(Linear) (Six-base) MAPSORT of: KIBB0460.seq Check: 1845 from: 1 to: 2898 KIBB0460.orf With 182 enzymes: SgfI * March 22, 2012 15:30 .. AccI GT'mk_AC Cuts at: 0 770 1338 2898 Size: 770 568 1560 Fragments arranged by size: 1560 770 568 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 238 359 1058 2182 2819 2898 Size: 36 202 121 699 1124 637 79 Fragments arranged by size: 1124 699 637 202 121 79 36 AfeI AGC'GCT Cuts at: 0 2399 2898 Size: 2399 499 AflII C'TTAA_G Cuts at: 0 411 2898 Size: 411 2487 AgeI A'CCGG_T Cuts at: 0 1343 2898 Size: 1343 1555 AhdI GACnn_n'nnGTC Cuts at: 0 2664 2898 Size: 2664 234 AleI CACnn'nnGTG Cuts at: 0 199 1681 2898 Size: 199 1482 1217 Fragments arranged by size: 1482 1217 199 AlwNI CAG_nnn'CTG Cuts at: 0 519 2348 2522 2898 Size: 519 1829 174 376 Fragments arranged by size: 1829 519 376 174 ApaLI G'TGCA_C Cuts at: 0 178 2898 Size: 178 2720 ApoI r'AATT_y Cuts at: 0 881 1839 2338 2482 2553 2898 Size: 881 958 499 144 71 345 Fragments arranged by size: 958 881 499 345 144 71 AvaI C'yCGr_G Cuts at: 0 127 2079 2898 Size: 127 1952 819 Fragments arranged by size: 1952 819 127 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 906 939 2898 Size: 906 33 1959 Fragments arranged by size: 1959 906 33 BamHI G'GATC_C Cuts at: 0 1667 2898 Size: 1667 1231 BanI G'GyrC_C Cuts at: 0 960 2371 2386 2898 Size: 960 1411 15 512 Fragments arranged by size: 1411 960 512 15 BanII G_rGCy'C Cuts at: 0 92 534 1385 1499 2044 2086 2263 2717 Size: 92 442 851 114 545 42 177 454 Cuts at: 2717 2898 Size: 181 Fragments arranged by size: 851 545 454 442 181 177 114 92 42 BbeI G_GCGC'C Cuts at: 0 964 2898 Size: 964 1934 BbsI GAAGACnn'nnnn_ Cuts at: 0 347 537 2499 2639 2755 2898 Size: 347 190 1962 140 116 143 Fragments arranged by size: 1962 347 190 143 140 116 BciVI GTATCCnnnnn_n' Cuts at: 0 1541 2898 Size: 1541 1357 BfrBI ATG'CAT Cuts at: 0 1215 2898 Size: 1215 1683 BglII A'GATC_T Cuts at: 0 500 2898 Size: 500 2398 BlpI GC'TnA_GC Cuts at: 0 2243 2898 Size: 2243 655 Bme1580I G_kGCm'C Cuts at: 0 182 711 2898 Size: 182 529 2187 Fragments arranged by size: 2187 529 182 BmgBI CAC'GTC Cuts at: 0 1166 2898 Size: 1166 1732 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 519 1196 1399 2574 2898 Size: 519 677 203 1175 324 Fragments arranged by size: 1175 677 519 324 203 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2142 2378 2898 Size: 2142 236 520 Fragments arranged by size: 2142 520 236 Bpu10I CC'TnA_GC Cuts at: 0 22 259 1384 2898 Size: 22 237 1125 1514 Fragments arranged by size: 1514 1125 237 22 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1447 2898 Size: 1447 1451 BsaHI Gr'CG_yC Cuts at: 0 961 2674 2898 Size: 961 1713 224 Fragments arranged by size: 1713 961 224 BsaWI w'CCGG_w Cuts at: 0 1343 1708 2898 Size: 1343 365 1190 Fragments arranged by size: 1343 1190 365 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 2080 2110 2898 Size: 2080 30 788 Fragments arranged by size: 2080 788 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 21 511 2271 2433 2898 Size: 21 490 1760 162 465 Fragments arranged by size: 1760 490 465 162 21 BseYI C'CCAG_C Cuts at: 0 519 2022 2898 Size: 519 1503 876 Fragments arranged by size: 1503 876 519 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1846 2223 2898 Size: 1846 377 675 Fragments arranged by size: 1846 675 377 BsiEI CG_ry'CG Cuts at: 0 950 2898 Size: 950 1948 BsiHKAI G_wGCw'C Cuts at: 0 20 92 182 328 1385 1499 2898 Size: 20 72 90 146 1057 114 1399 Fragments arranged by size: 1399 1057 146 114 90 72 20 BsmI GAATG_Cn' Cuts at: 0 652 2898 Size: 652 2246 BsmBI CGTCTCn'nnnn_ Cuts at: 0 274 598 2898 Size: 274 324 2300 Fragments arranged by size: 2300 324 274 Bsp1286I G_dGCh'C Cuts at: 0 20 92 182 328 534 711 1385 1499 Size: 20 72 90 146 206 177 674 114 Cuts at: 1499 2044 2086 2263 2717 2898 Size: 545 42 177 454 181 Fragments arranged by size: 674 545 454 206 181 177 177 146 114 90 72 42 20 BspEI T'CCGG_A Cuts at: 0 1708 2898 Size: 1708 1190 BspHI T'CATG_A Cuts at: 0 1595 2898 Size: 1595 1303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 353 1868 2002 2669 2898 Size: 353 1515 134 667 229 Fragments arranged by size: 1515 667 353 229 134 BsrBI CCG'CTC Cuts at: 0 541 1419 1431 2898 Size: 541 878 12 1467 Fragments arranged by size: 1467 878 541 12 BsrDI GCAATG_nn' Cuts at: 0 1223 2898 Size: 1223 1675 BsrFI r'CCGG_y Cuts at: 0 1343 2898 Size: 1343 1555 BstBI TT'CG_AA Cuts at: 0 1365 2898 Size: 1365 1533 BstXI CCAn_nnnn'nTGG Cuts at: 0 1584 1683 2106 2898 Size: 1584 99 423 792 Fragments arranged by size: 1584 792 423 99 BstYI r'GATC_y Cuts at: 0 500 1049 1667 2898 Size: 500 549 618 1231 Fragments arranged by size: 1231 618 549 500 BstZ17I GTA'TAC Cuts at: 0 1339 2898 Size: 1339 1559 Bsu36I CC'TnA_GG Cuts at: 0 2008 2898 Size: 2008 890 BtgI C'CryG_G Cuts at: 0 711 1583 2105 2898 Size: 711 872 522 793 Fragments arranged by size: 872 793 711 522 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 633 2491 2898 Size: 633 1858 407 Fragments arranged by size: 1858 633 407 BtsI GCAGTG_nn' Cuts at: 0 378 384 520 1523 1691 2898 Size: 378 6 136 1003 168 1207 Fragments arranged by size: 1207 1003 378 168 136 6 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 768 803 2898 Size: 768 35 2095 Fragments arranged by size: 2095 768 35 DrdI GACnn_nn'nnGTC Cuts at: 0 288 2757 2898 Size: 288 2469 141 Fragments arranged by size: 2469 288 141 EaeI y'GGCC_r Cuts at: 0 2 575 1642 2898 Size: 2 573 1067 1256 Fragments arranged by size: 1256 1067 573 2 EarI CTCTTCn'nnn_ Cuts at: 0 39 563 1373 1895 2422 2444 2570 2838 Size: 39 524 810 522 527 22 126 268 Cuts at: 2838 2898 Size: 60 Fragments arranged by size: 810 527 524 522 268 126 60 39 22 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 580 2898 Size: 580 2318 EcoICRI GAG'CTC Cuts at: 0 90 1383 1497 2898 Size: 90 1293 114 1401 Fragments arranged by size: 1401 1293 114 90 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 36 238 359 1058 2142 2182 2378 2819 Size: 36 202 121 699 1084 40 196 441 Cuts at: 2819 2898 Size: 79 Fragments arranged by size: 1084 699 441 202 196 121 79 40 36 EcoO109I rG'GnC_Cy Cuts at: 0 675 1538 2222 2501 2898 Size: 675 863 684 279 397 Fragments arranged by size: 863 684 675 397 279 EcoRI G'AATT_C Cuts at: 0 2338 2898 Size: 2338 560 EcoRV GAT'ATC Cuts at: 0 612 1750 2898 Size: 612 1138 1148 Fragments arranged by size: 1148 1138 612 HaeII r_GCGC'y Cuts at: 0 964 2401 2898 Size: 964 1437 497 Fragments arranged by size: 1437 964 497 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 741 773 826 858 994 1026 1133 1165 Size: 741 32 53 32 136 32 107 32 Cuts at: 1165 1213 1245 1734 1766 1941 1973 2080 2112 Size: 48 32 489 32 175 32 107 32 Cuts at: 2112 2221 2253 2428 2460 2490 2522 2650 2682 Size: 109 32 175 32 30 32 128 32 Cuts at: 2682 2683 2701 2715 2733 2815 2847 2898 Size: 1 18 14 18 82 32 51 Fragments arranged by size: 741 489 175 175 136 128 109 107 107 82 53 51 48 32 32 32 32 32 32 32 32 32 32 32 