################################## # NO CUT ENZYMES ( 119 enzymes) # ################################## AatII AccI AccIII AclI AflII AgeI AhdI AlwNI Aor51HI ApaI AscI AseI AsnI Asp700I AspEI AspI AvaI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaWI BseAI BsiWI BsmBI BsoBI BspDI BspEI BspMI BsrBI BsrFI BssHII BssSI Bst1107I BstBI BstEII BstPI BstXI BstYI BstZ17I CelII CfoI Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI FseI FspI HaeII HhaI HinP1I HpaI KasI MamI MflI MluI MroI MslI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SalI SbfI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I StuI SwaI Tth111I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### Acc65I 1605 GGTACC G^GTACC AccII 97 CGCG CG^CG AcyI 494 GACGCC G[GA]^CG[CT]C AflIII 167 ACATGT A^C[GA][CT]GT Alw44I 383 GTGCAC G^TGCAC AocI 1501 CCTAAGG CC^T[ACGT]AGG ApaLI 383 GTGCAC G^TGCAC Asp718I 1605 GGTACC G^GTACC BalI 417 TGGCCA TGG^CCA BanI 1605 GGTACC G^G[CT][GA]CC BbsI 749 GTCTTC GAAGAC(2/6) BclI 1722 TGATCA T^GATCA BpuAI 749 GTCTTC GAAGAC(2/6) BsaHI 494 GACGCC G[GA]^CG[CT]C BsgI 468 CTGCAC GTGCAG(16/14) BsiEI 38 CGGTCG CG[GA][CT]^CG Bsp1407I 232 TGTACA T^GTACA BspLU11I 167 ACATGT A^CATGT BsrGI 232 TGTACA T^GTACA BstUI 97 CGCG CG^CG Bsu36I 1501 CCTAAGG CC^T[ACGT]AGG DraI 1615 TTTAAA TTT^AAA Eco81I 1501 CCTAAGG CC^T[ACGT]AGG EcoRV 103 GATATC GAT^ATC EcoT22I 622 ATGCAT ATGCA^T FbaI 1722 TGATCA T^GATCA HgaI 494 GACGC GACGC(5/10) Hin1I 494 GACGCC G[GA]^CG[CT]C HincII 1300 GTTGAC GT[CT]^[GA]AC HindII 1300 GTTGAC GT[CT]^[GA]AC KpnI 1605 GGTACC GGTAC^C KspI 96 CCGCGG CCGC^GG MaeIII 556 GTCAC ^GT[ACGT]AC MfeI 560 CAATTG C^AATTG MluNI 417 TGGCCA TGG^CCA MscI 417 TGGCCA TGG^CCA MunI 560 CAATTG C^AATTG MvnI 97 CGCG CG^CG NcoI 605 CCATGG C^CATGG NdeI 1027 CATATG CA^TATG NsiI 622 ATGCAT ATGCA^T PflMI 200 CCATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PleI 1159 GACTC GAGTC(4/5) PpuMI 1496 AGGACCC [GA]G^G[AT]CC[CT] SacI 1371 GAGCTC GAGCT^C SacII 96 CCGCGG CCGC^GG SapI 192 GCTCTTC GCTCTTC(1/4) ScaI 801 AGTACT AGT^ACT SexAI 71 ACCAGGT A^CC[AT]GGT SspBI 232 TGTACA T^GTACA SspI 1624 AATATT AAT^ATT Tsp45I 556 GTCAC ^GT[GC]AC Van91I 200 CCATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 797 CCAAAGTACTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AlwI 609 GGATC GGATC(4/5) 681 GGATC BanII 1310 GGGCTC G[GA]GC[CT]^C 1371 GAGCTC BcnI 78 CCGGG CC^[GC]GG 771 CCGGG BfaI 745 CTAG C^TAG 1090 CTAG BpmI 1691 CTCCAG CTGGAG(16/14) 393 CTGGAG BseRI 1010 GAGGAG GAGGAG(10/8) 1277 GAGGAG BstAPI 512 GCATCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 576 GCATTCTTTGC DraII 143 AGGGCCT [GA]G^G[ACGT]CC[CT] 1496 AGGACCC DsaI 96 CCGCGG C^C[GA][CT]GG 605 CCATGG EaeI 417 TGGCCA [CT]^GGCC[GA] 1213 CGGCCA EcoNI 346 CCTTGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1525 CCTCAAACAGG EcoO109I 143 AGGGCCT [GA]G^G[ACGT]CC[CT] 1496 AGGACCC EcoT14I 498 CCAAGG C^C[AT][AT]GG 605 CCATGG FauI 56 GCGGG CCCGC(4/6) 98 GCGGG HapII 78 CCGG C^CGG 771 CCGG HindIII 400 AAGCTT A^AGCTT 731 AAGCTT HpaII 78 CCGG C^CGG 771 CCGG MaeI 745 CTAG C^TAG 1090 CTAG MaeII 333 ACGT A^CGT 1446 ACGT MspI 78 CCGG C^CGG 771 CCGG NciI 78 CCGGG CC^[GC]GG 771 CCGGG PstI 1076 CTGCAG CTGCA^G 1772 CTGCAG SfcI 1076 CTGCAG C^T[GA][CT]AG 1772 CTGCAG StyI 498 CCAAGG C^C[AT][AT]GG 605 CCATGG TaqI 162 TCGA T^CGA 813 TCGA TthHB8I 162 TCGA T^CGA 813 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 59 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI AvaII BbvI BmyI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspHI BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII EarI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MboI MboII MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV NspI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI