Cuts at: 3509 5239 3509 Size: 1730 3890 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2079 2585 2774 2975 844 Size: 1235 506 189 201 3489 Fragments arranged by size: 3489 1235 506 201 189 BsrBI CCG'CTC Cuts at: 1394 1823 3507 1394 Size: 429 1684 3507 Fragments arranged by size: 3507 1684 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2113 4115 4289 65 Size: 1183 865 2002 174 1396 Fragments arranged by size: 2002 1396 1183 865 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4264 4451 1700 Size: 110 2454 187 2869 Fragments arranged by size: 2869 2454 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5620 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5620 BssSI C'ACGA_G Cuts at: 3680 4692 3680 Size: 1012 4608 BstAPI GCAn_nnn'nTGC Cuts at: 794 2340 794 Size: 1546 4074 BstBI TT'CG_AA Cuts at: 4424 4424 Size: 5620 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5620 BstXI CCAn_nnnn'nTGG Cuts at: 2159 2198 2159 Size: 39 5581 BstYI r'GATC_y Cuts at: 1972 2221 2950 3317 3702 3719 5171 5257 5269 Size: 249 729 367 385 17 1452 86 12 Cuts at: 5269 5615 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 729 385 367 346 249 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2841 2853 513 Size: 552 1776 12 3280 Fragments arranged by size: 3280 1776 552 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2221 487 Size: 683 56 995 3886 Fragments arranged by size: 3886 995 683 56 BtsI GCAGTG_nn' Cuts at: 3136 3930 3950 3136 Size: 794 20 4806 Fragments arranged by size: 4806 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5620 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5585 DraI TTT'AAA Cuts at: 2785 2924 2785 Size: 139 5481 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4627 809 Size: 507 3311 1802 Fragments arranged by size: 3311 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2280 2856 2889 Size: 54 1425 50 275 18 450 576 33 Cuts at: 2889 3067 3956 5585 8 Size: 178 889 1629 43 Fragments arranged by size: 1629 1425 889 576 450 275 178 54 50 43 33 18 EagI C'GGCC_G Cuts at: 1537 1812 3067 1537 Size: 275 1255 4090 Fragments arranged by size: 4090 1255 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3550 1353 Size: 2197 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4207 4581 4727 387 Size: 1064 138 2618 374 146 1280 Fragments arranged by size: 2618 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 1996 2345 2595 2772 3683 4280 5067 Size: 393 364 349 250 177 911 597 787 Cuts at: 5067 5335 1239 Size: 268 1524 Fragments arranged by size: 1524 911 787 597 393 364 349 268 250 177 EcoNI CCTnn'n_nnAGG Cuts at: 2075 2075 Size: 5620 EcoO109I rG'GnC_Cy Cuts at: 2016 2016 Size: 5620 EcoRI G'AATT_C Cuts at: 2929 3307 2929 Size: 378 5242 FspI TGC'GCA Cuts at: 4126 4126 Size: 5620 HaeII r_GCGC'y Cuts at: 1678 4767 1678 Size: 3089 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2794 2826 4261 4293 4335 Size: 32 135 32 1222 32 1435 32 42 Cuts at: 4335 4367 1373 Size: 32 2626 Fragments arranged by size: 2626 1435 1222 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2964 3210 669 Size: 988 281 1026 246 3079 Fragments arranged by size: 3079 1026 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5620 HpaI GTT'AAC Cuts at: 3210 3210 Size: 5620 KpnI G_GTAC'C Cuts at: 2126 2601 2945 2126 Size: 475 344 4801 Fragments arranged by size: 4801 475 344 MfeI C'AATT_G Cuts at: 3219 3219 Size: 5620 MluI A'CGCG_T Cuts at: 5496 5496 Size: 5620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2279 2773 4734 4918 1305 Size: 974 494 1961 184 2007 Fragments arranged by size: 2007 1961 974 494 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2282 