(Circular) (Six-base) MAPSORT of: pFN21AB4048.seq Check: 6568 from: 1 to: 6493 pFN21AB4048 6493 bp With 182 enzymes: SgfI * August 16, 2010 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4303 278 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3836 1937 Size: 1899 4594 Acc65I G'GTAC_C Cuts at: 3610 3814 3610 Size: 204 6289 AclI AA'CG_TT Cuts at: 1563 4620 4993 1563 Size: 3057 373 3063 Fragments arranged by size: 3063 3057 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2741 3078 3189 3503 4556 5940 6208 1632 Size: 1109 337 111 314 1053 1384 268 1917 Fragments arranged by size: 1917 1384 1109 1053 337 314 268 111 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6296 AflIII A'CryG_T Cuts at: 1203 2172 5392 6369 1203 Size: 969 3220 977 1327 Fragments arranged by size: 3220 1327 977 969 AgeI A'CCGG_T Cuts at: 5324 5324 Size: 6493 AhdI GACnn_n'nnGTC Cuts at: 5222 5222 Size: 6493 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6493 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6493 AlwNI CAG_nnn'CTG Cuts at: 4236 5808 4236 Size: 1572 4921 ApaLI G'TGCA_C Cuts at: 2388 4550 5706 2388 Size: 2162 1156 3175 Fragments arranged by size: 3175 2162 1156 ApoI r'AATT_y Cuts at: 1493 3273 3438 3641 3737 3802 4028 4180 6289 Size: 1780 165 203 96 65 226 152 2109 Cuts at: 6289 1493 Size: 1697 Fragments arranged by size: 2109 1780 1697 226 203 165 152 96 65 AseI AT'TA_AT Cuts at: 160 1032 5047 160 Size: 872 4015 1606 Fragments arranged by size: 4015 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 AvaI C'yCGr_G Cuts at: 1870 1943 3818 1870 Size: 73 1875 4545 Fragments arranged by size: 4545 1875 73 BamHI G'GATC_C Cuts at: 3823 4190 3823 Size: 367 6126 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3610 3814 5269 618 Size: 325 217 627 1823 204 1455 1842 Fragments arranged by size: 1842 1823 1455 627 325 217 204 BanII G_rGCy'C Cuts at: 1391 3319 3380 3812 1391 Size: 1928 61 432 4072 Fragments arranged by size: 4072 1928 432 61 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2747 928 Size: 971 848 4674 Fragments arranged by size: 4674 971 848 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4684 4718 1903 Size: 34 50 34 2663 34 3678 Fragments arranged by size: 3678 2663 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4385 5595 4385 Size: 1210 5283 BclI T'GATC_A Cuts at: 1548 3727 1548 Size: 2179 4314 BfrBI ATG'CAT Cuts at: 2629 2629 Size: 6493 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5104 136 Size: 107 122 71 631 605 3432 1525 Fragments arranged by size: 3432 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6488 6488 Size: 6493 BlpI GC'TnA_GC Cuts at: 3910 3910 Size: 6493 Bme1580I G_kGCm'C Cuts at: 1790 2392 4554 5710 1790 Size: 602 2162 1156 2573 Fragments arranged by size: 2573 2162 1156 602 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6493 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4224 5182 442 Size: 416 53 83 399 24 2807 958 1753 Fragments arranged by size: 2807 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6493 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2419 3681 5153 1239 Size: 1180 1262 1472 2579 Fragments arranged by size: 2579 1472 1262 1180 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4618 5483 5781 4618 Size: 865 298 5330 Fragments arranged by size: 5330 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2223 3012 3360 5156 882 Size: 617 724 789 348 1796 2219 Fragments arranged by size: 2219 1796 789 724 617 348 BsaAI yAC'GTr Cuts at: 493 1204 4284 493 Size: 711 3080 2702 Fragments arranged by size: 3080 2702 711 BsaBI GATnn'nnATC Cuts at: 6487 6487 Size: 6493 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2500 4300 4682 5317 Size: 53 83 186 936 967 1800 382 635 Cuts at: 5317 275 Size: 1451 Fragments arranged by size: 1800 1451 967 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4926 5324 5598 5745 6156 4926 Size: 398 274 147 411 5263 Fragments arranged by size: 5263 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6268 Fragments arranged by size: 6268 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3030 3297 1186 Size: 1844 267 4382 Fragments arranged by size: 4382 1844 267 BseYI C'CCAG_C Cuts at: 5696 6192 5696 Size: 496 5997 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2458 1163 Size: 573 722 5198 Fragments arranged by size: 5198 722 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2046 