################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AccIII AciI AclI AcsI AcyI AflII AgeI AhdI Aor51HI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BsmFI BsmI BsoBI BspDI BspEI BspHI BspMI BsrBI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HgaI Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MfeI MluI MroI MunI NarI NciI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI SbfI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccII 174 CGCG CG^CG AflIII 534 ACATGT A^C[GA][CT]GT Alw44I 568 GTGCAC G^TGCAC ApaI 478 GGGCCC GGGCC^C ApaLI 568 GTGCAC G^TGCAC BalI 95 TGGCCA TGG^CCA BamHI 248 GGATCC G^GATCC BanI 523 GGTGCC G^G[CT][GA]CC BbvCI 362 CCTCAGC CCTCAGC(-5/-2) BsaBI 426 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsmAI 900 GTCTC GTCTC(1/5) Bsp1407I 230 TGTACA T^GTACA BspLU11I 534 ACATGT A^CATGT BsrFI 773 GCCGGC [GA]^CCGG[CT] BsrGI 230 TGTACA T^GTACA BssSI 234 CACGAG CACGAG(-5/-1) BstAPI 257 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 174 CGCG CG^CG BstXI 689 CCATTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 773 GCCGGC [GA]^CCGG[CT] EcoNI 503 CCTACTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 581 CCATGG C^C[AT][AT]GG HaeII 646 AGCGCC [GA]GCGC^[CT] HapII 774 CCGG C^CGG HinfI 69 GATTC G^A[ACGT]TC HpaII 774 CCGG C^CGG MaeIII 295 GTCAC ^GT[ACGT]AC MamI 426 GATGGAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MboII 25 GAAGA GAAGA(8/7) MluNI 95 TGGCCA TGG^CCA MscI 95 TGGCCA TGG^CCA MslI 888 CACGGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 774 CCGG C^CGG MvnI 174 CGCG CG^CG NaeI 773 GCCGGC GCC^GGC NcoI 581 CCATGG C^CATGG NgoMIV 773 GCCGGC G^CCGGC NspI 534 ACATGT [GA]CATG^[CT] PflMI 254 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PstI 858 CTGCAG CTGCA^G ScaI 785 AGTACT AGT^ACT SexAI 452 ACCTGGT A^CC[AT]GGT SfcI 858 CTGCAG C^T[GA][CT]AG SspBI 230 TGTACA T^GTACA StyI 581 CCATGG C^C[AT][AT]GG TaqI 203 TCGA T^CGA TfiI 69 GATTC G^A[AT]TC Tsp45I 295 GTCAC ^GT[GC]AC Tsp509I 835 AATT ^AATT TthHB8I 203 TCGA T^CGA Van91I 254 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 21 TCTAGA T^CTAGA XcmI 751 CCACTGTTCGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AocI 904 CCTGAGG CC^T[ACGT]AGG 915 CCTCAGG AspHI 264 GTGCTC G[AT]GC[AT]^C 568 GTGCAC BfaI 22 CTAG C^TAG 574 CTAG BsiHKAI 264 GTGCTC G[AT]GC[AT]^C 568 GTGCAC BstYI 248 GGATCC [GA]^GATC[CT] 926 GGATCT Bsu36I 904 CCTGAGG CC^T[ACGT]AGG 915 CCTCAGG Cac8I 105 GCCTGC GC[ACGT]^[ACGT]GC 773 GCCGGC CfoI 175 GCGC GCG^C 647 GCGC EaeI 95 TGGCCA [CT]^GGCC[GA] 311 CGGCCA Eco81I 904 CCTGAGG CC^T[ACGT]AGG 915 CCTCAGG HhaI 175 GCGC GCG^C 647 GCGC HinP1I 175 GCGC G^CGC 647 GCGC MaeI 22 CTAG C^TAG 574 CTAG MflI 248 GGATCC [GA]^GATC[CT] 926 GGATCT MseI 392 TTAA T^TAA 850 TTAA MspA1I 258 CAGCTG C[AC]G^C[GT]G 855 CAGCTG PpuMI 279 AGGTCCT [GA]G^G[AT]CC[CT] 470 AGGACCT PvuII 258 CAGCTG CAG^CTG 855 CAGCTG Tru9I 392 TTAA T^TAA 850 TTAA XhoII 248 GGATCC [GA]^GATC[CT] 926 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 44 enzymes) # ######################################### AfaI AluI AlwI AlwNI AvaII BanII BbvI BmyI BpmI BsaJI BseRI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI Cfr13I DdeI DpnII DraII DsaI EcoO109I EcoRII Fnu4HI FokI HaeIII HphI ItaI MboI MnlI MvaI MwoI NdeII NlaIII NlaIV RsaI Sau3AI Sau96I ScrFI SfaNI TseI TspRI