(Linear) (Six-base) MAPSORT of: KIEE6612.seq Check: 36 from: 1 to: 933 KIEE6612.orf With 182 enzymes: SgfI * March 16, 2015 11:34 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 296 400 636 933 Size: 296 104 236 297 Fragments arranged by size: 297 296 236 104 AflIII A'CryG_T Cuts at: 0 534 933 Size: 534 399 AleI CACnn'nnGTG Cuts at: 0 892 933 Size: 892 41 AlwNI CAG_nnn'CTG Cuts at: 0 260 595 923 933 Size: 260 335 328 10 Fragments arranged by size: 335 328 260 10 ApaI G_GGCC'C Cuts at: 0 482 933 Size: 482 451 ApaLI G'TGCA_C Cuts at: 0 568 933 Size: 568 365 BamHI G'GATC_C Cuts at: 0 248 933 Size: 248 685 BanI G'GyrC_C Cuts at: 0 523 933 Size: 523 410 BanII G_rGCy'C Cuts at: 0 166 355 482 933 Size: 166 189 127 451 Fragments arranged by size: 451 189 166 127 BbvCI CC'TCA_GC Cuts at: 0 363 933 Size: 363 570 Bme1580I G_kGCm'C Cuts at: 0 482 572 933 Size: 482 90 361 Fragments arranged by size: 482 361 90 BmrI ACTGGGnnnn_n' Cuts at: 0 160 477 933 Size: 160 317 456 Fragments arranged by size: 456 317 160 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 200 390 487 528 766 933 Size: 200 190 97 41 238 167 Fragments arranged by size: 238 200 190 167 97 41 Bpu10I CC'TnA_GC Cuts at: 0 363 933 Size: 363 570 BsaBI GATnn'nnATC Cuts at: 0 430 933 Size: 430 503 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 173 203 543 573 933 Size: 173 30 340 30 360 Fragments arranged by size: 360 340 173 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 525 564 883 904 933 Size: 525 39 319 21 29 Fragments arranged by size: 525 319 39 29 21 BseYI C'CCAG_C Cuts at: 0 253 933 Size: 253 680 BsiHKAI G_wGCw'C Cuts at: 0 268 572 933 Size: 268 304 361 Fragments arranged by size: 361 304 268 Bsp1286I G_dGCh'C Cuts at: 0 166 268 355 482 572 933 Size: 166 102 87 127 90 361 Fragments arranged by size: 361 166 127 102 90 87 BsrFI r'CCGG_y Cuts at: 0 773 933 Size: 773 160 BsrGI T'GTAC_A Cuts at: 0 230 933 Size: 230 703 BssSI C'ACGA_G Cuts at: 0 234 933 Size: 234 699 BstAPI GCAn_nnn'nTGC Cuts at: 0 263 933 Size: 263 670 BstXI CCAn_nnnn'nTGG Cuts at: 0 696 933 Size: 696 237 BstYI r'GATC_y Cuts at: 0 248 926 933 Size: 248 678 7 Fragments arranged by size: 678 248 7 Bsu36I CC'TnA_GG Cuts at: 0 905 916 933 Size: 905 11 17 Fragments arranged by size: 905 17 11 BtgI C'CryG_G Cuts at: 0 581 800 887 933 Size: 581 219 87 46 Fragments arranged by size: 581 219 87 46 EaeI y'GGCC_r Cuts at: 0 95 311 933 Size: 95 216 622 Fragments arranged by size: 622 216 95 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 200 296 390 400 487 528 636 766 Size: 200 96 94 10 87 41 108 130 Cuts at: 766 933 Size: 167 Fragments arranged by size: 200 167 130 108 96 94 87 41 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 507 933 Size: 507 426 EcoO109I rG'GnC_Cy Cuts at: 0 280 471 478 479 920 933 Size: 280 191 7 1 441 13 Fragments arranged by size: 441 280 191 13 7 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 168 405 933 Size: 168 237 528 Fragments arranged by size: 528 237 168 HaeII r_GCGC'y Cuts at: 0 650 933 Size: 650 283 MscI TGG'CCA Cuts at: 0 97 933 Size: 97 836 MslI CAynn'nnrTG Cuts at: 0 892 933 Size: 892 41 MspA1I CmG'CkG Cuts at: 0 260 857 933 Size: 260 597 76 Fragments arranged by size: 597 260 76 NaeI GCC'GGC Cuts at: 0 775 933 Size: 775 158 NcoI C'CATG_G Cuts at: 0 581 933 Size: 581 352 NgoMIV G'CCGG_C Cuts at: 0 773 933 Size: 773 160 NspI r_CATG'y Cuts at: 0 538 933 Size: 538 395 PciI A'CATG_T Cuts at: 0 534 933 Size: 534 399 PflMI CCAn_nnn'nTGG Cuts at: 0 260 933 Size: 260 673 PpuMI rG'GwC_Cy Cuts at: 0 280 471 933 Size: 280 191 462 Fragments arranged by size: 462 280 191 PspOMI G'GGCC_C Cuts at: 0 478 933 Size: 478 455 PstI C_TGCA'G Cuts at: 0 862 933 Size: 862 71 PvuII CAG'CTG Cuts at: 0 260 857 933 Size: 260 597 76 Fragments arranged by size: 597 260 76 ScaI AGT'ACT Cuts at: 0 787 933 Size: 787 146 SexAI A'CCwGG_T Cuts at: 0 452 933 Size: 452 481 SfcI C'TryA_G Cuts at: 0 858 933 Size: 858 75 StyI C'CwwG_G Cuts at: 0 581 933 Size: 581 352 TatI w'GTAC_w Cuts at: 0 126 191 230 785 933 Size: 126 65 39 555 148 Fragments arranged by size: 555 148 126 65 39 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 173 205 264 296 933 Size: 173 32 59 32 637 Fragments arranged by size: 637 173 59 32 32 XbaI T'CTAG_A Cuts at: 0 21 933 Size: 21 912 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 758 933 Size: 758 175 Enzymes that do cut: AcuI AflIII AleI AlwNI ApaI ApaLI BamHI BanI BanII BbvCI Bme1580I BmrI BpmI Bpu10I BsaBI BsaXI BseRI BseYI BsiHKAI Bsp1286I BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI EaeI Eco57MI EcoNI EcoO109I FalI HaeII MscI MslI MspA1I NaeI NcoI NgoMIV NspI PciI PflMI PpuMI PspOMI PstI PvuII ScaI SexAI SfcI StyI TatI TstI XbaI XcmI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AgeI AhdI AlfI AloI ApoI AscI AseI AsiSI AvaI AvrII BaeI BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI BmgBI BmtI BplI BpuEI BsaI BsaAI BsaHI BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BssHII BstBI BstEII BstZ17I BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EarI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI NarI NdeI NheI NotI NruI NsiI PacI PasI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI SbfI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII Tth111I XhoI XmaI XmnI ZraI