(Circular) (Six-base) MAPSORT of: pFN21AE3142.seq Check: 1880 from: 1 to: 5764 pFN21AE3142 5764 bp With 182 enzymes: SgfI * March 28, 2012 12:51 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2475 2475 Size: 5764 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3574 278 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2915 3107 1937 Size: 978 192 4594 Fragments arranged by size: 4594 978 192 Acc65I G'GTAC_C Cuts at: 3085 3085 Size: 5764 AclI AA'CG_TT Cuts at: 1563 3891 4264 1563 Size: 2328 373 3063 Fragments arranged by size: 3063 2328 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2681 3827 5211 5479 1632 Size: 1049 1146 1384 268 1917 Fragments arranged by size: 1917 1384 1146 1049 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5567 AflIII A'CryG_T Cuts at: 1203 4663 5640 1203 Size: 3460 977 1327 Fragments arranged by size: 3460 1327 977 AgeI A'CCGG_T Cuts at: 4595 4595 Size: 5764 AhdI GACnn_n'nnGTC Cuts at: 4493 4493 Size: 5764 AleI CACnn'nnGTG Cuts at: 1166 2951 1166 Size: 1785 3979 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5764 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2386 2418 2696 2728 2386 Size: 32 278 32 5422 Fragments arranged by size: 5422 278 32 32 AlwNI CAG_nnn'CTG Cuts at: 3049 3507 5079 3049 Size: 458 1572 3734 Fragments arranged by size: 3734 1572 458 ApaI G_GGCC'C Cuts at: 2377 2533 2377 Size: 156 5608 ApaLI G'TGCA_C Cuts at: 3821 4977 3821 Size: 1156 4608 ApoI r'AATT_y Cuts at: 1493 3073 3299 3451 5560 1493 Size: 1580 226 152 2109 1697 Fragments arranged by size: 2109 1697 1580 226 152 AseI AT'TA_AT Cuts at: 160 1032 4318 160 Size: 872 3286 1606 Fragments arranged by size: 3286 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 AvaI C'yCGr_G Cuts at: 1870 1943 3089 1870 Size: 73 1146 4545 Fragments arranged by size: 4545 1146 73 AvrII C'CTAG_G Cuts at: 2809 2809 Size: 5764 BamHI G'GATC_C Cuts at: 2434 3094 3461 2434 Size: 660 367 4737 Fragments arranged by size: 4737 660 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3085 4540 618 Size: 325 217 627 1298 1455 1842 Fragments arranged by size: 1842 1455 1298 627 325 217 BanII G_rGCy'C Cuts at: 1391 2377 2533 2589 2818 3083 1391 Size: 986 156 56 229 265 4072 Fragments arranged by size: 4072 986 265 229 156 56 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4793 BbvCI CC'TCA_GC Cuts at: 2632 2632 Size: 5764 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3955 3989 1903 Size: 34 2018 34 3678 Fragments arranged by size: 3678 2018 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2732 2745 3656 4866 2732 Size: 13 911 1210 3630 Fragments arranged by size: 3630 1210 911 13 BclI T'GATC_A Cuts at: 1548 1548 Size: 5764 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4375 136 Size: 107 122 71 631 605 2703 1525 Fragments arranged by size: 2703 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5759 5759 Size: 5764 BlpI GC'TnA_GC Cuts at: 3181 3181 Size: 5764 Bme1580I G_kGCm'C Cuts at: 1790 2156 2377 2533 3825 4981 1790 Size: 366 221 156 1292 1156 2573 Fragments arranged by size: 2573 1292 1156 366 221 156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5764 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2318 3495 4453 Size: 416 53 83 399 24 901 1177 958 Cuts at: 4453 442 Size: 1753 Fragments arranged by size: 1753 1177 958 901 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5764 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2300 2698 2852 4424 1239 Size: 1061 398 154 1572 2579 Fragments arranged by size: 2579 1572 1061 398 154 Bpu10I CC'TnA_GC Cuts at: 2453 2632 2453 Size: 179 5585 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3889 4754 5052 3889 Size: 865 298 4601 Fragments arranged by size: 4601 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2231 4427 882 Size: 617 732 2196 2219 Fragments arranged by size: 2219 2196 732 617 BsaAI yAC'GTr Cuts at: 493 1204 3555 493 Size: 711 2351 2702 Fragments arranged by size: 2702 2351 711 BsaBI GATnn'nnATC Cuts at: 5758 5758 Size: 5764 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3571 3953 4588 275 Size: 53 83 186 936 2038 382 635 1451 Fragments arranged by size: 2038 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4197 4595 4869 5016 5427 4197 Size: 398 274 147 411 4534 Fragments arranged by size: 4534 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2454 2484 2696 2726 2834 Size: 30 165 30 1051 30 212 30 108 Cuts at: 2834 2850 2864 2880 2884 2914 3023 3053 1178 Size: 16 14 16 4 30 109 30 3889 Fragments arranged by size: 3889 1051 212 165 109 108 30 30 30 30 30 30 16 16 14 4 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2015 2067 2249 2354 1186 Size: 829 52 182 105 4596 Fragments arranged by size: 4596 829 182 105 52 BseYI C'CCAG_C Cuts at: 2397 2552 4967 5463 2397 Size: 155 2415 496 2698 Fragments arranged by size: 2698 2415 496 155 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5191 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3214 3975 5003 1540 Size: 275 186 1213 761 1028 2301 Fragments arranged by size: 2301 1213 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2589 3825 3910 4981 2589 Size: 1236 85 1071 3372 Fragments arranged by size: 3372 1236 1071 85 BsmI GAATG_Cn' Cuts at: 3275 3368 3275 Size: 93 5671 BsmBI CGTCTCn'nnnn_ Cuts at: 2366 2366 Size: 5764 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2156 2377 2533 2589 2818 3083 3825 Size: 399 366 221 156 56 229 265 742 Cuts at: 3825 3910 4981 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 366 265 229 221 156 85 56 BspEI T'CCGG_A Cuts at: 5427 5427 Size: 5764 BspHI T'CATG_A Cuts at: 3653 5383 3653 Size: 1730 4034 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2475 3119 844 Size: 1631 644 3489 Fragments arranged by size: 3489 1631 644 BsrBI CCG'CTC Cuts at: 1394 1823 3651 1394 Size: 429 1828 3507 Fragments arranged by size: 3507 1828 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4259 4433 65 Size: 1183 3011 174 1396 Fragments arranged by size: 3011 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4408 4595 1700 Size: 110 2598 187 2869 Fragments arranged by size: 2869 2598 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5764 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5764 BssSI C'ACGA_G Cuts at: 2027 2775 3824 4836 2027 Size: 748 1049 1012 2955 Fragments arranged by size: 2955 1049 1012 748 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5764 BstBI TT'CG_AA Cuts at: 4568 4568 Size: 5764 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5764 BstXI CCAn_nnnn'nTGG Cuts at: 2722 2953 2722 Size: 231 5533 BstYI r'GATC_y Cuts at: 1972 2434 3094 3461 3846 3863 5315 5401 5413 Size: 462 660 367 385 17 1452 86 12 Cuts at: 5413 5759 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 660 462 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2916 2916 Size: 5764 Bsu36I CC'TnA_GG Cuts at: 2830 2830 Size: 5764 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4273 Fragments arranged by size: 4273 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5025 Fragments arranged by size: 5025 683 56 BtsI GCAGTG_nn' Cuts at: 2271 2625 3280 4074 4094 2271 Size: 354 655 794 20 3941 Fragments arranged by size: 3941 794 655 354 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5764 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2922 2957 529 Size: 35 2358 35 3336 Fragments arranged by size: 3336 2358 35 35 DraI TTT'AAA Cuts at: 3068 3068 Size: 5764 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2096 2126 2358 4771 809 Size: 507 780 30 232 2413 1802 Fragments arranged by size: 2413 1802 780 507 232 30 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3211 4100 5729 Size: 54 1425 50 275 18 1381 889 1629 Cuts at: 5729 8 Size: 43 Fragments arranged by size: 1629 1425 1381 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3211 1537 Size: 275 1399 4090 Fragments arranged by size: 4090 1399 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2927 2934 3021 3694 1353 Size: 1574 7 87 673 3423 Fragments arranged by size: 3423 1574 673 87 7 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4351 4725 4871 387 Size: 1064 138 2762 374 146 1280 Fragments arranged by size: 2762 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2587 2587 Size: 5764 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2300 2681 2698 2852 3827 4424 5211 Size: 393 668 381 17 154 975 597 787 Cuts at: 5211 5479 1239 Size: 268 1524 Fragments arranged by size: 1524 975 787 668 597 393 381 268 154 17 EcoNI CCTnn'n_nnAGG Cuts at: 2800 2800 Size: 5764 EcoO109I rG'GnC_Cy Cuts at: 2312 2373 2374 2530 2718 2805 2312 Size: 61 1 156 188 87 5271 Fragments arranged by size: 5271 188 156 87 61 1 EcoRI G'AATT_C Cuts at: 3073 3451 3073 Size: 378 5386 FspI TGC'GCA Cuts at: 4270 4270 Size: 5764 HaeII r_GCGC'y Cuts at: 1678 4911 1678 Size: 3233 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2410 2442 2680 2712 2813 Size: 32 135 32 838 32 238 32 101 Cuts at: 2813 2834 2845 2866 4405 4437 4479 4511 1373 Size: 21 11 21 1539 32 42 32 2626 Fragments arranged by size: 2626 1539 838 238 135 101 42 32 