################################## # NO CUT ENZYMES ( 120 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AgeI AhdI AocI Aor51HI ApaI AscI AseI AsnI AspEI AspI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BlnI BlpI Bpu1102I BsaAI BsaHI BsaWI BseAI BsgI BsiEI BsiWI BsmBI BspEI BspLU11I BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstZ17I Bsu36I CelII Cfr10I CpoI DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I FbaI FseI FspI HaeII HgaI Hin1I HpaI KasI Ksp632I KspI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I Van91I XbaI XhoI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 258 GGTACC G^GTACC AccII 2077 CGCG CG^CG AflIII 2184 ACGTGT A^C[GA][CT]GT Alw44I 120 GTGCAC G^TGCAC AlwI 2167 GATCC GGATC(4/5) AlwNI 571 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 120 GTGCAC G^TGCAC Asp700I 1532 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 258 GGTACC G^GTACC BbsI 1536 GTCTTC GAAGAC(2/6) BpmI 76 CTGGAG CTGGAG(16/14) BpuAI 1536 GTCTTC GAAGAC(2/6) BsaBI 343 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 291 GAGACC GGTCTC(1/5) BsmI 1250 GCATTC GAATGC(1/-1) BspDI 1312 ATCGAT AT^CGAT BspHI 1569 TCATGA T^CATGA BsrBI 28 CCGCTC CCGCTC(-3/-3) BstEII 853 GGTGACC G^GT[ACGT]ACC BstPI 853 GGTGACC G^GT[ACGT]ACC BstUI 2077 CGCG CG^CG BstXI 248 CCATTTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ClaI 1312 ATCGAT AT^CGAT DraI 736 TTTAAA TTT^AAA EcoO65I 853 GGTGACC G^GT[ACGT]ACC EcoRI 1738 GAATTC G^AATTC EcoRV 1185 GATATC GAT^ATC EcoT22I 346 ATGCAT ATGCA^T HincII 229 GTTGAC GT[CT]^[GA]AC HindII 229 GTTGAC GT[CT]^[GA]AC HindIII 1076 AAGCTT A^AGCTT KpnI 258 GGTACC GGTAC^C MaeII 2184 ACGT A^CGT MamI 343 GATATGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MvnI 2077 CGCG CG^CG NsiI 346 ATGCAT ATGCA^T PpuMI 1033 GGGACCC [GA]G^G[AT]CC[CT] RcaI 1569 TCATGA T^CATGA SacI 93 GAGCTC GAGCT^C SfcI 1177 CTACAG C^T[GA][CT]AG SmaI 150 CCCGGG CCC^GGG SphI 1293 GCATGC GCATG^C StuI 1646 AGGCCT AGG^CCT XcmI 1797 CCAGACTTCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 150 CCCGGG C^CCGGG XmaI 150 CCCGGG C^CCGGG XmnI 1532 GAATGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AvaII 1034 GGACC G^G[AT]CC 2143 GGACC BanII 93 GAGCTC G[GA]GC[CT]^C 1629 GGGCTC BfaI 479 CTAG C^TAG 884 CTAG BglII 398 AGATCT A^GATCT 1281 AGATCT Bsp1407I 44 TGTACA T^GTACA 1440 TGTACA BsrGI 44 TGTACA T^GTACA 1440 TGTACA BstF5I 1432 CATCC GGATG(2/0) 2129 CATCC BstYI 398 AGATCT [GA]^GATC[CT] 1281 AGATCT CfoI 15 GCGC GCG^C 1745 GCGC DraII 1033 GGGACCC [GA]G^G[ACGT]CC[CT] 2035 AGGGCCT DraIII 468 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG 1444 CACTTTGTG EcoNI 996 CCTTATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1494 CCTTGCAAAGG EcoO109I 1033 GGGACCC [GA]G^G[ACGT]CC[CT] 2035 AGGGCCT FokI 1432 CATCC GGATG(9/13) 2129 CATCC HhaI 15 GCGC GCG^C 1745 GCGC HinP1I 15 GCGC G^CGC 1745 GCGC MaeI 479 CTAG C^TAG 884 CTAG MflI 398 AGATCT [GA]^GATC[CT] 1281 AGATCT MseI 737 TTAA T^TAA 1021 TTAA SmlI 840 CTCAAG C^T[CT][GA]AG 1624 CTCAAG SspBI 44 TGTACA T^GTACA 1440 TGTACA Tru9I 737 TTAA T^TAA 1021 TTAA XhoII 398 AGATCT [GA]^GATC[CT] 1281 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI AspHI AvaI BanI BbvI BcnI BmyI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstNI Cac8I Cfr13I DdeI DpnII EcoRII EcoT14I FauI Fnu4HI HaeIII HapII HinfI HpaII HphI ItaI MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I