################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## AatII Acc65I AccIII AclI AcsI AflII AgeI AhdI Alw44I AlwI AocI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AviII AvrII BamHI BbrPI BbvCI BciVI BclI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaI BseAI BseRI BsiHKAI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspEI BspMI BsrBI BsrDI BsrGI BssHII Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstXI BstYI BstZ17I Bsu36I CelII ClaI CpoI DdeI DraI DrdI Eam1105I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FspI HindIII HpaI KpnI MamI MfeI MflI MluI MroI MunI NcoI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RsrII SacI SbfI ScaI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tsp509I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccI 646 GTCGAC GT^[AC][GT]AC AfaI 714 GTAC GT^AC AflIII 961 ACATGT A^C[GA][CT]GT Aor51HI 28 AGCGCT AGC^GCT AvaI 165 CTCGGG C^[CT]CG[GA]G AvaII 536 GGTCC G^G[AT]CC BalI 675 TGGCCA TGG^CCA BbeI 346 GGCGCC GGCGC^C BcgI 774 CGAGAACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 616 CTAG C^TAG BsaWI 240 TCCGGT [AT]^CCGG[AT] BsiEI 98 CGGCCG CG[GA][CT]^CG BsoBI 165 CTCGGG C^[CT]CG[GA]G BspLU11I 961 ACATGT A^CATGT BssSI 214 CACGAG CACGAG(-5/-1) DpnII 162 GATC ^GATC DraIII 292 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 98 CGGCCG C^GGCCG EarI 229 CTCTTC CTCTTC(1/4) EclXI 98 CGGCCG C^GGCCG Eco47III 28 AGCGCT AGC^GCT Eco52I 98 CGGCCG C^GGCCG EcoT14I 936 CCTTGG C^C[AT][AT]GG FseI 189 GGCCGGCC GGCCGG^CC HgaI 354 GACGC GACGC(5/10) HincII 646 GTCGAC GT[CT]^[GA]AC HindII 646 GTCGAC GT[CT]^[GA]AC KasI 346 GGCGCC G^GCGCC Ksp632I 229 CTCTTC CTCTTC(1/4) MaeI 616 CTAG C^TAG MaeII 337 ACGT A^CGT MboI 162 GATC ^GATC MluNI 675 TGGCCA TGG^CCA MscI 675 TGGCCA TGG^CCA MseI 629 TTAA T^TAA NaeI 190 GCCGGC GCC^GGC NarI 346 GGCGCC GG^CGCC NdeI 477 CATATG CA^TATG NdeII 162 GATC ^GATC NgoMIV 190 GCCGGC G^CCGGC NspI 961 ACATGT [GA]CATG^[CT] PflMI 505 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 535 GGGTCCC [GA]G^G[AT]CC[CT] RsaI 714 GTAC GT^AC SalI 646 GTCGAC G^TCGAC SapI 228 GCTCTTC GCTCTTC(1/4) Sau3AI 162 GATC ^GATC SexAI 293 ACCTGGT A^CC[AT]GGT SfoI 346 GGCGCC GGC^GCC StyI 936 CCTTGG C^C[AT][AT]GG Tru9I 629 TTAA T^TAA TspRI 277 CAGTG CA[GC]TG[ACGT][ACGT]^ Van91I 505 CCATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccII 138 CGCG CG^CG 150 CGCG AcyI 346 GGCGCC G[GA]^CG[CT]C 354 GACGCC AlwNI 638 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG 879 CAGCGACTG ApaI 123 GGGCCC GGGCC^C 168 GGGCCC AspI 253 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC 604 GACATCGTC BanII 123 GGGCCC G[GA]GC[CT]^C 168 GGGCCC BbsI 472 GAAGAC GAAGAC(2/6) 601 GAAGAC BglI 148 GCCGCGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 170 GCCCCGGCGGC BpmI 697 CTGGAG CTGGAG(16/14) 793 CTGGAG BpuAI 472 GAAGAC GAAGAC(2/6) 601 GAAGAC BsaHI 346 GGCGCC G[GA]^CG[CT]C 354 GACGCC BsgI 445 CTGCAC GTGCAG(16/14) 339 GTGCAG BspHI 849 TCATGA T^CATGA 872 TCATGA BsrI 650 ACTGG ACTGG(1/-1) 792 ACTGG BstUI 138 CGCG CG^CG 150 CGCG HaeII 28 AGCGCT [GA]GCGC^[CT] 346 GGCGCC Hin1I 346 GGCGCC G[GA]^CG[CT]C 354 GACGCC HinfI 846 GATTC G^A[ACGT]TC 941 GAATC HphI 159 GGTGA GGTGA(8/7) 654 GGTGA KspI 137 CCGCGG CCGC^GG 149 CCGCGG MaeIII 302 GTAAC ^GT[ACGT]AC 949 GTTAC MslI 687 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 850 CATGACGGTG MspA1I 137 CCGCGG C[AC]G^C[GT]G 149 CCGCGG MvnI 138 CGCG CG^CG 150 CGCG PflFI 253 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC 604 GACATCGTC PstI 42 CTGCAG CTGCA^G 274 CTGCAG RcaI 849 TCATGA T^CATGA 872 TCATGA SacII 137 CCGCGG CCGC^GG 149 CCGCGG SfaNI 660 GATGC GCATC(5/9) 362 GCATC TfiI 846 GATTC G^A[AT]TC 941 GAATC Tth111I 253 GACTATGTC GAC[ACGT]^[ACGT][ACGT]GTC 604 GACATCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI BanI BbvI BcnI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BsrFI BssKI BstNI Cac8I CfoI Cfr10I Cfr13I DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MboII MnlI MspI MvaI MwoI NciI NlaIII NlaIV Sau96I ScrFI SfcI TaqI TseI TthHB8I