2858 10 Size: 54 1425 343 450 576 2772 Fragments arranged by size: 2772 1425 576 450 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2157 2196 2915 3578 3937 4096 518 Size: 648 991 39 719 663 359 159 2042 Fragments arranged by size: 2042 991 719 663 648 359 159 39 MspA1I CmG'CkG Cuts at: 1606 2843 3036 3713 4861 5106 1606 Size: 1237 193 677 1148 245 2120 Fragments arranged by size: 2120 1237 1148 677 245 193 NcoI C'CATG_G Cuts at: 513 1065 2853 513 Size: 552 1788 3280 Fragments arranged by size: 3280 1788 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5620 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5620 NotI GC'GGCC_GC Cuts at: 3067 3067 Size: 5620 NspI r_CATG'y Cuts at: 1132 1229 2978 4523 1132 Size: 97 1749 1545 2229 Fragments arranged by size: 2229 1749 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5620 PciI A'CATG_T Cuts at: 4519 4519 Size: 5620 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5620 PfoI T'CCnGG_A Cuts at: 2012 2012 Size: 5620 PmeI GTTT'AAAC Cuts at: 2924 2924 Size: 5620 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3689 3721 5202 5234 3689 Size: 32 1481 32 4075 Fragments arranged by size: 4075 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2016 2016 Size: 5620 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5620 PsiI TTA'TAA Cuts at: 3190 3190 Size: 5620 PspXI vC'TCGA_Gb Cuts at: 2448 2448 Size: 5620 PstI C_TGCA'G Cuts at: 830 2076 2470 2972 830 Size: 1246 394 502 3478 Fragments arranged by size: 3478 1246 502 394 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5620 SacII CC_GC'GG Cuts at: 2844 2844 Size: 5620 SalI G'TCGA_C Cuts at: 1936 2962 1936 Size: 1026 4594 SbfI CC_TGCA'GG Cuts at: 2972 2972 Size: 5620 ScaI AGT'ACT Cuts at: 3868 5580 3868 Size: 1712 3908 SfcI C'TryA_G Cuts at: 826 1045 2072 2466 2904 2968 4103 4784 4975 Size: 219 1027 394 438 64 1135 681 191 Cuts at: 4975 826 Size: 1471 Fragments arranged by size: 1471 1135 1027 681 438 394 219 191 64 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5620 SmaI CCC'GGG Cuts at: 1872 2947 1872 Size: 1075 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2062 2155 2448 2821 3724 4625 Size: 197 926 119 93 293 373 903 901 Cuts at: 4625 4887 820 Size: 262 1553 Fragments arranged by size: 1553 926 903 901 373 293 262 197 119 93 SnaBI TAC'GTA Cuts at: 493 3411 493 Size: 2918 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5620 SphI G_CATG'C Cuts at: 1132 2978 1132 Size: 1846 3774 SspI AAT'ATT Cuts at: 5 52 3544 5 Size: 47 3492 2081 Fragments arranged by size: 3492 2081 47 StuI AGG'CCT Cuts at: 1517 2349 1517 Size: 832 4788 StyI C'CwwG_G Cuts at: 513 1065 2506 2853 3059 513 Size: 552 1441 347 206 3074 Fragments arranged by size: 3074 1441 552 347 206 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3647 3664 3817 4002 4447 325 Size: 1255 2067 17 153 185 445 1498 Fragments arranged by size: 2067 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3866 5578 96 Size: 274 80 33 51 1443 1889 1712 138 Fragments arranged by size: 1889 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5580 5612 5580 Size: 32 5588 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5620 XbaI T'CTAG_A Cuts at: 2956 2956 Size: 5620 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2696 2696 Size: 5620 XhoI C'TCGA_G Cuts at: 1943 2448 1943 Size: 505 5115 XmaI C'CCGG_G Cuts at: 1870 2945 1870 Size: 1075 4545 XmnI GAAnn'nnTTC Cuts at: 3749 3749 Size: 5620 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3428 276 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI PvuII RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI