Restriction map for pFN21AE5423
 (Linear) (Six-base) MAPSORT of: KIEE5423.seq  Check: 5223  from: 1  to: 1044



KIEE5423.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:59  ..

AccI GT'mk_AC

Cuts at:      0      48    1044 
   Size:         48     996
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     349    1044 
   Size:        182     167     695

  Fragments arranged by size: 

                 695     182     167
 
AflIII A'CryG_T

Cuts at:      0    1007    1044 
   Size:       1007      37
 
AlwNI CAG_nnn'CTG

Cuts at:      0      42     752    1044 
   Size:         42     710     292

  Fragments arranged by size: 

                 710     292      42
 
ApaI G_GGCC'C

Cuts at:      0      82    1044 
   Size:         82     962
 
ApoI r'AATT_y

Cuts at:      0     487    1044 
   Size:        487     557
 
AvaI C'yCGr_G

Cuts at:      0      52      81    1044 
   Size:         52      29     963

  Fragments arranged by size: 

                 963      52      29
 
BanI G'GyrC_C

Cuts at:      0      31    1044 
   Size:         31    1013
 
BanII G_rGCy'C

Cuts at:      0      82    1044 
   Size:         82     962
 
BbeI G_GCGC'C

Cuts at:      0      35    1044 
   Size:         35    1009
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     112    1044 
   Size:        112     932
 
Bme1580I G_kGCm'C

Cuts at:      0      82    1044 
   Size:         82     962
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     740    1044 
   Size:        740     304
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     198     708    1044 
   Size:        198     510     336

  Fragments arranged by size: 

                 510     336     198
 
BsaBI GATnn'nnATC

Cuts at:      0     152    1044 
   Size:        152     892
 
BsaHI Gr'CG_yC

Cuts at:      0      32    1044 
   Size:         32    1012
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     563    1044 
   Size:        563     481
 
BseYI C'CCAG_C

Cuts at:      0      42     309    1044 
   Size:         42     267     735

  Fragments arranged by size: 

                 735     267      42
 
Bsp1286I G_dGCh'C

Cuts at:      0      82    1044 
   Size:         82     962
 
BspHI T'CATG_A

Cuts at:      0     653    1044 
   Size:        653     391
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      15    1044 
   Size:         15    1029
 
BstYI r'GATC_y

Cuts at:      0     924    1044 
   Size:        924     120
 
BtgI C'CryG_G

Cuts at:      0     250    1044 
   Size:        250     794
 
DraI TTT'AAA

Cuts at:      0     672    1044 
   Size:        672     372
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     382    1044 
   Size:        382     662
 
EarI CTCTTCn'nnn_

Cuts at:      0     968    1044 
   Size:        968      76
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     182     349     740    1044 
   Size:        182     167     391     304

  Fragments arranged by size: 

                 391     304     182     167
 
HaeII r_GCGC'y

Cuts at:      0      35    1044 
   Size:         35    1009
 
HincII GTy'rAC

Cuts at:      0      49    1044 
   Size:         49     995
 
KasI G'GCGC_C

Cuts at:      0      31    1044 
   Size:         31    1013
 
NarI GG'CG_CC

Cuts at:      0      32    1044 
   Size:         32    1012
 
NcoI C'CATG_G

Cuts at:      0     250    1044 
   Size:        250     794
 
NspI r_CATG'y

Cuts at:      0     352    1044 
   Size:        352     692
 
PasI CC'CwG_GG

Cuts at:      0      75    1044 
   Size:         75     969
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      42    1044 
   Size:         42    1002
 
PfoI T'CCnGG_A

Cuts at:      0     597    1044 
   Size:        597     447
 
PshAI GACnn'nnGTC

Cuts at:      0      25    1044 
   Size:         25    1019
 
PspOMI G'GGCC_C

Cuts at:      0      78    1044 
   Size:         78     966
 
SalI G'TCGA_C

Cuts at:      0      47    1044 
   Size:         47     997
 
SexAI A'CCwGG_T

Cuts at:      0     273    1044 
   Size:        273     771
 
SfcI C'TryA_G

Cuts at:      0     437    1044 
   Size:        437     607
 
SfoI GGC'GCC

Cuts at:      0      33    1044 
   Size:         33    1011
 
SmaI CCC'GGG

Cuts at:      0      83    1044 
   Size:         83     961
 
StyI C'CwwG_G

Cuts at:      0     250     265     332    1044 
   Size:        250      15      67     712

  Fragments arranged by size: 

                 712     250      67      15
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0      55    1044 
   Size:         55     989
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     593     625     800     832    1044 
   Size:        593      32     175      32     212

  Fragments arranged by size: 

                 593     212     175      32      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     715    1044 
   Size:        715     329
 
XmaI C'CCGG_G

Cuts at:      0      81    1044 
   Size:         81     963
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApaI     ApoI     AvaI     BanI
    BanII     BbeI     BbsI Bme1580I     BpmI     BsaI    BsaBI    BsaHI
    BseRI    BseYI Bsp1286I    BspHI    BspMI    BstYI     BtgI     DraI
     DrdI     EarI  Eco57MI    HaeII   HincII     KasI     NarI     NcoI
     NspI     PasI    PflMI     PfoI    PshAI   PspOMI     SalI    SexAI
     SfcI     SfoI     SmaI     StyI    TaqII     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    ApaLI     AscI     AseI    AsiSI    AvrII
     BaeI    BamHI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI    BmgBI     BmrI     BmtI     BplI   Bpu10I    BpuEI
    BsaAI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI   DraIII     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    Hin4I
  HindIII     HpaI     KpnI     MfeI     MluI     MmeI     MscI     MslI
   MspA1I     NaeI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PciI     PmeI     PmlI     PpiI    PpuMI     PsiI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII    SanDI
     SapI     SbfI     ScaI     SfiI     SgfI    SgrAI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     SwaI     TatI  Tth111I
     XbaI     XhoI     XmnI     ZraI
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