3943 4704 5732 1540 Size: 275 186 45 1897 761 1028 2301 Fragments arranged by size: 2301 1897 1028 761 275 186 45 BsiHKAI G_wGCw'C Cuts at: 2392 2461 3330 3380 4554 4639 5710 2392 Size: 69 869 50 1174 85 1071 3175 Fragments arranged by size: 3175 1174 1071 869 85 69 50 BsmI GAATG_Cn' Cuts at: 2581 2629 2959 4004 4097 2581 Size: 48 330 1045 93 4977 Fragments arranged by size: 4977 1045 330 93 48 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2392 2461 3319 3330 3380 3812 4554 Size: 399 602 69 858 11 50 432 742 Cuts at: 4554 4639 5710 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 858 742 602 432 399 85 69 50 11 BspEI T'CCGG_A Cuts at: 6156 6156 Size: 6493 BspHI T'CATG_A Cuts at: 2112 2674 3112 4382 6112 2112 Size: 562 438 1270 1730 2493 Fragments arranged by size: 2493 1730 1270 562 438 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3848 844 Size: 3004 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4380 1394 Size: 429 2557 3507 Fragments arranged by size: 3507 2557 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2164 2718 2853 3261 4988 5162 65 Size: 1183 916 554 135 408 1727 174 1396 Fragments arranged by size: 1727 1396 1183 916 554 408 174 135 BsrFI r'CCGG_y Cuts at: 1700 1810 5137 5324 1700 Size: 110 3327 187 2869 Fragments arranged by size: 3327 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2237 96 Size: 2141 4352 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6493 BssSI C'ACGA_G Cuts at: 4553 5565 4553 Size: 1012 5481 BstAPI GCAn_nnn'nTGC Cuts at: 794 2523 2587 794 Size: 1729 64 4700 Fragments arranged by size: 4700 1729 64 BstBI TT'CG_AA Cuts at: 5297 5297 Size: 6493 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6493 BstYI r'GATC_y Cuts at: 1972 3823 4190 4575 4592 6044 6130 6142 6488 Size: 1851 367 385 17 1452 86 12 346 Cuts at: 6488 1972 Size: 1977 Fragments arranged by size: 1977 1851 1452 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3507 3507 Size: 6493 BtgI C'CryG_G Cuts at: 513 1065 2101 2610 513 Size: 552 1036 509 4396 Fragments arranged by size: 4396 1036 552 509 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5754 Fragments arranged by size: 5754 683 56 BtsI GCAGTG_nn' Cuts at: 4009 4803 4823 4009 Size: 794 20 5679 Fragments arranged by size: 5679 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6493 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6458 DraI TTT'AAA Cuts at: 3622 3797 3622 Size: 175 6318 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5500 809 Size: 507 4184 1802 Fragments arranged by size: 4184 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2422 3218 3940 Size: 54 1425 50 275 18 592 796 722 Cuts at: 3940 4829 6458 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 796 722 592 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3940 1537 Size: 275 2128 4090 Fragments arranged by size: 4090 2128 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2204 2782 3419 3479 4423 1353 Size: 851 578 637 60 944 3423 Fragments arranged by size: 3423 944 851 637 578 60 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5080 5454 5600 387 Size: 1064 138 3491 374 146 1280 Fragments arranged by size: 3491 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3378 3378 Size: 6493 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2419 2741 3078 3189 3503 3681 4556 Size: 393 787 322 337 111 314 178 875 Cuts at: 4556 5153 5940 6208 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 875 787 787 597 393 337 322 314 268 178 111 EcoNI CCTnn'n_nnAGG Cuts at: 2355 3534 2355 Size: 1179 5314 EcoO109I rG'GnC_Cy Cuts at: 2149 3502 2149 Size: 1353 5140 EcoRI G'AATT_C Cuts at: 3802 4180 3802 Size: 378 6115 EcoRV GAT'ATC Cuts at: 2110 2110 Size: 6493 FspI TGC'GCA Cuts at: 4999 4999 Size: 6493 HaeII r_GCGC'y Cuts at: 1678 5640 1678 Size: 3962 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2338 2370 2606 2638 2671 Size: 32 135 32 766 32 236 32 33 Cuts at: 2671 2703 2915 2947 5134 5166 5208 5240 1373 Size: 32 212 32 2187 32 42 32 2626 Fragments arranged by size: 2626 2187 766 236 212 135 42 33 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3307 3837 4083 669 Size: 988 281 1369 530 246 3079 Fragments arranged by size: 3079 1369 988 530 281 246 HindIII A'AGCT_T Cuts at: 748 2405 2736 748 Size: 1657 331 4505 Fragments arranged by size: 4505 1657 331 HpaI GTT'AAC Cuts at: 4083 4083 Size: 6493 KpnI G_GTAC'C Cuts at: 3614 3818 3614 Size: 204 6289 MfeI C'AATT_G Cuts at: 2565 4092 2565 Size: 1527 4966 MluI A'CGCG_T Cuts at: 6369 6369 Size: 6493 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2235 5607 5791 1305 Size: 930 3372 184 2007 Fragments arranged by size: 3372 2007 930 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2424 10 Size: 54 1425 343 592 4079 Fragments arranged by size: 4079 1425 592 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 4451 4810 4969 518 Size: 648 3285 359 159 2042 Fragments arranged by size: 3285 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2061 2103 2397 3909 4586 5734 5979 1606 Size: 455 42 294 1512 677 1148 245 2120 Fragments arranged by size: 2120 1512 1148 677 455 294 245 42 NcoI C'CATG_G Cuts at: 513 1065 2610 513 Size: 552 1545 4396 Fragments arranged by size: 4396 1545 552 NdeI CA'TA_TG Cuts at: 387 3033 387 Size: 2646 3847 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6493 NotI GC'GGCC_GC Cuts at: 3940 3940 Size: 6493 NsiI A_TGCA'T Cuts at: 2631 2631 Size: 6493 NspI r_CATG'y Cuts at: 1132 1229 2176 2918 3068 3202 3851 5396 1132 Size: 97 947 742 150 134 649 1545 2229 Fragments arranged by size: 2229 1545 947 742 649 150 134 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6493 PciI A'CATG_T Cuts at: 2172 5392 2172 Size: 3220 3273 PflMI CCAn_nnn'nTGG Cuts at: 1108 2211 1108 Size: 1103 5390 PfoI T'CCnGG_A Cuts at: 2356 2356 Size: 6493 PmeI GTTT'AAAC Cuts at: 3797 3797 Size: 6493 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4562 4594 6075 6107 4562 Size: 32 1481 32 4948 Fragments arranged by size: 4948 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3502 3502 Size: 6493 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6493 PsiI TTA'TAA Cuts at: 4063 4063 Size: 6493 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2799 2831 2799 Size: 32 6461 PstI C_TGCA'G Cuts at: 830 3085 3781 3845 830 Size: 2255 696 64 3478 Fragments arranged by size: 3478 2255 696 64 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6493 SacI G_AGCT'C Cuts at: 3380 3380 Size: 6493 SacII CC_GC'GG Cuts at: 2104 2104 Size: 6493 SalI G'TCGA_C Cuts at: 1936 3835 1936 Size: 1899 4594 SapI GCTCTTCn'nnn_ Cuts at: 2204 2204 Size: 6493 SbfI CC_TGCA'GG Cuts at: 3845 3845 Size: 6493 ScaI AGT'ACT Cuts at: 2808 4741 6453 2808 Size: 1933 1712 2848 Fragments arranged by size: 2848 1933 1712 SexAI A'CCwGG_T Cuts at: 2076 2076 Size: 6493 SfcI C'TryA_G Cuts at: 826 1045 3081 3777 3841 4976 5657 5848 826 Size: 219 2036 696 64 1135 681 191 1471 Fragments arranged by size: 2036 1471 1135 696 681 219 191 64 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6493 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6493 SmaI CCC'GGG Cuts at: 1872 3820 1872 Size: 1948 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4597 5498 5760 820 Size: 197 926 2654 901 262 1553 Fragments arranged by size: 2654 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4284 493 Size: 3791 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6493 SphI G_CATG'C Cuts at: 1132 3851 1132 Size: 2719 3774 SspI AAT'ATT Cuts at: 5 52 3631 4417 5 Size: 47 3579 786 2081 Fragments arranged by size: 3579 2081 786 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6493 StyI C'CwwG_G Cuts at: 513 1065 2503 2610 3932 513 Size: 552 1438 107 1322 3074 Fragments arranged by size: 3074 1438 1322 552 107 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2496 4520 4537 4690 4875 5320 325 Size: 1255 916 2024 17 153 185 445 1498 Fragments arranged by size: 2024 1498 1255 916 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2237 2806 4739 Size: 274 80 33 51 1443 260 569 1933 Cuts at: 4739 6451 96 Size: 1712 138 Fragments arranged by size: 1933 1712 1443 569 274 260 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6453 6485 6453 Size: 32 6461 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6493 XbaI T'CTAG_A Cuts at: 3829 3829 Size: 6493 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2809 2809 Size: 6493 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6493 XmaI C'CCGG_G Cuts at: 1870 3818 1870 Size: 1948 4545 XmnI GAAnn'nnTTC Cuts at: 4622 4622 Size: 6493 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4301 276 Size: 53 83 186 936 2767 2468 Fragments arranged by size: 2767 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PvuII RsrII SanDI SfiI SfoI SrfI SwaI