(Linear) (Six-base) MAPSORT of: KIEE3287.seq Check: 6214 from: 1 to: 2220 KIEE3287.orf With 182 enzymes: SgfI * December 27, 2011 16:34 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 20 2220 Size: 20 2200 Acc65I G'GTAC_C Cuts at: 0 258 2220 Size: 258 1962 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 934 1094 1384 2220 Size: 934 160 290 836 Fragments arranged by size: 934 836 290 160 AflIII A'CryG_T Cuts at: 0 2184 2220 Size: 2184 36 AleI CACnn'nnGTG Cuts at: 0 1897 2220 Size: 1897 323 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1316 2220 Size: 1316 904 AlwNI CAG_nnn'CTG Cuts at: 0 576 2220 Size: 576 1644 ApaLI G'TGCA_C Cuts at: 0 120 2220 Size: 120 2100 ApoI r'AATT_y Cuts at: 0 270 299 889 958 1738 2220 Size: 270 29 590 69 780 482 Fragments arranged by size: 780 590 482 270 69 29 AvaI C'yCGr_G Cuts at: 0 59 105 150 2220 Size: 59 46 45 2070 Fragments arranged by size: 2070 59 46 45 BanI G'GyrC_C Cuts at: 0 154 258 1891 2220 Size: 154 104 1633 329 Fragments arranged by size: 1633 329 154 104 BanII G_rGCy'C Cuts at: 0 97 1633 2220 Size: 97 1536 587 Fragments arranged by size: 1536 587 97 BbsI GAAGACnn'nnnn_ Cuts at: 0 1529 2220 Size: 1529 691 BfrBI ATG'CAT Cuts at: 0 348 2220 Size: 348 1872 BglII A'GATC_T Cuts at: 0 398 1281 2220 Size: 398 883 939 Fragments arranged by size: 939 883 398 Bme1580I G_kGCm'C Cuts at: 0 124 157 248 478 1149 2220 Size: 124 33 91 230 671 1071 Fragments arranged by size: 1071 671 230 124 91 33 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 2220 Size: 97 2123 Bpu10I CC'TnA_GC Cuts at: 0 188 2220 Size: 188 2032 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 825 1609 2220 Size: 825 784 611 Fragments arranged by size: 825 784 611 BsaI GGTCTCn'nnnn_ Cuts at: 0 285 2220 Size: 285 1935 BsaBI GATnn'nnATC Cuts at: 0 347 2220 Size: 347 1873 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 184 214 424 454 2220 Size: 184 30 210 30 1766 Fragments arranged by size: 1766 210 184 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 22 1541 2209 2220 Size: 22 1519 668 11 Fragments arranged by size: 1519 668 22 11 BsiHKAI G_wGCw'C Cuts at: 0 97 124 218 2220 Size: 97 27 94 2002 Fragments arranged by size: 2002 97 94 27 BsmI GAATG_Cn' Cuts at: 0 1250 2220 Size: 1250 970 Bsp1286I G_dGCh'C Cuts at: 0 97 124 157 218 248 478 1149 1633 Size: 97 27 33 61 30 230 671 484 Cuts at: 1633 2220 Size: 587 Fragments arranged by size: 671 587 484 230 97 61 33 30 27 BspHI T'CATG_A Cuts at: 0 1569 2220 Size: 1569 651 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 20 1255 1266 2220 Size: 20 1235 11 954 Fragments arranged by size: 1235 954 20 11 BsrBI CCG'CTC Cuts at: 0 30 2220 Size: 30 2190 BsrGI T'GTAC_A Cuts at: 0 44 1440 2220 Size: 44 1396 780 Fragments arranged by size: 1396 780 44 BssSI C'ACGA_G Cuts at: 0 34 288 869 2220 Size: 34 254 581 1351 Fragments arranged by size: 1351 581 254 34 BstEII G'GTnAC_C Cuts at: 0 853 2220 Size: 853 1367 BstXI CCAn_nnnn'nTGG Cuts at: 0 255 2220 Size: 255 1965 BstYI r'GATC_y Cuts at: 0 398 1281 2220 Size: 398 883 939 Fragments arranged by size: 939 883 398 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 1610 2220 Size: 1610 610 ClaI AT'CG_AT Cuts at: 0 1313 2220 Size: 1313 907 DraI TTT'AAA Cuts at: 0 738 2220 Size: 738 1482 DraIII CAC_nnn'GTG Cuts at: 0 473 1449 2220 Size: 473 976 771 Fragments arranged by size: 976 771 473 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 1607 2220 Size: 1607 613 EcoICRI GAG'CTC Cuts at: 0 95 2220 Size: 95 2125 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 97 934 1094 1384 2220 Size: 97 837 160 290 836 Fragments arranged by size: 837 836 290 160 97 EcoNI CCTnn'n_nnAGG Cuts at: 0 1000 1498 2220 Size: 1000 498 722 Fragments arranged by size: 1000 722 498 EcoO109I rG'GnC_Cy Cuts at: 0 1034 2036 2220 Size: 1034 1002 184 Fragments arranged by size: 1034 1002 184 EcoRI G'AATT_C Cuts at: 0 1738 2220 Size: 1738 482 EcoRV GAT'ATC Cuts at: 0 1187 2220 Size: 1187 1033 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 823 855 2220 Size: 823 32 1365 Fragments arranged by size: 1365 823 32 HincII GTy'rAC Cuts at: 0 231 2220 Size: 231 1989 HindIII A'AGCT_T Cuts at: 0 1076 2220 Size: 1076 1144 KpnI G_GTAC'C Cuts at: 0 262 2220 Size: 262 1958 MslI CAynn'nnrTG Cuts at: 0 353 1298 1436 1690 1897 2220 Size: 353 945 138 254 207 323 Fragments arranged by size: 945 353 323 254 207 138 MspA1I CmG'CkG Cuts at: 0 418 653 1054 2220 Size: 418 235 401 1166 Fragments arranged by size: 1166 418 401 235 NsiI A_TGCA'T Cuts at: 0 350 2220 Size: 350 1870 NspI r_CATG'y Cuts at: 0 1297 1330 1796 2220 Size: 1297 33 466 424 Fragments arranged by size: 1297 466 424 33 PfoI T'CCnGG_A Cuts at: 0 575 800 2220 Size: 575 225 1420 Fragments arranged by size: 1420 575 225 PpuMI rG'GwC_Cy Cuts at: 0 1034 2220 Size: 1034 1186 PsiI TTA'TAA Cuts at: 0 1000 2220 Size: 1000 1220 PvuII CAG'CTG Cuts at: 0 418 653 1054 2220 Size: 418 235 401 1166 Fragments arranged by size: 1166 418 401 235 SacI G_AGCT'C Cuts at: 0 97 2220 Size: 97 2123 SanDI GG'GwC_CC Cuts at: 0 1034 2220 Size: 1034 1186 SfcI C'TryA_G Cuts at: 0 1177 2220 Size: 1177 1043 SmaI CCC'GGG Cuts at: 0 152 2220 Size: 152 2068 SmlI C'TyrA_G Cuts at: 0 840 1624 2220 Size: 840 784 596 Fragments arranged by size: 840 784 596 SphI G_CATG'C Cuts at: 0 1297 2220 Size: 1297 923 StuI AGG'CCT Cuts at: 0 1648 2220 Size: 1648 572 StyI C'CwwG_G Cuts at: 0 680 1029 1265 2220 Size: 680 349 236 955 Fragments arranged by size: 955 680 349 236 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 148 1958 2220 Size: 148 1810 262 Fragments arranged by size: 1810 262 148 TatI w'GTAC_w Cuts at: 0 44 1440 2220 Size: 44 1396 780 Fragments arranged by size: 1396 780 44 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2113 2145 2220 Size: 2113 32 75 Fragments arranged by size: 2113 75 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1804 2220 Size: 1804 416 XmaI C'CCGG_G Cuts at: 0 150 2220 Size: 150 2070 XmnI GAAnn'nnTTC Cuts at: 0 1536 2220 Size: 1536 684 Enzymes that do cut: AarI Acc65I AcuI AflIII AleI AlfI AlwNI ApaLI ApoI AvaI BanI BanII BbsI BfrBI BglII Bme1580I BpmI Bpu10I BpuEI BsaI BsaBI BsaXI BseRI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI BsrGI BssSI BstEII BstXI BstYI BtgZI ClaI DraI DraIII EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV Hin4I HincII HindIII KpnI MslI MspA1I NsiI NspI PfoI PpuMI PsiI PvuII SacI SanDI SfcI SmaI SmlI SphI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AgeI AhdI AloI ApaI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BglI BlpI BmgBI BmrI BmtI BplI BsaAI BsaHI BsaWI BseYI BsgI BsiEI BsiWI BsmBI BspEI BsrDI BsrFI BssHII BstAPI BstBI BstZ17I Bsu36I BtgI BtsI CspCI DrdI EaeI EagI EarI FalI FseI FspI FspAI HaeII HpaI KasI MfeI MluI MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PspOMI PspXI PsrI PstI PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI Tth111I XbaI XhoI ZraI