################################## # NO CUT ENZYMES ( 159 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AvrII BamHI BanI BbeI BbrPI BbvCI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstYI BstZ17I CelII Cfr10I ClaI CpoI DpnII DraI DraIII DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV FbaI FseI HapII HgaI Hin1I HincII HindII HpaI HpaII KasI KpnI KspI MamI MboI MfeI MflI MluI MroI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI PstI PvuI RcaI RsrII SacII SalI SapI Sau3AI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI Tth111I XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 38 enzymes) # ################################### AclI 337 AACGTT AA^CGTT AspHI 10 GAGCTC G[AT]GC[AT]^C AviII 549 TGCGCA TGC^GCA BalI 464 TGGCCA TGG^CCA BanII 10 GAGCTC G[GA]GC[CT]^C BcgI 552 GCAGGCTTCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 825 GGATAC GTATCC(6/5) BmyI 10 GAGCTC G[AGT]GC[ACT]^C BpmI 13 CTCCAG CTGGAG(16/14) BseRI 532 GAGGAG GAGGAG(10/8) BsiHKAI 10 GAGCTC G[AT]GC[AT]^C BsmI 616 GAATGC GAATGC(1/-1) Bsp1286I 10 GAGCTC G[AGT]GC[ACT]^C BstXI 141 CCACAGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 499 GACACCGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 464 TGGCCA [CT]^GGCC[GA] EcoT14I 666 CCAAGG C^C[AT][AT]GG FspI 549 TGCGCA TGC^GCA HaeII 806 GGCGCT [GA]GCGC^[CT] HindIII 837 AAGCTT A^AGCTT MluNI 464 TGGCCA TGG^CCA MscI 464 TGGCCA TGG^CCA MseI 971 TTAA T^TAA MspA1I 81 CAGCTG C[AC]G^C[GT]G PleI 214 GAGTC GAGTC(4/5) PpuMI 962 GGGACCT [GA]G^G[AT]CC[CT] Psp1406I 337 AACGTT AA^CGTT PvuII 81 CAGCTG CAG^CTG SacI 10 GAGCTC GAGCT^C SspI 579 AATATT AAT^ATT StyI 666 CCAAGG C^C[AT][AT]GG TaqI 795 TCGA T^CGA TfiI 274 GATTC G^A[AT]TC Tru9I 971 TTAA T^TAA Tsp509I 646 AATT ^AATT TthHB8I 795 TCGA T^CGA XbaI 162 TCTAGA T^CTAGA XcmI 385 CCATTGTTTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AfaI 399 GTAC GT^AC 564 GTAC AlwNI 118 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG 989 CAGTGACTG AocI 529 CCTGAGG CC^T[ACGT]AGG 738 CCTCAGG AvaII 963 GGACC G^G[AT]CC 1006 GGTCC BbsI 376 GAAGAC GAAGAC(2/6) 715 GAAGAC BfaI 163 CTAG C^TAG 569 CTAG BpuAI 376 GAAGAC GAAGAC(2/6) 715 GAAGAC BsaI 166 GAGACC GGTCTC(1/5) 198 GGTCTC Bsu36I 529 CCTGAGG CC^T[ACGT]AGG 738 CCTCAGG DraII 669 AGGCCCT [GA]G^G[ACGT]CC[CT] 962 GGGACCT EarI 88 CTCTTC CTCTTC(1/4) 346 CTCTTC Eco81I 529 CCTGAGG CC^T[ACGT]AGG 738 CCTCAGG EcoO109I 669 AGGCCCT [GA]G^G[ACGT]CC[CT] 962 GGGACCT EcoT22I 452 ATGCAT ATGCA^T 618 ATGCAT FauI 458 CCCGC CCCGC(4/6) 891 GCGGG HinfI 214 GAGTC G^A[ACGT]TC 274 GATTC Ksp632I 88 CTCTTC CTCTTC(1/4) 346 CTCTTC MaeI 163 CTAG C^TAG 569 CTAG MaeII 147 ACGT A^CGT 338 ACGT NsiI 452 ATGCAT ATGCA^T 618 ATGCAT PflMI 117 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 467 CCATCATCTGG RsaI 399 GTAC GT^AC 564 GTAC Tsp45I 31 GTCAC ^GT[GC]AC 991 GTGAC Van91I 117 CCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 467 CCATCATCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 37 enzymes) # ######################################### AciI AluI BbvI BsaJI BsiYI BslI BsmAI BsmFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HhaI HinP1I HphI ItaI MaeIII MboII MnlI MslI MvaI MwoI NlaIII NlaIV Sau96I ScrFI SfaNI TseI TspRI