################################## # NO CUT ENZYMES ( 87 enzymes) # ################################## AatII Acc65I AccII AclI AflIII ApaI AscI AseI AsnI Asp718I AspI AviII AvrII BbrPI BbvCI BcgI BclI BglI BlnI BsaAI BsaBI BsaI BsiWI Bsp1407I BspDI BspLU11I BsrGI BssHII BssSI BstAPI BstEII BstPI BstUI ClaI CpoI DraI DraIII EagI EclXI Eco52I EcoNI EcoO65I FbaI FseI FspI HpaI KpnI KspI MamI MfeI MluI MunI MvnI NaeI NdeI NgoMIV NheI NotI NruI PacI PflFI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Tth111I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccIII 1708 TCCGGA T^CCGGA AflII 411 CTTAAG C^TTAAG AgeI 1343 ACCGGT A^CCGGT AhdI 2659 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 178 GTGCAC G^TGCAC AocI 2007 CCTGAGG CC^T[ACGT]AGG Aor51HI 2397 AGCGCT AGC^GCT ApaLI 178 GTGCAC G^TGCAC Asp700I 1357 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 2659 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1667 GGATCC G^GATCC BbeI 960 GGCGCC GGCGC^C BciVI 1530 GTATCC GTATCC(6/5) BfrI 411 CTTAAG C^TTAAG BglII 500 AGATCT A^GATCT BlpI 2242 GCTGAGC GC^T[ACGT]AGC Bpu1102I 2242 GCTGAGC GC^T[ACGT]AGC BseAI 1708 TCCGGA T^CCGGA BsiEI 947 CGGTCG CG[GA][CT]^CG BsmI 652 GCATTC GAATGC(1/-1) BspEI 1708 TCCGGA T^CCGGA BspHI 1595 TCATGA T^CATGA BsrDI 1224 CATTGC GCAATG(2/0) BsrFI 1343 ACCGGT [GA]^CCGG[CT] Bst1107I 1337 GTATAC GTA^TAC BstBI 1364 TTCGAA TT^CGAA BstZ17I 1337 GTATAC GTA^TAC Bsu36I 2007 CCTGAGG CC^T[ACGT]AGG CelII 2242 GCTGAGC GC^T[ACGT]AGC Cfr10I 1343 ACCGGT [GA]^CCGG[CT] Eam1105I 2659 GACCTGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 2397 AGCGCT AGC^GCT Eco81I 2007 CCTGAGG CC^T[ACGT]AGG EcoRI 2338 GAATTC G^AATTC EcoT22I 1213 ATGCAT ATGCA^T HincII 204 GTTGAC GT[CT]^[GA]AC HindII 204 GTTGAC GT[CT]^[GA]AC HindIII 1423 AAGCTT A^AGCTT KasI 960 GGCGCC G^GCGCC MroI 1708 TCCGGA T^CCGGA NarI 960 GGCGCC GG^CGCC NsiI 1213 ATGCAT ATGCA^T NspI 1389 GCATGC [GA]CATG^[CT] NspV 1364 TTCGAA TT^CGAA PflMI 1079 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 1343 ACCGGT A^CCGGT RcaI 1595 TCATGA T^CATGA SapI 1378 GAAGAGC GCTCTTC(1/4) SfoI 960 GGCGCC GGC^GCC SfuI 1364 TTCGAA TT^CGAA SphI 1389 GCATGC GCATG^C Van91I 1079 CCATTATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 1673 CCACCACAGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 1357 GAACTGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AccI 769 GTAGAC GT^[AC][GT]AC 1337 GTATAC AcyI 960 GGCGCC G[GA]^CG[CT]C 2673 GACGCC AvaI 127 CTCGAG C^[CT]CG[GA]G 2079 CTCGAG BalI 2 TGGCCA TGG^CCA 575 TGGCCA BcnI 561 CCGGG CC^[GC]GG 2074 CCCGG BpmI 2393 CTCCAG CTGGAG(16/14) 2121 CTGGAG BsaHI 960 GGCGCC G[GA]^CG[CT]C 2673 GACGCC BsaWI 1343 ACCGGT [AT]^CCGG[AT] 1708 TCCGGA BsgI 1861 CTGCAC GTGCAG(16/14) 2202 GTGCAG BsmBI 280 GAGACG CGTCTC(1/5) 604 GAGACG BsoBI 127 CTCGAG C^[CT]CG[GA]G 2079 CTCGAG DrdI 282 GACGGCGGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 2751 GACTCAGATGTC EcoRV 610 GATATC GAT^ATC 1748 GATATC FauI 1431 GCGGG CCCGC(4/6) 1485 GCGGG HaeII 960 GGCGCC [GA]GCGC^[CT] 2397 AGCGCT HgaI 2179 GACGC GACGC(5/10) 2673 GACGC Hin1I 960 GGCGCC G[GA]^CG[CT]C 2673 GACGCC MaeII 1165 ACGT A^CGT 2787 ACGT MluNI 2 TGGCCA TGG^CCA 575 TGGCCA MscI 2 TGGCCA TGG^CCA 575 TGGCCA MseI 412 TTAA T^TAA 1502 TTAA NciI 561 CCGGG CC^[GC]GG 2074 CCCGG PaeR7I 127 CTCGAG C^TCGAG 2079 CTCGAG PpuMI 1537 GGGACCT [GA]G^G[AT]CC[CT] 2221 GGGTCCT PvuII 2068 CAGCTG CAG^CTG 2520 CAGCTG StuI 415 AGGCCT AGG^CCT 2416 AGGCCT Tru9I 412 TTAA T^TAA 1502 TTAA XhoI 127 CTCGAG C^TCGAG 2079 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BanI BanII BbsI BbvI BfaI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NcoI NdeII NlaIII NlaIV PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII