(Circular) (Six-base) MAPSORT of: pFN21AE4205.seq Check: 1717 from: 1 to: 6874 pFN21AE4205 6874 bp With 182 enzymes: SgfI * March 28, 2012 13:18 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2126 2771 2126 Size: 645 6229 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4684 278 Size: 53 83 186 936 3148 2468 Fragments arranged by size: 3148 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4217 1937 Size: 2280 4594 Acc65I G'GTAC_C Cuts at: 4195 4195 Size: 6874 AclI AA'CG_TT Cuts at: 1563 5001 5374 1563 Size: 3438 373 3063 Fragments arranged by size: 3438 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3001 3149 3326 3613 4160 4937 6321 6589 Size: 1369 148 177 287 547 777 1384 268 Cuts at: 6589 1632 Size: 1917 Fragments arranged by size: 1917 1384 1369 777 547 287 268 177 148 AfeI AGC'GCT Cuts at: 2654 2753 2654 Size: 99 6775 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6677 AflIII A'CryG_T Cuts at: 1203 3875 4029 5773 6750 1203 Size: 2672 154 1744 977 1327 Fragments arranged by size: 2672 1744 1327 977 154 AgeI A'CCGG_T Cuts at: 5705 5705 Size: 6874 AhdI GACnn_n'nnGTC Cuts at: 3748 5603 3748 Size: 1855 5019 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6874 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2474 2627 1184 Size: 1290 153 5431 Fragments arranged by size: 5431 1290 153 AlwNI CAG_nnn'CTG Cuts at: 3841 4617 6189 3841 Size: 776 1572 4526 Fragments arranged by size: 4526 1572 776 ApaI G_GGCC'C Cuts at: 2709 3486 2709 Size: 777 6097 ApaLI G'TGCA_C Cuts at: 3533 4931 6087 3533 Size: 1398 1156 4320 Fragments arranged by size: 4320 1398 1156 ApoI r'AATT_y Cuts at: 1493 3074 3911 3969 4183 4409 4561 6670 1493 Size: 1581 837 58 214 226 152 2109 1697 Fragments arranged by size: 2109 1697 1581 837 226 214 152 58 AseI AT'TA_AT Cuts at: 160 1032 5428 160 Size: 872 4396 1606 Fragments arranged by size: 4396 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6874 AvaI C'yCGr_G Cuts at: 1870 1943 2621 4199 1870 Size: 73 678 1578 4545 Fragments arranged by size: 4545 1578 678 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2370 2403 2370 Size: 33 6841 BamHI G'GATC_C Cuts at: 4204 4571 4204 Size: 367 6507 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4195 5650 618 Size: 325 217 627 2408 1455 1842 Fragments arranged by size: 2408 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2186 2709 3471 3486 3682 3960 4193 1391 Size: 795 523 762 15 196 278 233 4072 Fragments arranged by size: 4072 795 762 523 278 233 196 15 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3815 3894 928 Size: 971 1916 79 3908 Fragments arranged by size: 3908 1916 971 79 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5065 5099 1903 Size: 34 3128 34 3678 Fragments arranged by size: 3678 3128 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4766 5976 4766 Size: 1210 5664 BclI T'GATC_A Cuts at: 1548 1548 Size: 6874 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5485 136 Size: 107 122 71 631 605 3813 1525 Fragments arranged by size: 3813 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2868 6869 2868 Size: 4001 2873 BlpI GC'TnA_GC Cuts at: 4291 4291 Size: 6874 Bme1580I G_kGCm'C Cuts at: 1790 2709 3432 3486 3537 4935 6091 1790 Size: 919 723 54 51 1398 1156 2573 Fragments arranged by size: 2573 1398 1156 919 723 54 51 BmgBI CAC'GTC Cuts at: 1318 2830 3892 1318 Size: 1512 1062 4300 Fragments arranged by size: 4300 1512 1062 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2376 2564 3676 Size: 416 53 83 399 24 959 188 1112 Cuts at: 3676 4605 5563 442 Size: 929 958 1753 Fragments arranged by size: 1753 1112 959 958 929 416 399 188 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6874 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3683 3748 3756 4016 4145 5534 1239 Size: 2444 65 8 260 129 1389 2579 Fragments arranged by size: 2579 2444 1389 260 129 65 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2663 2777 3131 4999 5864 6162 2663 Size: 114 354 1868 865 298 3375 Fragments arranged by size: 3375 1868 865 354 298 114 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5537 882 Size: 617 4038 2219 Fragments arranged by size: 4038 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3427 4030 4665 493 Size: 711 2223 603 635 2702 Fragments arranged by size: 2702 2223 711 635 603 BsaBI GATnn'nnATC Cuts at: 2873 6868 2873 Size: 3995 2879 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2641 2988 4681 5063 Size: 53 83 186 936 1108 347 1693 382 Cuts at: 5063 5698 275 Size: 635 1451 Fragments arranged by size: 1693 1451 1108 936 635 382 347 186 83 53 BsaWI w'CCGG_w Cuts at: 5307 5705 5979 6126 6537 5307 Size: 398 274 147 411 5644 Fragments arranged by size: 5644 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2684 2714 2978 3008 3919 Size: 30 165 30 1281 30 264 30 911 Cuts at: 3919 3949 1178 Size: 30 4103 Fragments arranged by size: 4103 1281 911 264 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2335 2689 3490 3604 3924 1186 Size: 1149 354 801 114 320 4136 Fragments arranged by size: 4136 1149 801 354 320 114 BseYI C'CCAG_C Cuts at: 6077 6573 6077 Size: 496 6378 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2098 2152 2227 2450 2470 2618 2917 Size: 573 362 54 75 223 20 148 299 Cuts at: 2917 3395 3986 1163 Size: 478 591 4051 Fragments arranged by size: 4051 591 573 478 362 299 223 148 75 54 20 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2581 4324 5085 6113 1540 Size: 275 186 580 1743 761 1028 2301 Fragments arranged by size: 2301 1743 1028 761 580 275 186 BsiHKAI G_wGCw'C Cuts at: 2670 3471 3537 3960 4935 5020 6091 2670 Size: 801 66 423 975 85 1071 3453 Fragments arranged by size: 3453 1071 975 801 423 85 66 BsmI GAATG_Cn' Cuts at: 3159 3326 4385 4478 3159 Size: 167 1059 93 5555 Fragments arranged by size: 5555 1059 167 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2648 2648 Size: 6874 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2186 2670 2709 3432 3471 3486 3537 Size: 399 396 484 39 723 39 15 51 Cuts at: 3537 3682 3960 4193 4935 5020 6091 1391 Size: 145 278 233 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 723 484 399 396 278 233 145 85 51 39 39 15 BspEI T'CCGG_A Cuts at: 6537 6537 Size: 6874 BspHI T'CATG_A Cuts at: 4763 6493 4763 Size: 1730 5144 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2126 2640 2771 4229 844 Size: 1282 514 131 1458 3489 Fragments arranged by size: 3489 1458 1282 514 131 BsrBI CCG'CTC Cuts at: 1394 1823 2107 2146 2583 2697 4761 1394 Size: 429 284 39 437 114 2064 3507 Fragments arranged by size: 3507 2064 437 429 284 114 39 BsrDI GCAATG_nn' Cuts at: 65 1248 2920 4076 5369 5543 65 Size: 1183 1672 1156 1293 174 1396 Fragments arranged by size: 1672 1396 1293 1183 1156 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2454 5518 5705 1700 Size: 110 644 3064 187 2869 Fragments arranged by size: 3064 2869 644 187 110 BsrGI T'GTAC_A Cuts at: 96 2039 3754 96 Size: 1943 1715 3216 Fragments arranged by size: 3216 1943 1715 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6874 BssSI C'ACGA_G Cuts at: 4934 5946 4934 Size: 1012 5862 BstAPI GCAn_nnn'nTGC Cuts at: 794 2593 2763 2787 2940 794 Size: 1799 170 24 153 4728 Fragments arranged by size: 4728 1799 170 153 24 BstBI TT'CG_AA Cuts at: 5678 5678 Size: 6874 BstEII G'GTnAC_C Cuts at: 1184 2539 1184 Size: 1355 5519 BstXI CCAn_nnnn'nTGG Cuts at: 3349 3349 Size: 6874 BstYI r'GATC_y Cuts at: 1972 2868 3345 4204 4571 4956 4973 6425 6511 Size: 896 477 859 367 385 17 1452 86 Cuts at: 6511 6523 6869 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 896 859 477 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2327 3502 2327 Size: 1175 5699 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5383 Fragments arranged by size: 5383 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2725 3010 487 Size: 683 56 1499 285 4351 Fragments arranged by size: 4351 1499 683 285 56 BtsI GCAGTG_nn' Cuts at: 2164 2587 4390 5184 5204 2164 Size: 423 1803 794 20 3834 Fragments arranged by size: 3834 1803 794 423 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6874 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2420 2455 529 Size: 35 1856 35 4948 Fragments arranged by size: 4948 1856 35 35 DraI TTT'AAA Cuts at: 3100 4036 4178 3100 Size: 936 142 5796 Fragments arranged by size: 5796 936 142 DraIII CAC_nnn'GTG Cuts at: 2425 2425 Size: 6874 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5881 809 Size: 507 4565 1802 Fragments arranged by size: 4565 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2814 2962 3316 Size: 54 1425 50 275 18 984 148 354 Cuts at: 3316 4321 5210 6839 8 Size: 1005 889 1629 43 Fragments arranged by size: 1629 1425 1005 984 889 354 275 148 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4321 1537 Size: 275 2509 4090 Fragments arranged by size: 4090 2509 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3020 3476 4804 1353 Size: 1667 456 1328 3423 Fragments arranged by size: 3423 1667 1328 456 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5461 5835 5981 387 Size: 1064 138 3872 374 146 1280 Fragments arranged by size: 3872 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3469 3958 3469 Size: 489 6385 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3001 3149 3326 3613 3683 3748 3756 Size: 393 1369 148 177 287 70 65 8 Cuts at: 3756 4016 4145 4160 4937 5534 6321 6589 1239 Size: 260 129 15 777 597 787 268 1524 Fragments arranged by size: 1524 1369 787 777 597 393 287 268 260 177 148 129 70 65 15 8 EcoNI CCTnn'n_nnAGG Cuts at: 2717 2717 Size: 6874 EcoO109I rG'GnC_Cy Cuts at: 3482 4132 3482 Size: 650 6224 EcoRI G'AATT_C Cuts at: 3074 3911 4183 4561 3074 Size: 837 272 378 5387 Fragments arranged by size: 5387 837 378 272 EcoRV GAT'ATC Cuts at: 4081 4081 Size: 6874 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2110 2704 2110 Size: 594 6280 FspI TGC'GCA Cuts at: 2363 3155 5380 2363 Size: 792 2225 3857 Fragments arranged by size: 3857 2225 792 HaeII r_GCGC'y Cuts at: 1678 2656 2689 2755 2850 3024 6021 1678 Size: 978 33 66 95 174 2997 2531 Fragments arranged by size: 2997 2531 978 174 95 66 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2041 2073 2684 2716 2978 Size: 32 135 32 469 32 611 32 262 Cuts at: 2978 3010 3187 3219 3262 3294 3734 3766 3881 Size: 32 177 32 43 32 440 32 115 Cuts at: 3881 3913 5515 5547 5589 5621 1373 Size: 32 1602 32 42 32 2626 Fragments arranged by size: 2626 1602 611 469 440 262 177 135 115 43 42 32 32 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 4218 4464 669 Size: 988 281 2280 246 3079 Fragments arranged by size: 3079 2280 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6874 HpaI GTT'AAC Cuts at: 4464 4464 Size: 6874 KpnI G_GTAC'C Cuts at: 4199 4199 Size: 6874 MfeI C'AATT_G Cuts at: 2437 3822 4473 2437 Size: 1385 651 4838 Fragments arranged by size: 4838 1385 651 MluI A'CGCG_T Cuts at: 6750 6750 Size: 6874 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5988 6172 1305 Size: 4683 184 2007 Fragments arranged by size: 4683 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2816 2964 3318 10 Size: 54 1425 343 984 148 354 3566 Fragments arranged by size: 3566 1425 984 354 343 148 54 MslI CAynn'nnrTG Cuts at: 518 1166 2808 3260 4832 5191 5350 518 Size: 648 1642 452 1572 359 159 2042 Fragments arranged by size: 2042 1642 1572 648 452 359 159 MspA1I CmG'CkG Cuts at: 1606 2286 2521 2617 4290 4967 6115 6360 1606 Size: 680 235 96 1673 677 1148 245 2120 Fragments arranged by size: 2120 1673 1148 680 677 245 235 96 NaeI GCC'GGC Cuts at: 2456 2456 Size: 6874 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5383 Fragments arranged by size: 5383 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6874 NgoMIV G'CCGG_C Cuts at: 2454 2454 Size: 6874 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6874 NotI GC'GGCC_GC Cuts at: 4321 4321 Size: 6874 NspI r_CATG'y Cuts at: 1132 1229 2596 2662 3879 4232 5777 1132 Size: 97 1367 66 1217 353 1545 2229 Fragments arranged by size: 2229 1545 1367 1217 353 97 66 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6874 PciI A'CATG_T Cuts at: 3875 5773 3875 Size: 1898 4976 PflMI CCAn_nnn'nTGG Cuts at: 1108 3841 1108 Size: 2733 4141 PmeI GTTT'AAAC Cuts at: 4178 4178 Size: 6874 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4943 4975 6456 6488 4943 Size: 32 1481 32 5329 Fragments arranged by size: 5329 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6874 PsiI TTA'TAA Cuts at: 4444 4444 Size: 6874 PspOMI G'GGCC_C Cuts at: 2705 3482 2705 Size: 777 6097 PstI C_TGCA'G Cuts at: 830 2526 3336 4226 830 Size: 1696 810 890 3478 Fragments arranged by size: 3478 1696 890 810 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6874 PvuII CAG'CTG Cuts at: 2521 2617 2521 Size: 96 6778 SacI G_AGCT'C Cuts at: 3471 3960 3471 Size: 489 6385 SalI G'TCGA_C Cuts at: 1936 4216 1936 Size: 2280 4594 SapI GCTCTTCn'nnn_ Cuts at: 3476 3476 Size: 6874 SbfI CC_TGCA'GG Cuts at: 4226 4226 Size: 6874 ScaI AGT'ACT Cuts at: 2906 5122 6834 2906 Size: 2216 1712 2946 Fragments arranged by size: 2946 2216 1712 SexAI A'CCwGG_T Cuts at: 2421 2421 Size: 6874 SfcI C'TryA_G Cuts at: 826 1045 2522 2599 3332 3385 4222 5357 6038 Size: 219 1477 77 733 53 837 1135 681 Cuts at: 6038 6229 826 Size: 191 1471 Fragments arranged by size: 1477 1471 1135 837 733 681 219 191 77 53 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6874 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6874 SmaI CCC'GGG Cuts at: 1872 4201 1872 Size: 2329 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2678 2792 3146 4978 5879 6141 Size: 197 926 735 114 354 1832 901 262 Cuts at: 6141 820 Size: 1553 Fragments arranged by size: 1832 1553 926 901 735 354 262 197 114 SnaBI TAC'GTA Cuts at: 493 4665 493 Size: 4172 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6874 SphI G_CATG'C Cuts at: 1132 2596 4232 1132 Size: 1464 1636 3774 Fragments arranged by size: 3774 1636 1464 SspI AAT'ATT Cuts at: 5 52 4798 5 Size: 47 4746 2081 Fragments arranged by size: 4746 2081 47 StuI AGG'CCT Cuts at: 1517 2798 3391 3668 1517 Size: 1281 593 277 4723 Fragments arranged by size: 4723 1281 593 277 StyI C'CwwG_G Cuts at: 513 1065 2004 3294 3432 3651 3861 4313 513 Size: 552 939 1290 138 219 210 452 3074 Fragments arranged by size: 3074 1290 939 552 452 219 210 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2898 3590 4901 4918 5071 5256 5701 Size: 1255 1318 692 1311 17 153 185 445 Cuts at: 5701 325 Size: 1498 Fragments arranged by size: 1498 1318 1311 1255 692 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2039 2904 3754 Size: 274 80 33 51 1443 62 865 850 Cuts at: 3754 5120 6832 96 Size: 1366 1712 138 Fragments arranged by size: 1712 1443 1366 865 850 274 138 80 62 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2870 2902 6834 6866 2870 Size: 32 3932 32 2878 Fragments arranged by size: 3932 2878 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2828 1316 Size: 1512 5362 XbaI T'CTAG_A Cuts at: 3205 4210 3205 Size: 1005 5869 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2040 2419 3349 3658 2040 Size: 379 930 309 5256 Fragments arranged by size: 5256 930 379 309 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6874 XmaI C'CCGG_G Cuts at: 1870 4199 1870 Size: 2329 4545 XmnI GAAnn'nnTTC Cuts at: 2552 4169 5003 2552 Size: 1617 834 4423 Fragments arranged by size: 4423 1617 834 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4682 276 Size: 53 83 186 936 3148 2468 Fragments arranged by size: 3148 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI