(Circular) (Six-base) MAPSORT of: pFN21AE3287.seq Check: 8729 from: 1 to: 6925 pFN21AE3287 6925 bp With 182 enzymes: SgfI * December 28, 2011 15:26 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2025 2025 Size: 6925 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4735 278 Size: 53 83 186 936 3199 2468 Fragments arranged by size: 3199 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4268 1937 Size: 2331 4594 Acc65I G'GTAC_C Cuts at: 2263 4246 2263 Size: 1983 4942 AclI AA'CG_TT Cuts at: 1563 5052 5425 1563 Size: 3489 373 3063 Fragments arranged by size: 3489 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2939 3099 3389 4988 6372 6640 1632 Size: 1307 160 290 1599 1384 268 1917 Fragments arranged by size: 1917 1599 1384 1307 290 268 160 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6728 AflIII A'CryG_T Cuts at: 1203 4189 5824 6801 1203 Size: 2986 1635 977 1327 Fragments arranged by size: 2986 1635 1327 977 AgeI A'CCGG_T Cuts at: 5756 5756 Size: 6925 AhdI GACnn_n'nnGTC Cuts at: 5654 5654 Size: 6925 AleI CACnn'nnGTG Cuts at: 1166 3902 1166 Size: 2736 4189 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3321 1184 Size: 2137 4788 AlwNI CAG_nnn'CTG Cuts at: 2581 4668 6240 2581 Size: 2087 1572 3266 Fragments arranged by size: 3266 2087 1572 ApaLI G'TGCA_C Cuts at: 2125 4982 6138 2125 Size: 2857 1156 2912 Fragments arranged by size: 2912 2857 1156 ApoI r'AATT_y Cuts at: 1493 2275 2304 2894 2963 3743 4234 4460 4612 Size: 782 29 590 69 780 491 226 152 Cuts at: 4612 6721 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 782 780 590 491 226 152 69 29 AseI AT'TA_AT Cuts at: 160 1032 5479 160 Size: 872 4447 1606 Fragments arranged by size: 4447 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6925 AvaI C'yCGr_G Cuts at: 1870 1943 2064 2110 2155 4250 1870 Size: 73 121 46 45 2095 4545 Fragments arranged by size: 4545 2095 121 73 46 45 BamHI G'GATC_C Cuts at: 4255 4622 4255 Size: 367 6558 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2159 2263 3896 4246 5701 Size: 325 217 627 372 104 1633 350 1455 Cuts at: 5701 618 Size: 1842 Fragments arranged by size: 1842 1633 1455 627 372 350 325 217 104 BanII G_rGCy'C Cuts at: 1391 2102 3638 4244 1391 Size: 711 1536 606 4072 Fragments arranged by size: 4072 1536 711 606 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3534 928 Size: 971 1635 4319 Fragments arranged by size: 4319 1635 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5116 5150 1903 Size: 34 3179 34 3678 Fragments arranged by size: 3678 3179 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4817 6027 4817 Size: 1210 5715 BclI T'GATC_A Cuts at: 1548 1548 Size: 6925 BfrBI ATG'CAT Cuts at: 2353 2353 Size: 6925 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5536 136 Size: 107 122 71 631 605 3864 1525 Fragments arranged by size: 3864 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2403 3286 6920 2403 Size: 883 3634 2408 Fragments arranged by size: 3634 2408 883 BlpI GC'TnA_GC Cuts at: 4342 4342 Size: 6925 Bme1580I G_kGCm'C Cuts at: 1790 2129 2162 2253 2483 3154 4986 6142 1790 Size: 339 33 91 230 671 1832 1156 2573 Fragments arranged by size: 2573 1832 1156 671 339 230 91 33 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6925 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4656 5614 442 Size: 416 53 83 399 24 3239 958 1753 Fragments arranged by size: 3239 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6925 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2102 5585 1239 Size: 863 3483 2579 Fragments arranged by size: 3483 2579 863 Bpu10I CC'TnA_GC Cuts at: 2193 2193 Size: 6925 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2830 3614 5050 5915 6213 2830 Size: 784 1436 865 298 3542 Fragments arranged by size: 3542 1436 865 784 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2290 5588 882 Size: 617 791 3298 2219 Fragments arranged by size: 3298 2219 791 617 BsaAI yAC'GTr Cuts at: 493 1204 4716 493 Size: 711 3512 2702 Fragments arranged by size: 3512 2702 711 BsaBI GATnn'nnATC Cuts at: 2352 6919 2352 Size: 4567 2358 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4732 5114 5749 275 Size: 53 83 186 936 3199 382 635 1451 Fragments arranged by size: 3199 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5358 5756 6030 6177 6588 5358 Size: 398 274 147 411 5695 Fragments arranged by size: 5695 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2189 2219 2429 2459 1178 Size: 30 165 30 786 30 210 30 5644 Fragments arranged by size: 5644 786 210 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2027 3546 4214 1186 Size: 841 1519 668 3897 Fragments arranged by size: 3897 1519 841 668 BseYI C'CCAG_C Cuts at: 6128 6624 6128 Size: 496 6429 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6352 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4375 5136 6164 1540 Size: 275 186 2374 761 1028 2301 Fragments arranged by size: 2374 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2102 2129 2223 4986 5071 6142 2102 Size: 27 94 2763 85 1071 2885 Fragments arranged by size: 2885 2763 1071 94 85 27 BsmI GAATG_Cn' Cuts at: 3255 4436 4529 3255 Size: 1181 93 5651 Fragments arranged by size: 5651 1181 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 2129 2162 2223 2253 2483 3154 Size: 399 312 27 33 61 30 230 671 Cuts at: 3154 3638 4244 4986 5071 6142 1391 Size: 484 606 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 671 606 484 399 312 230 85 61 33 30 27 BspEI T'CCGG_A Cuts at: 6588 6588 Size: 6925 BspHI T'CATG_A Cuts at: 3574 4814 6544 3574 Size: 1240 1730 3955 Fragments arranged by size: 3955 1730 1240 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2025 3260 3271 4280 844 Size: 1181 1235 11 1009 3489 Fragments arranged by size: 3489 1235 1181 1009 11 BsrBI CCG'CTC Cuts at: 1394 1823 2035 4812 1394 Size: 429 212 2777 3507 Fragments arranged by size: 3507 2777 429 212 BsrDI GCAATG_nn' Cuts at: 65 1248 5420 5594 65 Size: 1183 4172 174 1396 Fragments arranged by size: 4172 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5569 5756 1700 Size: 110 3759 187 2869 Fragments arranged by size: 3759 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2049 3445 96 Size: 1953 1396 3576 Fragments arranged by size: 3576 1953 1396 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6925 BssSI C'ACGA_G Cuts at: 2039 2293 2874 4985 5997 2039 Size: 254 581 2111 1012 2967 Fragments arranged by size: 2967 2111 1012 581 254 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6925 BstBI TT'CG_AA Cuts at: 5729 5729 Size: 6925 BstEII G'GTnAC_C Cuts at: 1184 2858 1184 Size: 1674 5251 BstXI CCAn_nnnn'nTGG Cuts at: 2260 2260 Size: 6925 BstYI r'GATC_y Cuts at: 1972 2403 3286 4255 4622 5007 5024 6476 6562 Size: 431 883 969 367 385 17 1452 86 Cuts at: 6562 6574 6920 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 969 883 431 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5434 Fragments arranged by size: 5434 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3615 487 Size: 683 56 2389 3797 Fragments arranged by size: 3797 2389 683 56 BtsI GCAGTG_nn' Cuts at: 4441 5235 5255 4441 Size: 794 20 6111 Fragments arranged by size: 6111 794 20 ClaI AT'CG_AT Cuts at: 1554 3318 1554 Size: 1764 5161 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6890 DraI TTT'AAA Cuts at: 2743 4229 2743 Size: 1486 5439 DraIII CAC_nnn'GTG Cuts at: 2478 3454 2478 Size: 976 5949 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5932 809 Size: 507 4616 1802 Fragments arranged by size: 4616 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4372 5261 6890 Size: 54 1425 50 275 18 2542 889 1629 Cuts at: 6890 8 Size: 43 Fragments arranged by size: 2542 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4372 1537 Size: 275 2560 4090 Fragments arranged by size: 4090 2560 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4855 1353 Size: 3502 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3612 5512 5886 6032 387 Size: 1064 138 2023 1900 374 146 1280 Fragments arranged by size: 2023 1900 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2100 2100 Size: 6925 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2102 2939 3099 3389 4988 5585 6372 Size: 393 470 837 160 290 1599 597 787 Cuts at: 6372 6640 1239 Size: 268 1524 Fragments arranged by size: 1599 1524 837 787 597 470 393 290 268 160 EcoNI CCTnn'n_nnAGG Cuts at: 3005 3503 3005 Size: 498 6427 EcoO109I rG'GnC_Cy Cuts at: 3039 4041 3039 Size: 1002 5923 EcoRI G'AATT_C Cuts at: 3743 4234 4612 3743 Size: 491 378 6056 Fragments arranged by size: 6056 491 378 EcoRV GAT'ATC Cuts at: 3192 3192 Size: 6925 FspI TGC'GCA Cuts at: 5431 5431 Size: 6925 HaeII r_GCGC'y Cuts at: 1678 6072 1678 Size: 4394 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2828 2860 5566 5598 5640 Size: 32 135 32 1256 32 2706 32 42 Cuts at: 5640 5672 1373 Size: 32 2626 Fragments arranged by size: 2706 2626 1256 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2236 4269 4515 669 Size: 988 281 298 2033 246 3079 Fragments arranged by size: 3079 2033 988 298 281 246 HindIII A'AGCT_T Cuts at: 748 3081 748 Size: 2333 4592 HpaI GTT'AAC Cuts at: 4515 4515 Size: 6925 KpnI G_GTAC'C Cuts at: 2267 4250 2267 Size: 1983 4942 MfeI C'AATT_G Cuts at: 4524 4524 Size: 6925 MluI A'CGCG_T Cuts at: 6801 6801 Size: 6925 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6039 6223 1305 Size: 4734 184 2007 Fragments arranged by size: 4734 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5103 Fragments arranged by size: 5103 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2358 3303 3441 3695 3902 4883 5242 Size: 648 1192 945 138 254 207 981 359 Cuts at: 5242 5401 518 Size: 159 2042 Fragments arranged by size: 2042 1192 981 945 648 359 254 207 159 138 MspA1I CmG'CkG Cuts at: 1606 2423 2658 3059 4341 5018 6166 6411 1606 Size: 817 235 401 1282 677 1148 245 2120 Fragments arranged by size: 2120 1282 1148 817 677 401 245 235 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5434 Fragments arranged by size: 5434 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6925 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6925 NotI GC'GGCC_GC Cuts at: 4372 4372 Size: 6925 NsiI A_TGCA'T Cuts at: 2355 2355 Size: 6925 NspI r_CATG'y Cuts at: 1132 1229 3302 3335 3801 4283 5828 1132 Size: 97 2073 33 466 482 1545 2229 Fragments arranged by size: 2229 2073 1545 482 466 97 33 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6925 PciI A'CATG_T Cuts at: 5824 5824 Size: 6925 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6925 PfoI T'CCnGG_A Cuts at: 2580 2805 2580 Size: 225 6700 PmeI GTTT'AAAC Cuts at: 4229 4229 Size: 6925 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4994 5026 6507 6539 4994 Size: 32 1481 32 5380 Fragments arranged by size: 5380 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3039 3039 Size: 6925 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6925 PsiI TTA'TAA Cuts at: 3005 4495 3005 Size: 1490 5435 PstI C_TGCA'G Cuts at: 830 4277 830 Size: 3447 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6925 PvuII CAG'CTG Cuts at: 2423 2658 3059 2423 Size: 235 401 6289 Fragments arranged by size: 6289 401 235 SacI G_AGCT'C Cuts at: 2102 2102 Size: 6925 SalI G'TCGA_C Cuts at: 1936 4267 1936 Size: 2331 4594 SanDI GG'GwC_CC Cuts at: 3039 3039 Size: 6925 SbfI CC_TGCA'GG Cuts at: 4277 4277 Size: 6925 ScaI AGT'ACT Cuts at: 5173 6885 5173 Size: 1712 5213 SfcI C'TryA_G Cuts at: 826 1045 3182 4273 5408 6089 6280 826 Size: 219 2137 1091 1135 681 191 1471 Fragments arranged by size: 2137 1471 1135 1091 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6925 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6925 SmaI CCC'GGG Cuts at: 1872 2157 4252 1872 Size: 285 2095 4545 Fragments arranged by size: 4545 2095 285 SmlI C'TyrA_G Cuts at: 820 1017 1943 2845 3629 5029 5930 6192 820 Size: 197 926 902 784 1400 901 262 1553 Fragments arranged by size: 1553 1400 926 902 901 784 262 197 SnaBI TAC'GTA Cuts at: 493 4716 493 Size: 4223 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6925 SphI G_CATG'C Cuts at: 1132 3302 4283 1132 Size: 2170 981 3774 Fragments arranged by size: 3774 2170 981 SspI AAT'ATT Cuts at: 5 52 4849 5 Size: 47 4797 2081 Fragments arranged by size: 4797 2081 47 StuI AGG'CCT Cuts at: 1517 3653 1517 Size: 2136 4789 StyI C'CwwG_G Cuts at: 513 1065 2004 2685 3034 3270 4364 513 Size: 552 939 681 349 236 1094 3074 Fragments arranged by size: 3074 1094 939 681 552 349 236 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2153 3963 4952 4969 5122 5307 5752 Size: 1255 573 1810 989 17 153 185 445 Cuts at: 5752 325 Size: 1498 Fragments arranged by size: 1810 1498 1255 989 573 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2049 3445 5171 Size: 274 80 33 51 1443 72 1396 1726 Cuts at: 5171 6883 96 Size: 1712 138 Fragments arranged by size: 1726 1712 1443 1396 274 138 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4118 4150 6885 6917 4118 Size: 32 2735 32 4126 Fragments arranged by size: 4126 2735 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6925 XbaI T'CTAG_A Cuts at: 4261 4261 Size: 6925 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3809 3809 Size: 6925 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6925 XmaI C'CCGG_G Cuts at: 1870 2155 4250 1870 Size: 285 2095 4545 Fragments arranged by size: 4545 2095 285 XmnI GAAnn'nnTTC Cuts at: 3541 5054 3541 Size: 1513 5412 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4733 276 Size: 53 83 186 936 3199 2468 Fragments arranged by size: 3199 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BplI BsiWI BsmBI BstZ17I Bsu36I FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI