(Linear) (Six-base) MAPSORT of: KIEE4205.seq Check: 6381 from: 1 to: 2169 KIEE4205.orf With 182 enzymes: SgfI * March 22, 2012 17:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 121 766 2169 Size: 121 645 1403 Fragments arranged by size: 1403 645 121 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 996 1144 1321 1608 2155 2169 Size: 996 148 177 287 547 14 Fragments arranged by size: 996 547 287 177 148 14 AfeI AGC'GCT Cuts at: 0 649 748 2169 Size: 649 99 1421 Fragments arranged by size: 1421 649 99 AflIII A'CryG_T Cuts at: 0 1870 2024 2169 Size: 1870 154 145 Fragments arranged by size: 1870 154 145 AhdI GACnn_n'nnGTC Cuts at: 0 1743 2169 Size: 1743 426 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 469 622 2169 Size: 469 153 1547 Fragments arranged by size: 1547 469 153 AlwNI CAG_nnn'CTG Cuts at: 0 1836 2169 Size: 1836 333 ApaI G_GGCC'C Cuts at: 0 704 1481 2169 Size: 704 777 688 Fragments arranged by size: 777 704 688 ApaLI G'TGCA_C Cuts at: 0 1528 2169 Size: 1528 641 ApoI r'AATT_y Cuts at: 0 1069 1906 1964 2169 Size: 1069 837 58 205 Fragments arranged by size: 1069 837 205 58 AvaI C'yCGr_G Cuts at: 0 616 2169 Size: 616 1553 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 0 365 398 2169 Size: 365 33 1771 Fragments arranged by size: 1771 365 33 BanII G_rGCy'C Cuts at: 0 181 704 1466 1481 1677 1955 2169 Size: 181 523 762 15 196 278 214 Fragments arranged by size: 762 523 278 214 196 181 15 BbsI GAAGACnn'nnnn_ Cuts at: 0 1810 1889 2169 Size: 1810 79 280 Fragments arranged by size: 1810 280 79 BglII A'GATC_T Cuts at: 0 863 2169 Size: 863 1306 Bme1580I G_kGCm'C Cuts at: 0 704 1427 1481 1532 2169 Size: 704 723 54 51 637 Fragments arranged by size: 723 704 637 54 51 BmgBI CAC'GTC Cuts at: 0 825 1887 2169 Size: 825 1062 282 Fragments arranged by size: 1062 825 282 BmrI ACTGGGnnnn_n' Cuts at: 0 371 559 1671 2169 Size: 371 188 1112 498 Fragments arranged by size: 1112 498 371 188 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1678 1743 1751 2011 2140 2169 Size: 1678 65 8 260 129 29 Fragments arranged by size: 1678 260 129 65 29 8 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 658 772 1126 2169 Size: 658 114 354 1043 Fragments arranged by size: 1043 658 354 114 BsaAI yAC'GTr Cuts at: 0 1422 2025 2169 Size: 1422 603 144 Fragments arranged by size: 1422 603 144 BsaBI GATnn'nnATC Cuts at: 0 868 2169 Size: 868 1301 BsaHI Gr'CG_yC Cuts at: 0 636 983 2169 Size: 636 347 1186 Fragments arranged by size: 1186 636 347 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 679 709 973 1003 1914 1944 2169 Size: 679 30 264 30 911 30 225 Fragments arranged by size: 911 679 264 225 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 330 684 1485 1599 1919 2169 Size: 330 354 801 114 320 250 Fragments arranged by size: 801 354 330 320 250 114 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 93 147 222 445 465 613 912 1390 Size: 93 54 75 223 20 148 299 478 Cuts at: 1390 1981 2169 Size: 591 188 Fragments arranged by size: 591 478 299 223 188 148 93 75 54 20 BsiEI CG_ry'CG Cuts at: 0 576 2169 Size: 576 1593 BsiHKAI G_wGCw'C Cuts at: 0 665 1466 1532 1955 2169 Size: 665 801 66 423 214 Fragments arranged by size: 801 665 423 214 66 BsmI GAATG_Cn' Cuts at: 0 1154 1321 2169 Size: 1154 167 848 Fragments arranged by size: 1154 848 167 BsmBI CGTCTCn'nnnn_ Cuts at: 0 643 2169 Size: 643 1526 Bsp1286I G_dGCh'C Cuts at: 0 181 665 704 1427 1466 1481 1532 1677 Size: 181 484 39 723 39 15 51 145 Cuts at: 1677 1955 2169 Size: 278 214 Fragments arranged by size: 723 484 278 214 181 145 51 39 39 15 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 121 635 766 2169 Size: 121 514 131 1403 Fragments arranged by size: 1403 514 131 121 BsrBI CCG'CTC Cuts at: 0 102 141 578 692 2169 Size: 102 39 437 114 1477 Fragments arranged by size: 1477 437 114 102 39 BsrDI GCAATG_nn' Cuts at: 0 915 2071 2169 Size: 915 1156 98 Fragments arranged by size: 1156 915 98 BsrFI r'CCGG_y Cuts at: 0 449 2169 Size: 449 1720 BsrGI T'GTAC_A Cuts at: 0 34 1749 2169 Size: 34 1715 420 Fragments arranged by size: 1715 420 34 BstAPI GCAn_nnn'nTGC Cuts at: 0 588 758 782 935 2169 Size: 588 170 24 153 1234 Fragments arranged by size: 1234 588 170 153 24 BstEII G'GTnAC_C Cuts at: 0 534 2169 Size: 534 1635 BstXI CCAn_nnnn'nTGG Cuts at: 0 1344 2169 Size: 1344 825 BstYI r'GATC_y Cuts at: 0 863 1340 2169 Size: 863 477 829 Fragments arranged by size: 863 829 477 Bsu36I CC'TnA_GG Cuts at: 0 322 1497 2169 Size: 322 1175 672 Fragments arranged by size: 1175 672 322 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 720 1005 2169 Size: 720 285 1164 Fragments arranged by size: 1164 720 285 BtsI GCAGTG_nn' Cuts at: 0 159 582 2169 Size: 159 423 1587 Fragments arranged by size: 1587 423 159 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 415 450 2169 Size: 415 35 1719 Fragments arranged by size: 1719 415 35 DraI TTT'AAA Cuts at: 0 1095 2031 2169 Size: 1095 936 138 Fragments arranged by size: 1095 936 138 DraIII CAC_nnn'GTG Cuts at: 0 420 2169 Size: 420 1749 EaeI y'GGCC_r Cuts at: 0 809 957 1311 2169 Size: 809 148 354 858 Fragments arranged by size: 858 809 354 148 EarI CTCTTCn'nnn_ Cuts at: 0 1015 1471 2169 Size: 1015 456 698 Fragments arranged by size: 1015 698 456 EcoICRI GAG'CTC Cuts at: 0 1464 1953 2169 Size: 1464 489 216 Fragments arranged by size: 1464 489 216 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 996 1144 1321 1608 1678 1743 1751 2011 Size: 996 148 177 287 70 65 8 260 Cuts at: 2011 2140 2155 2169 Size: 129 15 14 Fragments arranged by size: 996 287 260 177 148 129 70 65 15 14 8 EcoNI CCTnn'n_nnAGG Cuts at: 0 712 2169 Size: 712 1457 EcoO109I rG'GnC_Cy Cuts at: 0 1477 2127 2169 Size: 1477 650 42 Fragments arranged by size: 1477 650 42 EcoRI G'AATT_C Cuts at: 0 1069 1906 2169 Size: 1069 837 263 Fragments arranged by size: 1069 837 263 EcoRV GAT'ATC Cuts at: 0 2076 2169 Size: 2076 93 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 105 699 2169 Size: 105 594 1470 Fragments arranged by size: 1470 594 105 FspI TGC'GCA Cuts at: 0 358 1150 2169 Size: 358 792 1019 Fragments arranged by size: 1019 792 358 HaeII r_GCGC'y Cuts at: 0 651 684 750 845 1019 2169 Size: 651 33 66 95 174 1150 Fragments arranged by size: 1150 651 174 95 66 33 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 36 68 679 711 973 1005 1182 1214 Size: 36 32 611 32 262 32 177 32 Cuts at: 1214 1257 1289 1729 1761 1876 1908 2169 Size: 43 32 440 32 115 32 261 Fragments arranged by size: 611 440 262 261 177 115 43 36 32 32 32 32 32 32 32 MfeI C'AATT_G Cuts at: 0 432 1817 2169 Size: 432 1385 352 Fragments arranged by size: 1385 432 352 MscI TGG'CCA Cuts at: 0 811 959 1313 2169 Size: 811 148 354 856 Fragments arranged by size: 856 811 354 148 MslI CAynn'nnrTG Cuts at: 0 803 1255 2169 Size: 803 452 914 Fragments arranged by size: 914 803 452 MspA1I CmG'CkG Cuts at: 0 281 516 612 2169 Size: 281 235 96 1557 Fragments arranged by size: 1557 281 235 96 NaeI GCC'GGC Cuts at: 0 451 2169 Size: 451 1718 NgoMIV G'CCGG_C Cuts at: 0 449 2169 Size: 449 1720 NspI r_CATG'y Cuts at: 0 591 657 1874 2169 Size: 591 66 1217 295 Fragments arranged by size: 1217 591 295 66 PciI A'CATG_T Cuts at: 0 1870 2169 Size: 1870 299 PflMI CCAn_nnn'nTGG Cuts at: 0 1836 2169 Size: 1836 333 PspOMI G'GGCC_C Cuts at: 0 700 1477 2169 Size: 700 777 692 Fragments arranged by size: 777 700 692 PstI C_TGCA'G Cuts at: 0 521 1331 2169 Size: 521 810 838 Fragments arranged by size: 838 810 521 PvuII CAG'CTG Cuts at: 0 516 612 2169 Size: 516 96 1557 Fragments arranged by size: 1557 516 96 SacI G_AGCT'C Cuts at: 0 1466 1955 2169 Size: 1466 489 214 Fragments arranged by size: 1466 489 214 SapI GCTCTTCn'nnn_ Cuts at: 0 1471 2169 Size: 1471 698 ScaI AGT'ACT Cuts at: 0 901 2169 Size: 901 1268 SexAI A'CCwGG_T Cuts at: 0 416 2169 Size: 416 1753 SfcI C'TryA_G Cuts at: 0 517 594 1327 1380 2169 Size: 517 77 733 53 789 Fragments arranged by size: 789 733 517 77 53 SmlI C'TyrA_G Cuts at: 0 673 787 1141 2169 Size: 673 114 354 1028 Fragments arranged by size: 1028 673 354 114 SphI G_CATG'C Cuts at: 0 591 2169 Size: 591 1578 StuI AGG'CCT Cuts at: 0 793 1386 1663 2169 Size: 793 593 277 506 Fragments arranged by size: 793 593 506 277 StyI C'CwwG_G Cuts at: 0 1289 1427 1646 1856 2169 Size: 1289 138 219 210 313 Fragments arranged by size: 1289 313 219 210 138 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 893 1585 2169 Size: 893 692 584 Fragments arranged by size: 893 692 584 TatI w'GTAC_w Cuts at: 0 34 899 1749 2169 Size: 34 865 850 420 Fragments arranged by size: 865 850 420 34 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 865 897 2169 Size: 865 32 1272 Fragments arranged by size: 1272 865 32 Tth111I GACn'n_nGTC Cuts at: 0 823 2169 Size: 823 1346 XbaI T'CTAG_A Cuts at: 0 1200 2169 Size: 1200 969 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 35 414 1344 1653 2169 Size: 35 379 930 309 516 Fragments arranged by size: 930 516 379 309 35 XmnI GAAnn'nnTTC Cuts at: 0 547 2164 2169 Size: 547 1617 5 Fragments arranged by size: 1617 547 5 Enzymes that do cut: AarI AcuI AfeI AflIII AhdI AlfI AlwNI ApaI ApaLI ApoI AvaI BaeI BanII BbsI BglII Bme1580I BmgBI BmrI BpmI BpuEI BsaAI BsaBI BsaHI BsaXI BseRI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgZI BtsI CspCI DraI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I MfeI MscI MslI MspA1I NaeI NgoMIV NspI PciI PflMI PspOMI PstI PvuII SacI SapI ScaI SexAI SfcI SmlI SphI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XmnI Enzymes that do not cut: AatII AccI Acc65I AclI AflII AgeI AleI AloI AscI AseI AsiSI AvrII BamHI BanI BbeI BbvCI BcgI BciVI BclI BfrBI BglI BlpI BmtI BplI Bpu10I BsaI BsaWI BseYI BsiWI BspEI BspHI BssHII BssSI BstBI BstZ17I BtgI ClaI DrdI EagI EciI FseI FspAI HincII HindIII HpaI KasI KpnI MluI MmeI NarI NcoI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI SwaI XhoI XmaI ZraI