32 32 30 18 18 14 1 HincII GTy'rAC Cuts at: 0 206 2898 Size: 206 2692 HindIII A'AGCT_T Cuts at: 0 1423 2898 Size: 1423 1475 KasI G'GCGC_C Cuts at: 0 960 2898 Size: 960 1938 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 1074 2898 Size: 1074 1824 MscI TGG'CCA Cuts at: 0 4 577 2898 Size: 4 573 2321 Fragments arranged by size: 2321 573 4 MslI CAynn'nnrTG Cuts at: 0 97 199 869 1577 1681 2104 2708 2898 Size: 97 102 670 708 104 423 604 190 Fragments arranged by size: 708 670 604 423 190 104 102 97 MspA1I CmG'CkG Cuts at: 0 2070 2345 2522 2898 Size: 2070 275 177 376 Fragments arranged by size: 2070 376 275 177 NarI GG'CG_CC Cuts at: 0 961 2898 Size: 961 1937 NcoI C'CATG_G Cuts at: 0 711 1583 2105 2898 Size: 711 872 522 793 Fragments arranged by size: 872 793 711 522 NsiI A_TGCA'T Cuts at: 0 1217 2898 Size: 1217 1681 NspI r_CATG'y Cuts at: 0 1393 2898 Size: 1393 1505 PasI CC'CwG_GG Cuts at: 0 2159 2898 Size: 2159 739 PflMI CCAn_nnn'nTGG Cuts at: 0 1085 2898 Size: 1085 1813 PpuMI rG'GwC_Cy Cuts at: 0 1538 2222 2898 Size: 1538 684 676 Fragments arranged by size: 1538 684 676 PspXI vC'TCGA_Gb Cuts at: 0 127 2079 2898 Size: 127 1952 819 Fragments arranged by size: 1952 819 127 PstI C_TGCA'G Cuts at: 0 515 1236 1475 1913 2115 2426 2898 Size: 515 721 239 438 202 311 472 Fragments arranged by size: 721 515 472 438 311 239 202 PvuII CAG'CTG Cuts at: 0 2070 2522 2898 Size: 2070 452 376 Fragments arranged by size: 2070 452 376 SacI G_AGCT'C Cuts at: 0 92 1385 1499 2898 Size: 92 1293 114 1399 Fragments arranged by size: 1399 1293 114 92 SapI GCTCTTCn'nnn_ Cuts at: 0 1373 2898 Size: 1373 1525 SfcI C'TryA_G Cuts at: 0 511 1232 1471 1909 2111 2349 2422 2880 Size: 511 721 239 438 202 238 73 458 Cuts at: 2880 2898 Size: 18 Fragments arranged by size: 721 511 458 438 239 238 202 73 18 SfoI GGC'GCC Cuts at: 0 962 2898 Size: 962 1936 SmlI C'TyrA_G Cuts at: 0 127 411 1426 2079 2898 Size: 127 284 1015 653 819 Fragments arranged by size: 1015 819 653 284 127 SphI G_CATG'C Cuts at: 0 1393 2898 Size: 1393 1505 StuI AGG'CCT Cuts at: 0 417 2418 2898 Size: 417 2001 480 Fragments arranged by size: 2001 480 417 StyI C'CwwG_G Cuts at: 0 534 711 1583 2105 2898 Size: 534 177 872 522 793 Fragments arranged by size: 872 793 534 522 177 TatI w'GTAC_w Cuts at: 0 667 1239 2898 Size: 667 572 1659 Fragments arranged by size: 1659 667 572 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1680 2898 Size: 1680 1218 XhoI C'TCGA_G Cuts at: 0 127 2079 2898 Size: 127 1952 819 Fragments arranged by size: 1952 819 127 XmnI GAAnn'nnTTC Cuts at: 0 1361 2898 Size: 1361 1537 Enzymes that do cut: AccI AcuI AfeI AflII AgeI AhdI AleI AlwNI ApaLI ApoI AvaI BaeI BamHI BanI BanII BbeI BbsI BciVI BfrBI BglII BlpI Bme1580I BmgBI BplI BpmI Bpu10I BpuEI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BstBI BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI CspCI DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV HaeII Hin4I HincII HindIII KasI MmeI MscI MslI MspA1I NarI NcoI NsiI NspI PasI PflMI PpuMI PspXI PstI PvuII SacI SapI SfcI SfoI SmlI SphI StuI StyI TatI XcmI XhoI XmnI Enzymes that do not cut: AarI AatII Acc65I AclI AflIII AlfI AloI ApaI AscI AseI AsiSI AvrII BbvCI BcgI BclI BglI BmrI BmtI BsaI BsaAI BsaBI BsiWI BsrGI BssHII BssSI BstAPI BstEII ClaI DraI DraIII EagI EcoNI FalI FseI FspI FspAI HpaI KpnI MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PvuI RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TaqII TstI Tth111I XbaI XmaI ZraI