Restriction map for
pFN21AE5423
(Circular) (Six-base) MAPSORT of: pFN21AE5423.seq Check: 4064 from: 1 to: 5749
pFN21AE5423 5749 bp
With 182 enzymes: SgfI *
January 30, 2014 17:19 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3559 278
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2053 3092 1937
Size: 116 1039 4594
Fragments arranged by size:
4594 1039 116
Acc65I G'GTAC_C
Cuts at: 3070 3070
Size: 5749
AclI AA'CG_TT
Cuts at: 1563 3876 4249 1563
Size: 2313 373 3063
Fragments arranged by size:
3063 2313 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2187 2354 3812 5196 5464 1632
Size: 555 167 1458 1384 268 1917
Fragments arranged by size:
1917 1458 1384 555 268 167
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5552
AflIII A'CryG_T
Cuts at: 1203 3012 4648 5625 1203
Size: 1809 1636 977 1327
Fragments arranged by size:
1809 1636 1327 977
AgeI A'CCGG_T
Cuts at: 4580 4580
Size: 5749
AhdI GACnn_n'nnGTC
Cuts at: 4478 4478
Size: 5749
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5749
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5749
AlwNI CAG_nnn'CTG
Cuts at: 2047 2757 3492 5064 2047
Size: 710 735 1572 2732
Fragments arranged by size:
2732 1572 735 710
ApaI G_GGCC'C
Cuts at: 2087 2087
Size: 5749
ApaLI G'TGCA_C
Cuts at: 3806 4962 3806
Size: 1156 4593
ApoI r'AATT_y
Cuts at: 1493 2492 3058 3284 3436 5545 1493
Size: 999 566 226 152 2109 1697
Fragments arranged by size:
2109 1697 999 566 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4303 160
Size: 872 3271 1606
Fragments arranged by size:
3271 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
AvaI C'yCGr_G
Cuts at: 1870 1943 2057 2086 3074 1870
Size: 73 114 29 988 4545
Fragments arranged by size:
4545 988 114 73 29
BamHI G'GATC_C
Cuts at: 3079 3446 3079
Size: 367 5382
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2036 3070 4525 618
Size: 325 217 627 249 1034 1455 1842
Fragments arranged by size:
1842 1455 1034 627 325 249 217
BanII G_rGCy'C
Cuts at: 1391 2087 3068 1391
Size: 696 981 4072
Fragments arranged by size:
4072 981 696
BbeI G_GCGC'C
Cuts at: 2040 2040
Size: 5749
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2117 928
Size: 971 218 4560
Fragments arranged by size:
4560 971 218
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 3940 3974 1903
Size: 34 50 34 1919 34 3678
Fragments arranged by size:
3678 1919 50 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3641 4851 3641
Size: 1210 4539
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5749
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4360 136
Size: 107 122 71 631 605 2688 1525
Fragments arranged by size:
2688 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5744 5744
Size: 5749
BlpI GC'TnA_GC
Cuts at: 3166 3166
Size: 5749
Bme1580I G_kGCm'C
Cuts at: 1790 2087 3810 4966 1790
Size: 297 1723 1156 2573
Fragments arranged by size:
2573 1723 1156 297
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5749
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3480 4438 442
Size: 416 53 83 399 24 2063 958 1753
Fragments arranged by size:
2063 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5749
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2745 4409 1239
Size: 1506 1664 2579
Fragments arranged by size:
2579 1664 1506
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3874 4739 5037 3874
Size: 865 298 4586
Fragments arranged by size:
4586 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2203 2713 4412 882
Size: 617 704 510 1699 2219
Fragments arranged by size:
2219 1699 704 617 510
BsaAI yAC'GTr
Cuts at: 493 1204 3540 493
Size: 711 2336 2702
Fragments arranged by size:
2702 2336 711
BsaBI GATnn'nnATC
Cuts at: 2157 5743 2157
Size: 3586 2163
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2037 3556 3938 4573
Size: 53 83 186 936 504 1519 382 635
Cuts at: 4573 275
Size: 1451
Fragments arranged by size:
1519 1451 936 635 504 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4182 4580 4854 5001 5412 4182
Size: 398 274 147 411 4519
Fragments arranged by size:
4519 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5524
Fragments arranged by size:
5524 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2568 1186
Size: 1382 4367
BseYI C'CCAG_C
Cuts at: 2047 2314 4952 5448 2047
Size: 267 2638 496 2348
Fragments arranged by size:
2638 2348 496 267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 5176
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3199 3960 4988 1540
Size: 275 186 1198 761 1028 2301
Fragments arranged by size:
2301 1198 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3810 3895 4966 3810
Size: 85 1071 4593
Fragments arranged by size:
4593 1071 85
BsmI GAATG_Cn'
Cuts at: 3260 3353 3260
Size: 93 5656
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2087 3068 3810 3895 4966 1391
Size: 399 297 981 742 85 1071 2174
Fragments arranged by size:
2174 1071 981 742 399 297 85
BspEI T'CCGG_A
Cuts at: 5412 5412
Size: 5749
BspHI T'CATG_A
Cuts at: 2658 3638 5368 2658
Size: 980 1730 3039
Fragments arranged by size:
3039 1730 980
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2020 3104 844
Size: 1176 1084 3489
Fragments arranged by size:
3489 1176 1084
BsrBI CCG'CTC
Cuts at: 1394 1823 3636 1394
Size: 429 1813 3507
Fragments arranged by size:
3507 1813 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4244 4418 65
Size: 1183 2996 174 1396
Fragments arranged by size:
2996 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4393 4580 1700
Size: 110 2583 187 2869
Fragments arranged by size:
2869 2583 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5749
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5749
BssSI C'ACGA_G
Cuts at: 3809 4821 3809
Size: 1012 4737
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5749
BstBI TT'CG_AA
Cuts at: 4553 4553
Size: 5749
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5749
BstYI r'GATC_y
Cuts at: 1972 2929 3079 3446 3831 3848 5300 5386 5398
Size: 957 150 367 385 17 1452 86 12
Cuts at: 5398 5744 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 957 385 367 346 150 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2255 513
Size: 552 1190 4007
Fragments arranged by size:
4007 1190 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5010
Fragments arranged by size:
5010 683 56
BtsI GCAGTG_nn'
Cuts at: 3265 4059 4079 3265
Size: 794 20 4935
Fragments arranged by size:
4935 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5749
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5714
DraI TTT'AAA
Cuts at: 2677 3053 2677
Size: 376 5373
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2387 4756 809
Size: 507 1071 2369 1802
Fragments arranged by size:
2369 1802 1071 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3196 4085 5714
Size: 54 1425 50 275 18 1366 889 1629
Cuts at: 5714 8
Size: 43
Fragments arranged by size:
1629 1425 1366 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3196 1537
Size: 275 1384 4090
Fragments arranged by size:
4090 1384 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2973 3679 1353
Size: 1620 706 3423
Fragments arranged by size:
3423 1620 706
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4336 4710 4856 387
Size: 1064 138 2747 374 146 1280
Fragments arranged by size:
2747 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2187 2354 2745 3812 4409 5196 5464
Size: 393 555 167 391 1067 597 787 268
Cuts at: 5464 1239
Size: 1524
Fragments arranged by size:
1524 1067 787 597 555 393 391 268
167
EcoRI G'AATT_C
Cuts at: 3058 3436 3058
Size: 378 5371
FspI TGC'GCA
Cuts at: 4255 4255
Size: 5749
HaeII r_GCGC'y
Cuts at: 1678 2040 4896 1678
Size: 362 2856 2531
Fragments arranged by size:
2856 2531 362
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4390 4422 4464 4496 1373
Size: 32 135 32 2818 32 42 32 2626
Fragments arranged by size:
2818 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2054 3093 3339 669
Size: 988 281 116 1039 246 3079
Fragments arranged by size:
3079 1039 988 281 246 116
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5749
HpaI GTT'AAC
Cuts at: 3339 3339
Size: 5749
KasI G'GCGC_C
Cuts at: 2036 2036
Size: 5749
KpnI G_GTAC'C
Cuts at: 3074 3074
Size: 5749
MfeI C'AATT_G
Cuts at: 3348 3348
Size: 5749
MluI A'CGCG_T
Cuts at: 5625 5625
Size: 5749
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 1994 4863 5047 1305
Size: 689 2869 184 2007
Fragments arranged by size:
2869 2007 689 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3927
Fragments arranged by size:
3927 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3707 4066 4225 518
Size: 648 2541 359 159 2042
Fragments arranged by size:
2541 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3165 3842 4990 5235 1606
Size: 1559 677 1148 245 2120
Fragments arranged by size:
2120 1559 1148 677 245
NarI GG'CG_CC
Cuts at: 2037 2037
Size: 5749
NcoI C'CATG_G
Cuts at: 513 1065 2255 513
Size: 552 1190 4007
Fragments arranged by size:
4007 1190 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5749
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5749
NotI GC'GGCC_GC
Cuts at: 3196 3196
Size: 5749
NspI r_CATG'y
Cuts at: 1132 1229 2357 3107 4652 1132
Size: 97 1128 750 1545 2229
Fragments arranged by size:
2229 1545 1128 750 97
PasI CC'CwG_GG
Cuts at: 1348 2080 1348
Size: 732 5017
PciI A'CATG_T
Cuts at: 4648 4648
Size: 5749
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2047 1108
Size: 939 4810
PfoI T'CCnGG_A
Cuts at: 2602 2602
Size: 5749
PmeI GTTT'AAAC
Cuts at: 3053 3053
Size: 5749
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3818 3850 5331 5363 3818
Size: 32 1481 32 4204
Fragments arranged by size:
4204 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2030 1613
Size: 417 5332
PsiI TTA'TAA
Cuts at: 3319 3319
Size: 5749
PspOMI G'GGCC_C
Cuts at: 2083 2083
Size: 5749
PstI C_TGCA'G
Cuts at: 830 3101 830
Size: 2271 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5749
SalI G'TCGA_C
Cuts at: 1936 2052 3091 1936
Size: 116 1039 4594
Fragments arranged by size:
4594 1039 116
SbfI CC_TGCA'GG
Cuts at: 3101 3101
Size: 5749
ScaI AGT'ACT
Cuts at: 3997 5709 3997
Size: 1712 4037
SexAI A'CCwGG_T
Cuts at: 2278 2278
Size: 5749
SfcI C'TryA_G
Cuts at: 826 1045 2442 3097 4232 4913 5104 826
Size: 219 1397 655 1135 681 191 1471
Fragments arranged by size:
1471 1397 1135 681 655 219 191
SfoI GGC'GCC
Cuts at: 2038 2038
Size: 5749
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5749
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5749
SmaI CCC'GGG
Cuts at: 1872 2088 3076 1872
Size: 216 988 4545
Fragments arranged by size:
4545 988 216
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3853 4754 5016 820
Size: 197 926 1910 901 262 1553
Fragments arranged by size:
1910 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3540 493
Size: 3047 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5749
SphI G_CATG'C
Cuts at: 1132 3107 1132
Size: 1975 3774
SspI AAT'ATT
Cuts at: 5 52 3673 5
Size: 47 3621 2081
Fragments arranged by size:
3621 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5749
StyI C'CwwG_G
Cuts at: 513 1065 2255 2270 2337 3188 513
Size: 552 1190 15 67 851 3074
Fragments arranged by size:
3074 1190 851 552 67 15
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2060 3776 3793 3946 4131 4576 325
Size: 1255 480 1716 17 153 185 445 1498
Fragments arranged by size:
1716 1498 1255 480 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3995 5707 96
Size: 274 80 33 51 1443 2018 1712 138
Fragments arranged by size:
2018 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2598 2630 2805 2837 5709 5741 2598
Size: 32 175 32 2872 32 2606
Fragments arranged by size:
2872 2606 175 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5749
XbaI T'CTAG_A
Cuts at: 3085 3085
Size: 5749
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2720 2720
Size: 5749
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5749
XmaI C'CCGG_G
Cuts at: 1870 2086 3074 1870
Size: 216 988 4545
Fragments arranged by size:
4545 988 216
XmnI GAAnn'nnTTC
Cuts at: 3878 3878
Size: 5749
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3557 276
Size: 53 83 186 936 2023 2468
Fragments arranged by size:
2468 2023 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NspI PasI PciI
PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI SalI SbfI ScaI SexAI SfcI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI NaeI
NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SfiI SrfI
SwaI