################################## # NO CUT ENZYMES ( 169 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmI BsoBI Bsp1286I BspDI BspEI BspHI BsrBI BsrDI BsrI BssHII BssSI BstAPI BstEII BstPI BstUI BstXI Bsu36I CelII CfoI ClaI CpoI DraI DraII DraIII DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRV EcoT22I FauI FseI FspI HaeII HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MaeII MluI MluNI MroI MscI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AccI 375 GTATAC GT^[AC][GT]AC AflIII 371 ACATGT A^C[GA][CT]GT AvaII 313 GGACC G^G[AT]CC BamHI 927 GGATCC G^GATCC BclI 500 TGATCA T^GATCA BsiYI 683 CCATATCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 683 CCATATCCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmFI 443 GGGAC GGGAC(10/14) Bsp1407I 455 TGTACA T^GTACA BspLU11I 371 ACATGT A^CATGT BspMI 787 ACCTGC ACCTGC(4/8) BsrFI 135 GCCGGT [GA]^CCGG[CT] BsrGI 455 TGTACA T^GTACA Bst1107I 375 GTATAC GTA^TAC BstBI 287 TTCGAA TT^CGAA BstZ17I 375 GTATAC GTA^TAC Cfr10I 135 GCCGGT [GA]^CCGG[CT] DdeI 837 CTTAG C^T[ACGT]AG DrdI 121 GACACAATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 815 GAATTC G^AATTC FbaI 500 TGATCA T^GATCA HapII 136 CCGG C^CGG HpaII 136 CCGG C^CGG MfeI 125 CAATTG C^AATTG MseI 584 TTAA T^TAA MspA1I 633 CAGCTG C[AC]G^C[GT]G MspI 136 CCGG C^CGG MunI 125 CAATTG C^AATTG NlaIV 927 GGATCC GG[ACGT]^[ACGT]CC NspI 371 ACATGT [GA]CATG^[CT] NspV 287 TTCGAA TT^CGAA PstI 339 CTGCAG CTGCA^G PvuII 633 CAGCTG CAG^CTG SfcI 339 CTGCAG C^T[GA][CT]AG SfuI 287 TTCGAA TT^CGAA SspBI 455 TGTACA T^GTACA TaqI 288 TCGA T^CGA Tru9I 584 TTAA T^TAA TthHB8I 288 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcsI 115 GAATTT [GA]^AATT[CT] 815 GAATTC ApoI 115 GAATTT [GA]^AATT[CT] 815 GAATTC BbvI 42 GCTGC GCAGC(8/12) 635 GCTGC BsaBI 412 GATCGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 660 GATTCTGATC BsaJI 316 CCTTGG C^C[ACGT][ACGT]GG 393 CCTTGG BstYI 194 GGATCT [GA]^GATC[CT] 927 GGATCC Cfr13I 85 GGGCC G^G[ACGT]CC 313 GGACC EaeI 48 CGGCCA [CT]^GGCC[GA] 647 TGGCCG EcoT14I 316 CCTTGG C^C[AT][AT]GG 393 CCTTGG Fnu4HI 42 GCTGC GC^[ACGT]GC 635 GCTGC HgaI 15 GACGC GACGC(5/10) 270 GACGC ItaI 42 GCTGC GC^[ACGT]GC 635 GCTGC MamI 412 GATCGCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 660 GATTCTGATC MflI 194 GGATCT [GA]^GATC[CT] 927 GGATCC MnlI 261 CCTC CCTC(7/6) 556 GAGG MslI 621 CACCTATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1065 CACTGATGTG MwoI 71 GCAACAGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 407 GCATTGATCGC PleI 445 GACTC GAGTC(4/5) 57 GAGTC Sau96I 85 GGGCC G^G[ACGT]CC 313 GGACC StyI 316 CCTTGG C^C[AT][AT]GG 393 CCTTGG TfiI 660 GATTC G^A[AT]TC 856 GAATC TseI 42 GCTGC G^C[AT]GC 635 GCTGC XhoII 194 GGATCT [GA]^GATC[CT] 927 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AfaI AluI AlwI BsgI BssKI BstF5I BstNI Cac8I DpnII EcoRII FokI HaeIII HinfI HphI MaeIII MboI MboII MvaI NdeII NlaIII RsaI Sau3AI ScrFI SfaNI Tsp45I Tsp509I TspRI