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 3108 3354 669 Size: 988 281 1170 246 3079 Fragments arranged by size: 3079 1170 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5764 HpaI GTT'AAC Cuts at: 3354 3354 Size: 5764 KpnI G_GTAC'C Cuts at: 3089 3089 Size: 5764 MfeI C'AATT_G Cuts at: 3363 3363 Size: 5764 MluI A'CGCG_T Cuts at: 5640 5640 Size: 5764 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2485 2592 3017 4878 5062 1305 Size: 1180 107 425 1861 184 2007 Fragments arranged by size: 2007 1861 1180 425 184 107 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3942 Fragments arranged by size: 3942 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2951 3722 4081 4240 518 Size: 648 1785 771 359 159 2042 Fragments arranged by size: 2042 1785 771 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2546 3180 3857 5005 5250 1606 Size: 940 634 677 1148 245 2120 Fragments arranged by size: 2120 1148 940 677 634 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4273 Fragments arranged by size: 4273 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5764 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5764 NotI GC'GGCC_GC Cuts at: 3211 3211 Size: 5764 NspI r_CATG'y Cuts at: 1132 1229 3122 4667 1132 Size: 97 1893 1545 2229 Fragments arranged by size: 2229 1893 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5764 PciI A'CATG_T Cuts at: 4663 4663 Size: 5764 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5764 PfoI T'CCnGG_A Cuts at: 2788 2788 Size: 5764 PmeI GTTT'AAAC Cuts at: 3068 3068 Size: 5764 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2696 2728 3833 3865 5346 5378 2696 Size: 32 1105 32 1481 32 3082 Fragments arranged by size: 3082 1481 1105 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2312 2718 2805 2312 Size: 406 87 5271 Fragments arranged by size: 5271 406 87 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5764 PsiI TTA'TAA Cuts at: 3334 3334 Size: 5764 PspOMI G'GGCC_C Cuts at: 2373 2529 2373 Size: 156 5608 PstI C_TGCA'G Cuts at: 830 2382 3116 830 Size: 1552 734 3478 Fragments arranged by size: 3478 1552 734 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5764 PvuII CAG'CTG Cuts at: 2546 2546 Size: 5764 SacI G_AGCT'C Cuts at: 2589 2589 Size: 5764 SalI G'TCGA_C Cuts at: 1936 3106 1936 Size: 1170 4594 SanDI GG'GwC_CC Cuts at: 2312 2312 Size: 5764 SapI GCTCTTCn'nnn_ Cuts at: 2927 2927 Size: 5764 SbfI CC_TGCA'GG Cuts at: 3116 3116 Size: 5764 ScaI AGT'ACT Cuts at: 4012 5724 4012 Size: 1712 4052 SfcI C'TryA_G Cuts at: 826 1045 2378 3112 4247 4928 5119 826 Size: 219 1333 734 1135 681 191 1471 Fragments arranged by size: 1471 1333 1135 734 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5764 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5764 SmaI CCC'GGG Cuts at: 1872 3091 1872 Size: 1219 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3868 4769 5031 820 Size: 197 926 1925 901 262 1553 Fragments arranged by size: 1925 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3555 493 Size: 3062 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5764 SphI G_CATG'C Cuts at: 1132 3122 1132 Size: 1990 3774 SspI AAT'ATT Cuts at: 5 52 3688 5 Size: 47 3636 2081 Fragments arranged by size: 3636 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5764 StyI C'CwwG_G Cuts at: 513 1065 2004 2241 2368 2389 2809 3203 513 Size: 552 939 237 127 21 420 394 3074 Fragments arranged by size: 3074 939 552 420 394 237 127 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2551 2875 2950 3050 3791 3808 3961 Size: 1255 971 324 75 100 741 17 153 Cuts at: 3961 4146 4591 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 971 741 445 324 185 153 100 75 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4010 5722 96 Size: 274 80 33 51 1443 2033 1712 138 Fragments arranged by size: 2033 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2850 2882 5724 5756 2850 Size: 32 2842 32 2858 Fragments arranged by size: 2858 2842 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5764 XbaI T'CTAG_A Cuts at: 3100 3100 Size: 5764 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2903 2903 Size: 5764 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5764 XmaI C'CCGG_G Cuts at: 1870 3089 1870 Size: 1219 4545 XmnI GAAnn'nnTTC Cuts at: 3893 3893 Size: 5764 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3572 276 Size: 53 83 186 936 2038 2468 Fragments arranged by size: 2468 2038 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AscI BaeI BbeI BfrBI BplI BsiWI DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI